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Citations for Takara Bio :
1001 - 1050 of 1868 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Lenti-X 293T cells were purchased from Clontech (no. 632180, Mountain View, CA). These cells were maintained in D-MEM (no ...
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bioRxiv - Cell Biology 2021Quote: ... was stably introduced into ChoK1 cells with a pLVX-shRNA1 lentiviral system (Clontech) and selected for with 5 μg/mL of puromycin ...
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bioRxiv - Neuroscience 2021Quote: ... The assembly mixture was transformed into competent DH5α or Stellar cells (Takara Bio) and transformants were screened in a colony PCR for inserts of the correct size using GoTaq polymerase (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... The backbone plasmid used to transfect cell lines was pIRES2-EGFP2 (Clontech, UK). Coverslips were fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were sorted into 4µl lysis buffer with 0.1µl RNase inhibitor (Clontech), 1.9µl 0.4% Triton X-100 ...
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bioRxiv - Developmental Biology 2020Quote: The HEK293T cell line used for lentivirus packaging was obtained from Takara (632273). The DLD-1 cell line was obtained from ATCC (CCL-221) ...
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bioRxiv - Genomics 2019Quote: ... Retroviruses were packed using the EcoPack 2-293 cells (Clontech, Mountain View, CA) and infections were performed as described12 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by transfection into Escherichia coli DH5α competent cells (Takara Bio, Shiga, Japan). Positive clones were identified by blue-white screening and at least three positive clones were sequenced ...
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bioRxiv - Immunology 2021Quote: ... cell death was assayed using a lactate dehydrogenase release assay kit (Takara BioProduct) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: CRT-null HEK293T cells were generated using Guide-it CRISPR/Cas9 System (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Retroviral production was performed by transient transfection of HEK-GP2 293 cells (Clontech) with 0.8 μg pCMVsVg and 1.2 μg of pLPC-puro-N-Flag-NELFE plasmids using JetPrime reagent (polyplus) ...
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bioRxiv - Biochemistry 2021Quote: ... viral particles were first generated in HEK-293FT cells using PVLX-puro (Clontech) alongside psPAX2 and pMD2.G obtained from Addgene ...
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bioRxiv - Biochemistry 2020Quote: Constructs for mammalian cell experiments were cloned using backbone PCR and inFusion (Clontech). Monobody HA4 or OptoMB variants were PCR amplified and Gibson-assembled from bacterial plasmids (described above ...
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bioRxiv - Cell Biology 2021Quote: The Lenti-X 293T Cell Line (Takara Bio USA, Inc., Mountain View, CA) was cultured according to the manufacturer’s instructions and transfected with 5 μg pLenti-C-mGFP-P2A-Puro (control ...
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bioRxiv - Microbiology 2021Quote: The human kidney epithelial cell line Lenti-X 293T was purchased from Takara. The human liver cell line Huh7.5.1 was provided by Dr ...
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bioRxiv - Microbiology 2021Quote: ... This transfection mix was added to 1.5×106 Lenti-X 293T cells (Takara Bio #632180 ...
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bioRxiv - Microbiology 2019Quote: ... Infected cells and uninfected controls were sorted directly into RNA lysis buffer (Clontech) using the BD FACSAria II cell sorter (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from A431 cells using NucleoSpin RNA Plus (Takara Bio), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Single cells were dispensed to a 5184-well microchip using ICELL8 (Takara Bio) system (22 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from tissues and cells with TRIzol reagent (TaKaRa, Japan), and genomic DNA was isolated using a TIANamp Genomic DNA Kit (TianGen Biotech ...
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bioRxiv - Biophysics 2022Quote: ... The lentivirus is generated by transfection of Lenti-X 293T cells (Takara, #632180) with pBOB-EF1-FastFUCCI-Puro (Addgene ...
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bioRxiv - Microbiology 2020Quote: ... Diploid cells were selected with SD supplemented with -Leu/-Trp DO supplement (Clontech) (SD-Leu-Trp) ...
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bioRxiv - Immunology 2020Quote: The human epithelium kidney 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) were maintained in Dulbecco’s Modified Eagles medium (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant plasmid was transformed into Stellar™ Competent Cells (Clontech, Catalog # 636763) according to the manufacturer’s protocol and positive colonies were selected on LB plates containing kanamycin (50 μg/mL).
