Labshake search
Citations for Takara Bio :
1151 - 1200 of 1758 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... the lentivirus collected from the cell culture media was concentrated using Retro-X concentrator (Takara). The concentrated lentivirus was used immediately to transduce human NSCLC cell lines ...
-
bioRxiv - Genomics 2020Quote: ... in a 1:8 vector:insert optimized ratio and transformed into Stellar competent cells (636766, Clontech) to maximise the number of individual transformants (800,000 individual clones) ...
-
bioRxiv - Neuroscience 2020Quote: ... The LDH level was measured by cell counting using an LDH cytotoxicity detection kit (Takara).
-
bioRxiv - Cell Biology 2021Quote: ... above constructs in retroviral packaging vectors were transfected with pVSVG into GP2-293 cells (Clontech), constructs in lentiviral packaging vectors were transfected with pCMV8.91 and pVSVG into 293T cells ...
-
bioRxiv - Molecular Biology 2020Quote: ... HeLa cells were transfected with the following plasmids: pEGFP as control vector expressing GFP (Clontech), and pEGFP-M27 ...
-
bioRxiv - Immunology 2022Quote: ... 0.5 × 106 cells were plated in a 24-well pre-coated with retronectin (Takara Bio). Virus-containing medium was concentrated by ultracentrifugation and added to cells for 8 h ...
-
bioRxiv - Microbiology 2022Quote: Purified plasmids were transfected into HeLa 229 cells using the Xfect transfection reagent (Takara Biosciences) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Cells were lysed by sonication and then purified via a TALON cobalt affinity resin (Clontech). The resin was washed with 40 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2022Quote: The parental MCF10A cells were stably transduced with tetracycline-OFF-transactivator system (tet-OFF; Clontech) following a previously described protocol (pubmed.ncbi.nlm.nih.gov/27815877/) ...
-
bioRxiv - Cell Biology 2022Quote: ... Lentiviruses containing guide RNAs targeting GORAB were created in HEK293 Lenti-X cells (Takara 31966021) plated on 10 cm dishes the day before transfection ...
-
bioRxiv - Cancer Biology 2022Quote: L3.6 cells were transduced with either an EV pLVX-IRES-Puro (Clontech, Mountain View, CA) or the same backbone vector expressing human K17 cDNA (wild-type or mutations of S10-13A ...
-
bioRxiv - Immunology 2022Quote: Gammaretroviral transduction of γδ T cells was carried out in RetroNectin (Takara Bio, Tokyo, Japan)–coated 24-well plates ...
-
bioRxiv - Immunology 2022Quote: ... cells were transfected with pCMV-VSV-G (1.5 μg) and pRETROX TET3G (2.5 μg) (Clontech) using Lipofectamine 2000 (10 μL) ...
-
bioRxiv - Microbiology 2022Quote: ... Lentiviral plasmids were packaged into pseudoparticles via triple-plasmid co-transfection into HEK293T cells (TaKaRa). pTsin or pWPI plasmids ...
-
bioRxiv - Systems Biology 2022Quote: ... Cells were then treated with 200nM rapalog (A/C heterodimerizer, previously AP21967) (Takara Bio., 635055) and immediately subjected to live cell microscopy with images being taken every 30 minutes for 12 hours ...
-
bioRxiv - Genomics 2022Quote: 0.25-0.5×106 DM Cas9 cells were lentivirally transduced using Retronectin-mediated (Takara Bio #T100A) transduction with pooled guide RNA libraries (Supplemental table 1) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The AAV vector was produced by the transfection of AAVpro293 cells (Takara Bio, Shiga, Japan) with three plasmid transfections (Helper-free system) ...
-
bioRxiv - Molecular Biology 2022Quote: Single-cell K562 libraries were prepared using the SMART-seq Stranded (SSS) Kit (Takara Bio), following the manufacturer instructions with modifications ...
-
bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells or frozen tissues using the TRIzol Reagent (Takara, Japan). The complementary DNA (cDNA ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lentiviral vectors were packaged into lentivirus particles by co-transfecting Lenti-X 293T cells (Clontech) with pMDLg/pRRE (a gift from Didier Trono ...
-
bioRxiv - Microbiology 2022Quote: ... The resulting plasmid was transfected into 293T cells using Lentiviral High Titer Packaging Mix (Takara) to obtain the lentiviral vector for TSG101–venus transduction ...
-
bioRxiv - Cell Biology 2022Quote: ... the cells were treated with 200 ng ml−1 of Dox (#631311; TaKaRa, Kusatsu, Japan) for 24 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were washed twice in DMEM containing 0.001 U/µL DNase I (Takara, 2270A) and 5% FBS ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were pre-treated 1 hour prior to the assay with A/C Heterodimerizer (Takara) diluted at 1:200 in complete media concurrent with JF-Halo or -SNAP dye labeling ...
-
bioRxiv - Systems Biology 2023Quote: ... The cells containing the Cas12-pRG232 insertion were selected with 0.5ug/mL puromycin (Takara, 631305) for three days ...
