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Citations for Takara Bio :
51 - 100 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and the protein concentration was measured by BCA protein assay kit (TaKaRa, Dalian, China, cat#T9300A) as previous described(Ma et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was found to be 0.9 µg/µl using BCA Protein Assay Kit (TaKaRa).
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bioRxiv - Physiology 2022Quote: ... Obtained luminescence was normalized to total protein concentration measured by BCA protein assay kit (Takara Bio).
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bioRxiv - Microbiology 2024Quote: Protein concentrations were determined using the BCA method (TaKaRa BCA Protein Assay Kit; Takara, Shiga, Japan) after 1% SDS was added to solubilize the samples ...
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bioRxiv - Plant Biology 2024Quote: ... The Y1H assay was conducted using a Y1H Library Screening Kit (Clontech, Mountain View, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: The enzymatic activity of HPSE was measured using a Heparan Degrading Enzyme Assay Kit (Takara, MK412) as per the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... and a DNA ladder assay was performed using an ApopLadder Ex kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: An RNA blot membrane (Clontech) was hybridized with the 32P-labeled full coding sequence of rMap7D2 ...
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bioRxiv - Microbiology 2020Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Monocytes were infected immediately after purification by adding HIV-1BaL virus to the culture at MOI between 3 and 5 based on the p24 titer ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations of cell lysates were measured using a bicinchoninic acid protein assay kit (Takara Bio Inc.). Cell lysate containing the same amount of protein was mixed with 10 volumes of methanol and then centrifuged at 20,000 × g for 30 min to precipitate proteins ...
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bioRxiv - Epidemiology 2021Quote: ... and SYBR Premix Ex Taq Kit for real-time PCR assay were purchased from Takara (Dalian, China). Monoclonal CD3-FITC/APC ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a T7 endonuclease I (T7EI) assay using the Guide-it Mutation Detection Kit (Takara Bio) following the standard protocol for mismatch detection.
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bioRxiv - Microbiology 2022Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Infections of fully differentiated MDM were performed after adherent growth in the presence of M-CSF for seven days ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined via the Bradford method using the TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Physiology 2022Quote: ... Media lactate dehydrogenase (LDH) was assayed by enzymatic colorimetric assay (LDH Cytotoxicity Detection Kit, Takara Bio, cat# MK401).
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bioRxiv - Biochemistry 2024Quote: A pull-down assay was performed using a Capturem His-Tagged Purification Miniprep Kit (#635710, Takara, Shiga, Japan). 50 µg of BSA was added to the column for blocking ...
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bioRxiv - Plant Biology 2024Quote: ... This suspension was used to determine total protein concentrations using Takara BCA Protein Assay Kits (TaKaRa, Kusatsu, Japan).
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bioRxiv - Immunology 2021Quote: ... was used to for two-step qPCR assay after cDNA synthesis using PrimeScript® reverse transcriptase kit (Takara, Japan). Gene expression was normalized to the expression level of β-actin ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... CDC-USA assay targeting gene N (IDT # 10006713) and One Step PrimeScript™ III RT-PCR Kit (TaKaRa #RR600A). Serial dilutions of reference material were prepared ranging from 1 to ~10M genome equivalents per reaction ...
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bioRxiv - Neuroscience 2023Quote: TUNEL assays were performed to detect apoptotic cell death using Clonetech ApoAlert DNA Fragmentation kit (Takara, Kusatsu, Shiga, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and the protein concentration of the supernatants was determined using the BCA Protein Assay Kit (Takara Bio, Inc., Japan). Ten μg of proteins were treated with 10 μM tetramethylrhodamine (TAMRA)-alkyne (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and the protein concentration of the supernatants was determined using the BCA Protein Assay Kit (Takara Bio, Inc., Japan).
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and detected by RT-qPCR assays with a One-Step PrimeScript RT-PCR kit (Takara, Japan) using SARS-CoV-2-specific primers on an Applied Biosystems 7500 Real-time PCR System.
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Final cDNA samples were used then used for quantitative real-time PCR assay by SYBR Green PCR Kit (RR820A, Takara). Quantitative primers were designed based on the gene sequences by Sangon Biotech ...
