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Citations for Takara Bio :
301 - 350 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The Universal GenomeWalker 2.0 kit (Clontech Laboratories Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). pIZ-Siwi-D670A and pIZ-BmAgo3-D697A mutants were generated by site-directed mutagenesis.
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). For Gtsf1L ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-PCR kit (Takara, Tokyo, Japan) was used to construct reverse transcription to collect cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... with the SYBR premeraseTaq kit (TAKARA). Amplified mRNA levels were normalized to 18s mRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT-qPCR using PowerUp SYBR-Green MasterMix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... SMARTer PCR cDNA synthesis kit (Clontech) was used according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of produced viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Molecular Biology 2024Quote: ... The TB Green kit (Takara Bio) was used for detecting miRNA expression levels ...
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bioRxiv - Molecular Biology 2024Quote: ... or PrimeSTAR Mutagenesis Basal Kit (TaKaRa). We used E ...
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bioRxiv - Cell Biology 2024Quote: ... A qRT-PCR kit (TaKaRa, Japan) was used to measure the expression levels of PCBP1 ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV titration kit (TaKaRa/Clontech). The purity of produced viruses was routinely checked by silver staining (Pierce ...
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bioRxiv - Genetics 2024Quote: Transcription Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and PrimeScriptTM RT reagent kit (Takara). RT-qPCR was performed using QuantiNova Probe PCR Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... using In-Fusion cloning kit (Takara). Truncated versions of FLAG::TurboID::SQSTM1 were generated by PCR using primers corresponding to the truncated SQSTM1 gene and amplification using Q5 high-fidelity (M0492 ...
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bioRxiv - Molecular Biology 2024Quote: ... retrotranscribed using PrimeScript Reagent Kit (Takara) and amplified by RT- qPCR using PowerUp SYBR-Green MasterMix (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... In-Fusion HD Cloning Kits (Takara) were used in Ligation Independent Cloning (LIC ...
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bioRxiv - Cancer Biology 2024Quote: An RNA extraction kit (Takara Kusatsu) was used for RNA extraction according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... In-Fusion HD cloning kit (Takara) was used to insert the fragments into the desired linearized vector featuring a GS linker-fluorescent tag fusion ...
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bioRxiv - Developmental Biology 2021Quote: Two-hybrid screening and assays were performed as described in the Matchmaker™ Pretransformed Libraries User Manual (Takara PT3183-1) using a Drosophila Mate & Plate™ Library - Universal Drosophila (Normalized A+ RNA isolated from embryo (~20 hr) ...
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bioRxiv - Molecular Biology 2021Quote: Nested translocation PCR assays was performed from genomic DNA as previously described (29) using either LA Taq DNA polymerase (Takara) or Flex HS polymerase (NEB) ...
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bioRxiv - Plant Biology 2020Quote: ... Plasmids for the PARN activity assay were constructed by inserting the coding sequence of RRD1 or human PARN (hPARN) into the pHAT vector (Clontech). The hPARN sequence was derived from the GNP Human cDNA clone IRAK071M01 (RIKEN BioResource Research Center) ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were performed as described (Cesari et al., 2013) using the Matchmaker Gold Yeast Two-hybrid system (Clontech).
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bioRxiv - Immunology 2022Quote: Lytic cell death was determined by measuring LDH release from cell-free supernatants using a colorimetric assay (Promega or Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Virus production was quantified by determining the amount of the Gag p24 protein using an enzyme-linked immunosorbent (ELISA) assay (Innogenetics or TaKaRa). For production of HIV-1 Env-pseudotyped viruses in Jurkat cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Confirmatory “1-to-1” pairwise assays for selected interactants were performed with the MatchMaker Two-Hybrid System 3 (Clontech Inc.)
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bioRxiv - Genomics 2020Quote: Yeast one-hybridization assay was performed using the Matchmaker® Gold Yeast One-Hybrid System (Clontech, Palo Alto, CA, USA). The promoter sequence (upstream 2kb from the start codon ...
