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Citations for Takara Bio :
1551 - 1600 of 2318 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: RNA was purified from transiently transfected HEK293-FT cells by NucleoSpin RNA (Takara) and the concentrations determined by Nanodrop One (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Lentiviral particles were produced in HEK293T Lenti-X cells (Clontech, Mountain View, CA). Cells (5×106 ...
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bioRxiv - Microbiology 2024Quote: ... the infected RAW 264.7 cells were collected using RNAiso Plus (TaKaRa, Dalian, China) for total RNA extracting and quantitative real-time PCR (qPCR) ...
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bioRxiv - Cell Biology 2024Quote: ... transfected cells were treated with 500 nM of A/C Heterodimerizer (Takara #635057) at the onset of live imagining ...
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bioRxiv - Cell Biology 2020Quote: The commercially available Cellartis Human iPS Cell Line 12 (Takara Bio Inc., Kusatsu, Japan) was used as the human iPS Cell line ...
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bioRxiv - Microbiology 2019Quote: ... cells were transiently transfected with 5 µg of the pTRE3G-Luc control vector (Clontech) using Lipofectamine LTX (see above) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from the transfected cell lines using Trizol reagent (Takara, Japan). One-step PrimeScript miRNA cDNA Synthesis Kit (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH-3T3 cells (gift from Bart Vanhaesebroeck) and Lenti-X 293T (Takara Bio Europe) were cultured in high glucose DMEM (Thermofisher ...
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bioRxiv - Cell Biology 2019Quote: ... SMAD2 #3122 and SMAD3 #9523 antibodies (Cell Signalling Technology) and anti-FLAG antibody (Clontech). Horseradish peroxidase-conjugated secondary antibodies and anti-rabbit and anti-mouse antibodies were acquired from Molecular Probes ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 pmol of siRNA is reverse transfected to 7×104 HeLa-Tetoff cells (Clontech) in a 12 well plate using 1.6 µl RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The resultant plasmid was cloned into Escherichia coli JM109 competent cells (TAKARA, Shiga, Japan) and transformed into S ...
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bioRxiv - Microbiology 2019Quote: ... The constructed plasmid was cloned into Escherichia coli JM109 competent cells (TAKARA, Shiga, Japan) and pre-introduced into restriction-deficient S ...
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bioRxiv - Genetics 2019Quote: ... xanthus cells was extracted using RNAiso Plus reagent following the manufacturer’s protocol (Takara, Beijing). The cDNA synthesis used the PrimeScript RT Reagent Kit with random primers ...
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bioRxiv - Immunology 2019Quote: ... Total RNA was isolated from the cells using an RNA Extraction Kit (Takara, Japan) and then reverse-transcribed to cDNA using the PrimeScript RT Master Mix Kit (Takara ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was purified from Ax2 cells with the NucleoSpin Tissue kit (Takara Bio). The following plasmids were gifted by Dr ...
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bioRxiv - Genomics 2019Quote: ... cells were dispensed into a SMARTer ICELL8 350v chip (Takara Bio USA, Cat. # 640019) at 35 nanoliter per well ...
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bioRxiv - Genomics 2021Quote: ... Cells were infected to stably express LY6S-pQCXIP retroviral vector (Clontech Laboratories, Inc., USA) as described previously (45).
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bioRxiv - Synthetic Biology 2021Quote: ... Dynabeads were removed and stimulated T cells were seeded on Retronectin (Takara Bio, Japan) coated nontissue culture treated plate with virus ...
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bioRxiv - Biochemistry 2020Quote: ... the cells were harvested for analysis using Guide-it™ sgRNA Screening Kit (TaKaRa). Stable I2PP2A knockdown CRISPR I2PP2A HEK293 cells were also maintained in MEM with 10% FBS supplemented with Gentamycin (50 mg/ml).
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bioRxiv - Cancer Biology 2021Quote: ... NSCLC cell lines were transfected by the mitochondrion-targeted DsRed (mtDsRed, Clontech, California, USA) plasmid or the enhanced green fluorescent protein (EGFP ...
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bioRxiv - Cancer Biology 2020Quote: ... the lentiviral pCSIIneo or pCSIIbsr vectors were transfected into Lenti-X 293T cells (Clontech) together with the packaging plasmid psPAX2 (a gift from Dr ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from Vero cells using the NucleoSpin RNA kit (Takara Bio Inc.), and cDNA was synthesized with the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio Inc.) ...
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bioRxiv - Immunology 2021Quote: ... Target cell death was quantified with an LDH (lactate dehydrogenase) cytotoxicity assay kit (Clontech) using the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... To encapsulate the construct for transduction we co-transfected Lenti-X 293T cells (ClonTech) with 10µg CENPA-FLAG-HA pLVX-TetOne and packaging vectors 7.5µg psPAX2 and 2.5µg pMD2.G using PolyPlus JetPrime transfection reagent according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 μl premix WST-1 cell proliferation reagent (Takara Bio Inc, Clontech Laboratories, Inc.) was added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... 293FT cells were transfected with the pLV constructs along with packaging plasmids pVSVG (Clontech) and pLV-CMV-delta 8.2 (Massey ...
