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Citations for Takara Bio :
2201 - 2250 of 2518 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Then PCR application using specific primer pairs (Supplemental Table 2) designed for H7N9 virus and Takara One-step RT-PCR kit (Takara, China) segment by segment ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification on the cleaned libraries was performed as follows: A reaction mix using LA Takara Hot Start kit (Takara Bio) was prepared according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: The expression of DGAT1s in the transgenic Arabidopsis lines was analyzed by quantitative RT–PCR (qPCR) on a Thermal Cycler Dice Read Real-Time System (Takara, Japan) with the SYBR Green Premix EX Taq GC™ (Takara ...
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bioRxiv - Biochemistry 2021Quote: ... Polymerase chain reaction (PCR) was performed using the primer set Fish-F1 and Fish-R229 and Tks Gflex DNA polymerase (Takara, Japan). The PCR product was directly sequenced at Macrogen Inc ...
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bioRxiv - Microbiology 2022Quote: ... the coding sequences of DivIVA and its T19A and T19E mutant alleles were PCR amplified using sequence-specific primers (Table 1) and cloned at BamHI-KpnI sites in pDsRed plasmid (Clontech Inc.) yielding pDsD4A and pDsD4AT19A ...
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bioRxiv - Physiology 2022Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
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bioRxiv - Genomics 2022Quote: ... colonies were picked into 96-well plates and a small proportion of cells from each colony were used for DNA extraction using Quick Extract solution (Epicenter) and direct PCR with Prime STAR® GXL DNA Polymerase (Clontech). PCR amplicons were sequenced by Sanger to verify the deletion (SFigure 17).
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bioRxiv - Plant Biology 2022Quote: We amplified the coding sequences of MpSETA and MpICE2 from cDNA derived from mRNA of Tak-1 thalli by PCR using PrimeSTAR Max DNA polymerase or PrimeSTAR GXL polymerase (Takara Bio). Also ...
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bioRxiv - Microbiology 2022Quote: ... the first round of PCR amplification was performed using Takara Ex Taq® Hot-Start Version (RR006A; TaKaRa Bio, Shiga, Japan). The amplification comprised 40 cycles of denaturation at 98 °C for 10 s ...
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bioRxiv - Genetics 2019Quote: ... The PCR amplicons were cloned into the NheI and SacII sites of the pEGFP-N1 expression vector (Clontech, Takara Bio, Japan). Mutant EGFP-FBN1 plasmids were generated by site-directed mutagenesis and their construction confirmed by direct Sanger dideoxy sequencing ...
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bioRxiv - Genetics 2019Quote: ... The PCR amplicons were cloned into the NheI and SacII sites of the pEGFP-N1 expression vector (Clontech, Takara Bio, Japan). Mutant EGFP-FBN1 plasmids were generated by site-directed mutagenesis and their construction confirmed by direct Sanger dideoxy sequencing ...
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Lateral organ diversification in plants mediated by the ALOG protein family of transcription factorsbioRxiv - Plant Biology 2019Quote: ... an MpTAW1 genomic fragment with a 10 kb upstream region and a 3 kb downstream region was amplified by PCR using Prime STAR GXL polymerase (TaKaRa, Japan) and subcloned into pENTR/D-TOPO (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2020Quote: ... Diluted cDNA was amplified using gene-specific primers (Table 1) and the TB Green real-time PCR master mix (TaKaRa, Japan). RT–PCR was used to verify the accuracy of the RNA-Seq data and detect the mRNA expression of the MYL2 gene in tissues and C2C12 cells at least in triplicate with specific paired primers.
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bioRxiv - Epidemiology 2019Quote: ... The PCR products of the three fragments were cloned into a pMD18-T easy vector (TaKaRa Biotechnology Co., Ltd., Dalian, China) for later sequencing (Syn-Biotechnology ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... The expression plasmids for circSPNS1 was constructed by subcloning the PCR-amplified fragments into FLAG-pcDNA3 plasmid using an In-Fusion HD cloning kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... murine Ptpn2 cDNA from HER-2-E0771 cells was reverse transcribed and amplified by PCR and cloned into the SmaI and EcoR1 restriction sites of Tre-3G plasmid (Clontech Laboratories) to generate the Tre-3G-Ptpn2 construct ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... were designed to amplify the internal region of 11β-h and 11β-hsd2 gene respectively by using Takara PCR Amplification Kit (Takara, Japan). To obtain the full-length cDNA sequences ...
