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Citations for Takara Bio :
3101 - 3150 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... mRNA library preparation was performed following manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit, TAKARA Bio). Final samples pooled library prep was sequenced on Nextseq 500 ILLUMINA with HighOutPut cartridge (2×400Millions of 75 bases reads) ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 250 mg of sponge sample using the Nucleospin Soil DNA extraction kit (Takara Bio) and was submitted to the Baylor College of Medicine’s Alkek Center for Metagenomics & Microbiome Research for high-throughput sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were cloned into the modified pET28 vector using the In-Fusion HD Cloning Kit (Clontech) to generate pET28M-MpaKAI2A and pET28M-MpaKAI2B ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 1 μg of total RNA using the PrimeScript™ RT Reagent Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... poly-A libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (R400675, Takara, Mountain View, CA, USA). Each library was normalized to 1.5 nM ...
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bioRxiv - Cell Biology 2022Quote: ... Titer of the lentiviruses was measured with a lentivirus titration kit (Takara Bio USA, San Jose, CA, USA). The same multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634413). All samples were sequenced on Illumina NextSeq500 (Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT Reagent Kit (TaKaRa, RR037A) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... IGK and IGL 5’RACE AIRR-seq libraries were generated using the SMARTer Mouse BCR Profiling Kit (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... Base substitutions were induced in the pMXs vector with the PrimeSTAR® Mutagenesis Basal Kit (TaKaRa Bio Inc.) before transfection into Plat-E cells to create ALK-TKIs resistant mutants.
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bioRxiv - Genetics 2022Quote: cDNA was generated from 1 μg of total RNA by using the PrimeScript RT Reagent Kit (Takara, Japan) in accordance with the manufacturer’s protocol as previously described [21] ...
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bioRxiv - Biochemistry 2022Quote: ... and the DNA was extracted and then DNA libraries were prepared with Smarter Thruplex DNAseq Kit (Clontech, R400674) following the manufacturer’s protocols with amplification for 10-13 circles ...
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bioRxiv - Microbiology 2022Quote: ... Release of LDH into the culture supernatant was quantified after infection using an LDH Cytotoxicity Detection Kit (Clontech). LDH release was normalized to mock-infected cells and cells treated with 1% Triton to establish maximum LDH release ...
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bioRxiv - Developmental Biology 2022Quote: Purified total RNA was reverse transcribed into cDNA by PrimeScript™ 2 1st strand cDNA Synthesis Kit (TaKaRa). The target gene fragments (Brachyury ...
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bioRxiv - Genomics 2020Quote: ... full-length cDNA synthesis from poly-A containing transcripts was performed using the Clontech SMARTer PCR kit (Takara). The resulting cDNA was PCR amplified to generate 1-2ug cDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was first amplified by reverse transcription using the PrimeSCript RT reagent kit (Takara Bio, Inc, Otsu, Japan). Then the relative expressions were measured with TaqMan MicroRNA Assay kit (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... and cDNA libraries were constructed for RNA sequencing by using a SMART-Seq HT Kit (Takara, Shiga, Japan) and a Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid constructions were performed with standard restriction enzyme cloning or In-fusion HD Cloning kit (Takara Bio USA) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... polyA enriched RNAs were subjected to RNA-sequencing library preparation using the SMART-Seq v4 Kit (SSv4; Takara) and sequenced using a paired-end 150bp configuration with 30M supporting reads per sample ...
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bioRxiv - Microbiology 2020Quote: ... The pSMART BAC-T7-scv2-replicon-Amp DNA was prepared using NucleoBond BAC 100 kit (Takara Cat# 740579). A fragment containing the RdRp D760N D761N mutation sequence was amplified by PCR using primers 5’-CGTAAACATTTCTCAATGATGATACTCTCTAACAATGCTGTTGTGTGTTTCAATAG-3’ and 5’-CAAAGAACACAAGCCCCAACAGCCTGTAAGACTGTATGCGGTGTGTACATAGC-3’ ...
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Airway Gene-Expression Classifiers for Respiratory Syncytial Virus (RSV) Disease Severity in InfantsbioRxiv - Immunology 2020Quote: ... 1 ng of total RNA was amplified using the SMARter Ultra Low amplification kit (Clontech, Mountain View, CA) and libraries were constructed using the NexteraXT library kit (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... One microgram of RNA was reverse transcribed into cDNA using a PrimeScript™ RT reagent Kit (TaKaRa, RR037A) in a 20 μl reaction ...
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bioRxiv - Plant Biology 2021Quote: ... URA3: GAL1UAS–Gal1TATA–LacZ MEL1) via the Yeastmaker™ Yeast Transformation System 2 kit (Clontech, Mountain View, USA). An agarose gel image of the cDNA library is shown in Fig ...
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bioRxiv - Immunology 2020Quote: ... 0.7-1 ng total RNA with an integrity index from 8.3 to 9.9 was amplified using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), with 12 cycles of amplification ...