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bioRxiv - Immunology 2021Quote: ... Retroviral transduction of primary T cells was performed with retronectin-coated plates (Takara Bioscience). Naïve T cells had been activated as described in ‘Cell Cultures’ methods for two days before transduction ...
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bioRxiv - Cancer Biology 2022Quote: ... from cells and reversed transcribed into cDNA using PrimeScript RT Master Mix (TaKaRa, Japan). qPCR was performed to detect mRNA expression of genes by TB Green PCR Kit (TaKaRa ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was isolated from cells using Trizol RNA isolation kit (Takara, Shiga, Japan) and MDR1 and GAPDH transcripts were detected using a TransScript Green One-Step qRT-PCR SuperMix (TransGen Biotech ...
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bioRxiv - Microbiology 2022Quote: Doxycycline-inducible stable cells overexpressing Trim69 were generated using the pRetroX-Tight system (Clontech), a murine leukemia virus (MLV ...
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bioRxiv - Cell Biology 2022Quote: ... coli DH5⊡ competent cells and isolated using a midi-prep kit (Takara Bio, 740420.10), and validated by gel electrophoresis.
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bioRxiv - Plant Biology 2021Quote: ... and cells with both constructs were selected on SD/-Leu/-Trp medium (Clontech, 630417). Ten colonies picked up with a tooth pick were diluted in 1ml 0.9%NaCl and spotted onto SD/-Ade/-His/-Leu/-Trp medium (Clontech ...
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bioRxiv - Neuroscience 2021Quote: ... cells were spinoculated with viral supernatant at 2000xg for 1 hour on retronectin (Takara) coated plates ...
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bioRxiv - Neuroscience 2020Quote: iPSCs cell culture was performed using the Cellartis DEF-CS 500 culture system (Takara) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... the cell supernatant was collected and lysed in lysis buffer (Takara, Cat no. 9766) for further quantification analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T transfected cells conditioned media were condensed with the Lenti-X Concentrator (Clontech: 631231) at 4°C for 24 hours.
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bioRxiv - Immunology 2022Quote: ... GP2-293 retroviral packaging cells and the p10A1 envelope vector were purchased from Clontech. Lipofectamine 3000 was purchased from ThermoFisher and Polybrene was purchased from Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
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bioRxiv - Pathology 2020Quote: Lentiviruses were packaged in Lenti-X 293T cells (cat#632180, Clontech, Mountain View, CA) using a three-plasmid expression system (pLKO.1-shRNAs-puro ...
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bioRxiv - Genetics 2019Quote: Viral RNA was extracted from cell suspensions using a Viral RNA Extraction Kit (TaKaRa) and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: HeLa cells at 40% confluence on coverslips were transfected with pEGFP-N2 (Clontech, USA) constructs encoding variants of BteA-GFP fusion protein (S3 Table ...
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bioRxiv - Molecular Biology 2020Quote: ... These cell lines were tested negative for mycoplasma (TaKaRa PCR Mycoplasma Detection Set; 6601), maintained in RPMI1640 (Nacalai Tesque ...
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bioRxiv - Cell Biology 2019Quote: Total RNA of the cultured cells were harvested with RNAiso Plus reagent (TaKaRa, Japan) following the manufacture’s instruction ...
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bioRxiv - Immunology 2020Quote: ... 106 cells per well were transferred into RetroNectin-coated 24well plates (Takara Bio, USA). Virus supernatant was centrifuged (300xg ...
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bioRxiv - Genomics 2019Quote: ... transduced with concentrated shRNA-expressing lentiviruses harvested from LentiX-293T cells (Takara Bio; 632180), and cultured in BD Matrigel Matrix High Concentration (BD Biosciences ...
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bioRxiv - Genetics 2021Quote: ... HT-29 cells were transduced with the pLVX-Tet3G regulator vector (Takara Bio USA) and a stable Tet-On 3G transactivator-expressing clonal HT-29 cell line (HT-29-pLVX-Tet3G ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated from K562 and GM12878 cells using RNAiso Plus (Takara Bio) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 μl premix WST-1 cell proliferation reagent (Takara Bio Inc, Clontech Laboratories, Inc.) was added to each well ...
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bioRxiv - Cell Biology 2021Quote: The single-cell sequencing library was generated using the ICELL8 cx platform (Takara Bio) and sequencing was performed using the HiSeq 3000 sequencer (Illumina ...
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bioRxiv - Microbiology 2020Quote: Lenti-X 293T™ cells (H. sapiens; sex: female) obtained from Takara (cat. #632180) and Huh-7.5 cells (H ...