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Citations for Takara Bio :
1001 - 1050 of 2435 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the 5′-terminus of the viral genome was determined by 5′/3′ RACE kits (TaKaRa). The resulting whole genome sequence of the GX_P2V variant was deposited in GenBank (accession number MW532698) ...
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bioRxiv - Microbiology 2020Quote: ... 5’ ends of the viral genome were analyzed by 5’-Full RACE Core Set (TaKaRa) and the PCR products of 5’ RACE were cloned using pGEM®-T Easy Vector Systems (Promega ...
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bioRxiv - Genetics 2022Quote: The 5’end of the cloned fragment was amplified with a 5’RACE kit (Takara). The 5’RACE adaptor in the kit was used to evaluate the mRNA ...
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bioRxiv - Biochemistry 2019Quote: ... Protein purity and concentration were determined by SDS-PAGE and BCA protein assay kit (Takara) respectively ...
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bioRxiv - Biophysics 2019Quote: ... cDNAs encoding cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) were purchased from Clontech Com ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was quantified using the TAKARA BCA Protein Assay Kit (TAKARA Bio Inc., Japan). Equal amounts of protein were separated by SDS-PAGE on 10% gels ...
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bioRxiv - Cancer Biology 2021Quote: ... The viral supernatant was then concentrated 20-fold with Lenti-X Concentrator (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the crude viral solution was concentrated using Lenti-x concentrator (Takara, cat.631232) to a final concentration of 108-109 IFU/ml using the Lenti-x qRT-PCR Titration Kit (Takara ...
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bioRxiv - Cell Biology 2019Quote: ... Lenti-X 293T cells were purchased from Clontech (no. 632180, Mountain View, CA). These cells were maintained in D-MEM (no ...
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bioRxiv - Cell Biology 2020Quote: ... lentiviral particles were concentrated 10-fold using Lenti-X Concentrator (Takara Biosystems 631231).
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected after 48 h and the Lenti-X concentrator (Clontech) was added to the suspension ...
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bioRxiv - Neuroscience 2022Quote: ... Viral-containing supernatants were collected and concentrated with Lenti-X Concentrator reagent (Clontech) according to the manufacturer’s protocol (100X concentration ...
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bioRxiv - Neuroscience 2022Quote: ... Collected lentiviral particles were purified with lenti-X concentrator (Clontech, Mountain View, CA).
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bioRxiv - Cancer Biology 2020Quote: ... which were concentrated using Lentiviral-X Concentration (Takara Bio USA, Mountain View, CA) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral titers were estimated using Lenti-X GoStix Plus (Takara Bio, Cat. # 631281). Lentiviral transduction was performed overnight in the presence of 8μg/ml polybrene (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Lentivirus particles or VLPs were isolated using a Lenti-X Concentrator (321231, Clontech).
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bioRxiv - Molecular Biology 2019Quote: ... after which titer was measured by using Lenti-X-GoStix (Takara Bio, CA). To edit the exon 2 of the human granulin gene in H69 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... The lentiviral medium was subsequently concentrated using Lenti-X™ Concentrator (Takara Bio) and used to infect H1299 cells ...
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bioRxiv - Pathology 2021Quote: ... and the remainder was concentrated with the Lenti-X Concentrator kit (Clontech, 631231). Viral titering was performed using 15 μL of unconcentrated virus or 1.5 μl of concentrated virus with the Lenti-X qRT-PCR Titration Kit (Clontech ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus titer was determined using Lenti-X p24 rapid titer kit (Takara Bio). 2-8 μg/mL puromycin was added gradually to select stable cell lines for two weeks ...
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bioRxiv - Cell Biology 2021Quote: The Lenti-X 293T Cell Line (Takara Bio USA, Inc., Mountain View, CA) was cultured according to the manufacturer’s instructions and transfected with 5 μg pLenti-C-mGFP-P2A-Puro (control ...
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bioRxiv - Bioengineering 2020Quote: ... and lentiviral vectors were concentrated 50-fold using Lenti-X concentrator solution (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral particles were harvested and concentrated using a Lenti-X Concentrator (Clontech; 631231). Concentrated lentiviral particles were supplemented with polybrene (SIGMA ...
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bioRxiv - Microbiology 2021Quote: ... This transfection mix was added to 1.5×106 Lenti-X 293T cells (Takara Bio #632180 ...
