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Citations for Takara Bio :
1501 - 1550 of 2188 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: iPSCs cell culture was performed using the Cellartis DEF-CS 500 culture system (Takara) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... the cell supernatant was collected and lysed in lysis buffer (Takara, Cat no. 9766) for further quantification analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T transfected cells conditioned media were condensed with the Lenti-X Concentrator (Clontech: 631231) at 4°C for 24 hours.
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bioRxiv - Immunology 2022Quote: ... GP2-293 retroviral packaging cells and the p10A1 envelope vector were purchased from Clontech. Lipofectamine 3000 was purchased from ThermoFisher and Polybrene was purchased from Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
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bioRxiv - Pathology 2020Quote: Lentiviruses were packaged in Lenti-X 293T cells (cat#632180, Clontech, Mountain View, CA) using a three-plasmid expression system (pLKO.1-shRNAs-puro ...
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bioRxiv - Genetics 2019Quote: Viral RNA was extracted from cell suspensions using a Viral RNA Extraction Kit (TaKaRa) and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: HeLa cells at 40% confluence on coverslips were transfected with pEGFP-N2 (Clontech, USA) constructs encoding variants of BteA-GFP fusion protein (S3 Table ...
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bioRxiv - Molecular Biology 2020Quote: ... These cell lines were tested negative for mycoplasma (TaKaRa PCR Mycoplasma Detection Set; 6601), maintained in RPMI1640 (Nacalai Tesque ...
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bioRxiv - Cell Biology 2019Quote: Total RNA of the cultured cells were harvested with RNAiso Plus reagent (TaKaRa, Japan) following the manufacture’s instruction ...
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bioRxiv - Immunology 2020Quote: ... 106 cells per well were transferred into RetroNectin-coated 24well plates (Takara Bio, USA). Virus supernatant was centrifuged (300xg ...
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bioRxiv - Genomics 2019Quote: ... transduced with concentrated shRNA-expressing lentiviruses harvested from LentiX-293T cells (Takara Bio; 632180), and cultured in BD Matrigel Matrix High Concentration (BD Biosciences ...
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bioRxiv - Genetics 2021Quote: ... HT-29 cells were transduced with the pLVX-Tet3G regulator vector (Takara Bio USA) and a stable Tet-On 3G transactivator-expressing clonal HT-29 cell line (HT-29-pLVX-Tet3G ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated from K562 and GM12878 cells using RNAiso Plus (Takara Bio) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 μl premix WST-1 cell proliferation reagent (Takara Bio Inc, Clontech Laboratories, Inc.) was added to each well ...
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bioRxiv - Cell Biology 2021Quote: The single-cell sequencing library was generated using the ICELL8 cx platform (Takara Bio) and sequencing was performed using the HiSeq 3000 sequencer (Illumina ...
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bioRxiv - Microbiology 2020Quote: Lenti-X 293T™ cells (H. sapiens; sex: female) obtained from Takara (cat. #632180) and Huh-7.5 cells (H ...
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bioRxiv - Microbiology 2020Quote: Phosphatidylserine (PS) externalization was evaluated by staining protoplasted cells with Annexin V-FITC (Takara). Protoplast were generated as previously described (74) ...
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bioRxiv - Immunology 2020Quote: mRNA was extracted from the CD8+ T cells using the TRIzol agents (TAKARA, Japan). mRNA expression was quantified in qRT-PCR using the TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2022Quote: ... triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), trans-plasmid with PHP.B capsid (Ben Deverman ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa-KRAS(G12V) cells were maintained in 10% tetracycline-free FBS (Takara Bio, 631101) and KRAS(G12V ...
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bioRxiv - Immunology 2022Quote: ... Lentivirus containing sgRNA-pLGP was generated by transfecting Lenti-X 293T cells (Takara Bio) with sgRNA-pLGP ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Lentiviral particles were produced by transfecting HEK293T cells (#632180; Clontech, Westburg, Leusden, The Netherlands) according to the manufacturer’s protocol (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from ICP1 cells using RNAiso Plus (Takara Bio, Dalian, China) and was reverse-transcribed to cDNA using HiScript® II Q Select RT SuperMix (Vazyme ...
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bioRxiv - Microbiology 2022Quote: NK cell activity was quantified using the LDH Cytotoxicity Detection Kit (MK401, TaKaRa, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... CD34+ cells were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVO 10 media (Lonza ...
