Labshake search
Citations for Takara Bio :
201 - 250 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Purification Kit (Takara) was used for viral particle purification ...
-
bioRxiv - Developmental Biology 2021Quote: ... In-fusion HD kit and In-Fusion Snap Assembly kit (TaKaRa). pRSETa_mEos4b was a gift from Loren Looger (Addgene plasmid #51073 ...
-
bioRxiv - Cancer Biology 2023Quote: ... LipofectamineTM 2000 kit and reverse transcription kit were purchased from TaKaRa Company ...
-
bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed according to the Yeast Protocol Handbook (Clontech, catalog no. P3024). Briefly ...
-
bioRxiv - Cancer Biology 2021Quote: Yeast two-hybrid assay was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech/Takara, USA). Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara ...
-
bioRxiv - Cancer Biology 2021Quote: Yeast two-hybrid assay was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech/Takara, USA). Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara ...
-
bioRxiv - Bioengineering 2024Quote: ... SEAP production was analyzed by collecting medium and running a chemiluminescent assay on samples (Takara Bio) using a Tecan Infinite M1000 Pro plate reader.
-
bioRxiv - Molecular Biology 2023Quote: Y1H assays were performed using the Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The sequence of LhWRKY44 was inserted into the vector pGADT7 as prey ...
-
bioRxiv - Developmental Biology 2023Quote: Yeast two-hybrid assays were performed according to the Yeastmaker Yeast Transformation System 2 manual (Clontech). The yeast strain AH109 was co- transformed with an AD-fused and a BD-fused construct using the lithium acetate method ...
-
bioRxiv - Plant Biology 2024Quote: Y2H assays were conducted following the protocols outlined in the Matchmaker GAL4 Two Hybrid System (Clontech). Clone the full-length CDS of each target gene into pGADT7 and pGBKT7 vectors ...
-
bioRxiv - Immunology 2024Quote: ... The RNeasy kit and the cDNA Synthesis Kit (Takara, Cat#: 9767, RR047A) was used to extract total RNA and prepare cDNA following the manufacturer’s instructions ...
-
Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: LATaq Kit (RR002B, TaKaRa) was used in nested PCR ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3’RACE kit (Takara) was used to convert RNAs of the prostate tumors into cDNAs by a reverse transcriptase and oligo-dT adapter primer ...
-
bioRxiv - Systems Biology 2022Quote: ... The blocked membrane was incubated overnight at 4 ℃ in primary antibodies: mouse polyclonal anti-Cas9 (Takara Bio, cat. no. 632607), mouse monoclonal anti-β-actin (8H10D10 ...
-
bioRxiv - Cell Biology 2021Quote: ... The sequence encoding mCherry in frame with the polybasic sequence and CAAX motif of human K-Ras (GKKKKKKSKTKCVIM) for targeting to the plasma membrane was generated by amplification of mCherry using the pmCherry-N1 (Clontech) plasmid as template and the following primers ...
-
bioRxiv - Plant Biology 2024Quote: The yeast two-hybrid assays were carried out using a mating-based split ubiquitin system (mbSUS) for membrane protein interactions as described “mbSUS tests protocol” (Clontech) (Obrdlik et al. ...
-
bioRxiv - Neuroscience 2024Quote: Media was collected 48 h after transfection and clarified by syringe filtration through a 0.45 µm polyvinylidene difluoride membrane (Millex-HV) before concentrating the virus with a Lenti-X concentrator (Clontech 631231). The concentrated virus was resuspended in PBS and aliquoted into single-use tubes stored at −80 °C ...
-
bioRxiv - Neuroscience 2023Quote: Transgene constructs that simultaneously encode FingR targeting PSD95 and membrane markers of neuronal morphology were made using the In-Fusion HD Cloning System (Clontech). First ...
-
bioRxiv - Plant Biology 2023Quote: ... transferred membranes were hybridized overnight at 4 °C with the following primary antibodies: anti-GFP (Takara Bio Cat# 632381, RRID:AB_2313808) (1/5000) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell supernatant was collected and filtered through a 0.45 mM membrane and concentrated using Lenti-X Concentrator (Takara Bio, 631232).
-
bioRxiv - Plant Biology 2020Quote: ... β-galactosidase assay was performed according to the manufacturer’s protocol (Matchmaker Two-Hybrid System; Takara, https://www.takarabio.com).
-
bioRxiv - Biochemistry 2021Quote: ... an aliquot of the lysate was used to measure protein concentration by BCA protein assay (Takara Bio). The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer) ...
-
bioRxiv - Plant Biology 2021Quote: The Yeast two-hybrid assay was performed using the Matchmaker™ Gold Yeast Two-Hybrid System (Clontech). The full-length coding region of ZmBZR1 were ligated into pGBKT7 plasmid as bait and the full-length coding regions of ZmBEH1 was fused into pGADT7 vector as prey ...
-
bioRxiv - Plant Biology 2021Quote: ... The Y2H assays were performed following the manufacturer’s protocol using the Matchmaker GAL4 Two-Hybrid System (Clontech). Clones were grown on selective dropout medium (lacking either Trp and Leu or Trp ...