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bioRxiv - Cell Biology 2019Quote: ... The PCR was performed with 30 cycles for amplification using the Ex Taq hot start version (Takara Bio Inc., Otsu, Japan) with 50 pM of primer sets ...
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bioRxiv - Biochemistry 2020Quote: ... The fragment encoding subdomains 4-6 (residues 434-782) was amplified by PCR and cloned between the XhoI and EcoRI sites of vector pCold (Takara Bio). The plasmids encoding the profilin isoforms ...
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bioRxiv - Synthetic Biology 2019Quote: ... Each fragment was individually amplified in a 20 μL PCR reaction using 0.8 μL PrimeSTAR GXL polymerase (Takara Bio Inc., #R050A), 0.8 μL of template DNA (10 ng μL−1 of plasmid template DNA isolated from E ...
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bioRxiv - Genomics 2020Quote: ... Probes were labelled by PCR in a volume of 25 µL consisting of 0.625 U Ex Taq polymerase (TaKaRa, Otsu, Japan), 1x Ex Taq buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... Aliquots of 1 μg of the treated RNA were used for cDNA synthesis with oligo dT using PrimeScript™ RT-PCR kit (Takara). cDNAs (0.4 μl ...
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bioRxiv - Biochemistry 2020Quote: ... The targeted gene and its flanking sequences were amplified by PCR with Synechocystis chromosome DNA as the template and cloned into pMD18–T vector (Catalog No: D101A, Takara, Japan). The physical map of the Synechocystis genome region containing sll0445–sll0447 or sll1334 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of the total RNA from the leaves was reverse-transcribed for 30 min at 42°C in a 20-μl reaction volume using a High Fidelity PrimeScriptTM RT-PCR kit (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... two to three amino acids in GFP-SP-BAP were replaced by alanine for each construct using the CloneAmp HiFi PCR Premix (Takara Bio) according to the manufacturer.
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bioRxiv - Molecular Biology 2021Quote: ... was inserted after amino acid 96 of N-terminally GFP-tagged SP followed by a stop codon using site-directed mutagenesis with the CloneAmp HiFi PCR Premix (Takara Bio). The BirA and ER-BirA constructs were obtained from Addgene (38 ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl sense and 1 μl anti-sense primers of 100 μM each were mixed with 2 μl 10X Taq polymerase PCR buffer (Takara, Japan) and 16 μl ultra-pure water to a final volume of 20 μl ...
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bioRxiv - Microbiology 2020Quote: ... QRT-PCR reaction in 20ul reactions containing 10ul TB Green Premix Ex Taq II(Tli RNaseH Plus)(TaKaRa, made in china), 3µl of cDNA and 0.4uM forward primers and 0.4uM reverse primers ...
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bioRxiv - Microbiology 2021Quote: ... leuC and leuD were measured by qRT-PCR using a SYBR Premix Ex Taq (perfect real time) kit (TaKaRa, Dalian, China). The recA gene was used as an internal control for B ...
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bioRxiv - Microbiology 2021Quote: ... The genome DNA was used as template for PCR amplification with the barcoded primers and Tks Gflex DNA Polymerase (Takara, USA). The hypervariable V3-V4 regions of bacterial 16S rRNA were amplified using universal primers 343F 5’-TACGGRAGGCAGCAG-3’ and 798R 5’-AGGGTATCTAATCCT-3’ (Nossa et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and pEU-bls-MCS were constructed based on each original vector by PCR and using the In-Fusion system (Takara Bio), or PCR and restriction enzymes ...
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bioRxiv - Microbiology 2021Quote: ... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ...
