Labshake search
Citations for Takara Bio :
2501 - 2518 of 2518 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and BGH PCR products had 15 bp of overlapping sequence with adjacent inserts and backbone and were assembled with In-Fusion (Takara Bio, Mountain View, CA, USA) to create the final construct ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Genetics 2020Quote: ... nucleotides 610–792 of the map3 ORF were subjected to random mutagenesis by using the Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, Mountain View, CA, USA) in accordance with the manufacturer’s instructions for error-prone PCR ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR products were subsequently cloned into pGEMHE Xenopus laevis oocyte expression vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP) and the final sequence validated (Eurofin ...
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bioRxiv - Immunology 2023Quote: ... the library was prepared by synthesizing cDNA via reverse transcription and amplifying cDNA by long-distance PCR using the Takara SMARTer v4 kit (Takara Bio USA, San Jose, CA). 2×150bp sequencing was performed on an Illumina NovaSeq6000 instrument ...
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bioRxiv - Neuroscience 2023Quote: ... In-Fusion HD enzyme premix was added to the linearized PCR product and transformed into the Stellar™ Competent Cells (Takara; Bio Inc., Otsu, Japan). Integrity of plasmid sequences was verified through sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were sized in gels (as above) and bands of interest isolated using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio Inc., San Jose, CA). Sanger Direct Sequencing was performed at Azenta Life Sciences (South Plainfield ...
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bioRxiv - Evolutionary Biology 2024Quote: ... (300 ng DNA, 2.4 nM primers, 200 μM of each dNTP, 1x PCR buffer and 1.25 U Takara LA Taq polymerase in 25 μl reaction). Specificity of PCR products was confirmed on a 1% agarose gel and purified with AMPure PB beads.
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bioRxiv - Genetics 2024Quote: ... corresponding to predicted CNL and NL protein sequences were synthesized by General Biosystems (Durham, NC, United States) and subsequently used for PCR amplification with PrimeSTAR® Max DNA Polymerase assay (Takara Bio Inc., cat. # R045A) for cloning into pGBKT7 DNA-BD and pGADT7 AD vectors (Table S6) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we performed Rapid-Amplification-of-cDNA-Ends (RACE) PCR using transcript-specific primers and the SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc., Mountain View, CA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... We PCR amplified each region from 1 μL of the converted gDNA using TaKaRa EpiTaq HS for bisulfite-treated DNA (Takara Bio USA; Mountain View, CA, USA) per manufacturer’s instructions with primers designed using MethPrimer ...
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bioRxiv - Microbiology 2021Quote: ... Routine and high-fidelity PCR amplifications were performed using RedTaq (Denville Scientific, Metuchen, NJ, United States) and CloneAmp HiFi (Takara Bio USA Inc., Mountain View, CA), respectively ...
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bioRxiv - Biophysics 2023Quote: Plasmids used in this study (Supplementary Table S1) were assembled by Polymerase Chain Reactions (PCR) amplifying insert and vector DNA fragments followed by In-Fusion cloning (Takara Biosciences, In-Fusion HD Cloning Kit). Oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... a two-round PCR strategy was required to amplify the ORF using a high-fidelity PrimeStar ® DNA polymerase (Takara Europe, Saint-Germain-en-Laye, France). The first round of the nested PCR was carried out using the above cDNA as the template with primers designed in the 5’- and 3’-untranslated regions (UTR ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was introduced to measure the relative expression of RDase genes by using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (Takara Bio, Saint-Germain-en-Laye, Germany). Primers targeting RDase genes were designed using the NCBI primer design tool with the setting parameters ...
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bioRxiv - Molecular Biology 2024Quote: ... 1D libraries were prepared according to ONT protocol with 1D PCR Barcoding kit and full length non-directional sequencing was performed on PromethION instrument (using Clontech- SMART- Seq v4 Ultra Low Input kit). Basecalling was conducted using guppy version (v6.4.2 ...
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bioRxiv - Cell Biology 2020Quote: ... we designed primers with complementary overhangs (Supplementary Table S3) and used them to generate a “NEON insert” by PCR amplification (high-fidelity DNA polymerase, CloneAmp™, Takara Bio Europe, Saint-Germain-en-Laye, FR) of the mNeonGreen sequence (Allele Biotechnology and Pharmaceuticals Inc. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Library purification was achieved by pooling and purifying labelled amplicons using the NucleoSpin® Gel and PCR Clean-up kits (Takara Bio USA, Inc, Mountain View, CA USA) with three modifications to the NucleoSpin® User Manual ...