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bioRxiv - Molecular Biology 2023Quote: ... but was tested in a directed two-hybrid assay in which cDNAs encoding ATI1 and ATI2 were cloned into the prey vector pGADT7 (Clontech) and co-transformed into PJ69-4A with the NcGNAGO1 bait plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... two-hybrid assay was conducted according to the manufacturer’s instructions for the Match-maker Gold Yeast Two-Hybrid System (Clontech, USA). To generate the bait construct BD-GhCPK28 ...
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bioRxiv - Microbiology 2023Quote: ... culture supernatants were collected from each well to measure lactate dehydrogenase (LDH) release by using an LDH cytotoxicity assay (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... culture supernatants were collected from each well to measure lactate dehydrogenase (LDH) release by using an LDH cytotoxicity assay (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: A yeast two-hybrid assay was conducted following the Clontech Yeast Two-Hybrid System protocol (Clontech, Mountain View, CA, USA). BGI1 was used as bait for screening a cDNA library constructed from Fielder (Li et al. ...
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bioRxiv - Microbiology 2024Quote: ... All protein-protein interactions were validated by conducting α-galactosidase activity assays using the X-α-Gal reagent (Clontech, USA).
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bioRxiv - Neuroscience 2020Quote: ... and reverse transcribed using complementary DNA (cDNA) synthesis kit (PrimeScript 1st strand cDNA Synthesis Kit, Takara Bio). The synthesized cDNA was subjected to qPCR using KAPA SYBR (KAPABiosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Viral titer was determined using a qPCR Lentivirus Titration Kit (Lenti-X, qRT-PCR Titration Kit, Takara). For smaller scale virus preparation ...
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bioRxiv - Neuroscience 2020Quote: ... in a 100 mm dish using a calcium phosphate transfection kit (CalPhos Mammalian Transfection kit, Clontech, 631312). Two days post-transfection ...
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bioRxiv - Synthetic Biology 2022Quote: ... The kit used for qPCR experiments was PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan), HiScriptII 1st Strand cDNA Synthesis Kit (Vazyme Biotech Co. ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using the ThruPLEX DNA-Seq Kit and DNA Unique Dual Index Kit (Takara) according to the manufacturer’s manual ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant constructs was transformed into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: 0,1 ng of retrieved DNA in Cut&Run were used as template in a real time quantitative PCR assay using SYBR® Premix Ex TaqTM II (Tli RNaseH Plus) (Takara). All qPCR reactions were performed in duplicates using Bio-Rad CFX-96 Connect system with the following conditions ...
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bioRxiv - Plant Biology 2020Quote: ... For Yeast-Two-Hybrid assays HvRIPb and HvRIPb truncations were subcloned from the pGEM-T easy vector into pGADT7 and pGBKT7 plasmids (Clontech Laboratories) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR assay was performed using 1/20 diluted cDNA as templates in the reactions containing SYBR® Premix Ex TaqTM II (TaKaRa). The qRT-PCR assay was conducted in triplicate in an ABI 7500 Fast Real-Time PCR System ...
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 100 ng DNA with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... followed by selection of transformants and testing of pair-wise interactions by growth complementation assays on nutrient selection media as described in the Matchmaker™ GAL4 Two-Hybrid System 3 manual (Clontech). To compensate for auto-activation of some constructs ...
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bioRxiv - Physiology 2024Quote: ... qRT-PCR assays were conducted in a 20 μL reaction volume employing TB Green Premix Ex TaqTM (Tli RNaseH Plus) (Takara, Japan). GAPDH served as the internal control ...
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bioRxiv - Genomics 2022Quote: ... Paris) were prepared following the kit manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit from Takara). Samples were sequenced on a NextSeq 500 Illumina device with MidOutPut cartridge to generate a total of 2 × 87 M reads of 75 bp ...