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Citations for Takara Bio :
301 - 350 of 1122 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... coli BL21(DE3) carrying the pGro7 plasmid (Takara, Shiga, Japan) for GroEL/ES chaperones co-expression48 ...
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bioRxiv - Molecular Biology 2024Quote: ... and then transformed in Escherichia coli HST08 competent cells (Takara). The sequence integrity of inserts carried by transformants were verified by Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Purification tags were removed by treating recombinant proteins with HRV3C protease (TaKaRa) and cOmplete™ His-tag Purification Resin (Roche) ...
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bioRxiv - Bioengineering 2019Quote: Sequencing libraries were prepared using the ThruPLEX Tag-seq Kit (Takara Bio). 10 μL of samples from NanoDeep assays (performed on SPR surfaces or cells ...
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bioRxiv - Biophysics 2022Quote: ... E-cadherin (24E10-Cell Signaling Technology; DECMA1-Sigma Aldrich, ECCD2, Takara Bio) (1:100)and paxillin (Abcam ...
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bioRxiv - Plant Biology 2021Quote: ... Culture mediums SD/-Leu/-Trp and SD/-Ade/-His/-Leu/-Trp (Clontech, USA) with or without X-α-gal were used to select the positive transformants.
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... and on a SD-Leu-Trp-Ade-His plate (ST0054, Takara Bio, USA) containing X-α-gal and supplemented with 0.2% Adenine ...
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bioRxiv - Systems Biology 2021Quote: ... selecting on SD -HIS agar plates (Takara Bio, 630411; Diagonal GmbH&CoKG, Y1751). The correct genomic context insertion of GFP of single colonies was confirmed by PCR using a combination of primers which also allowed the confirmation of the intron deletion strain ...
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bioRxiv - Cell Biology 2023Quote: Hexa-Histidine (6×His)-tagged bacterial expression constructs were created using pColdI (Takara) vector backbone ...
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bioRxiv - Microbiology 2021Quote: ... for expression with a C-terminal GFP tag or into pEGFP-C2 (Clontech) for expression with an N-terminal GFP tag and a codon-optimised synthetic gene (GeneArt ...
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bioRxiv - Cell Biology 2019Quote: FLAG-tag-hFAIM-S expression vector was constructed using pCMV-(DYKDDDDK)-C (Takara) (cloned into EcoRⅠ and KpnⅠ sites) ...
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bioRxiv - Plant Biology 2020Quote: ... Signal peptide– truncated cDNA fragments of AVRs were amplified by PCR by using primer set (Table S2) and inserted into EcoRI and BamHI sites of pGADT7 (prey) or pGBKT7 (bait) vectors (Clontech). sHMA cDNAs were synthesized from total RNAs of rice leaves (cultivar Sasanishiki ...
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bioRxiv - Genomics 2020Quote: ... A relative real-time quantitative PCR was then carried out for all the genes studied (Table S2) against SCR1 using SYBR Green Premix Ex Taq (Takara) in a Light Cycler 480 II (Roche).
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bioRxiv - Microbiology 2023Quote: ... Genes or gene fragments were PCR amplified with primers (S2 Table) to allow for In-Fusion gene cloning (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and used with the mixture of specially designed primers (SWITCH2 and RT_universal_primer, Supplementary Table S2) for cDNA synthesis (SMARTScribe Reverse Transcriptase, TaKaRa, Kusatsu, Japan).
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bioRxiv - Microbiology 2022Quote: ... 10 µg of Spike expressor and 2.5 µg of a green fluorescent protein (GFP) expressor (pIRES2-eGFP; Clontech) was transfected into 2 × 106 HEK 293T cells ...
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bioRxiv - Neuroscience 2021Quote: ... coli BL21 and purified on 5 mL Talon column (Clontech®) loaded with Cobalt ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli Rosetta (DE3) (DAD2 constructs) or BL21 (DE3) containing pGro7 (Takara) (ShHTL7 constructs ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli Stellar™ Competent Cells (TAKARA Bio Inc., San Jose, CA) was added to pre-chilled centrifuge tubes and chilled on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli strains StellarTM (Takara bio, San Jose, CA, United States) were grown at 37°C in LB broth (Difco ...
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Retrovirus-derived RTL9 plays an important role in innate antifungal immunity in the eutherian brainbioRxiv - Evolutionary Biology 2023Quote: ... 200 μM each of NTP and 800 nM of primers along with 0.625 units ExTaq HS (TaKaRa) were subjected to 32 cycles at 96°C for 15 s ...
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bioRxiv - Neuroscience 2022Quote: ... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
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bioRxiv - Plant Biology 2024Quote: ... were enriched from the lysate by immunoprecipitation using GFP-Trap (Lablead, GNM-25-1000) and were partially digested by micrococcal nuclease (2 × 10−5 U/μL, Takara, 2910A). The digested RNA was ligated to the 3′-RNA adaptor labeled by biotin ...
