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Citations for Takara Bio :
3001 - 3050 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated through a NucleoSpin® RNA/Protein kit (Takara Bio USA Inc. San Jose, CA) while heart issue was processed with a RNeasy Fibrous Mini kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... The lentiviral titer was then determined using the Lenti-X p24 rapid titer kit (Takara Cat #632200). The Tau-RD-P301S-K18 FRET Biosensor CRL-3275 were exposed to active recombinant P301S-MAPT fibrils (ab246003 ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from 3-week-old seedlings with Minibest plant RNA extraction kit (Takara, 9769) and three independent biological replicates were performed ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear RNA was processed into cDNA libraries using the SMART-Seq v4 Ultra Low Input kit (Takara) using 19 PCR cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was carried out using the Mir-X™ miRNA First Strand Synthesis Kit (Takara Bio). The TB Green kit (Takara Bio ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... and reverse transcription of miRNA was performed using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa). RT-PCR amplification of mRNA was performed using the TB Green Premix Ex Taq II (Takara) ...
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bioRxiv - Neuroscience 2024Quote: ... The annealed oligonucleotides were cloned into the parent vectors using In-Fusion HD Cloning Kit (Takara 639650) and transformed into chemically competent Stbl3 E ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Ligation was performed by incubation at 16℃ for 30 minutes (DNA ligation kit Mighty mix, Takara, Japan) and the products were transformed into DH5α competent cells (Takara ...
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bioRxiv - Microbiology 2024Quote: ... The RNA (500 ng) was then reverse-transcribed using the PrimeScript 1st strand cDNA synthesis kit (Takara) following the instructions provided by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... and libraries prepared using a SMARTer ® Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian (Takara). Samples were then sequenced using an Illumina NextSeq 2000 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... the total RNA was utilized for library preparation with the SMART-Seq®-v4 PLUS Kit (Takara Bio Europe SAS ...
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bioRxiv - Bioengineering 2024Quote: ... We confirmed the cells were mycoplasma-negative using a Cycleave PCR Mycoplasma Detection Kit (Takara Bio, CY232).
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bioRxiv - Microbiology 2024Quote: The processed DNA was ready for Illumina library preparation using a SMART ChIP-seq kit (Takara, #634865) following the manufacturer’s protocol (Fig ...
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bioRxiv - Microbiology 2024Quote: ... 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA, Mountain View, CA) to identify the transcription start site (TSS ...
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bioRxiv - Neuroscience 2024Quote: ... immediately followed by reverse-transcriptions and cDNA amplification with the smart-seq HT kit (Cat# 634437, Takara). The post-amplified cDNAs (1 ng per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... The digested backbone and the two amplicons were joined by In-Fusion® HD Cloning Kit (Takara). The SFFV U3 in the pAS.SFFV.d2GFP.PRE plasmid was then exchanged for other U3 sequences by digesting the plasmid with XcmI and AscI (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 (Takara Bio USA, Inc) following the recommended protocol with minor adjustments ...
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bioRxiv - Neuroscience 2024Quote: ... 500 pg of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech) per manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... 1 ng of RNA was reverse-transcribed using the SMART-Seq v4 Low Input RNA kit (Takara). cDNA were quantified and qualified using Qubit (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... USA) was fused back using In-Fusion HD Cloning kit according to manufacturer’s protocol (#639648, Clontech, USA) (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Then 500 ng of total RNA was reverse transcribed with PrimeScript™ RT reagent Kit (Takara Bio). For quantitative PCR ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 3-week-old seedlings with Minibest plant RNA extraction kit (Takara, 9769) and three independent biological replicates were performed ...
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bioRxiv - Bioengineering 2024Quote: ... real-time quantitative PCR was performed using the TB Green Premix Ex Taq II Kit (Takara, RR820A). The primer sequences used were as follows:
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral plasmids were generated from the linearized fragments using the Takara In-Fusion Cloning kit (Takara 638947) to create pLX-TRE-EV-BSD ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA was synthesized using the PrimeScriptTM Fast RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). The qRT-PCR reactions were conducted in a final volume of 20 μL ...
