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Citations for Takara Bio :
2951 - 3000 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared at La Jolla Institute for Immunology using SMARTer Stranded RNA-Seq kit (Takara 634962) and were sequenced using an Illumina HiSeq2500 in Rapid Run Mode with paired end 50 reads.
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription of RNA was carried out using the PrimeScript RT reagent Kit with gDNAEraser from TAKARA. The resulting cDNA from reverse transcription was then amplified by PCR and digested using PstI ...
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bioRxiv - Evolutionary Biology 2023Quote: The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Microbiology 2023Quote: ... DNA in the supernatant was purified using the NucleoSpin Gel & PCR kit (Takara Bio, Kusatsu, Shiga, Japan). Sequencing libraries were prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... the RNA was reverse transcribed using the PrimeScriptII 1st strand cDNA Synthesis Kit (Takara bio, Shiga, Japan). Quantitative RT-PCR was performed using SYBR Premix EX TaqII (Takara bio ...
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bioRxiv - Cancer Biology 2023Quote: These RNA samples were used for cDNA synthesis using PrimeScript 1st strand cDNA synthesis kit (Takara, Japan) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All cloning experiments were performed using an In-Fusion HD Cloning Kit (Takara Bio Inc., Shiga, Japan), and plasmid sequences were confirmed by DNA sequencing (at the Core Instrumentation Facility ...
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bioRxiv - Immunology 2022Quote: ... and cDNAs were synthesized using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... gDNA from all cells were isolated using the Machery Nagel L Midi NucleoSpin Blood Kit (Clontech, 740954.20). Modifications to the manufacturer’s instructions were added as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq ...
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bioRxiv - Neuroscience 2022Quote: ... and then cloned into pGEMHE using In-Fusion HD Cloning Kit according to the manufacturer’s instructions (Clontech).
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bioRxiv - Cell Biology 2022Quote: ... and mRNA reverse transcription was performed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). The qPCR was performed using SYBR Green qPCR Master Mix (Takara ...
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bioRxiv - Genomics 2022Quote: ... and first-strand cDNA was synthesized using a PrimeScript RT Reagent Kit (Catalog No. RR037A, TaKaRa, Tokyo), which was used for subsequent transcriptomic analyses ...
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed using the SYBRGreen included in the BacPAK™ qPCR titration kit (Clontech, USA) and a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for cDNA cloning was performed with PrimeScript™ II 1st Strand cDNA Synthesis kit (Takara), Gibson assembly was performed with NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from total RNA using PrimeScript™ reverse transcriptase reagent Kit (Perfect Real Time) (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-Up kit (Takara Bio, Kusatsu, Shiga, Japan), and 600 ng-1 ug of DNA templates were added to set up in vitro transcription reactions ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using the SYBR Premix Ex Taq kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HuEpi library was sequenced and contains all 5,309 sgRNAs included in the synthesis (GSE215430).
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bioRxiv - Molecular Biology 2022Quote: ... The in vitro assembly of PCR products was performed by using In-Fusion HD cloning kit (Takara). The assembled plasmids were amplified by transformation of Stellar Competent Cells (Takara ...
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bioRxiv - Neuroscience 2023Quote: Neurons were transfected at day in vitro (DIV) 7 using CalPhos Mammalian Transfection Kit (Takara Bio, 631312). Transfection solution was prepared by adding 1.5 µg DNA per construct (3 µg in total ...
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bioRxiv - Plant Biology 2023Quote: ... Poly-A tail enriched cDNA libraries were prepared using the SMART-Seq HT Kit (Takara Bio, Japan), quality checked using the TapeStation DNA ScreenTape (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding cDNA was transcribed using a Fast Quant RT Kit with gDNA (TaKaRa Bio, Shiga, Japan). The cDNA was used as a template for RT-PCR assays ...
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bioRxiv - Genomics 2023Quote: ... The total RNA library was prepared using SMARTer® Stranded Total RNA-Seq Kit v2 (TAKARA 634413). The rest of the nuclei were reverse crosslinked ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined using the Lenti-X™ p24 Rapid Titer Kit (Cat: 632200, Clontech).
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bioRxiv - Molecular Biology 2023Quote: ... total RNA was used for cDNA synthesis with the Mir-X miRNA First-Strand Synthesis Kit (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... from which cDNA was obtained by reverse transcription using the PrimeScript RT Reagent Kit (TaKaRa, Kusatsu, Japan). Transcript levels of RvNaRL were examined using a real-time PCR system (LightCycler 96 system ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the PimeScript RT-PCR kit (TAKARA) and analyzed by qPCR on LightCycler96 system (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA-seq libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared from 10Dng of RNA from each sample using the SMART-Seq v4 kit (Takara). One VTA male sample (VTA_MW_3 – see page 9 and 10 of supplementary data 1 ...
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bioRxiv - Microbiology 2023Quote: Amplification and detection were performed using the One Step PrimeScript™ RT-PCR Kit (RR064A, Takara Bio) with the following primers targeting the IAV M segment ...
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bioRxiv - Biochemistry 2023Quote: The plasmids of EcOpgD and EcOpgG mutants were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was reverse transcribed with the PrimeScript™RT Reagent Kit (Takara Bio Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... DNA in the supernatant was purified using the NucleoSpin Gel & PCR kit (Takara Bio, Kusatsu, Shiga, Japan). Sequencing libraries were then prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were cloned into pHAGE-EF1a-Cre-w41 plasmid using an infusion cloning kit (Takara, 638947). Lentiviral vectors were transfected into the packaging HEK293 cell line ...
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bioRxiv - Cell Biology 2024Quote: ... we amplified EF1a promoter region from pEP4 E02S CK2M EN2L by PCR and cloned into pHR_SFFV_LaG17_synNotch_TetRVP64 using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Genetics 2024Quote: ... cDNA was generated from 1μg of total RNA using the PrimeScriptTM RT reagent kit (TaKaRa, Shiga, Japan) for the quantitation of rRNA by quantitative real-time PCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cDNA was reverse transcribed by using a PrimeScript RT Reagent Kit Perfect Real Time (TaKaRa Bio Inc.). Real-time PCR was performed using a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA (1 μg) was reverse transcribed into cDNA using PrimeScript RT reagent kit (Takara Bio, Japan), and expression was quantified using the TB Green® Premix Ex Taq™ II (Takara) ...
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bioRxiv - Cell Biology 2024Quote: Paraffin sections were used for H/E staining and ALP staining with a commercial staining kit (TaKaRa, TRACP & ALP double-stain kit ...
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bioRxiv - Genetics 2024Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a PrimeScript RT reagent Kit (Cat#RR047B, TaKaRa) and was used as a template to perform qPCR in TB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Biophysics 2024Quote: ... and cDNA was synthesized using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA was synthesized from 1 μg RNA using PrimeScriptTM RT Master Mix kit (Takara, Cat#RR036A) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... the first strand cDNA was prepared using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China).
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using TB Green Premix Ex Taq Kit (Takara) on QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...