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Citations for Takara Bio :
1251 - 1300 of 1758 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... we used In-Fusion HD enzyme premix and for bacterial transformation Stellar Chemically competent cells (Clontech, USA). The mutagenic libraries were produced in the 96-well plate with 46 bacterial colonies selected for each (two libraries per 96-well plate ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from HCT-116 cells by using an RNAiso plus reagent (Takara Bio, Japan) and was subsequently subjected to reverse transcription followed by PCR using a OneStep RT-PCR Kit (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... Activated NK cells were transduced with retroviral supernatants on day +4 in human fibronectin-coated plates (Clontech Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... at a ratio of 2.5:1.0:0.6:0.5 to Lenti-X 293T cells (Takara, 632180, Kusatsu, Japan). A transfection reagent ...
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bioRxiv - Biochemistry 2020Quote: ... and cell toxicity was evaluated using the LDH Cytotoxicity Detection Kit (Cat #MK401; Takara Bio, Shiga, Japan).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3) cells containing chaperone plasmid pG-KJE8 (TAKARA, 3340). When the optical density at 600 nm reached 0.6 ...
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bioRxiv - Neuroscience 2020Quote: Cell line CUT&RUN libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (Takara Bio R400676), with the following PCR conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... Complex formation was induced in these cells by treating with AP21967 (A/C ligand heterodimerizer; Takara Bio). MDA-MB-231-iDimerize-c-Met-β1/luc cells were created by lentiviral transduction of MDA-MB-231-iDimerize-c-Met-β1 cells and subsequent confirmation of bioluminescence ...
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bioRxiv - Cell Biology 2021Quote: ... Fibroblast cultures at passage 3 were cryopreserved by suspending cells in CELLBANKER 1 (Takara Bio, Shiga, Japan), slowly cooled to -80 °C using a Mr ...
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bioRxiv - Biochemistry 2022Quote: ... The pTRE3G vector containing PFK1-mEGFP was then co-transfected into HeLa Tet-On 3G cells (Clontech) along with a linear selection marker of hygromycin using Xfect (Clontech) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with the appropriate chemicals at the following concentrations: 1 µg/ml doxycycline (Clontech, 631311), 2.5 µM MG132 (APExBIO ...
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bioRxiv - Cell Biology 2022Quote: ... Immortalization of the cells was achieved by lipofection of the pCDNA-3xHA-hTERT plasmid with Xfect (Takara BIO INC ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets was collected and isolated for total RNA with MiniBEST Universal RNA Extraction Kit (Takara, #9767). Total RNA (1 μg ...
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bioRxiv - Neuroscience 2020Quote: ... Viruses were generated by transient transfection in HEK cells with the vectors psPAX2 and pMD2.G (Clontech) using the calcium phosphate method ...
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bioRxiv - Cancer Biology 2020Quote: ... GFP positive cells were selected by FACS sorting after tetracycline (Takara Bio Inc., San Francisco, CA, USA) treatment (1µg/ml) ...
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bioRxiv - Microbiology 2021Quote: U937 cell lines stably expressing red fluorescent protein membrane were generated using pDsRed-Monomer-Mem (Takara Bio) cloned into pLB vector from Addgene (Addgene plasmid 11619 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was used to transfect cells at 70% confluency with 20 ng of CRE-SEAP reporter plasmid (Clontech) and 20 ng of receptor or empty vector pcDNA-Zeo-tetO DNA per well ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses corresponding to different CAR constructs were transduced into activated T cells in retronectin-coated plates (Takara). Cultures were allowed to expand for 10 days in their media supplemented with 300 IU ml-1 IL-2 ...
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bioRxiv - Molecular Biology 2022Quote: Single cells were prepared using the STORM-seq protocol (referencing the SMART-Seq Stranded Kit – Takara Bio) on the SPT Labtech Mosquito (HV) ...
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bioRxiv - Cancer Biology 2022Quote: KPC cells were transduced with either an empty vector (EV) pLVX-IRES-mCherry (Clontech, Mountain View, CA) or the same backbone vector expressing the human K17 open reading frame cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... All of the cells integrated with rtTA were cultured in Tet Approved FBS and medium from Clontech. All the cells were authenticated by examination of morphology and growth characteristics and confirmed to be mycoplasma-free.
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bioRxiv - Molecular Biology 2019Quote: ... and yeast cells were grown on a minimal medium/-Leu/-Trp according to the manufacturer’s instructions (Clontech). Transformed colonies were plated onto a minimal medium/-Leu/-Trp/-His/-Ade to test for possible interactions.
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bioRxiv - Neuroscience 2019Quote: The overexpression experiments in HEK-293T cells were performed using the following constructs: C2-eGFP-hDNMT3A (Clontech, subcloned from pcDNA3/Myc-DNMT3A1 ...
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bioRxiv - Neuroscience 2019Quote: ... Lysing of cells and particle purification were carried out using AAVpro® Purification Kit (All Serotypes, TAKARA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Gryphon-Ampho packaging cells were transiently transfected with Am-Cyan-Geminin (pRetroX-SG2M-Cyan Vector from Takara), pLZRS or pLZRS.BCLxL using Lipofectamine 2000 transfection reagent (Invitrogen ...
