Labshake search
Citations for Takara Bio :
2151 - 2200 of 2518 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... three gene-specific reverse primers were designed and a nested PCR program was used according to the protocol of a genome walking kit (Takara, Japan). The sequences of the primers used for amplification are shown in Table S1 ...
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bioRxiv - Microbiology 2021Quote: ... and a linker sequence (5’-GGTAGCGGCAGCGGTAGC-3’) were added through three additional PCR reactions using PrimeStar GXL DNA polymerase (Takara Bio). PCR products were gel-purified in each step ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and was converted into cDNA prior to sequencing using the SMARTer technology (SMARTer Pico PCR cDNA Synthesis Kit, Takara, cat. # 634928). Sequencing libraries were prepared using the Illumina TruSeq protocol and sequenced on an Illumina MiSeq using 250 bp paired end reads ...
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bioRxiv - Evolutionary Biology 2021Quote: First amplified with primers 27F (5’-AGAGTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTT-3’) using EmeraldAmp GT PCR Master Mix (TAKARA BIO) to minimize the amplification of non-bacterial taxa under the following cycling conditions ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR fragment was prepared with a normal PCR protocol using KOD-Plus-Neo DNA polymerase (KOD-401, Toyobo, Japan) or ExTaq DNA Polymerase (RR001, Takara, Japan), both of which had 3’-5’ exonuclease activities.
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bioRxiv - Molecular Biology 2021Quote: ... The first stranded cDNA was next amplified to produce double stranded cDNA in 20 amplification cycles by long distance PCR using the Advantage 2 polymerase mix (Takara, Clontech) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: For the V1 protocol 20 µL of pooled and ExoI treated cDNA was PCR amplified with Advantage 2 Polymerase Mix (Clontech, #639206) in 50 µL total reaction volume using 1 µL of 10 µM LA_oligo (Microsynth ...
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bioRxiv - Immunology 2019Quote: ... Total RNA was then extracted from macrophages using the TRIzol method and converted into cDNA by PrimeScript™ RT-PCR Kit (Takara). The following genes were amplified ...
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bioRxiv - Genetics 2019Quote: ... qRT-PCR was performed in a 25 μl reaction volume with SYBR Premix Ex Taq II Tli RNaseH Plus (Takara Bio) on a Thermal Cycler Dice TP800 (Takara Bio) ...
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bioRxiv - Biophysics 2020Quote: ... coli MG1655 genome by colony PCR and cloned into pR6K-PAmCherry-lox71-cm-lox66 using the In-Fusion® HD cloning kit (Clontech). Mutations were introduced using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Biophysics 2019Quote: ... NG-Scarlet and NG-Cherry were created by deleting mRuby3 from NG-Ruby3 by inverse PCR and insertion of either mScarlet-I or mCherry using In-Fusion cloning (Takara Bio). NG-Stop was created using the same inverse PCR product as NG-Scarlet and NG-Cherry via blunt end ligation using NEBs KLD Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits) from Clontech (Mountain View, CA). Specific primers designed according to human ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was performed using Blend Taq polymerase (Toyobo, Tokyo, Japan) with a Thermal Cycler Dice TP600 (TaKaRa Bio, Otsu, Japan). The conditions were 5 min at 94°C ...
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bioRxiv - Zoology 2019Quote: ... Gene specific primers listed in supplementary information were used in quantitative RT-PCR reactions containing SYBR green master mix (Takara #RR820). The Ct values were used to calculate Fold Change against spike-in control lncRNA (see results for details ...
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bioRxiv - Genomics 2019Quote: ... The upstream and downstream homologous arms of each TF obtained by PCR amplification were introduced along with the pyrG marker into a pMD20 T-Vector (TaKaRa, Japan) by NEBuilder (New England Biolabs ...
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bioRxiv - Genetics 2019Quote: Generated cDNAs were diluted to 1/5 and were used as template in a real time quantitative PCR assay using SYBR®Premix Ex TaqTM II (Tli RNaseH Plus) (Takara). All qRT-PCR reactions were performed in duplicate using Bio-Rad CFX-96 Connect system with the following conditions ...
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bioRxiv - Biochemistry 2019Quote: ... the URAT1/SLC22A12 gene promoter region was amplified using KOD One PCR Master Mix (TOYOBO) or PrimeSTAR® Max DNA Polymerase (Takara) with the following primers ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...
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bioRxiv - Genetics 2021Quote: 0,1 ng of retrieved DNA in Cut&Run were used as template in a real time quantitative PCR assay using SYBR® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara). All qPCR reactions were performed in duplicates using Bio-Rad CFX-96 Connect system with the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... The Plus25neutral mutant was gener- ated via Gibson assembly using CloneAmpTM 2X HiFi PCR mix (Takara Bio USA, Mountain View, CA) for backbone amplification ...
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bioRxiv - Evolutionary Biology 2021Quote: The genomic region of approximately 300-600 kb centred on the position of the mutation was amplified by PCR with PrimeSTAR HS DNA Polymerase (TaKaRa Bio) and the corresponding primers (Table S4) ...
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bioRxiv - Cell Biology 2020Quote: ... and digested with NotI and MluI enzymes (restriction sites introduced via the PCR primer sequences) for introduction into similarly-digested pRetro-X-Tight (Takara Bio). The final insertion was confirmed by sequencing.
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio) with 25 cycles of amplification ...
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bioRxiv - Biochemistry 2020Quote: ... The PfNEDD8 PCR product was cloned into the StuI-HindIII digested pSMO plasmid using in-Fusion® HD Cloning Plus kit (Takara) and transformed into DH5α E ...
