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Citations for Takara Bio :
1451 - 1500 of 2188 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from tissues and cells was extracted using TRIzol reagent (Takara, Japan) and were reversed transcribed into first-strand cDNA using the PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Genomics 2023Quote: ... were described previously.136 Lenti-X 293T cells were purchased from Takara (#632180). K562 CRISPRi and RPE1 CRISPRi cell lines were authenticated by analysis of short tandem repeats as exact matches to the corresponding line ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from cell pellets using Nucleospin RNA Plus XS kit (Takara), and first strand cDNA synthesis was performed using Superscript IV VILO Master Mix with ezDNAse (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... the mRNAs in these cells were extracted using RNAiso Plus (Takara, Cat:9109) and then sequenced by Biomarker Techonologies using Illumina sequencing technology ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from Mm cells using the TRIzol Reagent (Takara, Japan). cDNA was synthesized using a PrimeScript reverse transcriptase reagent kit (Takara ...
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bioRxiv - Physiology 2023Quote: ... Titering was performed on Ad293 cells using Adeno-X Rapid Titer kit (Clontech) according to the instructions of the manufacturer 28.
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bioRxiv - Neuroscience 2023Quote: ... Cell debris was filtered from the collected medium and Lenti-X Concentrator (Clontech) was then used to precipitate the lentivirus in the medium ...
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bioRxiv - Neuroscience 2023Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), the trans-plasmid providing viral capsid PHP.eB (a generous gift from Viviana Gradinaru ...
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bioRxiv - Genomics 2023Quote: ... cells were switched to media made with Tet System Approved FBS (Takara 631367) to reduce background induction ...
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bioRxiv - Microbiology 2023Quote: ... and MS4 (feline B-cell lymphoma) (73) using an RNAiso Plus kit (Takara), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with the Rapalog A/C hetero-dimerizer rapalog (635057, Takara) for 24 h ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: Using the Lenti-X 293 T cell line (Takara Bio Inc., Otsu, Japan) and the pLVSIN-EF1α-AcGFP-C1 vector ...
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bioRxiv - Neuroscience 2023Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), the trans-plasmid providing viral capsid PHP.eB (a generous gift from Viviana Gradinaru ...
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bioRxiv - Systems Biology 2024Quote: ... We used 600 µl of cell lysate for total RNA extraction (Clontech 740955), followed by processing for microarray (for interactome assembly ...
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bioRxiv - Genomics 2023Quote: ... For lentiviral transduction the cells were plated on retronectin coated plates (Takara Bio) and transduced in the presence of 8μg/ml protamine sulfate (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Lenti-X™ 293T cell line (human, female origin) were purchased from TaKaRa and used without further authentication ...
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bioRxiv - Neuroscience 2024Quote: The total RNA of cells was isolated with RNAiso plus (Cat# 9108, Takara). The concentration and purity of RNA samples were measured using a Nanodrop ND-2000 (Thermo Science ...
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bioRxiv - Bioengineering 2024Quote: ... lentivirus targeting mCherry to the cell membrane (Takara, 0026VCT, rLV.EF1.mCherry-Mem-9) was used according to manufactures recommendation ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified cell lysate was incubated with pre-equilibrated Ni- NTA resin (Takara) for 2 h at 4°C in slow shaking conditions ...
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bioRxiv - Cell Biology 2020Quote: The commercially available Cellartis Human iPS Cell Line 12 (Takara Bio Inc., Kusatsu, Japan) was used as the human iPS Cell line ...
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bioRxiv - Microbiology 2019Quote: ... cells were transiently transfected with 5 µg of the pTRE3G-Luc control vector (Clontech) using Lipofectamine LTX (see above) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from the transfected cell lines using Trizol reagent (Takara, Japan). One-step PrimeScript miRNA cDNA Synthesis Kit (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH-3T3 cells (gift from Bart Vanhaesebroeck) and Lenti-X 293T (Takara Bio Europe) were cultured in high glucose DMEM (Thermofisher ...
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bioRxiv - Cell Biology 2019Quote: ... SMAD2 #3122 and SMAD3 #9523 antibodies (Cell Signalling Technology) and anti-FLAG antibody (Clontech). Horseradish peroxidase-conjugated secondary antibodies and anti-rabbit and anti-mouse antibodies were acquired from Molecular Probes ...
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bioRxiv - Molecular Biology 2019Quote: ... 15 pmol of siRNA is reverse transfected to 7×104 HeLa-Tetoff cells (Clontech) in a 12 well plate using 1.6 µl RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The resultant plasmid was cloned into Escherichia coli JM109 competent cells (TAKARA, Shiga, Japan) and transformed into S ...
