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Citations for Takara Bio :
151 - 200 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The Y2H assays were performed in the Y2H Gold strain (PT4084-1, Takara 630489 ...
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bioRxiv - Plant Biology 2020Quote: Y1H assays were performed using a Matchmaker™ Gold Y1H System (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Yeast two-hybrid assay was performed using the Matchmaker Two-Hybrid System (Clontech) by following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cellular proliferation was measured with a WST–1 cell proliferation assay system (Takara). WST–1 reagent was added to each well and incubated for 2 hours at 37 °C ...
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bioRxiv - Genetics 2020Quote: Y2H assay was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... VLP were quantified by a LFA assay for HIV p24 (GoStix Plus, Takara). In this case the number of virions per volume is calculated by the approximate stoichiometry of p24 in VLP (∼2000/virion) ...
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bioRxiv - Bioengineering 2024Quote: ... VLP were quantified by a LFA assay for HIV p24 (GoStix Plus, Takara). In this case the number of virions per volume is calculated by the approximate stoichiometry of p24 in VLP (∼2000/virion) ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assays were performed using the MATCHMAKER GAL4 system (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was dried and pre-hybridized at 37 °C for 30 min in ExpressHyb (Clontech). Hybridization was performed in ExpressHyb containing RI-labeled probe at 37 °C overnight with rotation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were baked for 2 h at 80°C and prehybridized in ExpressHyb (Clontech, Mountain View) at 65°C for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... Hybridization of the membrane with the probe was performed using the ExpressHyb™ Hybridization solution (TaKaRa).
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bioRxiv - Cancer Biology 2022Quote: ... filtered with 0.22 μm membrane and concentrated by 50-fold with Lenti-X Concentrator (Takara, #631232). All cells were transduced by spinoculation at 2000 rpm for 1 hour at room temperature supplemented with 8 μg/mL polybrene ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: U937 cell lines stably expressing red fluorescent protein membrane were generated using pDsRed-Monomer-Mem (Takara Bio) cloned into pLB vector from Addgene (Addgene plasmid 11619 ...
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bioRxiv - Plant Biology 2020Quote: Yeast two hybrid assay was performed using Matchmaker Gold Yeast Two-Hybrid System (Clontech). The OsSalT gene was cloned in-frame into the EcoRI and BamHI RE sites of the yeast vector ...
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bioRxiv - Plant Biology 2021Quote: ChIP-Seq assays were performed on whole seedlings using anti-GFP antibody (Clontech 632592). Seedlings at 14 DAS (5 g ...
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bioRxiv - Plant Biology 2021Quote: ... Liquid β-galactosidase assays were performed as described in the Yeast Protocols Handbook (Clontech). A Y2HGold yeast strain containing a bait vector was mated with the Y187 strain containing the pGADT7 vector and selected on SD/-Trp/-Leu media ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR assay was performed using the TB Green Fast qPCR Mix (Takara). The ribosomal protein L21 gene (RPL21 ...
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bioRxiv - Plant Biology 2022Quote: Yeast one-hybrid assays were performed using the Matchmaker Yeast One-Hybrid System (Clontech). The DNA fragment 1,000 bp upstream of the start codon ATG of DDRM2 was cloned into pHis2.1 vector and SOG1 was cloned into the pGADT7 vector ...
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bioRxiv - Microbiology 2023Quote: RNA obtained from the RIP assay sample was purified using NucleoSpin RNA Plus (TaKaRa), followed by library construction and RNA-seq analysis ...
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bioRxiv - Immunology 2023Quote: ... As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories, USA) was transfected.
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined by bicinchoninic acid assay using bovine serum albumin (TaKaRa Bio) as the reference standard ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed with the MatchMaker GAL4 Two-Hybrid System 3 (Takara Bio). Saccharomyces cerevisiae strain AH109 was co-transformed with different pairs of pGADT7 and pGBKT7 harboring IREH1 or B1-Rafs ...