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bioRxiv - Cancer Biology 2020Quote: ... The synthesized oligo pool was amplified by PCR and cloned into LentiGuide-Puro backbone (#52963) by in-fusion assembly (Clontech #638909). The HOXA9P2A-mCherry reporter cell line was overexpressed with lentiviral Cas9 followed by infection of pooled sgRNA library at low M.O.I (~0.3) ...
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bioRxiv - Neuroscience 2021Quote: ... The cloning reaction for pSPE3::H2B-GCaMp6s was performed using linearised PCR products and the In-Fusion HD Cloning Kit (Takara Bio). H2B-GCaMP6s mRNA was produced and precipitated using the mMESSAGE mMACHINE T3 kit (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Cell Biology 2021Quote: ... was amplified from HEK293 cDNA by PCR using the primers 5’-TGTAAGCTTTTCGACACCACACCCCACTC-3’ and 5’-AGAGAATTCTCAGGAAAAGCTGTCATCGG-3’ and was inserted into pEGFP-C3 vector (TaKaRa Bio) using EcoRI and HindIII ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis was performed using TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio) on a Thermal Cycler Dice Real Time System TP800 (Takara Bio) ...
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bioRxiv - Plant Biology 2020Quote: ... The LightCycler 480II PCR system was programmed according to the instructions of the SYBR Premix Ex Taq II (Perfect Real Time) kit (TaKaRa, China) to conduct fluorescence-based quantitative PCR ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting cDNA templates were subject to qRT-PCR analyses using TB Green® Premix Ex Taq™ II (RR820Q, Takara) with a StepOneTM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2021Quote: ... An aliquot of 1 μg of DNAse treated RNA was used for cDNA synthesis using the PrimeScript™ RT-PCR Kit (Takara) in a final volume of 20 μL according to the manufacturer indications ...
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bioRxiv - Biochemistry 2021Quote: The final PCR products were inserted into the suicide vector pDM4 by In-Fusion ® HD cloning (Takara Bio USA, Inc). In short ...
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bioRxiv - Microbiology 2020Quote: ... qPCR experiments were performed in 72-well PCR plates using SYBR® Premix Ex TaqTMII-Tli RNaseH Plus (Takara, Dalian, China) in a 20-μL final reaction volume ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment of the TkR86C coding region was amplified from cDNA from the Canton-S wild type strain by PCR (PrimeSTAR GXL, TaKaRa Clontech) with primers that had NotI and XbaI sites at 5’ and 3’ ends ...
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bioRxiv - Cell Biology 2021Quote: ... The coding sequences of Arfs were amplified by PCR (primers listed in Suppl. Table S1) and inserted into the pQXCIP plasmid (Takara Bio) using the AgeI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time quantitative PCR reactions were set up in triplicate using SYBR Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa), Fast SYBR Green Master Mix (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: We quantified mRNA expression levels of IL-10 and chemokines identified in the RNA-Seq analysis using 2-step real-time RT-PCR (Thermal Cycler Dice Real Time System, Takara Bio). The ribosomal protein L13a (RPL13A ...
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bioRxiv - Cancer Biology 2022Quote: Full-length cDNAs were constructed using PCR amplification and subsequently inserted into the pLVX-IRES-neo vector (#632184; Clontech, CA, USA). The shRNA sequences were cloned into the pSIH1-puro vector (#26597 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs were PCR-amplified according to Takara’s SMART-Seq v4 protocol for 20 cycles using SeqAmp DNA Polymerase (Takara Bio, #638504) and a custom PCR primer ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA were also extracted from in vitro samples of cultured GC and reverse transcription reactions were performed using the SMART PCR cDNA synthesis technology (Takara Bio.) according to the manufacturer’s procedure and as previously published 35 ...
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bioRxiv - Molecular Biology 2022Quote: ... The complete EIAV gag-pol gene was PCR amplified from the molecular clone pCMV3-8 and inserted into the pEGFP-N1 vector (Clontech, USA) to construct the plasmid pGP ...
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bioRxiv - Molecular Biology 2022Quote: ... A 0.5 µl aliquot of the resulting genomic DNA solution was used as the template for genotyping purposes in 25 µl PCR reactions with Ex Taq DNA polymerase (TaKaRa, RR001). PCR products were subcloned with the TOPO TA cloning Kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was then performed using a Bio-Rad CFX-96 PCR detection system (USA) and a SYBR Green I mixture (Takara, Beijing). The MRSA housekeeping gene gyrB was used as an internal reference for normalization of the data.
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bioRxiv - Molecular Biology 2022Quote: ... Real-time RT-PCR analysis was performed using the Thermal Cycler Dice Real-Time System II (Takara Bio Inc., Shiga, Japan) to detect the mRNA expression of GPX4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Truncations of ENDOD1 and mutations of TP53 were obtained by fusing different PCR fragments using In-Fusion cloning kit (Clontech, 639650). Primers used in this study were listed in Supplementary Table S3.
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bioRxiv - Microbiology 2021Quote: The gfp gene was PCR amplified from pBAV1K-t5-gfp[32] and cloned into pUC18T-mini-Tn7T-Zeo by In-Fusion® Cloning (Takara). The pUC18T-mini-Tn7T-Zeo-GFP vector was conjugated to Acinetobacter as described[33] ...
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bioRxiv - Molecular Biology 2020Quote: Specific DNA sequences were PCR amplified using gene specific primers and cloned into vectors using PrimeSTAR HS DNA polymerase (#R010A, Takara-bio) and T4 DNA ligase (#M0202S ...
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bioRxiv - Molecular Biology 2020Quote: ... and hERG mutants were generated by PCR-mediated site-directed mutagenesis using an In-Fusion HD Cloning Kit (Takara, Otsu, Japan) as described previously (Kume et al. ...