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Citations for Takara Bio :
251 - 300 of 1122 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... which encodes the chaperone protein tag (TaKaRa Bio, Shiga, Japan), following the manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: ... behind the myc-tag in the BamHI-digested pCMV-Myc-HIF-1α DM(PP/AA) construct (Berra et al,2003) using In-Fusion HD Cloning (Clontech). Then ...
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bioRxiv - Cell Biology 2020Quote: The full-sized Not (aa 496) was PCR-amplified using primers 5’-ttgaattcatgtccgagacgggttgtc-3’ and 5’-ttgtcgacttactcgtattccagcacatt-3’ and subcloned into pGBT9 vector (Clontech) in frame with DNA-binding domain of GAL4 using restriction sites EcoRI and Sal1.
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bioRxiv - Cell Biology 2020Quote: The full-sized CP190 (aa 1096) was PCR-amplified using primers 5’-ttcccgggcatgggtgaagtcaagtccg-3’ and 5’-tttggaggagctatatttactaagatct-3’ and subcloned into pGAD424 vector (Clontech) in frame with activation domain of GAL4 using restriction sites SmaI and BamHI ...
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bioRxiv - Cell Biology 2019Quote: ... The sequences encoding murine talin1 head domain (THD, aa 1-433) or full-length talin1 were cloned into pEGFP-N1 (Clontech). The sequences encoding human Rap1a(Q63E ...
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bioRxiv - Molecular Biology 2022Quote: ... Mammalian expression vector for Flag-EBNA1 contained B95-8 EBNA1 lacking the GA repeats (aa 101-324) under the control of CMV-3XFLAG promoter in a plasmid derived from pREP10 (Clontech) containing ...
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bioRxiv - Genetics 2022Quote: ... then washed in water and re-suspended in 125-250mL -leu - his galactose liquid culture (Takara Bio Minimal SD Bases, Takara Bio DO Supplement -His/-Leu). Cultures were grown to saturation ...
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bioRxiv - Microbiology 2021Quote: ... Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA, Mountain View, CA, USA) using the primers indicated in Table 1 after cDNA synthesis using random hexamer primers and Roche AMV reverse transcriptase (10109118001 Roche ...
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bioRxiv - Microbiology 2020Quote: ... The Spike ectodomain was purified by immobilised metal affinity chromatography using Talon resin (Takara Bio) charged with cobalt followed by size exclusion chromatography using HiLoad 16/60 Superdex 200 column in 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The Spike ectodomain was purified by immobilized metal affinity chromatography using Talon resin (Takara Bio) charged with cobalt followed by size exclusion chromatography using HiLoad 16/60 Superdex 200 column in 150 mM NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-E-cadherin rat monoclonal antibody (Takara, M108, 1:500), anti-GFRα1 goat polyclonal antibody (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... Rabbit polyclonal antibodies used were: HECD1 (anti-E-cadherin; Takara), anti-Melan-A (ab15468 ...
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bioRxiv - Physiology 2023Quote: ... and rat anti-E-cadherin (1:200, Takara Bio, M108). Next ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used were CDH1 (E-CADHERIN) (M106, Takara Biosciences) for glandular and luminal epithelial staining ...
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bioRxiv - Biochemistry 2019Quote: ... Western blotting was performed with anti-6×His mouse mAb (Clontech), with secondary IRDye 800CW goat anti-mouse (LI-COR ...
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bioRxiv - Neuroscience 2021Quote: ... His-tagged Cbln1 were purified by Talon metal affinity resin (Clontech) and dialyzed against HBSS ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Capturem™ His-Tagged Purification Maxiprep Kit (Takara). The binding reaction was performed in 20 μL binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant (800 µL) was incubated with 30 µL of Talon beads (Takara Bio) at 4°C for 1 hour with continuous shaking ...
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bioRxiv - Microbiology 2020Quote: ... Chemically competent Escherichia coli DH5α cells (Takara, Daliang, China) were regularly used for cloning and were cultured at 37 °C in Luria-Bertani medium supplemented with the appropriate antibiotics.
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bioRxiv - Microbiology 2019Quote: ... and transformed into Escherichia coli DH5α competent cells (Takara Biotechnology Co. ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... coli using the NucleoSpin Plasmid miniprep kit (Takara 740588). Transformants were sequenced to confirm successful cloning ...
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bioRxiv - Bioengineering 2019Quote: ... coli DH5α (TaKaRa, hsdR, recA, thi-1, relA1, gyrA96). E ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: Rng2CHD was expressed in BL21 Escherichia coli (Takara Bio) according to the instructions provided by the manufacturer of pColdI ...
