Labshake search
Citations for Takara Bio :
1351 - 1400 of 1758 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Cells were sorted directly into buffer for SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Subsequent processing was carried out by the UCL Genomics sequencing facility ...
-
bioRxiv - Neuroscience 2023Quote: All cells were cultured in high glucose DMEM with 10% Fetal Bovine Serum (tetracycline free, Clontech or Gibco) and penicillin/streptomycin 50units/ml-50 mg/ml ...
-
bioRxiv - Neuroscience 2022Quote: As previously described (Lester et al., 2021), H4 cells (ATCC Cat# HTB-148, RRID:CVCL_1239) stably expressing the pIRESpuro3 vector (Clontech) containing a codon-optimized 0N4R MAPT gene with the P301S point mutation and tagged with YFP were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Cancer Biology 2022Quote: CPT1a knockdown cell lines were generated using the shRNA-expressing lentiviral pLKO-shRNA2 vector (No. PT4052-5; Clontech), with a puromycin selection cassette ...
-
bioRxiv - Cancer Biology 2022Quote: ... Surviving cells were then infected with lentivirus for either mitoGFP or NDI1 expressed in the pLVX-TRE3G (Clontech) response vector and selected with puromycin (2 μg/mL).
-
bioRxiv - Cell Biology 2022Quote: Nuclei were isolated from 1.5 × 109 DT40 cells and digested with 60 units/ml MNase (Takara Bio Inc.) in buffer A (15 mM Hepes-KOH ...
-
bioRxiv - Bioengineering 2023Quote: ... T cells were mixed with lentivirus at multiplicity of infection (MOI) equal to 5 in Retronectin (Takara, T100B)-coated culture plates and centrifuged at 1800 g for 1 h at 32 °C for lentiviral transduction before returning to normal culture condition ...
-
bioRxiv - Molecular Biology 2023Quote: RNA extracion from the cells and mouse hearts was performed using the sophisticated Trizol reagent (Takara, Kyoto, Japan). Subsequently ...
-
bioRxiv - Neuroscience 2023Quote: ... by dispensing individual cells in 5 nL drops directly into 3 µL ice-cold single cell lysis buffer (scLB, 0.134% Triton X-100 [Sigma], 0.5 U/µL recombinant RNase inhibitor [Takara, 2313B] ...
-
bioRxiv - Cell Biology 2023Quote: ... Cre recombinase proteins were delivered into TP53-missense mutation knock-in cells using Cre Recombinase Gesicles (Takara Bio).
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then induced for the indicated amount of time with 1 μM Shield-1 ligand (Takara bio) to stabilize the TRF1-FokI protein and 1 μM 4-OHT (Cayman Chemical ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were isolated with LCM in 6.4 μL of cell lysis buffer (0.25 μL of 40 U/μL Takara RNase Inhibitor ...
-
bioRxiv - Systems Biology 2023Quote: ... This HEK293T reporter line was generated by transducing cells with lentivirus containing EGFP expressed from a pTRE3G (Clontech) promoter ...
-
bioRxiv - Genomics 2023Quote: ... Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million 797 cells with homozygous insertions were infected with 30ul of each adeno-associated CRE virus (AAV2) ...
-
bioRxiv - Developmental Biology 2024Quote: Single-cell cDNA synthesis was performed using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) according to the manufacturer’s protocol with some modifications as previously described17 ...
-
bioRxiv - Biochemistry 2023Quote: ... Genomic DNA from WT and Strt1−/− cells was extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using specific primers (Oligonucleotide sets 3 ...
-
bioRxiv - Developmental Biology 2023Quote: ... 18-20 μg of indicated plasmids were transfected in HEK293T cells using the CalPhos Mammalian Transfection Kit (Takara). After 48h ...
-
bioRxiv - Cancer Biology 2024Quote: ... Retroviral transduction was achieved by spinoculation of 3 × 106 mouse T cells on retronectin-coated plates (Takara Bio) with neat retroviral supernatant harvested from 293T packaging cells (2000xg ...
-
bioRxiv - Neuroscience 2023Quote: ... All cell lines were tested for mycoplasma contamination regularly with PCR Mycoplasma Detection Set (TaKaRa, Cat. no. 6601) and maintained under passage 20 ...
-
bioRxiv - Bioengineering 2024Quote: ... T cells were transduced with the above-mentioned γ-retroviral SFG construct following plating on retronectin (Takara Bio)-coated plates and spinoculation ...
-
bioRxiv - Neuroscience 2024Quote: pCAG-XCaMP-R63 (gift from Haruhiko Bito, University of Tokyo) was transformed using Stellar Competent Cells (Takara Bio.), grown in antibiotic-treated LB broth at 37° C ...
-
bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was purified from at least 100 million cells of each sample using NucleoSpin Blood XL (Takara) or QIAamp DNA Blood Midi Kit (QIAGEN) ...
-
bioRxiv - Cell Biology 2024Quote: ... Lentiviruses were produced by transfecting 293T cells with the pLVX constructs together with packaging and envelope vectors (Clontech) using the calcium phosphate precipitation method ...
-
bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from the total RNA of HapT1 cells using the PrimeScript RT Reagent Kit (Takara Bio). The amounts of Pts and Actb were quantified on a LightCycler 480 (Roche ...
-
bioRxiv - Immunology 2024Quote: ... A stable IFN-λ-expressing cell line was obtained by transfection of one million Lenti-X (HEK293T, Takara) cells with 500 ng Super PiggyBac Transposase vector and 1250 ng of PiggyBac transposase mammalian expression vector using Lipofectamine 3000 following to the manufactureŕs instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from drug/vehicle-treated cell lines using NucleoSpin RNA kit (Takara, Catalog no. 740955.50). 2 ug of total RNA was used to synthesize cDNA with qScript Ultra Supermix (Quantabio ...