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bioRxiv - Immunology 2023Quote: Immunocult-stimulated human CD3+ T cells were retrovirally transduced on Retronectin-coated plates (TaKaRa). 500 µL of cells per well at 0.5 × 106 cells/mL were supplemented with 0.5 mL retroviral supernatant and centrifuged for 90 minutes at 900 g at 32°C ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant AAV9 vectors were generated by co-transfection of AAVpro 293T cells (Takara Bio) with three plasmids ...
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bioRxiv - Cancer Biology 2023Quote: The total RNA of cells was isolated using RNAiso Plus Regent (Takara, Catalog # 9108) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... HT-1080 (ATCC: CCL-121) and LentiX lentiviral packaging cells (Clontech, cat. no. 632180) were cultivated in DMEM (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with mRNAs or DNAs via liposomes (Invitrogen Lipofectamine or Takara Xfect) or PEI (Polysciences PEImax ...
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bioRxiv - Genomics 2023Quote: ... cells were co-transfected with 1 µg of pEGFP-N1 plasmid (Takara Bio USA). Where indicated ...
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bioRxiv - Cancer Biology 2023Quote: HEK 293T cell line (Lenti-X™ 293T, Takara Bio, San Jose, CA, USA) was cultured in DMEM High Glucose (DMEM-HG cat# SH30243.02 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exchanged with fresh media containing B/B homodimerizer (500nM) (Takara Bio, #635059) and/or Baf-A1 (100nM ...
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bioRxiv - Systems Biology 2023Quote: ... those sgRNA-expressing cell lines were infected with 5x concentrated lentivirus (LentiX Concentrator, Takara) for the same dCas9 fusions ...
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bioRxiv - Genomics 2023Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
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bioRxiv - Cancer Biology 2023Quote: ... cell proliferation was measured using WST-1 Reagent (Takara Bio, Inc., Clontech Laboratories, Inc.) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cell proliferation was measured using WST-1 Reagent (Takara Bio, Inc., Clontech Laboratories, Inc.) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and the excised DNA was purified and transfected into Adeno-X 293 cells (TaKaRa). When late CPE was observed ...
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bioRxiv - Synthetic Biology 2023Quote: Human Embryonic Kidney (HEK) 293 cells (ATCC, CRL-1573) and HEK293T-LentiX (Takara Biosciences) were cultured in standard culture conditions (37ºC and 5% CO2 ...
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bioRxiv - Systems Biology 2023Quote: ... Lentiviruses carrying sgRNAs were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) by transfection and concentrated using Lenti-X concentrator (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... Seventy-percent confluent Lenti-XTM 293T cells (Takara Bio USA, Inc., Mountain View, CA) were transfected with Hdac3 lentiviral plasmids (Hdac3 KO or empty vector control [EV])26 ...
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bioRxiv - Biophysics 2023Quote: ... triple transfection of HEK-293T cells was performed using the pHelper plasmid (TaKaRa, USA), trans-plasmid with either the AAV2/9 ...
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bioRxiv - Bioengineering 2023Quote: Total RNA was isolated from microbial cells using NucleoSpin RNA (Takara Bio, Otsu, Japan). To prepare a complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses were generated by transient co-transfection of Lenti-X 293T cells (Takara, #632180). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted from cells using the NucleoSpin® RNA kit (Cat# 740955.50, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were grown in HEK293T medium except 10% tetracycline-free FBS (Takara Bio) was used ...
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bioRxiv - Immunology 2024Quote: CD8+ T-cells were exposed to the SMARTerTM RACE cDNA Amplification Kit (Clontech/Takara) to identify and annotate ROPN1/B epitope-specific TCRα- and β-chains based on Kunert et al ...
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bioRxiv - Immunology 2024Quote: CD8+ T-cells were exposed to the SMARTerTM RACE cDNA Amplification Kit (Clontech/Takara) to identify and annotate ROPN1/B epitope-specific TCRα- and β-chains based on Kunert et al ...
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bioRxiv - Neuroscience 2023Quote: ... was packaged as follows: 80% confluent Lenti-X™ 293T cells (Takara Bio; #632180) were transfected in 15-cm plates using Lipofectamine 3000 transfection reagent (ThermoFisher ...