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Citations for Takara Bio :
2401 - 2450 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... PCR was performed using TaKaRa Ex Taq Hot Start Version (TaKaRa) with primers designed at 3’UTR of wallaby ZDBF2 according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... gDNA was amplified using CloneAmp™ HiFi PCR Premix (Takara 639298) and dmd Sanger sequencing primers ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... Predicted enhancer elements were PCR-amplified from mouse genomic DNA (Clontech) and cloned into a Hsp68-LacZ expression vector for random integration114 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative real-time PCR using SYBR Green based method (DSS TAKARA) was employed to estimate relative transcript levels of mRNAs with GAPDH as housekeeping control gene for internal normalization ...
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bioRxiv - Plant Biology 2024Quote: ... Genotyping PCR was performed using Prime-STAR GXL DNA Polymerase (Takara) in the presence of 5% DMSO (Kwantes and Wichard 2022 ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were digested using Dpn I (Takara, cat. no. 1235A) for 2 hours at 37 °C and transformed into Trans5 α bacterial chemically competent cells (TransGen Biotech ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCRs were performed with PrimeSTAR Max DNA Polymerase (Takara Bio) using the described primer sets (Table S1).
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bioRxiv - Cancer Biology 2024Quote: ... using the SuperPlex Premix PCR master mix (Takara, Otsu, Shiga, Japan). PCR1 ...
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bioRxiv - Neuroscience 2024Quote: ... with the TB Green Premix Ex Taq PCR master mix (Takara). For each sample ...
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR was performed with SYBR Premix Ex TaqTM (TaKaRa) on a LightCycler 480 II (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... PCRs were performed using the PrimeSTAR Max DNA Polymerase (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... SISPA PCR amplification was conducted with TaKaRa Taq DNA Polymerase (Takara) using the primer FR20RV (5’-GCCGGAGCTCTGCAGATATC-3’ ...
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bioRxiv - Biochemistry 2024Quote: ... PCR was performed with PrimeSTAR® GXL DNA Polymerase (Takara, R050A), PrimeSTAR GXL Buffer (Takara ...
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bioRxiv - Developmental Biology 2020Quote: ... Expression levels of the stag paralogues (primer sequences in Supplementary Table S3) and haematopoietic markers were measured using SYBR Premix Ex Taq II (Takara Bio Inc., Kusatsu, Japan) on a Roche LightCycler400 ...
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bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (0.4 U) and 1.92 μM of the 3’ oligo dT terminating primer: SMART-Seq® ICELL8® CDS (Takara Bio USA, CA, USA).
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bioRxiv - Plant Biology 2019Quote: ... strategy and internal primers designed from the genomic contigs (Table S1) following the kit supplier’s recommendations (Takara Bio Europe/Clontech, Saint Germain-en-Laye, France). The 3’ ends were amplified using forward internal and polyA-anchored LD primers (Table S1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 3 μg of total RNA was reverse transcribed to cDNA with Oligo(dT)15 primers using PrimeScript™ Reverse Transcriptase (Takara Bio, Shiga, Japan). The resultant cDNA was subjected to real-time PCR by using SYBR® Premix Ex Taq™ II (Takara Bio ...
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bioRxiv - Biochemistry 2019Quote: ... RACE-PCR was conducted with combination of fat body 3’RACE ready cDNA and RACE primers (Table S3) according to the manufacturer’s protocol (Takara Bio, Mountain View, CA, USA). The ORF was obtained by assembling the RACE fragment and the corresponding fragment obtained from the transcriptome ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was used to prepare cDNA using the Ovation RNA-Seq System via single primer isothermal amplification (#7102-A01; Nugen, Redwood CIty, CA, USA) and automated on the Apollo 324 liquid handler (Takara Bio, Kusatsu, Shiga, Japan). cDNA was quantified on the Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplification was performed with primers ANamyD-dGPI-Fw and ANamyD-dGPI-Rv from the resulting pAHT-amyD plasmid with PrimeSTAR Max DNA Polymerase (Takara Bio Inc., Kusatsu, Japan). The amplified fragment was transformed into DH5α competent cells ...
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bioRxiv - Immunology 2020Quote: cDNAs encoding EqPD-1 and EqPD-L1 were amplified by PCR using gene-specific primers with restriction enzyme cleavage sites (Supplementary Table 1) and subcloned into the multicloning site of pEGFP-N2 (Clontech, Palo Alto, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... transcripts of the target genes were amplified with the corresponding primer pairs (S2 Table) and quantified with SuperReal PreMix Plus (SYBR Green) (Takara biomedical technology, Beijing, China), using the 18S rRNA was used as control ...
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bioRxiv - Molecular Biology 2023Quote: ... tabaci adults was used for the synthesis of first strand cDNA utilizing the make your own mate & plate library system with CDS III adaptor primer and smart III oligo (template switching) as per manufacturer’s instructions (Takara Bio, San Jose, CA, USA). The first strand cDNA was used as template for long distance PCR with 5’ and 3’ primers ...
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bioRxiv - Cancer Biology 2023Quote: Coding regions of the heavy-chain variable domains were amplified with two PAGE-purified primers (CALL001: 5’-GTCCTGGCTGCTCTTCTACAAGG-3’ and CALL002: 5’-GGTACGTGCTGTTGAACTGTTCC-3’) using LA Taq polymerase (TAKARA Bio Inc., Shiga, Japan). The amplified fragments were separated on a 1.5% low-melting-temperature agarose gel (Lonza Group AG ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of total RNA was used to synthesize cDNA using Oligo dT primers and Smart MMLV Reverse Transcriptase kit (Takara Bio Inc, Shiga, Japan). The expression of NIa-Pro was verified in all samples using gene specific primers and RT-PCR (Fig S1a ...
