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Citations for Takara Bio :
2151 - 2188 of 2188 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... or its derivatives with appropriate antibiotics resistant genes (ampicillin for Escherichia coli and neomycin or puromycin for mammalian cells) and a tag (SNAP-tag or HaloTag) using an In-Fusion HD Cloning Kit (639635, Takara Bio, Shiga, Japan). The E ...
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bioRxiv - Immunology 2022Quote: ... Viruses were packaged in human embryonic kidney (HEK) 293 cells and viral supernatants were processed using Retro-X concentrators (Takara Bio. USA Inc). Naïve CD8 T cells were purified by negative-selection and stimulated in vitro with plate-bound anti- CD3/CD28 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lentiviral supernatants were subsequently collected after transfection of HEK 293T cells with either the pLVX-TRE3G-GOI or the pLVX-Tet3G vector using the X-fect reagent (Clontech; Cat. No. 631317). Supernatants were concentrated by ultracentrifugation ...
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bioRxiv - Microbiology 2020Quote: ... One hundred microliter cell culture supernatant was harvested for viral RNA extraction using the MiniBEST Viral RNA/DNA Extraction Kit (Takara, Cat no. 9766) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was prepared from EV RNA using the Single Cell RNA kit SMART-Seq_V4 Ultra Low Input RNA Kit (634890, Takara, Mountain View, CA, USA) according to manufacturer instructions using 1ng input ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Gastruloids were prepared by aggregating 300 mouse embryonic stem cells in 40 μl droplets of N2B27 (NDiff® 227, Takara Bio Inc. Y40002) per well of U-bottomed 96-well plates (Greiner 650185 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were cultured at a density of 1 × 106 per well in a 6 well dish and the cell number was counted daily under microscopy using trypan blue staining (Takara, Japan. Cat# Y50015). Cells were also seeded at a low density in 96 well plate (5000 cells per well ...
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bioRxiv - Cancer Biology 2021Quote: ... CRISPR/Cas9 plasmid was virally transduced into cells using Lenti-X Packaging Single Shots (VSV-G) using manufacturer’s instructions (Clontech, California, USA, Cat #631275). See Table S3 for list of all gRNAs utilized.
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bioRxiv - Immunology 2021Quote: ... pCSIIhyg-R-GECO1 or pCSIIbsr-GCaMP6s was cotransfected with psPAX2 and pCMV-VSV-G-RSV-Rev into Lenti-X 293T cells (Clontech, Mountain View, CA) with Polyethylenimine “Max” (Mw 40,000 ...
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bioRxiv - Immunology 2021Quote: ... T cell receptor sequencing libraries were prepared with the SMARTer Human TCR α/β Profiling Kit (catalog number 635015, Takara Bio USA, Inc.) according to manufacturer’s instructions with the exception of excluding the third and fourth bead size selection steps listed in Table 3 of the kit manual ...
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bioRxiv - Immunology 2020Quote: In-vivo and in-vitro generated CD11b+ Gr1+ MDSCs were sorted on flow cytometry machine and cells pelleted for RNA isolation using RNAiso plus (Takara bio. Inc., Japan). The extracted RNA was converted to cDNA with Takara kit (Takara bio ...
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bioRxiv - Biophysics 2022Quote: ... were mixed to a final volume of 150 µL and incubated at room temperature for 30 min before adding to the Lenti-X™ 293T Cell Line (Takara, Cat. #: 632180). Media (10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected cells were cultured for over 3 weeks in complete RPMI-1640 medium containing G418 (600 μg/ml; Clontech, Palo Alto, CA, USA) to select resistant clones.
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was produced by transfecting the plasmids in HEK293T cells using Lenti-X™ Packaging Single Shots (Takara Bio, Kusatsu, Shiga, Japan, #631278). The media containing lentivirus were collected from transfected cells and were further concentrated by 50-fold using Lenti-X Concentrator (Takara ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1.25Lμg pVSV-G (second-generation lentivirus packaging system) into human embryonic kidney packaging cells GP2-293 (Clontech, Inc., Mountain View, CA, USA), using CalPhos™ Mammalian Transfection Kit (Clontech ...
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bioRxiv - Microbiology 2023Quote: ... packaging cells were transfected with expression vectors (pMSCVneo-fePit1, pMSCVneo-fePit2, or pMSCVneo empty vector) using the TransIT®-293 reagent (Takara, Kusatsu, Japan). Two days later ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
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bioRxiv - Cancer Biology 2023Quote: ... obtained by reverse-transcribing the total RNA extracted from HCT116 cells using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Dalian, China) was used as the template for amplifying the corresponding regions using the PrimeSTAR Max DNA Polymerase (Takara Bio) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we then added 1 mL of Super Optimal Broth with Catabolite Repression Medium (SOC, provided with the electrocompetent cells (Takara, Japan, product #9027), shaking the cells at 230 RPM at 37°C for 1.5 hours (Infors HT ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were generated in HEK 293T cells by co-transfection of the lentiviral vector pLVX-luc-IRES-puro (Clontech, Mountain View, CA) with the pCMV-dR8.9 and pCMV-VSV-G plasmids (both obtained from Addgene ...
