Labshake search
Citations for Takara Bio :
801 - 850 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara-Bio) and the following primers ...
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Immunology 2023Quote: ... covering SIV env and nef were amplified from plasma viral RNA by nested RT-PCR using Prime-Script one-step RT-PCR kit v2 (TaKaRa) and KOD-Plus v2 (Toyobo) ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized by PCR and using the divergent primers reported in Supplementary Table 3 and CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Cancer Biology 2023Quote: ... The construct containing Y181G-coding point mutation was generated by inverse PCR of Zdhhc20WT plasmid using CloneAmp HiFi PCR Premix (Takara) and joining the ends of the PCR product using InFusion cloning.
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bioRxiv - Developmental Biology 2023Quote: ... and RT-PCR was performed to detect sorcs2 mRNA in wt embryos using the Titanium One-Step RT-PCR kit (Clontech) with primers 5’-TTTTGCGCACCTGTACCCAGCTG-3’ and 5’-TAACGCGCTCCTGAAGCAGAGTC-3’ for detection of mRNA after injection of different amounts of sMO and 5’-TTTTGCGCACCTGTACCCAGCTGTGTT-3’ and 5’ TAACGCGCTCCTGAAGCAGAGTCCATT-3’ for the different developmental stages ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products of the exons were assembled into one full-length sequence using overlapping PCR (In-fusion cloning, Clontech) for each TAS1R and were then subcloned into the pEAK10 expression vector (Edge Biosystems ...
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Mutations in HUA2 restore flowering in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutantbioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using TB Green™ Premix Ex Taq™ II (Takara, Dalian, China). Relative gene expression was calculated using the 2−ΔΔCt method (Rao et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Final cDNA samples were used then used for quantitative real-time PCR assay by SYBR Green PCR Kit (RR820A, Takara). Quantitative primers were designed based on the gene sequences by Sangon Biotech ...
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bioRxiv - Immunology 2024Quote: ... we ran our linearized plasmid backbone and 83 bp PCR products on a 1% agarose gel and recovered each using the Gel and PCR Clean-up Kit (Takara) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Equimolar amounts of PCR products were mixed and a PCR was made with a primeSTAR GXL DNA polymerase (Takara Bio) without primers for the first 10 cycles using the following thermocycler conditions (95°C 3min ...
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bioRxiv - Biophysics 2024Quote: ... Kir2.1 pathological mutants (C154Y, R312H) were generated by polymerase chain reaction (PCR) with synthetic primers using the CloneAmp HiFi PCR Premix (Takara). Primer sets used for site-directed mutagenesis were the forward sequence ‘5-GTGACTGATGAGTACCCAATTGCAGTGTTTATGGTG –3’ ...
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bioRxiv - Neuroscience 2019Quote: ... PCR products were cloned into pEGFP-C1 (Clontech) using BamHI and SalI ...
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bioRxiv - Plant Biology 2020Quote: ... the In-Fusion Advantage PCR Cloning Kit (Clontech) and Gateway Cloning Technology (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR fragments were cloned into pLVX-puro (Clontech) lentivirus vector with Gibson Assembly Mixture (NEB ...
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bioRxiv - Microbiology 2020Quote: ... One-Step PrimeScript RT-PCR Kit (Takara, RR064) was utilized for qRT-PCR (probe ...
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bioRxiv - Neuroscience 2020Quote: ... and PCR was performed using PrimeSTAR GXL (Takara) with pCAGItdTomato-Rpsa ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by PCR using PrimeSTAR (TaKaRa, R040A). The primers used for monitoring the efficiency of vFLIP knockout are Pr1 (ACCCTGCGTAAACAACCG ...
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bioRxiv - Plant Biology 2021Quote: ... by PCR using PrimeSTAR GXL DNA Polymerase (TaKaRa) and the primer set RenLuc-pPLV26-F/R (Supplementary Table S11) ...
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bioRxiv - Bioengineering 2022Quote: ... or by CloneAmp HiFi PCR Premix (Takara Bio). Random mutagenesis was performed using Error-Prone PCR (EP-PCR ...
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bioRxiv - Genetics 2019Quote: ... PCR was performed using Taq polymerase (TAKR001C, ClonTech) when running DNA fragments on a gel ...
