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Citations for Takara Bio :
2051 - 2100 of 2188 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The retroviral transfer vector was co-transfected with pVSV-G retroviral envelope-expressing plasmid into the GP2-293 cell line (Clontech-TaKaRa) using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... Eed KO and Suz12 KO ES cells were transfected with FLAG-tagged EED or SUZ12 using Xfect™ mESC Transfection Reagent (631320, Clontech) according to manufacturer’s instruction.
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bioRxiv - Cell Biology 2020Quote: ... foetal bovine serum or 10% (v/v) TET System–approved FCS for U–2–OS reporter cell lines (631106, Takara Bio). U–2–OS-pEP15 cells (5 ...
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bioRxiv - Cell Biology 2020Quote: Stable cell lines were made by lentiviral transduction as described above using the Lenti-X Single shot lentivirus packaging system (Takara, #631275) in combination with the lentiviral vector pLVXpuro-proSBP-GFP-COL1A1 (Addgene #110726 ...
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bioRxiv - Biophysics 2020Quote: ... Transformed yeast cells were cultured at 30°C in uracil dropout synthetic defined medium (-Ura dropout supplement was purchased from Takara Bio). To induce expression of genes under the control of the GAL1 promoter ...
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bioRxiv - Microbiology 2021Quote: ... His-Raf1, His-ATG8) and empty plasmids (GST tag, 6×His tag) were transformed into component cells Escherichia coli BL21 (Takara, 9126) or BL21 (DE3 ...
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bioRxiv - Microbiology 2020Quote: ... Single-round replication-incompetent HIV-1 produced from J-Lat-EnTr cells was harvested 48hr post TNF-α treatment and concentrated via Lenti-X-Concentrator (Clontech). J-Lat-EnTr-BFP cells co-cultured with Jurkat-GFP cells produced single-round replication-incompetent HIV-1 produced via TNF-α treatment for 72hr ...
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bioRxiv - Immunology 2021Quote: ... approximately 500 sorted cells were processed directly to generate cDNA using the Clontech SMART-Seq v4 Ultra Low Input Kit (Clontech, Inc.) on three biologically-independent replicates ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 10 ng DNA from the ε2/ε2 and ε3/ε3 cell lines with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Immunology 2021Quote: ... activated CD8+ T cells were transduced with MSCV-ASNS-OE or MSCV-EV viral particles on RetroNectin-coated plates (Takara Bio). 24 hours later ...
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bioRxiv - Immunology 2021Quote: ... The coding sequences of 14-3-3 and βTRCP were amplified by using cDNAs derived from HeLa cell as templates and inserted into vectors above using In-Fusion HD Cloning Kit (Takara Bio). For Myc-IRAK1 expression vector ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: The inducible cell lines expressing WT or mutants of vimentin were established by the Tet-One Inducible Expression System (Clontech, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from the captured cells as per the manufacturer’s protocol using SMART-Seq Ultra Low RNA (Takara Bio; 634833) before being processed for Illumina sequencing via the Nextera XT DNA Library Preparation kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... packaging (pCAG-HIVgp) and envelope (pCMV-VSV-G-RSV-Rev) plasmids were co-transfected into Lenti-X 293T cells (Takara Bio) using Fugene 6 reagent (Promega) ...
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bioRxiv - Immunology 2022Quote: Doxycycline (Dox)-inducible gene-expressing cell lines were generated using Retro-X™ Tet-On 3G Inducible Expression System (Takara Bio). Plat-GP cells were transfected with the pRetroX-Tet3G plasmid together with the packaging plasmid pCMV-VSV-G using Lipofectamine 2000 ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from HT-29 cells using the CellAmp Direct RNA Prep Kit for RT-PCR (3732; TaKaRa, Japan) after the introduction of genomic DNA or Poly (I ...
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bioRxiv - Neuroscience 2022Quote: ... The amplified linear PCR products containing the desired modification were ligated and transformed into Stellar™ Competent Cells (Takara Bio USA). All sequences were confirmed by DNA sequencing (UC Berkeley DNA Sequencing Facility).
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bioRxiv - Cell Biology 2023Quote: ... The media containing lentivirus were collected from transfected cells and were further concentrated by 50-fold using Lenti-X Concentrator (Takara, #631231). RAB12 KO A549 cells were infected with lentivirus expressing WT 3XFLAG-RAB12 or 3XFLAG-RAB12 S106A mutant ...
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bioRxiv - Microbiology 2023Quote: ... a complete cDNA sequence of bovine hnRNPM (NM_001191223) was obtained from genomic DNA of MDBK cells using PrimeScript RT reagent Kit (TaKaRa, Shiga, Japan), followed by a PCR reaction using PrimeSTAR Max DNA Polymerase (TaKaRa ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... cDNA was prepared from 1000 cell equivalent of RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio), and next-generation sequencing libraries were generated using the Nextera XT DNA Library Preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... The single isoform of DCR1 was amplified by RT-PCR from mRNA isolated from S2 cells and reverse transcribed using the Oligo(dT)-primed RNA to cDNA EcoDry premix (Takara Bio). The CDS for AGO1 and GW182 were PCR amplified from pAFW-Ago1 (Addgene #50553 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants from rescue plates were passaged on 15 cm plates of HEK 293T/17 cells (ATCC) transfected with pLV-TTBG and pLV-TTBL (described above) using Xfect transfection reagent (Takara 631318) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The viral particles were purified from transfected cells and titrated using the AAVpro Purification Kit Maxi and AAVpro Titration Kit (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Bait and prey plasmids were transformed into Y187 and Y2H Gold cells according to the manufacturer’s recommendations (Takara Bio USA, www.takarabio.com). Bait and prey combinations were made by mating Y187 bait and Y2H Gold prey strains according to the manufacturer’s recommendations (Takara Bio USA ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were sorted by FACS as described above into 96-well plates containing 0.1 μl of RNase inhibitor (Clontech, Cat# 2313A), 1.9 μl of 0.2% Triton X-100 (Sigma ...
