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Citations for Takara Bio :
1501 - 1550 of 2318 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Total RNA was isolated from cells with the use of RNAiso plus (Takara), and up to 0.5 µg of the RNA was subjected to RT with the use of ReverTra Ace qPCR RT Master Mix with gDNA remover (Toyobo) ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 h cells were transfected with an eGFP vector (3 μg; Clontech) using Fugene-6 HD reagent according to the manufacturer's protocol ...
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bioRxiv - Cell Biology 2020Quote: ... HPC cells were transfected using Xfect polymer (DSS Takara Bio, New Delhi, India) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Plasmids for lentivirus packaging were amplified by transformation into stellar competent cells (ClonTech) and extracted with endotoxin-free maxi-prep DNA extraction kit (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... was inducibly expressed in Lenti-x-293T human female kidney cells from Takara Bio (Catalog # ...
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bioRxiv - Immunology 2020Quote: ... and TDL (250 cells per subset) were sorted directly into lysis buffer (Takara) using a FACSAriaII (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Lenti-X 293T cells were purchased from Clontech (no. 632180; Mountain View, CA). These cells were maintained in DMEM (no ...
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bioRxiv - Cell Biology 2021Quote: ... The HEK293-based Lenti-X 293T (HEK293T) cell line was obtained from Takara Bio (Shiga ...
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bioRxiv - Molecular Biology 2020Quote: ... Adenoviruses were produced and amplified in Adeno-X(tm) 293 cells (Takara, # 632271), while titter was determined by fluorescence-activated cell sorting.
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bioRxiv - Cancer Biology 2021Quote: Tissues and cells were homogenized in 1 ml RNAisoTM Plus lysis buffer (TAKARA). Total RNA was extracted and 2 μg RNA was transcribed into cDNA with M-MLV reverse transcriptase (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from cells or tissues using RNAiso Plus (Takara Bio) for analysis by qRT-PCR as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... These plasmids were transfected into Foxd3 cKO cells using Xfect transfection reagent (Clontech). The cells were kept under puromycin selection to obtain polyclonal cell lines ...
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bioRxiv - Immunology 2021Quote: 293T Lenti-X human embryonic kidney cell line (Clontech, Mountain View, CA, USA) was used for LV or IDLV production by transient transfection as previously described22,58 ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA from these cells was extracted using RNAiso Plus reagent (Takara, Beijing) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were then transformed into Stellar™ competent cells (Takara Bio; Cat #: 636766), and plasmids were subsequently isolated using Qiagen mini-prep kits (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... cells were transferred to fresh medium containing 10% Tet System Approved FBS (Clontech) and 1 µg/mL puromycin (Sigma ...
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bioRxiv - Immunology 2020Quote: Lenti-X™ 293T cells (Takara Bio, Clontech, Saint Germain en Laye, France) were grown using complete Dulbecco’s Modified Eagle Medium F-12 (DMEM F-12 ...
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bioRxiv - Molecular Biology 2020Quote: ... These constructs were transformed into Y2H Gold cells following the manufacturer’s protocol (Clontech). Self-activation and toxicity detection of the recombinant plasmid and control vectors (pGADT7-T ...
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bioRxiv - Neuroscience 2021Quote: ... The sequence-verified plasmid was transfected into Lenti-X 293T cells (Takara Bio) together with the packaging plasmid psPAX2 (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... where the lysing solution is composed of single-cell lysis buffer (Takara #635013): 1x RNase Inhibitor Murine (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated in media containing 200 μM of A/C Heterodimerizer (Takara) or ethanol control for 4h prior to lysis ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions involving non-AAV plasmids were transformed into Stellar competent cells (Takara Inc.) and those involving AAV plasmids were transformed into NEB Stable competent cells (New England Biolabs).
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bioRxiv - Molecular Biology 2022Quote: For cDNA synthesis the SMART-Seq® Single Cell Kit (634472, Takara BIO) at 1/10 of the original reaction volume was used (down-scaled SMART-Seq® workflow) ...
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bioRxiv - Neuroscience 2022Quote: ... system and the transformation was performed using Stellar competent cells (Takara Bio USA) in heat-shock ...