-
bioRxiv - Cell Biology 2020Quote: ... The emerin and LEM2 coding regions were amplified from HeLa cells derived cDNA and cloned into pEGFP-N3 (Clontech). pEGFP-N3-derived plasmids encoding for LAP2β (Ungricht et al. ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were infected with concentrated lentivirus for 24 h in wells pre-coated with Retronectin (0.2 mg/ml; Takara) in expansion medium supplemented with protamine sulfate (10 μg/ml) ...
-
bioRxiv - Immunology 2021Quote: ... Such isolated naïve CD8 T cells were cultured for 3d prior transducton with 3rd generation lentiviral vectors on RetroNectin (Takara) coated cell culture plates (according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein expression was conducted in BL21(DE3)pLysS cells after 0.3 mM IPTG induction and the complex was purified over Talon (Clontech) cobalt affinity resin as described previously(65 ...
-
bioRxiv - Genomics 2020Quote: ... using a Multi Sample Nano Dispenser (MSND, SMARTer™ ICELL8® Single-Cell System, Takara Bio USA, CA, USA). Chip wells were sealed using SmartChip Optical Imaging Film (Takara Bio USA ...
-
bioRxiv - Genomics 2020Quote: RPE1 cells co-expressing 53BP1-eGFP and Lifeact-RFP were generated using two individual pIRES-Neo3 vector systems (Clontech) harboring either the coding sequence of 53BP1 (representing amino acid 1220-1711 ...
-
bioRxiv - Molecular Biology 2021Quote: 20 cm plates of HEK293T cells were transfected with 10 µg of each plasmid using CalPhos transfection kit (Takara) according to manufacturer’s instructions and harvested 3 days later ...
-
bioRxiv - Immunology 2021Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA).
-
bioRxiv - Bioengineering 2021Quote: ... coli BL21 (DE3) Gold cells harboring the corresponding expression plasmid and the TaKaRa chaperone-plasmid pTF16 (TAKARA BIO INC.) were used to inoculate 500 mL of autoinduction medium (464 mL LB medium ...
-
bioRxiv - Molecular Biology 2021Quote: DIE cells were cultured in growth medium consisting of IMDM basal medium with 10% Tet system-approved FBS (Clontech) and 55μM 2-mercaptoethanol ...
-
bioRxiv - Cancer Biology 2020Quote: ... 105 tumor cells in 6-well culture vessels were transfected with 5 μg DNA using XFect (Takara, Kusatsu, Japan) and clones were selected by puromycin (2-10 μg/mL).
-
bioRxiv - Microbiology 2020Quote: ... cells were cultivated for three days in growth medium supplemented with 20 μg/ml of puromycin (Takara Bio Inc.) for PVR1 KO or 40 μg/ml for PVR2 KO ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were originally obtained from the ATCC and routinely tested negative for mycoplasma contamination (PCR Mycoplasma Detection Set, Takara).
-
bioRxiv - Microbiology 2020Quote: ... lysed by sonication and the recombinant His-tagged protein purified from cell-free extracts using IMAC (Talon resin, Clontech) as previously described19.
-
bioRxiv - Plant Biology 2021Quote: ... Each individual effector-HMA combination was then co-transformed into competent Saccharomyces cerevisiae Y2H Gold cells (Takara Bio USA) and selected on SD-Leu-Trp (synthetic dropout ...
-
bioRxiv - Cell Biology 2021Quote: WIPI3-HA construct used for expression in mammalian cells (pJC248): a gBlock (IDT) encoding WIPI3-HA was assembled by Gibson cloning with PCR amplified pcDNA3.1+ (Clontech). ss-HA-CPD in pEGFP_N1 (pJC338) ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were washed twice with 20 mL of D-PBS (Nacalai Tesque) and resuspended in CELLBANKER 1 (Takara Bio) at 1 × 107 cells/mL ...
-
bioRxiv - Bioengineering 2022Quote: HEK293T cells were harvested for RNA extraction using RNeasy Plus Mini Kit (Qiagene) or NucleoSpin RNA Plus Kit (Takara). RNA concentration was measured by nanodrop and 500ng to 1000ng total RNAs were used for reverse transcription in 10 ul reaction volume by High-Capacity RNA-to-cDNA kit (ThermoFisher) ...
-
bioRxiv - Biophysics 2022Quote: Experiments were performed using PtK2 GFP-α-tubulin cells (stable line expressing human α-tubulin in pEGFP-C1, Clontech Laboratories ...
-
bioRxiv - Neuroscience 2022Quote: ... coli cells induced with 100 mM IPTG and purified from bacterial extracts using TALON beads (Takara Bio, Catalog # 635502) according to the vendor’s instructions.
-
bioRxiv - Bioengineering 2022Quote: HEK293T cells were transfected with given plasmids and then harvested for RNA extraction by NucleoSpin RNA Plus Kit (Takara) 48 hour after transfection ...
-
bioRxiv - Immunology 2022Quote: The total RNA of the cell samples was extracted with 1000-800 µl RNAiso Plus reagent (Takara, Kyoto, Japan) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: About 130 ml of clarified cell suspension was combined with 3 mL of TALON® resin (Takara Bio 635503) previously equilibrated in lysis buffer and rocked at 4°C for 1 h ...
-
bioRxiv - Biochemistry 2022Quote: Expression inserts for cell lines were generated by PCR amplification and ligated into the pLVX-tight-Puro vector (Clontech) using the NEB HiFi DNA Assembly Kit (New England Biolabs ...