-
bioRxiv - Immunology 2023Quote: ... inducible cell lines were generated using the Tet-On Advanced Inducible Expression System (Clontech Laboratories) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... 661W cells were transfected with the calcium phosphate technique or the XfectTM transfection reagent (Takara). MEF cells were transfected using TurboFectTM Transfection Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... The ligation reaction was purified by ethanol precipitation and transformed into Stellar Competent Cells (Clontech) to assess library diversity by Sanger sequencing of 10 clones ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were cultured using Cellartis DEF-CS 500 culture system (Takara Bio, cat. no. Y30012).
-
bioRxiv - Systems Biology 2023Quote: ... Ligated plasmid products were transformed into stellar competent cells (E. coli HST08 strain, Takara Bio). See Table S1 for full descriptions of constructs.
-
bioRxiv - Developmental Biology 2023Quote: ... the RUES2 cells were thawed and plated on culture plates coated with iMatrix-511 (Takara) in StemFit Basic04 Complete medium (Amsbio) ...
-
bioRxiv - Cell Biology 2023Quote: Lentivirus was generated in a 10cm dish format by transfecting HEK293T Lenti-X cells (Takara) at roughly 80% confluency with 4.5μg of an equimolar mixture of three plasmids encoding the envelope and viral packaging components from the laboratory of Didier Trono (pMD2.G ...
-
bioRxiv - Cancer Biology 2023Quote: ... The TOB1-AS1 overexpression plasmid was amplified by transforming Stellar™ Competent Cells (Takara, 636763) with the plasmid as per instructions and isolated by QIAGEN HiSpeed Plasmid Midi Kit (QIAGEN ...
-
bioRxiv - Immunology 2023Quote: ... Full-length cDNA amplification of single cells was performed per the kit protocol (Takara Bio). The analysis of the surface markers of the positive cells was performed on FlowJo.
-
bioRxiv - Cell Biology 2023Quote: HaCaT cells were treated with the E-cadherin-blocking antibody (SHE78-7, Takara, Shiga, Japan), PY-60 (Axon Medchem ...
-
bioRxiv - Cell Biology 2023Quote: ... 1.6 µg of a transfer plasmid was transfected into LentiX 293T cells (Clontech Laboratories, inc.) with 0.8 µg of psPAX2 (addgene #12260 ...
-
bioRxiv - Microbiology 2024Quote: ... The recombinant construct was transformed into Escherichia coli StellarTM Competent Cells (dam–/dcm–) (Takara Bio) according to the supplier’s protocol (ClonTech ...
-
bioRxiv - Immunology 2024Quote: ... and pMD2-VSV-G to HEK293T cells using the CalPhos Mammalian Transfection Kit (Clontech Laboratories). Viral supernatants were filtered through 0.45 μm low-protein-binding filters ...
-
Exploratory mass cytometry analysis reveals immunophenotypes of cancer treatment-related pneumonitisbioRxiv - Immunology 2023Quote: ... The resultant cell pellets were then resuspended in Cellbanker 1 cryopreservation solution (Takara, catalog #210409). This suspension was aliquoted into cryovials and gradually frozen to –80°C and stored at –80°C until required for experimental analysis ...
-
bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from tissues or cells using RNAiso Plus (Takara Biotechnology, Japan; 9109) according to the manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2024Quote: Following plasmids were used in this assay to generate stable cell lines: pCMV-HRasG12V (Clontech), Piggyback vectors ...
-
bioRxiv - Cancer Biology 2024Quote: 1 x 106 ACKP cells were transfected with 3 µg pE2F-TA-luc plasmid (Takara) and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega ...
-
bioRxiv - Bioengineering 2024Quote: ... and the receptor- or payload-encoding viral expression plasmids (2.0 μg) into Lx293T cells (Clontech) in 6-well plates with Lipofectamine 3000 (Thermo Fisher L3000001) ...
-
bioRxiv - Plant Biology 2023Quote: ... One µL of the reaction medium was then used to transform StellarTM competent cells (Clontech). DNA plasmids were sequenced using the service of Eurofins Genomics (Germany).
-
bioRxiv - Developmental Biology 2021Quote: ... at a density of 0.8× 104 cells per cm2 in NDiff227 media (Takara cat. no. Y40002) supplemented with 1% KSR (Gibco cat ...
-
bioRxiv - Molecular Biology 2019Quote: ... These overlapped fragments were then cloned into competent cells with standard In-Fusion HD Cloning (TaKaRa) techniques ...
-
bioRxiv - Biophysics 2019Quote: ... Cells were harvested and purified using TALON His-Tag Purification protocol (Clontech Laboratories, Mountain View, CA.) The SUMO tag was cleaved by Ulp-1 as described above and the protein was further purified using ion-exchange chromatography (Bio-Rad ...
-
bioRxiv - Cancer Biology 2019Quote: ... The human patient PDX cell lines were maintained in RPMI-1640 medium containing 10% FBS (Clontech). KRAS mutation status ...
-
bioRxiv - Cell Biology 2019Quote: ... cloned in pRACE vector as per kit instructions and transformed into Stellar component cells (Clontech, 636763). The inserts were verified by Sanger sequencing ...
-
bioRxiv - Synthetic Biology 2021Quote: ... but before cell application lentivirus was concentrated using the Lenti-X Concentrator kit (Takara Bio #631232) according to manufacturer’s instructions ...