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Citations for Takara Bio :
1901 - 1950 of 2188 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from cells or tumors using a Mini BEST Universal RNA Extraction Kit (Takara, Shiga, Japan). cDNA was prepared from total RNA by reverse transcription using oligo-dT primers (Takara) ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA encoding Lyn was obtained from RBL-2H3 cells and subcloned into the pTRE2hyg vector with the cDNA encoding FKBP2 and EGFP (derived from pEGFP-N2; Clontech) to produce Lyn-FKBP2-GFP (Lyn-FG) ...
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bioRxiv - Developmental Biology 2021Quote: ... and we immediately used these cells for reverse-transcription and cDNA amplification with a Smart-Seq HT kit (Cat# 634437, Takara). The cDNAs (1 ng each ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from cells or tissues with TRIzol Reagent (Life) and then used for reverse transcription by PrimeScriptTM RT Master Mix (TaKaRa). Synthesized cDNA was used for qPCR by 2× ChamQTM Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Neuroscience 2021Quote: ... Groups of 6-10 cells were dispensed from the micropipette into 1 µl ice-cold 10x reaction buffer (SMART-Seq v4 kit, Takara) and flash-frozen before library preparation ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell lines of each genotype were tested for CARD14 expression in the presence of 500 ng/ml Dox (Clontech), and clones with the highest fold induction were selected for further analyses.
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bioRxiv - Molecular Biology 2021Quote: TET-ON stable HEK293 cells with the Tetracycline-inducible expression constructs were grown in DMEM supplemented with 10% TET-approved FCS (Clontech) and induction of expression of respective gene product was carried out for indicated time durations using 400 ng/ml Doxycycline (SIGMA) ...
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bioRxiv - Genomics 2021Quote: The NucBlue and DAPI co-stained single-nuclei suspension (60□cells/µl) was distributed to eight wells of a 384-well source plate (Takara) and then dispensed into a barcoded SMARTer ICELL8 3’ DE Chip (Takara ...
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bioRxiv - Developmental Biology 2022Quote: ... Three transformation reactions were performed with 2 µl of the reaction mixture in 50 µl Stellar− Competent Cells (636763, Takara) each according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2019Quote: ... Inducible U2OStx cells overexpressing different transgenes were obtained by stable transfection with AhdI-linearized pcDNA4/TO vector using Xfect (Clontech). Resistant clones were selected with 50 µg/ml hygromycin and 100 µg/ml zeocin (Invivogen ...
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bioRxiv - Cancer Biology 2019Quote: ... NEK9 lentiviral particles for each construct were harvested from transfected 293T packaging cells using the Lenti-X(tm) HTX Packaging System (Clontech), transduced into U2OS parental cells and selected in fresh media supplemented with 2 mg/ml of G418 and 1 µg/ml puromycin over several days ...
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bioRxiv - Microbiology 2019Quote: ... The IL-1β and components of RISC mRNA expression levels of the NA cells were quantified with the SYBR Green qPCR kit (Takara) by following the manufacturer’s instruction using gene-specific primers (S2 Table) ...
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bioRxiv - Systems Biology 2020Quote: We extracted RNA from fixed cells after barcode RNA FISH and sorting using the NucleoSpin total RNA FFPE XS kit (Takara). We performed cell lysis and reverse cross-linking at 50°C for 90 minutes and otherwise followed the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... All lentiviral particles other than those used in the RAW cell transductions and Figure 2B (top panel) were further purified and concentrated with the Lenti-X Concentrator reagent (Takara) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... 5′-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Single cells were sorted into 96-well plates containing 4uL lysis buffer containing 4U Recombinant RNase Inhibitor (Takara Bio 2313B), 0.05% Triton X-100 ...
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bioRxiv - Biochemistry 2020Quote: ... U-[15N,12C,2H]-labelled REC3 domain was overexpressed in BL21(DE3) cells containing chaperone plasmid pG-KJE8 (TAKARA, 3340) in M9 medium in 2H2O containing 2 g l−1 2H12C glucose (Sigma #552003 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were seeded at 25-30% confluency onto the prepared coverslips placed in 6-well plates in cell imaging media comprising phenol-red free DMEM (HyClone) supplemented with 10% (v/v) FBS (ClonTech) and 1 mM sodium pyruvate and allowed to settle for at least 4h ...
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bioRxiv - Immunology 2021Quote: ... 5’ s-RACE cDNA was obtained from bulk-sorted B cells of each mouse with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The IgG PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... transfected cells were treated with 200 µg/mL hygromycin B (Nacalai Tesque, Kyoto, Japan) or 1 µg/mL puromycin (Clontech) for the selection of stably transfected cells.
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bioRxiv - Biochemistry 2021Quote: ... pellets were lysed using an SLM Aminco Pressure Cell Press and the clarified lysate was applied onto a HisTALON Superflow column (Clontech). Bound proteins were eluted by applying an increasing imidazole concentration ...
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Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... bacterial cells harvested from fresh culture were embedded in agarose gels and then digested by S1 nuclease (TaKaRa, Dalian, China) at 37 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and VSV-G were co-transfected with each lentiviral vector (pCW-RELA-HA, pCW-C11orf95fus1-HA, or pCW-C11orf95-RELAfus1-HA) into Lenti-X 293T cells (Clontech), seeded the day before in 6-well plates at a concentration of 1.2×106 cells per well ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 7.5 μg of pCMV script-vimentin or empty vector using Xfect™ (Clontech Laboratories, USA), according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2022Quote: Cell death was measured by quantification of the lactate dehydrogenase (LDH) release into the cell supernatant using LDH Cytotoxicity Detection Kit (Takara). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 million cells were electroporated with 5 µg of plasmids encoding tested lncRNAs or with of the pEGFP-N3 reporter vector (Clontech) as previously described 30 ...