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bioRxiv - Genomics 2021Quote: Sequencing libraries were generated using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio, #634413). The library preparation protocol started from 6 μL eluate for the biofluid samples and 100 ng (or 10 ng ...
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bioRxiv - Neuroscience 2021Quote: ... Full length cDNAs were generated using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis for Next-Generation Sequencing (NGS) was performed by SMARTer Stranded RNA-seq kit (Clontech Laboratories, USA) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed using a PrimeScript™ RT Reagent Kit (Takara Bio, Mountain View, CA 94043 USA). qPCR was performed with gene specific primers and SYBR Green (iTaq Universal SYBR Green Supermix ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Both missing ends could be recovered by RACE PCR (SMARTer RACE kit, Takara Bio, Mountain View, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... RNA was then reverse transcribed into cDNA by PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio Inc.). Gene mRNA levels were determined using SYBR green dye on Applied Biosystems Q6 Real-Time PCR cycler (Thermo ...
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bioRxiv - Biochemistry 2021Quote: The mutant cDNAs of bovine rhodopsin (accession no. AB062417) were constructed using an In-Fusion cloning kit (Clontech). The wild-type and mutant cDNAs of bovine rhodopsin were inserted into the mammalian expression vector pUSRα 24 or pCAGGS 25 ...
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bioRxiv - Immunology 2022Quote: ... cDNA generation and amplification were performed with SMART-Seq v4 ultra Low Input RNA kit (Takara Bio 634891). Amplified cDNA was purified using AMPure XP Beads (Beckman Coulter A63881) ...
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bioRxiv - Plant Biology 2021Quote: ... one microgram of DNase-treated RNA was used for reverse transcription using a PrimerScript RT reagent kit (Takara) and a mix of oligo poli-dT and random hexamers ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNAs were synthesized using the SMARTer RACE cDNA Amplification Kit (Cat. No. 634858; Takara, Mountain View, CA, USA). In silico predicted pou-iv gene sequence was retrieved from the Joint Genome Institute genome database (Nematostella vectensis v1.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... lncRNA NEAT1 and MMP2 mRNA were reversed transcribed into cDNA using PrimeScript RT Reagent Kit (Takara, Dalian, China), and miR-1299 was reversed transcribed into cDNA using TaqMan microRNA Reverse Transcription Kit (Invitrogen ...
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bioRxiv - Genomics 2020Quote: First strand cDNA synthesis was performed on ~1μg RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) followed by PCR amplification with PrimeSTAR GXL DNA Polymerase (Clontech ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted RNA was reverse transcribed using a PrimeScript RT-PCR kit with DNase I (Perfect Real Time) (Takara) in accordance with the accompanying protocol ...
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bioRxiv - Immunology 2021Quote: ... Full-length cDNA was generated using the Clontech SMARTer v4 kit (Takara Bio USA, Inc., Mountain View, CA) prior to library generation with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Immunology 2020Quote: ... and purification of cDNA was performed using either the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA ...
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bioRxiv - Immunology 2020Quote: cDNA from sorted cells was generated using the SMART-Seq v4 Ultra low input RNA kit (Clontech # 634890) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated from 110 ng of total RNA using the PrimeScript™ RT reagent Kit (Takara, Japan) with a combination of both oligo-dT and random hexamers following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Samples were prepared with SMARTer® Stranded Total RNA-Seq – Pico Input Mammalian library preparation kit v2 (Takara). Protocol included cDNA synthesis from mRNA ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated from 110 ng of total RNA using the PrimeScript™ RT reagent Kit (Takara, Japan) with a combination of both oligo-dT and random hexamers following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and was reverse transcribed using a PrimeScript ® RT reagent kit with gDNA Eraser (RR047A; TaKaRa, Dalian, China). qRT-PCR was performed with SYBR Green master mix (DRR420A ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA products were amplified by qPCR using the SYBR®Premix Ex Taq TM kit (Takara, RR420A, Japan) in an Applied Biosystem 7500 Fast Real-Time PCR System under standard PCR conditions ...
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bioRxiv - Genomics 2021Quote: We then performed first-strand synthesis using the SMARTer Stranded Total RNA Kit V2-Pico Input Mammalian (Takara). Following purification with RNAclean XP (Beckman Coulter) ...
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bioRxiv - Genetics 2020Quote: ... and reverse transcription (RT) was performed using PrimeScript RT reagent Kit with gDNA Eraser (TAKARA Bio, Kusatsu, Japan). Primers were designed to specifically amplify cDNA from transfected vectors and endogenous RPGR from 293T and ARPE-19 cells respectively ...
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bioRxiv - Neuroscience 2020Quote: ... Target sites were amplified from tail genomic DNA by PCR using the Ex Taq Polymerase kit (Takara RR001A) with primers listed in Supplemental Table 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA libraries were prepared using SMARTer kit (Clontech Laboratories, Inc. A Takara Bio Company, Mountain View, CA, USA) followed by the Nextera protocols (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... and in-chip reverse transcription PCR were performed using a 3’ DE Chip and Reagent kit (Takara Bio) according to the manufacturer’s instructions ...