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Maternal transmission of a paramutant phenotype requires intact DNMT2 functions in the male germlinebioRxiv - Genetics 2020Quote: ... The single cell cDNA libraries were made using SMART-Seq v4 Ultra Low Input RNA Kit (Takara). The cDNAs were fragmentized and Illumina Sequencing adaptors were added using Nextera DNA Library Preparation Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... AAVs particles were then purify from AAV-producing cells using AAVpro Purification Kit Maxi (Takara, Cat. #6666).
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bioRxiv - Developmental Biology 2021Quote: ... RNA were extracted from each well and converted into cDNA using SAMRT-Seq Single cell kit (Takara). DNA library was made and sequenced at Single Cell Genomic Core at the Baylor College of Medicine ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% Tet System Approved FBS (Clontech), 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco ...
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bioRxiv - Physiology 2021Quote: ... Adenovirus was generated in HEK293A cells and titered using the Adeno-X Rapid Titer Kit (Takara Bio). For differentiation ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was collected from MCF7-Vector and MCF7-YAP1-S127A cells using TRIzol (Takara, Cat. #9108) reagent according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells and purified to near-homogeneity by metal-affinity chromatography using TALON resin (Takara Bio, USA). After purification ...
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bioRxiv - Immunology 2021Quote: ... A2058 and MDST8 as previously described (7) with small changes for adherent cell lines omitting RetroNectin (Clontech). LCL-1 was transduced with single minigenes ...
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bioRxiv - Immunology 2020Quote: TZM-bl (kindly provided by Dr. Quentin Sattentau, University of Oxford) and Lenti-XTM 293T cells (Takara Bio ...
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bioRxiv - Immunology 2021Quote: ... Supernatant from Expi293 transfected cells was manually purified using TALON metal affinity chromatography (Takara bio Inc – 635502), according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... gDNA from all cells were isolated using the Machery Nagel L Midi NucleoSpin Blood Kit (Clontech, 740954.20). Modifications to the manufacturer’s instructions were added as follows ...
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bioRxiv - Cell Biology 2022Quote: Transient transfections for all the cell lines were performed using Xfect transfection reagent from Takara (Cat# 631318) in 6 well plates (Falcon ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell survival was then quantified versus a DMSO/vehicle control by WST-1 (Takara Bio, Catalog #MK400) or AlamarBlue (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... the CPER products were transfected into HEK293-hACE2/hTMPRSS2 cells by using TransIT-LT1 (Takara, Cat# MIR2305). At one day post-transfection ...
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bioRxiv - Genomics 2022Quote: ... 10ul of the reaction mix was transformed directly into 100ul of Stellar chemically competent cells (Takara Bio), cells were allowed to recover at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Large-scale production of high-titer lentivirus was generated using Lenti-X™ 293T Cells (TaKaRa, #632180) according to an established ...
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bioRxiv - Cancer Biology 2023Quote: ... bacteria cells were pelleted and BAC DNA was extracted using the NucleoBond Xtra BAC kit (Takara, 740436) as per manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HepG2 cells were resuspended in ice-cold PBS supplemented with protease inhibitors (Clontech, Mountain View, CA, USA) and lysed in ice bath by sonication in cycles of 10 s on /5 s off for 1 min at 6–10 μm amplitude at 25 % intensity from an exponential ultrasonic horn of 3 mm in a Soniprep 150 MSE (MSE Ltd. ...
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bioRxiv - Microbiology 2023Quote: ... The CPER products (25 µl) were transfected into VeroE6/TMPRSS2 cells with TransIT-X2 (Takara, Cat# MIR6003) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: Arc DNA plasmids were transfected into the donor cell culture via PEI or Xfect transfection reagent (Takara) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... applying their current hepatocyte differentiation protocol (Cellartis iPS Cell to Hepatocyte Differentiation System, Cat. No. Y30055, Takara Bio Europe AB ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells were transiently transfected with the expression control vector pCMV-TRE3G (Clontech, Mountain View, CA, USA) and the coexpression construct pTRE3G-BI/GPR83-LgBiT:SmBiT-ARRB2 that encodes a C-terminally LgBiT-fused human GPR83 (LgBiT-GPR83 ...
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bioRxiv - Cancer Biology 2023Quote: ... CT-2AZsGreen cells were generated using lentiviral particles derived from a pLVX-ZsGreen1C1 vector (Takara Bio #632566). All cell lines were grown in DMEM high glucose with 10% FBS (Gibco #16140-071 ...
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bioRxiv - Genetics 2023Quote: ... Plasmids that contain each sgRNA were transfected to HEK293T cells using Lenti-X packaging single shots (Takara) for viral packaging ...
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bioRxiv - Immunology 2024Quote: ... HEK 293T cells were sourced from the American Type Culture Collection (ATCC) and GP2-293 from Takara Bio ...