-
bioRxiv - Plant Biology 2021Quote: ... yeast one-hybrid assays were performed as the manual of Matchmaker Gold Yeast One-Hybrid System (Clontech). The coding sequence of BnaA03.WRKY28 was cloned into pGADT7 vector ...
-
bioRxiv - Microbiology 2020Quote: ... The purified virus was titrated with Lenti-X p24 Rapid Titer Assay (Takara Bio, Cat. No. 632200). The virus was stored at -80 °C for future use.
-
bioRxiv - Biochemistry 2021Quote: A portion of the viral supernatant collected for infectivity assays was concentrated using the Retro-X (Clontech) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: Yeast two-hybrid assays were performed in the yeast strain Y2Hgold with Matchmaker two-hybrid system (Clontech) according to manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2022Quote: The yeast two hybrid assay was performed as indicated in MATCHMAKER GAL4 two-hybrid system 3 (Clontech). The protein coding regions of genes used in this study were amplified from Guy11 cDNA with primer pairs listed in Table 1.1 ...
-
bioRxiv - Cell Biology 2022Quote: ... cereviseae strains Y2HGold and Y187 were used for Yeast Two Hybrid assay (Takara Bio, Mountain View, USA).
-
bioRxiv - Molecular Biology 2023Quote: ... Y2H assay was performed by the manufacturer’s protocol of the Matchmaker GAL4 Two-hybrid System (Clontech, USA). The prey and bait were cotransformed into Saccharomyces cerevisiae strain AH109 ...
-
bioRxiv - Plant Biology 2024Quote: ... Yeast transformation and growth assay were performed as described in the Yeast Protocols Handbook (BD Clontech, USA). In brief ...
-
bioRxiv - Plant Biology 2024Quote: ... Yeast transformation and liquid assay were conducted as described in the Yeast Protocols Handbook (BD Clontech, USA). The primers used for the Y1H assay are shown in Table S4.
-
bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assays were carried out using the Matchmaker Gold system (Takara™ Bio USA, Inc) with yeast strain AH109 ...
-
bioRxiv - Molecular Biology 2024Quote: The Matchmaker Gold Y1H system was used for yeast (S. cerevisiae) one-hybrid assays (Takara, https://www.takarabiomed.com.cn). The reporter strains were created by putting the MdKASI ...
-
bioRxiv - Developmental Biology 2024Quote: ... an aliquot of the lysate was used to measure protein concentration by BCA protein assay (Takara Bio). The lysate was mixed with OptiPhase HiSafe 3 (PerkinElmer) ...
-
Effects of Sanhuang plaster on expression of MyD88, TRAF6, MIP-1β and IL-23 in rats infected by MRSAbioRxiv - Molecular Biology 2022Quote: ... Real-time fluorescence quantitative PCR kit and reverse transcription kit(TaKaRa Co., Ltd.); MyD88 Anti-body TRAF6 Anti-body MIP-1β Anti-body and IL-23 Anti-body (GeneTexCo. ...
-
bioRxiv - Biophysics 2020Quote: ... using a cDNA synthesis kit (PrimeScript 1st strand cDNA Synthesis Kit, Takara Bio), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used for purification and titration ...
-
bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used ...
-
bioRxiv - Neuroscience 2024Quote: The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian kit (Takara Bio) with 300 ng RNA as input was used for library preparation according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... Membranes were stained with Ponceau S solution and then incubated with anti-GFP antibody (JL-8 Takara Bio Clontech, Lot #A8034133).
-
bioRxiv - Plant Biology 2021Quote: ... transferred to PVDF membranes by Western blotting and visualized with anti-GFP (JL-8 Takara Bio Clontech, Lot #A8034133, 1:5000), anti-α-tubulin (Invitrogen ...
-
bioRxiv - Plant Biology 2021Quote: ... transferred to PVDF membranes by Western blotting and visualized with anti-GFP (JL-8 Takara Bio Clontech, Lot #A8034133, 1:5000), anti-α-tubulin (Invitrogen ...
-
bioRxiv - Plant Biology 2021Quote: ... Membranes were stained with Ponceau S solution and then incubated with anti-GFP antibody (JL-8 Takara Bio Clontech, Lot #A8034133).
-
bioRxiv - Plant Biology 2024Quote: ... Samples were then run on a 4-12% Bis-Tris gel and transferred to a membrane for western blotting using α-GFP (Clontech) and α-HA (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2022Quote: ... were run onto 8% SDS- polyacrylamide gels that were transferred to nitrocellulose membranes which were reacted with α-His tag antibody (#631212, Clontech). Quantitation of band intensities was done with ImageLab software (BioRad) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Equal amounts of lysate were transferred to PVDF membranes that were probed with primary antibodies JL-8 anti-GFP (Clontech, USA) and β-actin ...
-
bioRxiv - Plant Biology 2022Quote: The Y2H assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, Mountain View, CA, USA). The full-length and truncated coding regions of WL1 were amplified and cloned into the yeast expression vector pGADT7 (Clontech ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using ONPG substrate following standard protocols (Clontech Laboratories). For two-hybrid experiments in meiotic conditions ...