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bioRxiv - Genomics 2023Quote: ... of the resulting ligations as well as the control were transformed into 10 ul of Stellar chemically competent cells (Takara #636763) using manufacturer guidelines ...
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bioRxiv - Developmental Biology 2023Quote: ... The plate containing dried beads bound to single-cell DNA was extracted from the robot and processed following PicoPLEX® (Sopachem/Takara) protocol with half volumes as described in Macaulay et ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T expressing either iRhom2-1-374 or wild-type iRhom2 under TET-inducible cells were generated by lentiviral infection using gene cloned into pLVX-TetOne vector (Clontech, #631849). Methodology is similar to retroviral transduction ...
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bioRxiv - Systems Biology 2023Quote: ... Genomic DNA was extracted from cell pellets using the Machery Nagel NucleoSpin Blood Kit (Midi, Clontech Cat. #740954.20; XL Maxi, Clontech Cat. #740950). The detailed protocol is available at http://www.broadinstitute.org/rnai/public/resources/protocols.
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) in the supernatants from either cell type was quantified using the LDH Cytotoxicity Detection kit (TaKaRa or Roche) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... each egg cell sample was transferred to a lysis buffer supplied in the SMART-Seq HT kit (Takara Bio, Shiga, Japan). The lysates were immediately used for cDNA synthesis or frozen in liquid nitrogen and stored at -80 °C ...
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bioRxiv - Molecular Biology 2023Quote: HeLa (cTT20.1166, gift from Kara L. McKinley and Iain Cheeseman) and Lenti-X HEK293T cells (Takara Bio, used for lentivirus production) were cultured in DMEM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from cells and reverse-transcribed to cDNA using the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa Bio). cDNA was used as a template for real-time qPCR analysis on CFX96 Touch (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transfected with each of the above-mentioned vectors and Lenti-X Packaging Single Shots (VSV-G) (Clontech, 631275) in the presence of reduced-serum Opti-MEM medium (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP 61,62 (400ng) using TransIT-LT1 (Takara, Cat# MIR2306). On day 3 (24 hours post transfection) ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from vehicle and Lantana camara leaf ethanolic extract-treated MDA-MB-231 cells using RNAiso Plus (Takara, Japan) as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR for pluripotency and trilineage spontaneous differentiation was performed according to the instruction manual of the human ES cell Primer Array (Takara Clontech).
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bioRxiv - Cancer Biology 2023Quote: ... and clonally isolated p53 KO cell lines were electroporated with 3 µg p53 firefly luciferase reporter plasmid pp53-TA-Luc (Clontech/Takara) and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were incubated at 3×104 cells/well in 96-well U bottom plates for 24 hours in T cell media containing 50 IU/mL rhIL-2 and varying concentrations of AP20187 (B/B homodimerizer, Takara, 635058). After 24 hours ...
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bioRxiv - Cell Biology 2023Quote: Plasmids NLAD8 HIV-1 AD8 Macrophage-Tropic R5 (NIH AIDS reagents #11346) or HIV Gag-imCherry V3 loop (29) were transfected in HEK 293T cells using CalPhos Mammalian Transfection Kit (Takara Bio) in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... the transfer plasmid (pBABEpuro-based or pLZRS-IRES-ΔNGFR/GFP-based) and pCMV-VSV-G were co-transfected into GP2-293 packaging cells using CalPhos mammalian transfection kit (Takara/Clontech) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... suspensions of the transformants (1.0×106 cells) were spotted onto SD plate media with dropout supplements,-Leu/-Trp or-Ade/-His/-Leu/-Trp (cat #630428) (Takara Bio), and incubated at 30°C for 3 days.
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bioRxiv - Cell Biology 2024Quote: Total RNAs from HeLa cells (about 5 x 107 cells/10 cm dish) transfected with scrambled siRNA (si-Ctrl) or si-CDC5L were extracted using RNAiso Plus (Takara, 9109) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2024Quote: AAV-vector plasmid and AAV-crTSG were used for AAV9 production and purification using Lenti-X 293 T cells (Takara Bio). Each set of Lenti-X 293 T cells (6 150mm-plates ...
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bioRxiv - Cancer Biology 2023Quote: ... In short: 3.5 cm non-coated cell culture dishes (Falcon, cat 351008) were coated using 12 µg Retronectin (Takara, cat T100B). Coated dishes then were pre-incubated with 1 ml viral supernatant for 4-5 hours at 37oC ...
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bioRxiv - Cancer Biology 2024Quote: ... 4T1 cells were transduced with a luciferase cDNA cloned into the pQCXIN retroviral expression vector (Takara Bio USA; San Jose, CA), selected with 0.5 mg/ml G418 ...
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bioRxiv - Developmental Biology 2023Quote: ... Only 0.5 µL of media containing cumulus cells from each mouse was added to 10 µL lysis buffer (Takara Bio Inc.). The oocytes were then treated with 1 mg/mL collagenase to remove the ZP ...
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bioRxiv - Developmental Biology 2024Quote: ... for 20 minutes on ice and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara ...
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bioRxiv - Microbiology 2024Quote: ... reactions were performed to insert these linear fragments into the pMV306 previously digested with EcoRV and transformed into Escherichia coli (E. coli) Stellar TM competent cells (Takara Bio), purified (NucleoSpin Plasmid ...