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bioRxiv - Cell Biology 2022Quote: ... The virus-containing media were harvested and concentrated with Lenti-X concentrator (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... p24Gag concentrations were quantitated using the Lenti-X p24Gag Rapid Titer Kit (Clontech). For infection ...
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bioRxiv - Biophysics 2022Quote: ... The lentivirus is generated by transfection of Lenti-X 293T cells (Takara, #632180) with pBOB-EF1-FastFUCCI-Puro (Addgene ...
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bioRxiv - Cell Biology 2021Quote: Lentiviruses carrying genes of interest were generated in HEK293T(Lenti-X) cells (Takara) using Lentiviral High Titer Packaging Mix (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... lentivirus was concentrated 10x using lenti-X concentrator (Takara Bio USA, Cat# 631231) with the concentrated pellet being resuspended in PBS with 25 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... lentiviral particles were prepared using the Lenti-X™ Packaging system (Takara Bio). For the pLV-CMV-IRES-PuroR-T2A-mAmetrine vectors ...
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bioRxiv - Neuroscience 2019Quote: ... Concentrated lentiviruses for these two plasmids were made using Lenti-X concentrator (Takara) as has been done previously (Sun et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Physical viral titer was determined using Lenti-X qRT-PCR Titration Kit (Takara), and only viruses greater than 1×1011 GC/ml were used ...
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bioRxiv - Molecular Biology 2019Quote: Sox2 overexpressing SCs were produced using the Retro X ProteoTuner Shield system (Clontech). The Retro X ProteoTuner retroviral vector encodes a 12kDa FKBP destabilization domain (DD) ...
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bioRxiv - Biochemistry 2019Quote: ... Lentivius titers were determined with Lenti-X™ p24 Rapid Titer Kit (Clontech). To infect HUVEC ...
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bioRxiv - Cell Biology 2021Quote: ... Lenti-X 293T cells were purchased from Clontech (no. 632180; Mountain View, CA). These cells were maintained in DMEM (no ...
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bioRxiv - Cell Biology 2021Quote: ... The HEK293-based Lenti-X 293T (HEK293T) cell line was obtained from Takara Bio (Shiga ...
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bioRxiv - Molecular Biology 2020Quote: ... Adenoviruses were produced and amplified in Adeno-X(tm) 293 cells (Takara, # 632271), while titter was determined by fluorescence-activated cell sorting.
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bioRxiv - Immunology 2020Quote: ... Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Cell Biology 2019Quote: ... after which media was collected and virus was concentrated with Lenti-X (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... we employed the Retro-X Tet-On advanced inducible expression system (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Hek293T cells for lentiviral packaging were purchased (Lenti-X 293T cell line, Takara) and expanded to 70-85% confluency in DMEM Glutamax (+ 4.5g/L D-Glucose ...
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bioRxiv - Immunology 2020Quote: Lenti-X™ 293T cells (Takara Bio, Clontech, Saint Germain en Laye, France) were grown using complete Dulbecco’s Modified Eagle Medium F-12 (DMEM F-12 ...
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bioRxiv - Immunology 2020Quote: ... Supernatant was then incubated overnight with Lenti-X concentrator (Clontech, Mountain View, CA) at a ratio of 1:3 ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviruses were generated using the Lenti-X Packaging Single Shot system (Takara Bio), according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... The sequence-verified plasmid was transfected into Lenti-X 293T cells (Takara Bio) together with the packaging plasmid psPAX2 (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... Collected lentivirus was tittered using Lenti-X™ GoStix™ Plus (Takara;631280). Lentiviral transduction was initiated at the first day of Stage 6 with an MOI of 5 for 24 hr.
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bioRxiv - Cancer Biology 2022Quote: ... Produced CAR lentiviruses were concentrated with the Lenti-X Concentrator (Takara Bio, 631232) to increase virus concentration (20X-100X) ...
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bioRxiv - Cell Biology 2022Quote: ... Viral particles were precipitated using Lenti-X maxi-purification kit (Takara, Cat# 631233) based on manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral particles were concentrated from clarified supernatants using Lenti-X Concentrator (Takara Bio). RAW 264.7 cells were transduced with the lentiviral particles and then selected with 4 μg/mL puromycin (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... The retroviral vector in supernatants was concentrated using Retro-X™ Concentrator (Takara) following the manufacturer’s instructions.