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Citations for Takara Bio :
201 - 250 of 1122 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Klotho genotypes were determined by semi-quantitative PCR using LA-Tag DNA polymerase and TaKaRa buffer with Mg+2 (TaKaRa Shuzo, Tokyo, Japan) as follows ...
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bioRxiv - Immunology 2023Quote: ... domain followed by an Avi-Tag(76) and a hexahistidine tag was cloned into a mammalian protein expression vector (pADD2) by In-Fusion (Takara Bio).
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies against E-cadherin (clone ECCD2 Takara, M108), c-Jun (CST #9165) ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-E-Cadherin (CDH1, Takara M108, 1:200), anti-GATA6 (R&D ...
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bioRxiv - Cell Biology 2023Quote: ... and GFP-IDR-C (384-413 aa) were amplified via PCR and cloned into the pColdI vector (Clontech) at BamHI site ...
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bioRxiv - Plant Biology 2022Quote: ... His (SD/-Trp/-Leu/-Ade/-His) and with sprayed 100 µl of 4 mg/ml X-α-Gal (Takara Bio, CA, USA) in dimethylformamide on plates (SD/-Trp/-Leu/-His/X-α-Gal) ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids for other His-mCherry or His-GFP fusion proteins were constructed using in-Fusion HD Cloning Kit (Takara Bio, Inc.). All mCherry/GFP fusion proteins were expressed in E ...
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bioRxiv - Plant Biology 2020Quote: ... Ade and His but containing X-α-gal (Clontech) and 10 mM 3-amino-1,2,4-triazole (3-AT) ...
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bioRxiv - Biochemistry 2019Quote: ... a 96-well Capturem His-tagged purification kit (Clontech) was used according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... and –His/–Trp/–Ura dropout supplement (Clontech, cat. 630424). Colonies were grown in SD/–His/–Trp/–Ura medium for one night at 30 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... SD-Trp-Ura or SD-His-Leu (Clontech Takara).
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bioRxiv - Synthetic Biology 2023Quote: ... SD-Trp-Ura or SD-His-Leu (Clontech Takara).
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bioRxiv - Microbiology 2020Quote: ... coli Top10 or Stellar cells (Takara Bio).
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bioRxiv - Microbiology 2021Quote: ... coli DH5α (TaKaRa Bio, Inc., Shiga, Japan). Antibiotic susceptibilities of the recipient and transformants were assessed by microdilution as described above.
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains Stellar™ (Takara bio) and Rosetta™ 2 (Novagen ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (Takara Bio Co Ltd, Japan) beared the recombinant vectors were inoculated in LB medium containing 100 μg/ml carbenicillin ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) pLysS cells (Takara, China). The transformed BL21 cells were cultured in Luria broth at 37°C till 0.5 O.D growth and the cells were then induced with 0.1 mM IPTG (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... coli strain BL21/pG-Tf2 (Takara 9124), in the presence of Ampicillin (100 μM/mL ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2021: Escherichia coli DH5 alpha (Takara (Korea)) ...
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bioRxiv - Immunology 2020Quote: ... coli HST08 Premium Competent Cells (Takara Bio) and plated onto LB agar plates (Sigma–Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... coli JM109 competent cells (TaKaRa Bio Inc.) for amplification ...
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bioRxiv - Bioengineering 2022Quote: ... coli strain JM109 (Takara Biochemicals, Shiga, Japan) was used as the host for DNA manipulation and sensor strain construction ...
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bioRxiv - Microbiology 2022Quote: ... Stellar™ chemically competent Escherichia coli (Takara) were used to produce constructs for ectopic expression in mammalian cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... Stellar competent Escherichia coli cells (Takara Bio) were routinely grown in LB supplemented with kanamycin (Kan ...
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bioRxiv - Plant Biology 2024Quote: ... coli HST08 (Stellar competent cells, Takara Bio). Verified plasmids using Sanger sequencing were electroporated into A ...
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bioRxiv - Microbiology 2024Quote: ... Stellar chemically competent Escherichia coli (Takara Bio) was used to prepare plasmids for ectopic expression in mammalian cells.
