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Citations for Takara Bio :
1551 - 1600 of 2188 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were generated by seeding Lenti-X 293T cells (Takara Bio, Cat. No. 632180) in 6-well plates ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was isolated from HeLa cells or flies using RNAiso Plus (Takara, 9109) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: The human embryonic kidney (HEK) 293T cell line was purchased from Takara (Cat# 632180) and cultured in a humidified 95% air / 5% CO2 incubator at 37°C in a standard Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... as well as from various feline cell lines using an RNAiso Plus kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: High-quality RNA was isolated from cells or tissues using Trizol reagent (AJF1807A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... which ultimately was produced with Takara’s Lenti-X™ 293T cells (Clontech, cat. no. 632180) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell homogenates were then subjected to total RNA extraction with Nucleospin RNA plus (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Both soluble and insoluble (cell pellet) fractions were purified via His-IDA nickel column (Clontech Laboratories ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from skin or cell lysates using TRIzol Reagent (RNAiso Plus, TaKaRa). From isolated RNA ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were extracted from yeast cells by Easy Yeast Plasmid Isolation Kit (#630467, Takara Bio) and transformed into E ...
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bioRxiv - Genomics 2020Quote: ... 5 × 104 cells were stored at −80 °C in STEM CELLBANKER® (Takara Bio Inc.) until use ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated from stably transduced cell lines using RNAiso Plus (TaKaRa, Dalian, China) and reverse-transcribed with a PrimeScript™ cDNA Synthesis Kit (TaKaRa ...
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bioRxiv - Developmental Biology 2019Quote: ... was transfected into E14 Bra-GFP cells using Xfect™ transfection reagent (Cat. # 631318, Takara). After selection ...
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bioRxiv - Developmental Biology 2021Quote: ... Seventy percent confluent Lenti-X™ 293T cells (Takara Bio USA, Inc., Mountain View, CA) were transfected with Hdac3 lentiviral plasmids (Hdac3 KO or empty vector control [EV]) ...
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bioRxiv - Cell Biology 2021Quote: ... HA-APOBEC3G was cloned using cDNA from TZMbl cells in pCMV-HA vector from Clontech, USA ...
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bioRxiv - Genomics 2021Quote: ... U2OS cells were grown in DMEM supplemented with 10% tetracycline-free foetal bovine serum (Clontech), 100U/ml penicillin and 100µg/ml streptomycin ...
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bioRxiv - Genomics 2021Quote: ... MYC expression was induced in U2OS cells by the addition of 1µg/ml doxycycline (Clontech) for 30h.
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bioRxiv - Neuroscience 2020Quote: ... Cells were resuspended in 500uL FACS buffer with RNase inhibitor (Takara Bio 2313B, 1:500) and 0.5ul Propidium Iodide (ThermoFisher Scientific P3566 ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was quantified from cell supernatant as a confirmation of the infection by Takara PrimeDirect probe ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Enzyme was then expressed in BL21-DE3 cells with pG-KJE8 chaperone plasmid (Takara Bio) in media supplemented with 0.5 mg/mL arabinose and 5 ng/mL doxycycline to induce chaperone expression ...
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bioRxiv - Bioengineering 2021Quote: ... VSV-G-pseudotyped lentivirus was obtained by co-transfection of Lenti-X 293T cells (Takara) with pRRL-based transfer plasmids as well as pMD2.G (Adgene # 12259 ...
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bioRxiv - Molecular Biology 2020Quote: ... and SD-BRCA1 were harvested and lysed with the xTractor bacterial cell lysis buffer (Clontech) and sonication ...
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bioRxiv - Cancer Biology 2020Quote: ... LSK cells were transduced with a Lhx2 pMSCV-puromycin (Clontech/Takara, Mountain View, CA, USA) vector47 in 1% pegGOLD Universal Agarose (Peqlab/VWR Darmstadt ...
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bioRxiv - Biophysics 2020Quote: ... 50 µl of cell lysate was added to equal volume of LDH substrate (Takara, France) in a 96-well microplate and incubated at room temperature for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... CHO-K1 cells were transfected with ACP-human IR plasmid using Xfect transfection reagent (Takara). 1 day after transfection ...