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bioRxiv - Pathology 2021Quote: ... coli according to the manufacturer’s instructions for PcoldI (Takara). Briefly ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells: Stellar competent cells (Takara Bio, Cat#636763), chemically competent DH5α cells or NEB 10-beta competent cells (NEB ...
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bioRxiv - Microbiology 2019Quote: ... coli K12 strain HST08 Premium (Takara Bio, Shiga, Japan) was used for cloning ...
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bioRxiv - Microbiology 2019Quote: ... coli DH5α with the RNAiso Plus kit (Takara, Japan). RNA deep-sequencing was performed by a commercial sequencing company (Novogene ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli Stellar cells (Clontech Laboratories, Inc., Mountain View, CA) and PCR identified clones were confirmed by DNA sequencing.
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bioRxiv - Bioengineering 2023Quote: ... coli DH5α competent cells (Takara Bio Inc., Otsu, Japan), and then extracted and purified using a Nucleobond xtra maxi plus EF (Takara bio) ...
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bioRxiv - Cell Biology 2022Quote: ... E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio) per the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... listed in table S2) and inserted the 2xFlag_2xStrep_MST2 constructs (wild type or del_EDG) in the inducible vector pLVX (Clontech, #631847).
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR products of stage 2 ray florets using c25599_g2_i1 Fill-F and c25599_g2_i1 Fill-R primers (Table S2) were digested with SacI (Takara Bio Inc). Reaction mixture was consisted of 2.5 µL of PCR product ...
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bioRxiv - Biochemistry 2021Quote: ... RNC 82-aa C34A with [SG]5 or [SG]10 repeats were constructed by the Prime STAR MAX (Takara Bio Inc., Japan) method using appropriate primers (Table 1).
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bioRxiv - Cell Biology 2020Quote: Mouse monoclonal E-cadherin antibody (HECD-1) was purchased from Takara Bio Inc ...
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bioRxiv - Microbiology 2020Quote: ... D and E using high fidelity PrimeSTAR Max DNA Polymerase (Takara). T7 promoter was introduced upstream of 5’ UTR of SARS-CoV-2 genome in fragment A ...
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bioRxiv - Microbiology 2019Quote: ... IN and CCD proteins were loaded onto a His-TALON column (TAKARA), and the columns were washed with IN wash buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were purified using Capturem™ His-Tagged Purification kit (Takara Bio), then dialyzed by PBS buffer overnight ...
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bioRxiv - Microbiology 2019Quote: ... The resulting fragment was cell-free cloned into pNI-His (Takara Bio) for expression in B ...
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bioRxiv - Biophysics 2021Quote: ... His-tagged QUEEN protein was bound to TALON Metal Affinity Resins (Clontech) at 4°C ...
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bioRxiv - Microbiology 2021Quote: abTse3-His was cloned into vector pET28a by In-Fusion (Takara Bio). E ...
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bioRxiv - Microbiology 2020Quote: ... and spotted on SD supplemented with -Leu/-Trp/-His DO supplement (Clontech) (SD-Leu-Trp-His ...
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bioRxiv - Molecular Biology 2021Quote: ... transferred onto nitrocellulose and detected with either mouse anti-6×His (Clontech) at 0.25 µg/ml or rabbit antibody against calmodulin binding peptide Calmodulin Binding Peptide (GenScript ...
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bioRxiv - Microbiology 2023Quote: ... embedding and immunogold staining using a 6×His monoclonal antibody (Takara Bio) diluted 1/5000 as the primary antibody ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tagged proteins were purified with His60 Ni Superflow resin (TaKaRa; 635677) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... coli using a NucleoBond Midi or Maxi EF kit (Clontech) and eluted to a concentration of 5–10 µg/µL in TE.
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bioRxiv - Genomics 2022Quote: ... coli by using a NucleoBond Xtra BAC kit (Takara, 740436.25) for delivery into mESCs ...
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bioRxiv - Cell Biology 2020Quote: ... coli strain C43(DE3) using a pColdII vector (Takara Bio) (pColdII-His-GST-TEVsite-H1.8) ...
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bioRxiv - Genomics 2021Quote: ... coli (Supplementary Information) and subcloned into pColdI (Takara Bio Inc.) for protein expression in E ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21(DE3)-pGro7/GroEL (Takara Shuzo Co., Kyoto, Japan) grown in ZYP-5052 media were used for the expression of the recombinant protein ...
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bioRxiv - Molecular Biology 2020Quote: ... Coli cells (Takara Bio USA Inc., Mountain View, CA, USA) by incubation on ice for 30 min ...