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Citations for Takara Bio :
101 - 150 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... membranes were incubated with mouse anti-GFP (Clontech #632380), rabbit anti-DsRed (Takara #632496) ...
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bioRxiv - Immunology 2023Quote: ... which was measured using a real-time PCR assay with a SARS-CoV-2 direct detection RT‒qPCR kit (Takara Bio, Siga, Japan). The IC50 was calculated by IC50 Calculator (https://www.aatbio.com/tools/ic50-calculator ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then blocked in ExpressHyb hybridization solution (Clontech) for 1 hour.
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bioRxiv - Microbiology 2024Quote: ... The membrane was probed with either anti-Flag (Clontech, Japan) or anti-Myc (Santa Cruz Biotechnology ...
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bioRxiv - Physiology 2024Quote: ... The concentration of AOPP was normalized to total protein content determined by bicinchoninic acid assay (BCA) assay (Takara Bio Inc.).
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bioRxiv - Cell Biology 2024Quote: ... Cell viability was measured after 72h of treatment using the WST-1 assay (WST-1 Cell Proliferation Assay System, ref MK400, TaKaRa) according to the supplier’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was determined by Bradford assay (TAKARA). Nickel affinity purification of 10His-SUMO1T95R conjugates was carried out with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... For cell death measurement by LDH assay (Takara), culture supernatants were clarified with a 5 minute spin at 400×g ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated in anti-GFP (Takara Bio, 1:5000 dilution) antibodies followed by a 2 h incubation in secondary goat anti-mouse-HRP (1:10,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with anti-GFP antibody (JL-8; Clontech) at a dilution of 1:5,000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The membrane was incubated with an α-GFP primary antibody (Takara Bio cat# 632380 ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were stripped using Western BLoT Stripping Buffer (Takara Bio, #T7135A) and re-probed with the desired antibody ...
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bioRxiv - Immunology 2020Quote: ... The protein concentration was determined by Bradford assay (Takara), and the cell lysate was mixed with 4x Laemmli loading buffer containing β mercapto-ethanol ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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bioRxiv - Microbiology 2020Quote: ... The membrane was then pre-hybridized in 10 mL ExpressHyb (Clontech, NC9747391) at 44°C in a rotating oven for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was incubated with Western BLoT Chemiluminescence HRP Substrate (TaKaRa Bio), and membrane image was captured using a chemiluminescent imaging system LuminoGraph I (ATTO ...
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bioRxiv - Microbiology 2024Quote: ... the solubilized membrane fraction gets incubated with TALON SuperFlow resin (Takara 635502) for 1 hours at 4°C and was later washed using buffer B supplemented with 20 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then pre- hybridized in 10 mL ExpressHyb (Clontech, NC9747391) at 44°C in a rotating oven for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane was then incubated with 1:1000 mouse anti-His6 antibody (Clontech) for 15 mins ...
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bioRxiv - Cancer Biology 2020Quote: ... WST-1 assays were performed as per manufacturer’s instructions (Takara) (18).
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid assays were performed using strain AH109 (Takara). Vectors pGADT7 and pGBKT7 were sequentially transformed into this strain ...
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bioRxiv - Plant Biology 2022Quote: Y2H assays were performed according to the manufacturer’s protocol (Clontech). For Y2H screening ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were pre-hybridised at 50 °C in ExpressHyb Hybridization Solution (Clontech, USA) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were washed with PBS with Tween® 20 (TAKARA BIO INC.) three times and incubated with secondary antibodies for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... After cross-linking membranes were pre-hybridised in ExpressHyb Hybridization Solution (Clontech, USA) at 40 °C for 1 h ...
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bioRxiv - Plant Biology 2022Quote: ... the membranes were stripped for 15 minutes with Western BLoT Stripping Buffer (Takara) following the manufacturer’s instructions and reprobed with Ubiquitin11 (Agrisera AS08307 ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were then incubated with either primary monoclonal antibodies anti-mCherry (Clontech #632543 ...