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bioRxiv - Plant Biology 2023Quote: ... the Y1H assays were performed following the manufacturer’s instruction (Clontech Laboratories, Inc., Kusatsu, Japan). The promoter sequence with and without mutation in ZDRE motif were artificially synthesized and cloned into the Kpn I-linearized pAbAi vector (Clontech Laboratories ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assays were based on the Matchmaker LexA two-hybrid system (Clontech). BPM genes were cloned into the pGlida vector ...
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bioRxiv - Plant Biology 2024Quote: ... The detailed procedures for Y2H assays were conducted based on the manufacturer’s protocol (Clontech).
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was incubated overnight at 4°C with an anti-GFP monoclonal primary (JL-8; Clontech 632381) at 1:5,000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... After incubation membrane was washed with 1X TBST buffer 3 times and detected with ECL reagent (TAKARA, Japan) using Versa Doc (BD Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated with a 1:2000 dilution of an anti-GFP antibody (Clontech 632381, clone JL8) for detection of Clover protein ...
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bioRxiv - Plant Biology 2020Quote: Y1H assay was performed using Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The nucleotide sequences of promoter region of selected key defense genes having potential RAV1 binding motifs were retrieved by using online tool (https://bioinformatics.psb.ugent.be/plaza/) ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast two hybrid assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The standard protocol given in the kit was followed for Y2H assay ...
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bioRxiv - Plant Biology 2021Quote: ... β‐galactosidase assay was performed according to the manufacturer’s protocol (Matchmaker Two‐Hybrid System; Takara Bio ...
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bioRxiv - Microbiology 2020Quote: The yeast two-hybrid assays were performed in strain Saccharomyces cerevisiae Y2HGold (Takara Bio USA). Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research) ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed by the Matchmarker GAL4-based two-hybrid System (Clontech). Constructs were co-transformed into the yeast strain AH109 by the lithium acetate method (Gietz & Woods ...
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bioRxiv - Genetics 2022Quote: ... and then yeast two-hybrid assays were performed in a Gal4-based system from Clontech in a diploid strain by mating as previously described (Rossignol et al ...
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bioRxiv - Plant Biology 2022Quote: Nuclear two-hybrid interaction assays were performed using the MATCHMAKER GAL4 two-hybrid system (Clontech). The CWLP coding region without signal peptide (SP ...
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bioRxiv - Plant Biology 2022Quote: ... The Y2H assays were carried out in the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The pairs of corresponding recombinant constructs fused with pGBKT7 BD and pGADT7 AD were co-transformed into the Y2H Gold yeast competent cells ...
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bioRxiv - Cell Biology 2024Quote: Following plasmids were used in this assay to generate stable cell lines: pCMV-HRasG12V (Clontech), Piggyback vectors ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative assays were performed in triplicate using SYBR Premix Ex TaqTM II (Takara #RR820A) in Bio-Rad iQ5 system ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast one-hybrid (Y1H) assays were performed according to the manufacturer’s instruction (Clontech, CA, USA). The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA isolation and real-time PCR assay Total RNA was extacted by RNAiso Plus (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-treated 1 hour prior to the assay with A/C Heterodimerizer (Takara) diluted at 1:200 in complete media concurrent with JF-Halo or -SNAP dye labeling ...
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bioRxiv - Microbiology 2023Quote: Y2H assays were performed according to Yeastmaker™ Yeast Transformation System 2 User Manual (Clontech). The coding sequences corresponding to HCPro1 ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast Two-Hybrid (Y2H) assays were carried out following the “Yeast Protocols Handbook” (Clontech Laboratories). Competent cells were transformed with 1 μg of DNA from each construct ...
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bioRxiv - Molecular Biology 2024Quote: ... yeast two-hybrid (Y2H) assays were performed using the Matchmaker Gold Y2H System (Clontech, USA). The full-length coding sequences of BnC07MYB3a ...
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bioRxiv - Immunology 2022Quote: ... Seq Kit (Takara), as per the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio), GAPDH was used as internal reference ...
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bioRxiv - Neuroscience 2024Quote: ... Purification Kit (Takara) was used for viral particle purification ...