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bioRxiv - Molecular Biology 2020Quote: ... and Nucleospin Gel and PCR Clean-up (Takara). The sequencing library was sequenced with the Illumina Next-Seq 500 instrument (Illumina ...
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bioRxiv - Genetics 2021Quote: ... PCR was performed using PrimeSTAR HS (Takara Bio) and T100 Thermal Cycler (Bio-rad ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were digested with DpnI (TaKaRa) at 37°C for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... and In-Fusion Advantage PCR Cloning Kit (Clontech) were used ...
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bioRxiv - Genetics 2020Quote: ... PCR products amplified using LA Taq polymerase (Takara) or Multiplex PCR Kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Mutagenic PCR reactions used PrimeStar GXL polymerase (Takara), and 45mer mutagenesis primers (Table S2 ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP was PCR-amplified from pEGFP-C2 (Clontech) and similarly cloned into the pRSET His-SS-TEV vector ...
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bioRxiv - Immunology 2020Quote: ... The riboswitch was amplified by PCR (CloneAmp, Takara; according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Using PCR and In-Fusion cloning (Takara Bio), the ‘NGG’ PAM-recognition domain of the prime editor protein (PE2 ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed with Ex-Taq enzyme (TaKaRa) in a total volume of 30 µl containing 1 µl viral DNA ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed using Taq polymerase (TAKR001C, ClonTech) when running DNA fragments on a gel ...
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bioRxiv - Biochemistry 2022Quote: ... PCR was performed using KOD DNA polymerase (Takara) with the following settings ...
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bioRxiv - Neuroscience 2022Quote: ... PCR amplification (PrimeSTAR Max polymerase, Takara Bio USA), and In-Fusion cloning (In-Fusion Snap Assembly Master Mix ...
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bioRxiv - Plant Biology 2022Quote: The In-Fusion Advantage PCR Cloning Kit (Clontech) and Gateway Cloning Technology (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 1x of PCR buffer (10x, Takara, CA, USA), 10 μL of barcoded primer set forward/ reverse from ONT ...
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bioRxiv - Microbiology 2023Quote: ... In the In-Fusion PCR cloning system (Clontech), the S1 or S2 gene of the HV80 strain was replaced with the corresponding region of the H120 strain to construct the recombinant plasmids pMDVM8-HV80S1H120S2 and pMDVM8-H120S1HV80S2 ...
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bioRxiv - Developmental Biology 2023Quote: ... using CloneAmp™ HiFi PCR Premix (Takara 639298) and cloned it (in frame ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan)
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bioRxiv - Cell Biology 2023Quote: ... using a Takara Thermal PCR Cycler (Takara Bio). RT-PCR of miRNAs was performed with a Taqman microRNA Reverse Transcription Kit (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: For PCR reactions PrimeSTAR HS DNA Polymerase (Clontech), Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed using Taq polymerase (TAKR001C, ClonTech) when running DNA fragments on a gel ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was conducted using PrimeSTAR Max (Takara) or KOD-Plus-Neo (TOYOBO).
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bioRxiv - Genetics 2023Quote: ... Then One-step PrimeScript RT-PCR kit (Takara) was used to generate cDNA ...
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bioRxiv - Plant Biology 2023Quote: ... the PCR products were digested with SifI (Takara) and cloned into the empty pCambia1305-3xFLAG or pCambia1305-3xHA vectors (gifted by Dr Yuli Ding ...
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bioRxiv - Genetics 2023Quote: ... and PCR amplified into 600 bp products (Takara). Indel frequencies were determined through Sanger data-decomposition using TIDE analysis tool ...
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bioRxiv - Immunology 2023Quote: ... Kit Advantage 2 PCR Enzyme System (Clontech, 639206), QIAquick Gel Extraction ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed using Titanium Taq (Takara, #639208) with an extension time of 90s for 30 (1st round ...
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bioRxiv - Pathology 2024Quote: ... SYBR Green PCR reagent (Takara Co. Ltd., Japan) was chosen for real-time fluorescence quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCR analyses were performed using PrimeStarGXL (TaKaRa) as follows ...