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bioRxiv - Microbiology 2020Quote: ... was inducibly expressed in Lenti-x-293T human female kidney cells from Takara Bio (Catalog # ...
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bioRxiv - Cell Biology 2021Quote: Lentiviruses carrying genes of interest were generated in HEK293T(Lenti-X) cells (Takara) using Lentiviral High Titer Packaging Mix (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA was transfected into A549 cells by Xfect transfection reagent (Takara Bio), while transfection of ON-Targetplus siRNAs (Dharmacon ...
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bioRxiv - Cancer Biology 2020Quote: ... Cellular proliferation was measured with a WST–1 cell proliferation assay system (Takara). WST–1 reagent was added to each well and incubated for 2 hours at 37 °C ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from cells using the NucleoSpin RNA Kit (Takara Bio) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected using a lentiviral vector containing tdTomato (rLV. EF1. tdTomato9, Clontech), according to the manufacturer’s guidelines ...
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bioRxiv - Genetics 2019Quote: ... HEK293T cells were transfected with wildtype or mutant SCN5A pIRES2-EGFP plasmids (Clontech) using FuGENE 6 (Promega ...
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bioRxiv - Genomics 2022Quote: ... rAAV-9 serotype virons were produced by transfecting AAVpro 293T cell (Takara, 6322723) with pCMV-sadCas9-KRAB (Addgene ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was isolated from cells with the use of RNAiso plus (Takara), and up to 0.5 µg of the RNA was subjected to RT with the use of ReverTra Ace qPCR RT Master Mix with gDNA remover (Toyobo) ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 h cells were transfected with an eGFP vector (3 μg; Clontech) using Fugene-6 HD reagent according to the manufacturer's protocol ...
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bioRxiv - Cell Biology 2020Quote: ... HPC cells were transfected using Xfect polymer (DSS Takara Bio, New Delhi, India) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Plasmids for lentivirus packaging were amplified by transformation into stellar competent cells (ClonTech) and extracted with endotoxin-free maxi-prep DNA extraction kit (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... was inducibly expressed in Lenti-x-293T human female kidney cells from Takara Bio (Catalog # ...
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bioRxiv - Immunology 2020Quote: ... and TDL (250 cells per subset) were sorted directly into lysis buffer (Takara) using a FACSAriaII (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Lenti-X 293T cells were purchased from Clontech (no. 632180; Mountain View, CA). These cells were maintained in DMEM (no ...
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bioRxiv - Cell Biology 2021Quote: ... The HEK293-based Lenti-X 293T (HEK293T) cell line was obtained from Takara Bio (Shiga ...
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bioRxiv - Molecular Biology 2020Quote: ... Adenoviruses were produced and amplified in Adeno-X(tm) 293 cells (Takara, # 632271), while titter was determined by fluorescence-activated cell sorting.
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bioRxiv - Cancer Biology 2021Quote: Tissues and cells were homogenized in 1 ml RNAisoTM Plus lysis buffer (TAKARA). Total RNA was extracted and 2 μg RNA was transcribed into cDNA with M-MLV reverse transcriptase (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from cells or tissues using RNAiso Plus (Takara Bio) for analysis by qRT-PCR as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... These plasmids were transfected into Foxd3 cKO cells using Xfect transfection reagent (Clontech). The cells were kept under puromycin selection to obtain polyclonal cell lines ...
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bioRxiv - Immunology 2021Quote: 293T Lenti-X human embryonic kidney cell line (Clontech, Mountain View, CA, USA) was used for LV or IDLV production by transient transfection as previously described22,58 ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA from these cells was extracted using RNAiso Plus reagent (Takara, Beijing) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were then transformed into Stellar™ competent cells (Takara Bio; Cat #: 636766), and plasmids were subsequently isolated using Qiagen mini-prep kits (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... cells were transferred to fresh medium containing 10% Tet System Approved FBS (Clontech) and 1 µg/mL puromycin (Sigma ...
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bioRxiv - Immunology 2020Quote: Lenti-X™ 293T cells (Takara Bio, Clontech, Saint Germain en Laye, France) were grown using complete Dulbecco’s Modified Eagle Medium F-12 (DMEM F-12 ...
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bioRxiv - Molecular Biology 2020Quote: ... These constructs were transformed into Y2H Gold cells following the manufacturer’s protocol (Clontech). Self-activation and toxicity detection of the recombinant plasmid and control vectors (pGADT7-T ...