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bioRxiv - Molecular Biology 2020Quote: ... we first produced lentivirus with Pup (E) plasmid Bio-Pup (E)-IRES-BFP within the Tet-On 3G inducible expression system (Clontech 631168), and infected OVCAR-5 cells for 48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... we first produced lentivirus with Pup(E) plasmid Biotin-Pup(E)-IRES-BFP within the Tet-On 3G inducible expression system (Clontech 631168), and infected Jurkat cells for 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... GST tags were cleaved by HRV-3C Protease (Takara), which released the recombinant proteins from the beads ...
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bioRxiv - Molecular Biology 2019Quote: ... an alkaline phosphatase (AP) tag (pSEAP; Clontech, CA, USA) was linked to the N-terminus of PAR1 cDNA (F2R ...
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bioRxiv - Cell Biology 2020Quote: ... or its derivatives with appropriate antibiotics resistant genes (ampicillin for Escherichia coli and neomycin or puromycin for mammalian cells) and a tag (SNAP-tag or HaloTag) using an In-Fusion HD Cloning Kit (639635, Takara Bio, Shiga, Japan). The E ...
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bioRxiv - Cell Biology 2020Quote: ... Rat anti-E-cadherin mAb (ECCD2; M108, Takara Bio), mouse anti-α-tubulin mAb (14-4502-82 ...
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bioRxiv - Neuroscience 2023Quote: ... SMARTer Stranded Total Sample prep kit-HI Mammalian (Takara, 634875) was used to generate libraries for RNA sequencing.
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bioRxiv - Immunology 2024Quote: ... His-tagged CD45RO was purified by TALON affinity resin (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA-seq analysis (150 bp PE reads, Novaseq 6000 platform, by Takara Bio; Table S2) as described above.
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bioRxiv - Molecular Biology 2021Quote: GroEL from Escherichia coli was purchased from TaKaRa Bio Inc ...
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bioRxiv - Microbiology 2019Quote: ... coli strains HST08 Premium (Takara Bio, Shiga, Japan), DH5α (Cosmo Bio ...
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bioRxiv - Biochemistry 2021Quote: ... coli Stellar™ cells (Clontech, see reagent Table). Transformation and bacterial screening were carried out using standard cloning methods ...
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bioRxiv - Molecular Biology 2022Quote: ... transformed into Escherichia coli DH5α (TaKaRa, Dalian, China) and finally sequenced at Shanghai Sangon Biotech Co ...
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bioRxiv - Molecular Biology 2022Quote: ... coli HST08 competent cells (TaKaRa Bio Inc., Japan) for cloning ...
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bioRxiv - Plant Biology 2023Quote: ... Stellar Escherichia coli HST08 cells (Takara Bio, Japan) and the Agrobacterium tumefaciens strain AGL1 were used for cloning and Z ...
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bioRxiv - Neuroscience 2022Quote: Escherichia coli BL21 (DE3) CodonPlus cells (Clontech, Inc.) were transformed with human α-synuclein-encoding plasmid (pET-21a(+) ...
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bioRxiv - Microbiology 2022Quote: ... coli strain BL21(DE3)-pGro7/GroEL (Takara Bio) was grown in 500 mL of ZYP medium [47] (supplemented with 100 μg/mL ampicillin ...
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bioRxiv - Plant Biology 2023Quote: ... coli competent cells were used (Takara, https://www.takarabio.com/). Finally ...
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bioRxiv - Genomics 2023Quote: ... coli using the Nucleobond XtraBAC kit (Takara 740436) and preps were stored at 4 °C.
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bioRxiv - Cell Biology 2023Quote: ... coli strain BL21-competent cells (TaKaRa Bio, TKR9126). Colonies were inoculated into LB broth for starter cultures incubated at 37 °C overnight ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli DH5α electrocompetent cells (Takara, Japan, product #9027), adding 2 uL of the product to 100 uL of electrocompetent cells ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli DH5α electrocompetent cells (Takara, Japan, product #9027) by adding 2 uL of (unpurified ...
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bioRxiv - Bioengineering 2024Quote: Escherichia coli strain HST08 (Takara Bio, Otsu, Japan) was used as the host for recombinant DNA manipulation ...
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bioRxiv - Molecular Biology 2019Quote: ... We inserted a green fluorescent C-terminal tag (EGFP; Clontech) analogous to a procedure used in a previous study [34] (for details ...