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bioRxiv - Molecular Biology 2019Quote: HEK293 cells were transfected with p122RhoGAP/DLC-1 siRNA (sense, 5′-GAAACGCCUUAAGACACUATT-3′; antisense, 5′-UAGUGUCUUAAGGC GUUUCTT-3′; TaKaRa Biotechnology Co., Ltd., Kyoto, Japan), IQGAP1 siRNA (s16837 ...
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bioRxiv - Immunology 2020Quote: TZM-bl (kindly provided by Dr. Quentin Sattentau, University of Oxford) and Lenti-XTM 293T cells (Takara Bio, Clontech, Saint Germain en Laye, France) were cultured in either complete DMEM or complete DMEM F-12 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: Retroviral supernatant was collected two and three days after transfection of GP2-293T cells and concentrated onto wells of a 6 well plates coated with Retronectin (Takara Bio, 5 ug/ml) by spinning at 2500g for 90 minutes at 32° C ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 x105 cells/cm2 were immediately added to each well with HEK293 maintenance media modified with 10% tetracycline-free FBS (Takara Bio, Cat. No. 631367). After 24 h ...
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bioRxiv - Immunology 2024Quote: ... one containing Mu Mx1 and one containing the Tol2 transposase were transfected into DF1 cells at 50-70% confluency using Xfect transfection reagent (Takara Bio, San Jose, CA) at a 5:1 ratio respectively ...
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bioRxiv - Molecular Biology 2020Quote: A549 cells were obtained from ATCC and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS, Takara Biomedical Technology Co., Ltd., China), 100 U/ml penicillin and 100 U/ml streptomycin (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The Northern dot blots containing cDNAs from primary human tumors (T) with paired adjacent normal tissues (N) and cancer cell lines were obtained from Clontech (Cancer Profiling Array, #7840-1). The blots contained normalized cDNA isolated from tumors and the corresponding adjacent normal tissues from individual cancer patients ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio, Mountain View, United States). MeWo cells (kind gift from Graham Ogg ...
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bioRxiv - Microbiology 2019Quote: ... 1.5 μg of bait plasmids were added into 100 μl of yeast competent cells with 50 μg of denatured Yeastmaker Carrier DNA (Takara Bio USA, Mountain View, CA) and 500 μl PEG/LiAc ...
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bioRxiv - Neuroscience 2022Quote: ... Immunofluorescent staining was performed using primary antibodies (FOS, #2250, Cell Signaling Technology, 1:1000; GFP, GFP1020, Aves Laboratories, 1:1000; dsRed, 632496, Takara, 1:1000), antibodies were reacted with species-specific Alexa Fluor-488 ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T or UFSP2 KO HEK293T cells (16) were transfected with the aforementioned pX330 vectors together with pEGFP-C1 (#6084-1, Clontech Laboratories, Mountain View, CA, USA) and cultured for 2 d ...
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bioRxiv - Neuroscience 2023Quote: ... In-Fusion HD enzyme premix was added to the linearized PCR product and transformed into the Stellar™ Competent Cells (Takara; Bio Inc., Otsu, Japan). Integrity of plasmid sequences was verified through sequencing.
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bioRxiv - Systems Biology 2023Quote: ... was used as the wild-type (WT) strain for genetic manipulations. Chemically competent Escherichia coli (E. coli) DH5α and HST08 (Stellar Competent Cells, TaKaRa Bio Inc., Kusatsu, Shiga, Japan) were used as the host strains for molecular cloning.
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bioRxiv - Cancer Biology 2024Quote: We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC-97L cells using the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... at a molar ratio of 2:1:1 and transfected into the Lenti-X™ 293T packaging cell line (632180, TaKaRa Bio Inc., Kusatsu, Shiga, Japan) using Xfect™ Transfection Reagent (631317 ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were collected at 24 and 48 h after transfection of HEK 293/17 cells and concentrated using a LentiX concentrator (Takara Bio USA, Inc., Mountain View, CA). HEK 293/17 cells or NSC cells were plated in 6 well plates at a density of 105 per well ...
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bioRxiv - Cancer Biology 2023Quote: ... in a molar ratio of 2:1:1 and transfected into the Lenti-X™ 293T packaging cell line (632180, TaKaRa Bio Inc., Kusatsu, Shiga, Japan) using Xfect™ Transfection Reagent (631317 ...
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bioRxiv - Neuroscience 2023Quote: ... We obtained 20 ng/ul RNAs (total 10 ul eluted RNA from one microdissected cell body) that we used for RNAseq (Clontech SMART-Seq Ultra Low Input RNA kit) in Scripps Florida Genomics Core (Currently known as The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology) ...