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bioRxiv - Cell Biology 2021Quote: The membrane was then incubated with Western BLoT Hyper HRP Substrate (Takara: T7103B) for chemiluminescence detection ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were UV cross-linked and pre-hybridized with ExpressHyb Hybridization Solution (Clontech) for 1 h at 50°C ...
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bioRxiv - Bioengineering 2024Quote: ... lentivirus targeting mCherry to the cell membrane (Takara, 0026VCT, rLV.EF1.mCherry-Mem-9) was used according to manufactures recommendation ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinked membranes were prehybridized for ∼1 hour at 55C in hybridization solution (Takara). DNA oligos (Table S1 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins blotted on membranes were visualized using TaKaRa CBB Protein Safe Stain (TaKaRa) and chemiluminescence and CBB staining were visualized using ImageQuant LAS 500 mini (Cytiva).
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bioRxiv - Synthetic Biology 2021Quote: ... Pseudotyped viruses were quantified with a p24 assay (Takara Bio #632200) per manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Y2H assay was performed according to the Yeast Protocols Handbook (Clontech) using the Y2H Gold yeast reporter strain (Clontech) ...
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bioRxiv - Plant Biology 2023Quote: Y1H assay was conducted according to protocol (Clontech, cat. No. 630491). Briefly ...
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bioRxiv - Plant Biology 2023Quote: Y2H assay was performed according to protocol (Clontech, cat. No. 630489). Since BcICE1 self-activates ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast two-hybrid assays were performed using yeast strain AH109 (Clontech) following standard procedures (Yeast Protocols Handbook PT3024-1 ...
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bioRxiv - Genomics 2024Quote: ... Cell lysates were quantified using the bicinchoninic acid assay (BCA, Takara). For each sample ...
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bioRxiv - Microbiology 2021Quote: ... Dried membranes were pre-hybridized in 8 ml of pre-warmed ExpressHyb buffer (Clontech) for 1 h at 55°C before adding filtered 32P-labeled probes and incubating at 38.5°C overnight (Biometra OV5 ...
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bioRxiv - Biophysics 2024Quote: ... MelBSt purification from membranes by cobalt-affinity chromatography (Talon Superflow Metal Affinity Resin, Takara) after extraction by 1.5% UDM ...
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bioRxiv - Plant Biology 2021Quote: Cell viability assays were performed by using either Y2HGold yeast strains (Clontech) containing a pBridge bait vector or diploid yeast strains generated by mating a Y2HGold strain containing a bait vector with a Y187 strain (Clontech ...
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bioRxiv - Plant Biology 2021Quote: Y2H assays were performed using the Matchmaker™ Two-Hybrid System (Clontech). Bait (pGBKT7 ...
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bioRxiv - Plant Biology 2021Quote: Y2H assays were performed using the Matchmaker GAL4 two-hybrid system (Clontech). The full-length and/or partial (RING ...
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bioRxiv - Immunology 2020Quote: ... The PCR assays were run using LA Taq polymerase (Takara Bio, TAKRR002) with the following thermocycling conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µL of premix WST-1 cell proliferation assay (Takara Bio, Inc.) was added to each well and the plate was incubated for an additional 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The tetrazolium salts from Premix WST-1 Cell Proliferation Assay System (TAKARA) are cleaved by mitochondrial dehydrogenase in viable cells to produce formazan dye ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast two hybrid assays were carried out using Gal4 based system (Clontech) by introducing plasmids harboring gene of interest in yeast strains AH109 and Y187 ...
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bioRxiv - Developmental Biology 2020Quote: ... Northern blot analysis was performed on a membrane containing RNA from adult mouse tissues (Clontech). As probe ...
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bioRxiv - Plant Biology 2020Quote: ... Immunodetection was performed by incubating the membranes in the Western BLoT Quant HRP Substrate (Takara) and recording the chemiluminescence by LuminoGraphI(ATTO).
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were probed with commercial mouse monoclonal antibodies: anti-GFP (Clontech Living Colors JL-8), anti-mCherry (Abcam 1C51 ...