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bioRxiv - Synthetic Biology 2023Quote: ... SEAP activity in supernatant (25 μL) was measured using the Great EscAPe SEAP Chemiluminescence Assay kit 2.0 (Clontech, Fremonet, CA). The GLuc activity in supernatant (20 μL ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Immunology 2022Quote: ... RNA concentrations were determined using the Qubit high-sensitivity RNA assay and small RNA libraries were created using the Clontech SMARTer smRNA-Seq preparation kit according to manufacturer instructions (Takara). Small RNA inserts were further enriched using AMPure bead selection (Beckman Coulter ...
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bioRxiv - Biophysics 2024Quote: ... The concentration of purified proteins was determined by the Bradford method using TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Biochemistry 2024Quote: ... Supernatant containing viral particles was filtered through a 0.45-μm filter and the lentivirus titer determined by enzyme-linked immunosorbent assay (ELISA) with the Lenti-X p24 Rapid Titer (Single Wash) Kit (Takara). To generate cell lines expressing MDA5 variants ...
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bioRxiv - Physiology 2023Quote: The WST-1 assay (Takara) was performed on HepG2 cells and Huh7 cells to assess the cellular cytotoxicity of Tecomella undulata ...
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bioRxiv - Plant Biology 2020Quote: ... Yeast two-hybrid assay and one-hybrid assay were performed following the manufacturer’s instructions (Clontech).
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bioRxiv - Plant Biology 2020Quote: ... The yeast one-hybrid (Y1H) assay was conducted using the Matchmaker™ Gold Yeast One-Hybrid Library Screening System kit (Cat. no. 630491, Clontech).
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL assay was performed to detect apoptotic cell death using the in situ Apoptosis Detection Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The Y2H assay was performed following the manual provided in the kit “Matchmaker Gold Yeast Two-Hybrid” (Clontech, Mountain View, USA). The list of VirE2 interacting clones has been given in Fig.S1.
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bioRxiv - Molecular Biology 2024Quote: ... Apoptotic cells were quantified via the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay using the In situ Apoptosis Detection kit (Takara Bio). Visualization of apoptotic cells was performed using the Evos FL Cell Imaging System (Life Technologies).
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bioRxiv - Microbiology 2022Quote: ... Membranes were hybridized in ExpressHyb Hybridization Buffer (ClonTech) to random-primed 32P-labeled DNA probes (RadPrime DNA labelling system ...
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bioRxiv - Biophysics 2020Quote: ... Solubilized membranes were incubated with Talon resin (Clontech) and then with 100 μM TCEP reducing agent to obtain A2AR-bound Talon resin suspended in 50 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were hybridized in ExpressHyb Hybridization Buffer (ClonTech) to random-primed 32P-labeled DNA probes (RadPrime DNA labelling system ...
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bioRxiv - Immunology 2024Quote: ... The membrane was developed with ECL reagent (Takara). Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3 ...
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bioRxiv - Immunology 2021Quote: ... The transcripts of the DEMs and DEPs were analyzed and validated by qRT-PCR assays using the Mir-X miRNA qRT-PCR SYBR® Kit (TaKaRa, Japan) and the PrimeScriptTM RT reagent Kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the primers of the U6 snRNA used in the qRT-PCR assays were provided in the Mir-X miRNA qRT-PCR SYBR® Kit (Takara, Japan). The qRT-PCR data were analyzed by the 2−ΔΔCt method as described in Livak & Schmittgen (2001 ...
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bioRxiv - Microbiology 2023Quote: The 5’ and 3’ termini of the sRNA OueS were determined by the RACE assay (SMARTer RACE 5’/3’ kit, Takara Bio USA), adapted for non-poly-A-tailed RNA ...
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bioRxiv - Microbiology 2023Quote: ... levels were measured by a qRT-PCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A). The PCR protocol was 42°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... vRNA copy numbers of each virus stock were measured via RT-qPCR assay with the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A) as described previously [12] ...
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bioRxiv - Microbiology 2024Quote: The 5’ and 3’ termini of the sRNA OueS were determined by the RACE assay (SMARTer RACE 5’/3’ kit, Takara Bio USA), adapted for non-poly-A-tailed RNA ...
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bioRxiv - Synthetic Biology 2024Quote: The MetLuc reporter assay was performed using Ready-To-Glow™ Secreted Luciferase Reporter Assay (Clontech, 631728) per the manufacturer’s instructions in a white 96-well (flat-bottom ...
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bioRxiv - Immunology 2021Quote: ... The membranes were subsequently stripped (Takara BioInc, Kusatsu, Japan), blocked ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated with 32P-radiolabeled ura4 probe (TaKaRa BcaBESTTM Labeling Kit ...