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bioRxiv - Cell Biology 2020Quote: ... S5C-E was generated by fusing human Rac1 cDNA into EGFP-C1 (Clontech) and kindly provided by Dr ...
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bioRxiv - Microbiology 2023Quote: ... RNase E was then purified using TALON® Metal Affinity Resin (Takara Bio). Protein purity was confirmed by SDS-polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... HIS-Ub-conjugated proteins were purified by cobalt chromatography (TALON metal affinity resin, Clontech), as described in the manual ...
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bioRxiv - Plant Biology 2021Quote: ... SD growth medium w/o Ade−His−Leu−Trp− (Himedia) and supplements media (Clontech) were used for the Y2H experiment.
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: ... and then grown on SD-Trp-Leu and SD-Trp-Leu-His plates (Clontech).
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bioRxiv - Synthetic Biology 2020Quote: ... The protein library was purified using a His-Tagged 96-well plate cartridge (Clontech) and buffer exchanged into 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... EPHA2- FN2 and antigen-A were purified using His 60 Ni Superflow resin (Takara), whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR reaction was performed with a Biorad CFX384TM Real Time System PCR machine and forward and reverse gene-specific primers (0.5 μM, Table S2) using the SYBR® Premix Ex Taq™ (Tli RNaseH Plus) from Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... The single isoform of DCR1 was amplified by RT-PCR from mRNA isolated from S2 cells and reverse transcribed using the Oligo(dT)-primed RNA to cDNA EcoDry premix (Takara Bio). The CDS for AGO1 and GW182 were PCR amplified from pAFW-Ago1 (Addgene #50553 ...
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bioRxiv - Microbiology 2022Quote: Amplified spike sequence was first gel-purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then further purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... with an N-terminal GFP tag using the In-Fusion HD Cloning kit (Takara).
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bioRxiv - Microbiology 2020Quote: ... The cDNA fused to a C-terminal HA tag was subcloned into pQXCIH (Clontech) in between the NotI and PacI sites to obtain the pQXCIH-TMPRRS2-HA vector.
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bioRxiv - Neuroscience 2020Quote: ... which was generating by replacing a V5 tag with an eGFP or DsRed (Clontech) tag from pcDNA3.1-nV5-DEST vector (Invitrogen) ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio), and the samples were sequenced on an Illumina HiSeq1500 system.
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bioRxiv - Microbiology 2020Quote: ... The ligated plasmid was then transformed to Escherichia coli HST08 (Takara Bio) and selected on chloramphenicol plates ...
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bioRxiv - Physiology 2020Quote: ... coli Stellar cells (Clontech/Takara Bio USA Inc., Mountain View, CA, USA). Cells were grown to an OD600 of about 0.5 and afterwards harvested ...
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bioRxiv - Microbiology 2021Quote: ... Genetic manipulations were carried out in Escherichia coli StellarTM competent cells (Clontech). Primers and plasmids used in this study are listed in Table S2 and S3 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli JM109 cell pellets by using NucleoBond Xtra Midi (TaKaRa Bio Inc.) and protocol for low copy plasmids ...
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bioRxiv - Microbiology 2022Quote: ... CoV-229E sequences were obtained as gBlocks from IDT and subcloned into these plasmids using InFusion cloning (Takara).
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bioRxiv - Plant Biology 2020Quote: ... The genetic regions containing cmaA and surrounding regions were amplified using PCR primer sets (S2 Table) that were designed based on the Pcal ES4326 registered sequence with Prime Star HS DNA polymerase (TaKaRa, Otsu, Japan), then dA was added to the 3’ end of the PCR product with 10 x A-attachment mix (TOYOBO ...
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bioRxiv - Molecular Biology 2020Quote: ... Both soluble and insoluble (cell pellet) fractions were purified via His-IDA nickel column (Clontech Laboratories ...
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bioRxiv - Genetics 2022Quote: ... Colonies were grown on media lacking histidine and leucine (DO Supplement -His/-Leu, Takara Bio) to select for the presence of both vectors ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) with 0.5-1ug of RNA and samples were sequenced on the NovaSeq (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... which was inserted into pAcGFP-His- MAP2C 11-308 by In-Fusion cloning kit (Takara). pAcGFP-His-MAP2C-Tau was chimera of MAP2C_M1-L311 and Tau0N4R_P193-L383 in the numbering Tau0N4R ...
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bioRxiv - Plant Biology 2023Quote: ... The yeast transformants were grown on nutrient-restricted (without Trp, Leu, His, Ade) mediums (Clontech) 3-5 days to assess interactions between various protein combinations.
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged TEV protease was removed by binding to Co2+-charged TALON resin (Clontech) and the flow-through concentrated using a 100 kDa molecular weight cut off concentrator (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... TagRFP-CENP-E 2111-C were cloned into the pLVX-IRES-Puro vector (Clontech) by PCR-based Gibson assembly method.