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bioRxiv - Microbiology 2019Quote: ... The constructed plasmid was cloned into Escherichia coli JM109 competent cells (TAKARA, Shiga, Japan) and pre-introduced into restriction-deficient S ...
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bioRxiv - Genetics 2019Quote: ... xanthus cells was extracted using RNAiso Plus reagent following the manufacturer’s protocol (Takara, Beijing). The cDNA synthesis used the PrimeScript RT Reagent Kit with random primers ...
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bioRxiv - Immunology 2019Quote: ... Total RNA was isolated from the cells using an RNA Extraction Kit (Takara, Japan) and then reverse-transcribed to cDNA using the PrimeScript RT Master Mix Kit (Takara ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was purified from Ax2 cells with the NucleoSpin Tissue kit (Takara Bio). The following plasmids were gifted by Dr ...
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bioRxiv - Genomics 2019Quote: ... cells were dispensed into a SMARTer ICELL8 350v chip (Takara Bio USA, Cat. # 640019) at 35 nanoliter per well ...
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bioRxiv - Genomics 2021Quote: ... Cells were infected to stably express LY6S-pQCXIP retroviral vector (Clontech Laboratories, Inc., USA) as described previously (45).
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bioRxiv - Synthetic Biology 2021Quote: ... Dynabeads were removed and stimulated T cells were seeded on Retronectin (Takara Bio, Japan) coated nontissue culture treated plate with virus ...
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bioRxiv - Biochemistry 2020Quote: ... the cells were harvested for analysis using Guide-it™ sgRNA Screening Kit (TaKaRa). Stable I2PP2A knockdown CRISPR I2PP2A HEK293 cells were also maintained in MEM with 10% FBS supplemented with Gentamycin (50 mg/ml).
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bioRxiv - Cancer Biology 2021Quote: ... NSCLC cell lines were transfected by the mitochondrion-targeted DsRed (mtDsRed, Clontech, California, USA) plasmid or the enhanced green fluorescent protein (EGFP ...
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bioRxiv - Cancer Biology 2020Quote: ... the lentiviral pCSIIneo or pCSIIbsr vectors were transfected into Lenti-X 293T cells (Clontech) together with the packaging plasmid psPAX2 (a gift from Dr ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from Vero cells using the NucleoSpin RNA kit (Takara Bio Inc.), and cDNA was synthesized with the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio Inc.) ...
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bioRxiv - Immunology 2021Quote: ... Target cell death was quantified with an LDH (lactate dehydrogenase) cytotoxicity assay kit (Clontech) using the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... To encapsulate the construct for transduction we co-transfected Lenti-X 293T cells (ClonTech) with 10µg CENPA-FLAG-HA pLVX-TetOne and packaging vectors 7.5µg psPAX2 and 2.5µg pMD2.G using PolyPlus JetPrime transfection reagent according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 μl premix WST-1 cell proliferation reagent (Takara Bio Inc, Clontech Laboratories, Inc.) was added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... 293FT cells were transfected with the pLV constructs along with packaging plasmids pVSVG (Clontech) and pLV-CMV-delta 8.2 (Massey ...
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bioRxiv - Immunology 2021Quote: ... Retroviral transduction of primary T cells was performed with retronectin-coated plates (Takara Bioscience). Naïve T cells had been activated as described in ‘Cell Cultures’ methods for two days before transduction ...
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bioRxiv - Cancer Biology 2022Quote: ... from cells and reversed transcribed into cDNA using PrimeScript RT Master Mix (TaKaRa, Japan). qPCR was performed to detect mRNA expression of genes by TB Green PCR Kit (TaKaRa ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was isolated from cells using Trizol RNA isolation kit (Takara, Shiga, Japan) and MDR1 and GAPDH transcripts were detected using a TransScript Green One-Step qRT-PCR SuperMix (TransGen Biotech ...
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bioRxiv - Microbiology 2022Quote: Doxycycline-inducible stable cells overexpressing Trim69 were generated using the pRetroX-Tight system (Clontech), a murine leukemia virus (MLV ...
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bioRxiv - Cell Biology 2022Quote: ... coli DH5⊡ competent cells and isolated using a midi-prep kit (Takara Bio, 740420.10), and validated by gel electrophoresis.
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bioRxiv - Plant Biology 2021Quote: ... and cells with both constructs were selected on SD/-Leu/-Trp medium (Clontech, 630417). Ten colonies picked up with a tooth pick were diluted in 1ml 0.9%NaCl and spotted onto SD/-Ade/-His/-Leu/-Trp medium (Clontech ...
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bioRxiv - Neuroscience 2021Quote: ... cells were spinoculated with viral supernatant at 2000xg for 1 hour on retronectin (Takara) coated plates ...