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bioRxiv - Systems Biology 2024Quote: ... We used 600 µl of cell lysate for total RNA extraction (Clontech 740955), followed by processing for microarray (for interactome assembly ...
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bioRxiv - Genomics 2023Quote: ... For lentiviral transduction the cells were plated on retronectin coated plates (Takara Bio) and transduced in the presence of 8μg/ml protamine sulfate (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: The total RNA of cells was isolated with RNAiso plus (Cat# 9108, Takara). The concentration and purity of RNA samples were measured using a Nanodrop ND-2000 (Thermo Science ...
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bioRxiv - Biochemistry 2023Quote: ... Lenti-X™ 293T cell line (human, female origin) were purchased from TaKaRa and used without further authentication ...
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bioRxiv - Bioengineering 2024Quote: ... lentivirus targeting mCherry to the cell membrane (Takara, 0026VCT, rLV.EF1.mCherry-Mem-9) was used according to manufactures recommendation ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified cell lysate was incubated with pre-equilibrated Ni- NTA resin (Takara) for 2 h at 4°C in slow shaking conditions ...
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bioRxiv - Microbiology 2023Quote: Total RNA of RAW264.7 cells were isolated using the RNAiso plus (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from tissues and cells was extracted using TRIzol reagent (Takara, Japan) and were reversed transcribed into first-strand cDNA using the PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Genomics 2023Quote: ... were described previously.136 Lenti-X 293T cells were purchased from Takara (#632180). K562 CRISPRi and RPE1 CRISPRi cell lines were authenticated by analysis of short tandem repeats as exact matches to the corresponding line ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from cell pellets using Nucleospin RNA Plus XS kit (Takara), and first strand cDNA synthesis was performed using Superscript IV VILO Master Mix with ezDNAse (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... the mRNAs in these cells were extracted using RNAiso Plus (Takara, Cat:9109) and then sequenced by Biomarker Techonologies using Illumina sequencing technology ...
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bioRxiv - Physiology 2023Quote: ... Titering was performed on Ad293 cells using Adeno-X Rapid Titer kit (Clontech) according to the instructions of the manufacturer 28.
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bioRxiv - Neuroscience 2023Quote: ... Cell debris was filtered from the collected medium and Lenti-X Concentrator (Clontech) was then used to precipitate the lentivirus in the medium ...
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bioRxiv - Genomics 2023Quote: ... cells were switched to media made with Tet System Approved FBS (Takara 631367) to reduce background induction ...
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bioRxiv - Microbiology 2023Quote: ... and MS4 (feline B-cell lymphoma) (73) using an RNAiso Plus kit (Takara), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with the Rapalog A/C hetero-dimerizer rapalog (635057, Takara) for 24 h ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: Using the Lenti-X 293 T cell line (Takara Bio Inc., Otsu, Japan) and the pLVSIN-EF1α-AcGFP-C1 vector ...
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bioRxiv - Neuroscience 2024Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), the trans-plasmid providing viral capsid PHP.eB (a generous gift from Viviana Gradinaru ...
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bioRxiv - Neuroscience 2024Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), the trans-plasmid providing viral capsid PHP.eB (a generous gift from Viviana Gradinaru ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from Mm cells using the TRIzol Reagent (Takara, Japan). cDNA was synthesized using a PrimeScript reverse transcriptase reagent kit (Takara ...
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bioRxiv - Neuroscience 2024Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), trans-plasmid providing viral capsid PHP.(e)B (a generous gift from Viviana Gradinaru (Addgene plasmid #103005 ...
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bioRxiv - Neuroscience 2024Quote: ... triple transfection of HEK-293T cells was performed using pHelper plasmid (TaKaRa/Clontech), trans-plasmid providing viral capsid PHP.(e)B (a generous gift from Viviana Gradinaru (Addgene plasmid #103005 ...
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bioRxiv - Biophysics 2024Quote: ... wild-type (WT) MDCK cells were transfected with pAcGFP1-Mem vector (Takara, 632509). To decouple the LINC complex by overexpressing dominant-negative KASH protein ...
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bioRxiv - Biochemistry 2024Quote: ... Transformation was performed with purified PCR products in Stellar Competent Cells (Takara, 636763). Primers used for PCR and sequencing are listed below: