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Citations for Takara Bio :
2901 - 2950 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... RNA was extracted and libraries prepared using SMARTer Stranded RNA-Seq Kits according the manufacturer’s protocols (Takara) by the Integrated Genomics Operation (IGO ...
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bioRxiv - Immunology 2023Quote: ... cDNA libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit (v2) – Pico Input Mammalian (Clontech) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from the plume of six individuals using RNAiso Pure RNA Isolation Kit (Takara, Japan) and assessed for quality using NanoVue Plus spectrophotometer (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The PCR mix (15 ul) contained One step TB Green RT-PCR kit (RR096A, Takara Bio Inc.), each primer ...
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bioRxiv - Immunology 2023Quote: ... directly into reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... First-strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech) with specific oligo(dT ...
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bioRxiv - Neuroscience 2023Quote: ... This band was cut from the gel and digested using the Takara Gel Purification kit (Takara #740609.50).
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using Smarter Stranded Total RNA-Seq Kit - Pico Input Mammalian (Takara Bio, #635005). For committed cells ...
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bioRxiv - Molecular Biology 2022Quote: ... the total RNA was reverse transcribed to cDNA using the Prime ScriptTM RT reagent kit (TaKaRa Biotechnology). qPCR was performed using the SYBR Premix Ex Taq II Kit (TaKaRa Biotechnology ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The downstream genomic region was then amplified using the primer set MpHEC1_CDS_IF_F and MpHEC1_3UTR_IF_R2 and integrated into the first binary plasmid with an In-Fusion HD Cloning Kit (Clontech) using the BamHI and AscI sites ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were assembled into pEMC vector one-by-one using in-fusion HD cloning kit (Takara), generating pEMC-eGFP-MBaMV ...
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bioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HIVDEP library was sequenced and contains all 4,191 sgRNAs included in the synthesis (GEO Dataset ...
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bioRxiv - Molecular Biology 2022Quote: ... rAAVs were purified using AAVpro® Purification Kit for All Serotypes (Cat # 6666, Takara Bio USA, CA) and each of the purified rAAVs were tested in separate transwell BBB cultures with hCMEC/D3 cells and primary astrocytes at MOI of 1e4 ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized with 1 μg of total RNA using the Prime ScriptTM RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation ...
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bioRxiv - Bioengineering 2024Quote: ... Viral genome number was quantified by real-time PCR with AAVpro Titration Kit Ver.2 (Takara, 6233) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... Both reagents were included in SMART-Seq Single Cell PLUS kit (Takara Bio, San Jose, CA, U.S.). Immediately after sampling in lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were prepared using the SMARTer v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA). Sequencing was performed on NovaSeq S4 150PE platform (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated into the digested lentiCRISPRv2 backbone using the DNA ligation kit (Mighty Mix) (Takara, Cat# 6023). The ligated DNA was then transformed into One Shot Stabl3 competent cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The viral titer was measured using a Lenti-X qRT‒PCR Titration Kit (#631235, TaKaRa, Shiga, Japan).
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bioRxiv - Neuroscience 2024Quote: ... The target genes were amplified by qPCR according to the instructions of kits (Cat.no. 638315 Takara, Japan), reversely ...
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bioRxiv - Genomics 2024Quote: ... Library construction was performed based on the manufacturer’s recommendation for SmarterStranded V2 kit (Takara Bio, California, USA). The final library quantity was measured by KAPA SYBR FAST qPCR and library quality was evaluated by TapeStation D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... and the YFP cDNA was assembled using an In-fusion HD cloning kit (Takara-Bio, Shiga, Japan). (2 ...
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bioRxiv - Microbiology 2024Quote: ... The reverse transcription was performed using the PrimeScript RT reagent kit (Takara, Saint-Germain-en-Laye, France) with a primer oligo-d(T)-AP (5’ GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTv 3’) ...
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bioRxiv - Immunology 2023Quote: ... Illumina libraries were constructed using SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (TaKaRa, Japan). RNA-seq libraries were sequenced at read lengths 2 × 151bp on the Illumina Novaseq 6000 (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... or 5 ng of total RNA (with a SMARTer Stranded Total RNA-Seq Kit v3; Takara Bio) was used.
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Each cDNA was generated using a Clontech SMART-Seq HT Kit (Takara Clontech, Mountain View, CA, USA), and each library was prepared using a Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Genetics 2023Quote: ... The PCR produc t was purified with a PCR purification kit (Takara Biomedical Technology, Bei jing, China) and was cloned into a pMDTM18-T vector (Takara Biomedical Tec hnology ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNA-seq libraries were generated using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio, Cat. # R400674). Each library was indexed using the DNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: All viruses and lentiviruses were produced by transfecting Hek293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) according to the manual in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... pseudonana (PtPyShell1, TpPyShell1, and TpPyShell2) were determined by RACE using a SMARTer RACE 5’/3’ kit (TaKaRa). Sequences were amplified by PCR and cloned into pPha-T1 or pTha-NR vectors containing a fragment of enhanced GFP by a seamless ligation cloning extract method (Motohashi ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized from 500 ng RNA using a Primescript RT Reagent Kit (Takara Bio, Shiga, Japan) with random hexamer and oligo(dT ...
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bioRxiv - Immunology 2023Quote: ... RNA was then amplified using SMART-Seq v.4 Ultra Low Input RNA kit (Clontech, cat. 63488). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... cDNAs were prepared with Clontech Smart-seq v4 ultra-low kit (Cat# 634888, Takara Bio, Shiga, Japan). PolyA tail enrichment was done using NEBNext® Poly(A ...
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bioRxiv - Physiology 2023Quote: ... Full-length cDNA was generated using the TaKaRa SMART-Seq v4 Ultra Low kit (TaKaRa Cat # 634889). mRNA-Seq libraries were then prepared using the Illumina Nextera XT DNA Library Preparation kit (Illumina Cat # FC-131-1024) ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs efficiency was assessed in vitro using the sgRNA In vitro Transcription and Screening kit (Clontech, 631439) following the manufacturer’s protocol ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Linearized pMK1334 vector was purified using NucleoSpin Gel and PCR clean-up kit (Takara Bio, Cat#740609.50). Top and bottom oligos of non-targeting and targeting sgRNAs were annealed ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was performed using a one-step PrimeScript RT-PCR kit (Takara Bio, Cat. RR057B, Japan) as per the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... Resulting linear amplification products were pooled and purified using the NucleoSpin Gel and PCR Purification kit (Takara) with modified protocols for ssDNA (1:2 NTI buffer dilution) ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was prepared using the Prime Script RT Reagent Kit (Takara Bio, Shiga, Japan; RR047B) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... using the Takara One Step TB Green PrimeScript™ RT-PCR kit (Takara Bio Inc., Shiga, JP) and the Roche LightCycler® 96 real-time PCR instrument (Roche Applied Science ...
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bioRxiv - Microbiology 2023Quote: ... cDNA reactions were performed according to the manufacturer’s instructions of the PrimeScript™ RT Reagent Kit (Takara) using primers specifically targeting the U6 and scaffold region (forward primer 5’-CCGACTCGGTGCCACTTTTT-3’ ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was prepared using the SMART-Seq Ultra Low Input RNA Kit for Sequencing (Takara Bio). RNA-seq libraries were constructed using the NexteraXT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Biochemistry 2023Quote: The PrimeSTAR® MAX DNA Polymerase and In-Fusion® HD Cloning Kit were purchased from Takara Bio Inc ...
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bioRxiv - Cell Biology 2023Quote: RNASequencing libraries were generated using the SMARTer Stranded Total RNA-Seq v2 – Pico Input Mammalian kit (Takara). 100ng of RNA was used as input following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Sequencing libraries were generated by SMARTer Stranded Total RNAseq Kit – Pico Input Mammalian V3 (Takara Bio, #634485) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA was synthesized using PrimeScript RT reagent Kit (Perfect Real Time, TAKARA BIO Inc. Shiga, Japan). The mouse aorta was homogenized by TissueLyser II (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... and SHANK3-3D (T551D/S694D/S781D) were generated with a PrimeSTAR Mutagenesis Basal Kit (Takara, Shiga, Japan). pGEX-14-3-3ζ ...