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: SV40-MES-13 cells were lysed with xTractor Buffer Kit (CLONTECH Laboratories, Inc., CA, USA). Protein specimens were quantified through BCA method and electrophoresed through 10% SDS-PAGE gels ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviruses were generated in HEK293FT cells and concentrated using the Lenti-X-concentrator (Takara Bio).
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bioRxiv - Neuroscience 2022Quote: ... and VSV-G (viral envelope) (Stewart et al., 2003) into Lenti-X HEK293T cells (Clontech) at a mass ratio of 5:4:3 using polyethyleneimine (PEI) ...
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bioRxiv - Microbiology 2022Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, Cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... CHO-K1 cells were co-treated using 1μg/mL doxycycline (Takara Bio Europe, Clontech Inc.) along with the small molecules for experiments as described in main text.
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bioRxiv - Microbiology 2021Quote: ... the CPER products were transfected into HEK293-C34 cells using TransIT-LT1 (Takara, cat# MIR2300) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... and the DNA isolated from the cell lysates using viral DNA extraction kit (TaKaRa, Japan) was used to determine the cleavage activity of HSV-1 genomes by Sanger sequencing and TIDE analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... cell cycle indicator (FUCCI) was cloned from the pRetroX-SG2M-Red Vector (Takara Bio, 631465) and added to the pB-IUE cocktail ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells using the TRIzol RNA isolation reagents (Takara bio, Japan). The first strand cDNA was reverse transcribed from the RNA templates using the GoScript Reverse Transcription system kit (Promega ...
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bioRxiv - Physiology 2019Quote: Cells were plated onto poly-L-lysine-coated coverslips and transfected using the Xfect (Clontech) transfection reagent ...
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bioRxiv - Cell Biology 2020Quote: ... 293FT packaging cells were transfected with lentiviral cDNA using Lenti-X packaging system (631276; Takara). Cultured media containing lentiviral particles was harvested after 72h and used to infect 3T3-L1 preadipocytes ...
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bioRxiv - Genomics 2020Quote: ... half of the cells were treated with 100ng/ml Doxycycline and 1μM Shield1 (Clontech Takara) for 15h ...
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bioRxiv - Genomics 2020Quote: ... half of the cells were treated with 100ng/ml Doxycycline and 1μM Shield1 (Clontech Takara) for 15h ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from A549 cells using a CellAmp Direct RNA Prep Kit (TaKaRa), as well as murine pharyngeal and lung tissues using an RNeasy Fibrous Tissue Mini Kit (QIAGEN) ...
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bioRxiv - Biochemistry 2021Quote: ... Permeabilized cells were then mock treated or treated with 1 mg/ml RNase A (Takara) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... in the T/C-28a2 chondrocyte cell after transfection with a pIRESpuro2 plasmid construct (Clontech) containing the human Cx43 sequence ...
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bioRxiv - Cell Biology 2019Quote: PtK2 GFP-α-tubulin cells (stable line expressing human α-tubulin in pEGFP-C1; Takara Bio Inc. ...
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bioRxiv - Genomics 2021Quote: ... These cells were cultured using Cellartis DEF-CS 100 Culture System (DEF-CS medium; Takara) under feeder-free condition ...
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bioRxiv - Physiology 2021Quote: ... plasmids were transfected into C2C12 cells using Xfect reagent (Cat. No. 631324; Takara Bio, USA) according to manufacturer instructions.
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bioRxiv - Cell Biology 2021Quote: ... CHO-K1 cells were co-treated using 1μg/mL doxycycline (Takara Bio Europe, Clontech Inc.) along with the small molecules for experiments as described in main text.
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bioRxiv - Cancer Biology 2020Quote: ... LSK cells were transduced with a Lhx2 pMSCV-puromycin (Clontech/Takara, Mountain View, CA, USA) vector47 in 1% pegGOLD Universal Agarose (Peqlab/VWR Darmstadt ...
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bioRxiv - Genomics 2021Quote: Hap1 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FCS (Clontech), 1% Penicillin/Streptomycin (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... the lentivirus collected from the cell culture media was concentrated using Retro-X concentrator (Takara). The concentrated lentivirus was used immediately to transduce human NSCLC cell lines ...
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bioRxiv - Genomics 2020Quote: ... in a 1:8 vector:insert optimized ratio and transformed into Stellar competent cells (636766, Clontech) to maximise the number of individual transformants (800,000 individual clones) ...