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bioRxiv - Developmental Biology 2022Quote: Trophectoderm biopsies containing 5-10 cells from blastocyst-stage embryos (n=24) were processed for RNA-seq using a commercial kit (Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: 1000-3000 EpCam+ Trpm5-GFP+ cells from mice at day 43 post-influenza were sorted directly into lysis buffer from Takara SMART-Seq v4 and RNA / cDNA was amplified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were transduced in 24-well plates precoated with recombinant fibronectin (FN CH-296, Retronectin Takara, Clontech, Mountain View, CA) with retroviral supernatants and expanded in complete medium (45% RPMI-1640 and 45% Click’s medium ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... cells were transduced in 24-well plates precoated with recombinant fibronectin (FN CH-296, Retronectin Takara, Clontech, Mountain View, CA) with retroviral supernatants and expanded in complete medium (45% RPMI-1640 and 45% Click’s medium ...
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bioRxiv - Bioengineering 2022Quote: The cells were confirmed to be mycoplasma-negative every few months using a CycleavePCR Mycoplasma Detection Kit (CY232, Takara Bio).
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bioRxiv - Cell Biology 2022Quote: ... JCaM.1-derived cell lines with tetracycline-inducible gene expression system were maintained in RPMI 1640 supplemented with 10 % tetracycline-negative FBS (Clontech). Cells were routinely tested and found negative for mycoplasma and were not STR profiled.
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bioRxiv - Cancer Biology 2022Quote: ... Induction of DSBs in U2OS-DSB reporter cells line was induced by addition of Shield-1 (1 uM, TAKARA 632189) and 4-OHT (200 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... or kinase-dead RIOK2 (Rev-Tre RIOK2123A,246A) or empty vector (Rev-Tre alone) were created from cells engineered with the Tet3G tetracycline responsive transcriptional inducer (Clontech). Prior to collection for experiments ...
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bioRxiv - Cell Biology 2022Quote: ... VSV-G pseudotyped retroviral particles encoding constructs of choice were prepared using standard protocols using GP2-293 packaging cells (Clontech). Viral supernatants were collected and used to infect target cells by centrifugal inoculation (spinoculation) ...
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bioRxiv - Microbiology 2019Quote: Total RNA of the human multiple myeloma cell line KMM-1 was extracted with RNAiso Plus (Takara Bio, Shiga, Japan). A 24-nt RNA ...
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bioRxiv - Immunology 2019Quote: ... 20 cells were sorted directly in cDNA synthesis buffer from the SMARTer Ultra Low RNA Kit for Illumina sequencing (Clontech) and used directly for cDNA synthesis ...
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bioRxiv - Molecular Biology 2019Quote: Coding sequences of rat Rbpms isoforms were PCR amplified from differentiated PAC1 cell cDNA and cloned into XhoI/EcoRI sites of the pEGFP-C1 vector (Clontech) and into EcoRI/XhoI sites of the pCI-neo-3x-FLAG vector (Rideau et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Both CnAOEC and ISO-HAS-B were cultured in Endothelial Cell Growth Medium 2 Kit (Takara Bio, Inc. Kusatsu, Japan). All cells used were routinely tested for Mycoplasma using PCR and were submitted to ICLAS Monitoring Center (Kawasaki ...
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bioRxiv - Bioengineering 2020Quote: ... Amphotrophic retroviral supernatant was collected 72 hr after transfection and used for the infection of Phoenix-eco cells (ATCC) by using Retronectin (Clontech). Transduced Phoenix-eco cells were sorted using either GFP ...
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bioRxiv - Microbiology 2019Quote: ... 5’-RACE cDNA was obtained from bulk-sorted splenic B cells of each mouse with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... was co-transfected with packaging plasmid (pCAG-HIVgp) and VSV-G/Rev-expressing plasmid (pCMV-VSVG-RSV-Rev) into HEK293T cells (Clontech). Cells expressing Involucrin-mCherry were selected by 2 µg/ml Puromycin treatment.
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bioRxiv - Genomics 2019Quote: ... The transfected cells were cultured for 2 days and lentivirus were harvested and concentrated using the Lenti-X concentrator (Takara) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The retroviral transfer vector was co-transfected with pVSV-G retroviral envelope-expressing plasmid into the GP2-293 cell line (Clontech-TaKaRa) using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Full-length mIDO1 gene was PCR amplified from cDNA of IFN-γ-treated ID8 cell line (26) and inserted into the first cloning site in a retrovirus backbone vector (pQCXIX, Clontech-TaKaRa). A fusion gene of codon-optimized Luciferase (Luc2 ...
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bioRxiv - Cell Biology 2019Quote: ... and lentiviral Tre3G-Cas9 and Tet3G particles were produced in HEK293T cells using the Lenti-X HT packaging system (Clontech). Transduced cells were selected with 10 μg/mL puromycin and 400 μg/mL G418 ...
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bioRxiv - Genomics 2021Quote: ... We packaged the cSNAIL genome with AAV variant PHP.eB (pUCmini-iCAP-PHP.eB) in AAVpro(R) 293T cells (Takara, cat #632273). Viviana Gradinaru provided us with the pUCmini-iCAP-PHP.eB (http://n2t.net/addgene:103005 ...
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bioRxiv - Biochemistry 2021Quote: ... Purification procedure was similar to that for HEK 293 cells except for the usage of cobalt-based affinity resin (Talon, Clontech) to capture His-tagged proteins.
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bioRxiv - Microbiology 2020Quote: ... 5′-RACE cDNA was obtained from bulk-sorted B cells of each animal with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa). The Ig PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Thermo Fisher ...