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Citations for Takara Bio :
1401 - 1450 of 2318 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Retroviral supernatants were produced using the GP2-293 packaging cell line (Clontech), in which pRetroX-TetOne puro empty vector or pRetroX-TetOne puro-ALKBH5 were each cotransfected with the envelope vector VSV-G using Xfect transfection reagent (Clontech) ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated from cells using the RNA extraction reagent (Takara). Reverse transcription was performed using PrimeScriptTM RT reagent kit (Takara) ...
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bioRxiv - Biophysics 2022Quote: ... E-cadherin (24E10-Cell Signaling Technology; DECMA1-Sigma Aldrich, ECCD2, Takara Bio) (1:100)and paxillin (Abcam ...
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bioRxiv - Neuroscience 2020Quote: Adeno-associated viruses were made in AAV-Pro HEK293T cells (Takara Bio) by co-transfecting pAAV-GFP or pAAV-GFP-Zdhhc14sh#1 with pAAV2 (pACG2)-RC triple mutant (Y444 ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was generated by transiently transfecting Lenti-X 293T cells (Takara Bio) with lentiviral packaging mix (Cellecta) ...
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bioRxiv - Microbiology 2021Quote: ... and was electro-transformed into E.coli HST08 Premium Electro-Cells (Takara, 9028) according to the manufacturer’s protocol using a Gene Pulser Xcell (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... Genetic manipulations were carried out in Escherichia coli StellarTM competent cells (Clontech). Primers and plasmids used in this study are listed in Table S2 and S3 ...
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bioRxiv - Neuroscience 2021Quote: ... The lentiviruses were packaged in Lenti-X 293T cell line (Takara Bio) with Lenti-X packaging single shots (Takara Bio) ...
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bioRxiv - Molecular Biology 2020Quote: ... Transformations have been performed using Stellar(tm) Competent Cells (Takara, Cat#636763).
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bioRxiv - Immunology 2020Quote: ... The probe-containing vector was then transformed into Stellar Competent Cells (Takara) via manufacturer protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The Gp2-293 packaging cell line was purchased from Clontech (Cat. 631458). Cells were cultured in DMEM (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Cell extract is filtered and incubated with washed TALON beads (Takara 635501) at 4C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Neuroscience 2019Quote: ... The cloned plasmids were transformed into Stellar competent cells (Takara Bio, Japan), and 200 clones per rabies virus sample were isolated and purified for sequencing ...
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bioRxiv - Immunology 2020Quote: Total RNA was obtained from cells with the use of RNAiso (TaKaRa). RT was performed with 1 μg of total RNA and ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo) ...
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bioRxiv - Neuroscience 2019Quote: ... and the PC12 Tet-Off cell line (RRID:CVCL_V361) was obtained from Clontech Laboratories (Mountain View ...
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bioRxiv - Synthetic Biology 2020Quote: ... Lentiviral particles were produced in Gesicle Producer 293T cells (TAKARA ref 632617) transiently transfected by pGY577 (40% of total DNA) ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were then transfected using the Xfect™ Transfection protocol (Takara). The transfected cells were incubated for 48 hours ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was extracted from the cells using Trizol (Takara, Shiga, Japan). Reverse transcription and qPCR were performed in accordance with the manufacturer’s instructions (Vazyme Biotech Co. ...
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bioRxiv - Genomics 2021Quote: AAV was produced in AAVpro(R) 293T cells (Takara, Kyoto, Japan; #632273) by co-transfection of the genome pAAV ...
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bioRxiv - Genetics 2020Quote: ... Mutant cells were detected by competition-based PCR using ExTaq (Takara Bio), which consists of a PCR procedure using three primers ...
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bioRxiv - Immunology 2021Quote: ... Lentivirus was produced using a HEK293T Lenti-X cell line (Takara Bio). HoxB8-conditional progenitors were transduced with lentivirus in the presence of 5 µg/mL polybrene (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... and subsequent colony sequencing of transformed Stellar™ Competent Cells (Takara, 636766). gRNA sequence ...
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bioRxiv - Genetics 2021Quote: ... cDNA synthesis was done by SMART-Seq Single Cell kit (Takara Bio) and the library was prepared with Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293T cells were transfected using the CalPhos mammalian transfection kit (TaKaRa Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Lentivirus for each construct was produced in HEK293T cells (Takara Bio, 632180) using psPAX2 and VSV-G plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T (ATCC CRL-3216) and GP2-293 cells (Takara Bio, Cat. 631458) were maintained in DMEM high glucose supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... The sorted cells were returned to DMEM with TET-free FBS (Takara). MDA-MB-231-mARGAna was sorted twice ...
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bioRxiv - Molecular Biology 2022Quote: ... coli JM109 cell pellets by using NucleoBond Xtra Midi (TaKaRa Bio Inc.) and protocol for low copy plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then treated with 2 μg/mL puromycin (Clontech; 631306) under selection for at least 1 week ...
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bioRxiv - Molecular Biology 2022Quote: ... The assembled plasmids were amplified by transformation of Stellar Competent Cells (Takara) and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2023Quote: ... and pCMV-Rev into 293T cells using TranIT-293 (TaKaRa, Shiga, Japan) or the ViraPower Lentiviral Expression System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted from cells using the TRIzol reagent (Takara, Shiga, Japan) and reverse transcribed using the PrimeScript™ RT reagent Kit (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... the cells were treated with or without 1 μM rapalog (Takara Bio) for one hour prior to fixation ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated with indicated concentrations of B/B homodimerizer (Takara Bio) for 15 h ...
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bioRxiv - Immunology 2024Quote: LDH in cell supernatants was measured using the LDH Cytotoxicity Kit (TaKaRa) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Human naïve cells were cultured in PXGL medium30: N2B27 medium (Takara, Y40002), supplemented with 1uM PD0325901 (Cambridge Bioscience ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... lentiviruses were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... Lentiviral particles were generated by transfection of Lenti-X 293T cells (Takara) with 15 ug LeGO expression vector ...
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bioRxiv - Microbiology 2024Quote: Plasmids were introduced into HEK293T cells using the TransIT®-293 (Takara) reagent in six-well plates ...
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bioRxiv - Neuroscience 2024Quote: ... The In-Fusion reaction mixture were transformed into Stellar Competent Cells (Clontech) using the heat shock Method.
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bioRxiv - Molecular Biology 2024Quote: ... These constructs were transfected into the 293 Ecopack packaging cell line (Clontech) using Lipofection 2000 transfection reagent (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... then cells were selected with 2 μg/ml puromycin (631306; Takara Bio) or 500 μg/ml geneticin (10131027 ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were co-transfected either with pEGFP-N1 (Clontech, 6085-1) or pCAG-DsRed-T1 (gift from Prof ...
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bioRxiv - Microbiology 2024Quote: ... total DNA from fractionated cells was isolated with NucleoSpin Tissue XS (TAKARA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were heat-shock transformed into stellar competent cells (Takara Bio, Kusatsu). SOC outgrowth was performed for 1 hour at 37°C ...
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bioRxiv - Genetics 2024Quote: Lentivirus was produced in the Lenti-X 23T cell line (Takara Bio). Transfection was carried out with psPAX2 (Addgene #12260) ...
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bioRxiv - Cancer Biology 2024Quote: ... H1299 cells were transfected with ten µg of pEGFP-N1 vector (Clontech), containing a C-terminus EGFP-tagged sequence of the full-length M237I p53 protein (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... The tetrazolium salts from Premix WST-1 Cell Proliferation Assay System (TAKARA) are cleaved by mitochondrial dehydrogenase in viable cells to produce formazan dye ...