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bioRxiv - Zoology 2024Quote: ... Total RNA was reverse transcribed using the PrimeScrip™ RT Reagent Kit Reverse Transcription System (TaKaRa, Japan). Transcript levels were determined using the 2× SYBR Green qRT-PCR Mix (Solarbio ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 μg RNA was reverse-transcribed into cDNA using the PrimeScript RT Reagent Kit (RR037B, Takara). RT-qPCR analysis was conducted using Hieff SYBR Green Master Mix Kit (11201ES08 ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted from 150 ul of Cas9-EDVs using the NucleoSpin RNA Virus Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... AAV virus was extracted from the cells using the AAVpro® Extraction Solution kit (Catalog# 6235, Takara). Fourteen-month-old mice (n=30 ...
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bioRxiv - Cell Biology 2024Quote: ... The absence of Mycoplasma contamination was confirmed using a PCR Mycoplasma Test Kit (Takara Bio, Kusatsu, Japan). MDCK and BEAS-2B cells were cultured in Matrigel (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... quantitative polymerase chain reaction (qPCR) analyses were performed employing a TB-Green-based PCR kit (Takara, RR82WR) in conjunction with a Real-Time PCR system (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: ... MBD2-mediated enrichment of methylated DNA was performed using an EpiXplore Methylated DNA Enrichment Kit (Takara Bio) according to the manufacturer’s instructions ...
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Developmental functions of rapgef1b in neural crest specification and presomitic mesoderm patterningbioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from total RNA using PrimeScript 1st strand cDNA synthesis kit (TaKaRa, cat. no 6110A). PCR was carried out using the prepared cDNA for amplification of zebrafish rapgef1 isoforms and rpl7 (internal control) ...
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bioRxiv - Synthetic Biology 2021Quote: ... DmrA and DmrC were amplified and then fused to each split-Cas9 (N_Cas9 and C_Cas9) using the In-Fusion HD Cloning Kit (Clontech). Links to sequences of all the generated plasmids are listed in Supplementary Table 3.
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bioRxiv - Cancer Biology 2021Quote: Full-length cDNA was synthesized from total RNA (1 μg) by SMARTer®□ PCR cDNA Synthesis kit (Clontech) according to the manufacture’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: Illumina sequencing libraries of isolated RPFs was performed with the SMARTer smRNA-Seq Kit for Illumina (Takara Bio). Library preparation was performed according to the manufacturer’s protocol with the following parameters ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed into cDNA using reagents from a Prime Script II first strand cDNA synthesis kit (Takara). Real-time PCR was performed with the reverse transcription products ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared from 8mL of RNA using the SMARTer Stranded Total RNA-seq Pico kit v2 (Clontech), per manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 500 ng total RNA was reverse-transcribed to cDNA (Prime-Script RT reagent kit, Takara Bio, Dalian, China). The parameters set for reverse transcription were as follows ...
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bioRxiv - Genetics 2021Quote: ... Strand specific RNA-seq libraries were then constructed using the SMARTer Stranded RNA-Seq HT Kit (Clontech #634839), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was amplified using PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio, Shiga, Japan) under the following reaction conditions ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated following the SMARTer® 5’/3’ RACE kit protocol (Takara Bio, Kusatsu, Shiga Prefecture, Japan).
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bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis was completed by SMART-Seq™ v4 Ultra™ Low Input RNA kit for sequencing (Clontech). Samples were sequenced on Illumina machines ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was converted to cDNA using PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara, Dalian, China). The mRNA expression levels of the Ccl9 ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA II kit (Clontech Laboratories, Mountain View, CA, USA). The quality and quantity of total RNA were measured by Nanodrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was extracted from sorted T cells using the Macherey-Nagel NucleoSpin Tissue XS kit (Takara #740901.250). DNA encoding the CAR costimulatory domain was amplified from the extracted genomic DNA using the forward primer 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNACTGGTTATCACCCTTTA CTGC-3’ (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from various tissues using the MiniBEST Plant RNA Extraction Kit (Takara Bio, Beijing, China). First-strand cDNAs were synthesized using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech ...
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bioRxiv - Molecular Biology 2022Quote: The RNA samples extracted from the fruit flesh were reverse transcribed using the PrimeScript RT-PCR Kit (TaKaRa) and the oligo(dt)20 primer ...
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bioRxiv - Microbiology 2022Quote: ... The first-strand cDNA was synthesized using reverse transcriptase from the PrimeScript RT reagent kit (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... were purchased from Biomatik and cloned into BstBI-digested PBRM1-TetO-FUW using the ligation-free In-Fusion HD cloning kit (Takara). The construct was packaged into lentivirus using HEK293T cells and delivered into target cells together with pLenti CMV rtTA3 Hygro (w785-1 ...