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bioRxiv - Molecular Biology 2021Quote: ... was reverse-transcribed and RT-qPCR was carried out on the triplicate samples in an SYBR Green reaction mix (Takara Biotechnology, Shiga, Japan) with an ABI Quant Studio5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2019Quote: First strand cDNA was synthesized from 1 μg total RNA by using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Kusatsu, Japan). qRT-PCR was performed in a 25 μl reaction volume with SYBR Premix Ex Taq II Tli RNaseH Plus (Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... 1.0~5 μg of total RNA was reverse-transcribed in a 20-μl reaction containing 1x RT buffer (Clontech, Mountain View, CA, USA), 0.5 mM dNTPs ...
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bioRxiv - Molecular Biology 2021Quote: ... One µg of total RNAs was subjected to reverse transcription into cDNAs using PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, cat. no. RR047A) according to manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: ... 1.0~5 μg of total RNA was reverse-transcribed in a 20-μl reaction containing 1x RT buffer (Clontech, Mountain View, CA, USA), 0.5 mM dNTPs ...
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bioRxiv - Developmental Biology 2021Quote: ... and the total RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, RR047A, Kusatsu, Shiga, Japan). The diluted cDNA was used as a template for qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized with a PrimeScript™ RT synthesis kit with one-step genomic gDNA eraser kit according to instructions (TaKaRa, Tokyo, Japan). Gene-specific primer sets were designed based on the ORF ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). SYBR Premix Ex Taq II (TaKaRa ...
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bioRxiv - Microbiology 2021Quote: ... The extracellular SARS-CoV-2 RNA in the culture supernatant was analyzed using SARS-CoV-2 direct detection RT-qPCR kit (RC300A; TaKaRa-Bio, Shiga, Japan). The infectivity of SARS-CoV-2 in the culture supernatants was determined at 48 h post-infection ...
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bioRxiv - Genetics 2020Quote: ... RNA was extracted from samples using TRIzol and cDNA synthesized from 1 μg RNA template using PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA BIO INC). The sequences of primers used for amplification are shown in Table S6 ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA (1 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Cat#: RR047A, TaKaRa Bio, Shiga, Japan). qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was reverse transcribed from 500 ng of total RNA with hexamers using a PrimeScript RT reagent kit (Takara Bio, Inc., Kusatsu, Japan). qPCR was then performed in a 25 μl reaction using TB Green Premix Ex Taq II reagent (Tli RNaseH Plus ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The cDNA was synthesized using 1 µg of RNA from each treatment using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa Dalian, China). RT-qPCR was performed on a Bio-Rad CFX96 (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 µg of total RNA was used for reverse transcription using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Beijing, China). After the transcribed cDNAs were 5-fold diluted ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from LNs and used for RT-qPCR using a Prime Script™ II Strand cDNA Synthesis kit (Takara, Dalian, China), in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Equal amounts of total RNA were used to synthesize cDNA with the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara RR047A, China). Quantitative real-time reverse-transcription PCR (qRT–PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal quantities (5 ng) of total RNA from each sample were used for cDNA synthesis using the PrimeScript®RT reagent kit (TaKaRa, Tokyo, Japan). The reverse transcriptions of miRNAs were performed using looped miRNA-specific RT primers for miRNAs ...
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bioRxiv - Bioengineering 2023Quote: Real-time quantitative (RT-qPCR) analysis was performed on the cDNA samples using the Premix Ex TaqTM (Tli RNaseH Plus) (TaKaRa Bio, Dalian, China) and ABI Quantstudio 5 Detection System (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The first-strand cDNAs of mRNA and mature miRNA were synthesized from 500 ng of total RNA using PrimeScript™ RT reagent kit with gDNA Eraser (Cat# RR047A, Takara, Kyoto, Japan) and miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Cat# KR211 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1μg of isolated RNA was taken from each sample was then transcribed to cDNA using PrimeScript ™ RT reagent kit with gDNA Eraser (Takara Bio RR047B). According to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and 12 ng of total RNA was reverse-transcribed to generate cDNA using PrimeScript RT Master Mix (Perfect Real Time; TaKaRa Bio, Shiga, Japan) after cell sorting ...
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bioRxiv - Neuroscience 2023Quote: ... the brain slices were first blocked in 10% goat serum made in 0.1% Triton X-100/1× PBS and then incubated in anti-GFP antibodies (1/1000, RT, overnight, Mouse anti-GFP, 632381, Takara Bio USA, WI) followed by Alexa-488 secondary antibodies (1/200 ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained by reverse-transcribing the total RNA extracted from HCT116 cells using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Dalian, China) was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA from a microgram of RNA was obtained using a PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan) following its instructions ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was removed from 1 μg purified RNA using gDNA Eraser (PrimeScript RT Reagent Kit, Perfect Real Time) following manufacturer’s instructions (Takara Bio Inc., Kusatsu, Japan). Reverse transcription and cDNA was synthesized using PrimeScript Reverse Transcriptase (TaKaRa Bio) ...