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bioRxiv - Genetics 2020Quote: ... The PE Sox1(+35)TFBS or Sox1(+35)TFBS+CGI inserts in Gata6-TAD or Gria1-TAD were amplified by PCR using EpiTaq polymerase (Takara Bio) and primers described in Extended Data Fig ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were performed in a 25μl-reaction mixture containing 12.5μl of 2 × Gflex PCR Buffer (Mg2+, dNTP plus) (TaKaRa Bio Inc., Shiga, Japan), 0.5μl of Tks
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bioRxiv - Immunology 2020Quote: ... the coding sequences of MRTFA and MRTFB were PCR amplified and subcloned into the pRetroX-Tight-Hygro vector (Takara Bio, 631034). Doxycycline inducible expression was achieved by pLVX-TetON-Advanced (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified and fused into a ClaI-digested plasmid with an In-Fusion HD cloning kit (TAKARA, Japan). Mutants for overexpressing HFB2 (TgOPB38530 ...
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bioRxiv - Developmental Biology 2020Quote: ... 2018) or new donor modules generated by cloning PCR fragments or custom gBlocks (IDT) into Eco53kI-digested vector pHSG298 (Takara Biosciences). For the Pvha-6::GFP::bbln-1::tbb-2 3’UTR and Pvha-6::GFP::BBLN (Hs)::tbb-2 3’UTR rescue constructs ...
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bioRxiv - Biochemistry 2021Quote: The proS gene was amplified from 50 ng population genomic DNA in a 25 μL PCR reaction using the high-fidelity polymerase PrimeSTAR Max DNA Polymerase (Takara Bio) and primers 134 and 138 (Supplementary Table 7) ...
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bioRxiv - Cell Biology 2020Quote: ... The sequences for a fusion protein consisting of circularly permutated GFP (cpGFP; developed in our lab (9)) and each Adhiron were amplified by PCR using PrimeSTAR MAX (TAKARA Bio) and inserted in modified pcDNA3 vector (71) ...
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bioRxiv - Developmental Biology 2021Quote: ... 350 ng RNA samples in 6 µL nuclease-free water) were generated using the Clontech SMARTer smRNA-Seq kit using 8 PCR cycles (Takara Bio). 30 µL of the PCR reaction was purified with AMPure XP beads (Beckman Coulter A63880 ...
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bioRxiv - Neuroscience 2021Quote: The desired ORF for Pdlim7 (Gene bank ID: AF345904.1) was amplified by PCR from Human Universal QUICK-Clone™ II (Takara 637260) using primers depicted in Table S2 and inserted into pEGFP-C1 plasmid through Gibson assembly.
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The resulting products were recombined into pDONR221 and transferred to the pTWGA vector to finally produced the desired plasmids ...
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bioRxiv - Genetics 2020Quote: ... of which 16 reactions (200 ng in each) were amplified for 29 cycles and purified using Macherey Nagel Nucleospin Gel and PCR Purification kit (Takara Bio). Sequencing libraries were made of the 4C PCR products using Thruplex DNA-seq kit (Takara Bio) ...
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bioRxiv - Genomics 2021Quote: ... reverse transcription and cDNA preamplification were performed in the C1 Fluidigm system using the SMARTer PCR cDNA Synthesis Kit (Clontech 634926) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... coli codon optimised pbp1 was PCR amplified using primers OPPF20018F/OPPF20018R and cloned into KpnI and SfoI cut pOPINJB by In-Fusion cloning (Takara Bio). The resulting construct ...
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bioRxiv - Microbiology 2020Quote: ... were used to transcribe poly (A)-tailed miRNAs to generate a cDNA library according to the PrimeScript™ miRNA RT-PCR Kit (Takara) instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The subcloning of FPs into respective vector(s) was done either by restriction enzyme (RE) digestion or by using In-Fusion PCR system for seamless cloning (Clontech, USA). Expression in HEK293 and mouse primary neuronal cells was facilitated by using vectors with either CMV or hSyn promoter ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed for gene expression using 2uL of 1:5 diluted cDNA with SYBR Green Realtime PCR Master Mix and Permix Ex Taq (Takara Bio), according to the manufacturer’s instructions ...