Labshake search
Citations for Takara Bio :
601 - 650 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... pneumophila (RefSeq NC_002942.5) genomic DNA by PCR using CloneAmp HiFi PCR premix (Takara) and the relevant primers ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed in triplicate using an SYBR Green PCR kit (Takara, #RR820A) in a 10 µl volume ...
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bioRxiv - Plant Biology 2020Quote: ... 3′ RACE-PCR was performed using the SMARTer PCR cDNA synthesis kit (Clontech) following the instructions supplied by the manufacturer ...
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bioRxiv - Systems Biology 2021Quote: ... all PCRs utilized a high fidelity polymerase (CloneAmp HiFi PCR Premix, Takara 639298). Plasmids contained components of the synthetic inducible system flanked by homology to the intended genomic integration locus and were restricted prior to transformation ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR was carried out using the One Step RNA PCR Kit (TaKaRa Biotechnology Dalian ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using Nucleospin Gel and PCR Clean-up kit (Takara), and Sanger sequenced using primer IGHG1-F1 (Supplementary Table 5 ...
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bioRxiv - Immunology 2023Quote: ... the remaining PCR products were extracted with PCR cleanup kit (TaKaRa Bio USA) and sequenced to confirm the correct gene sequence.
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bioRxiv - Neuroscience 2023Quote: PCR amplifications for cloning were performed with the CloneAmp HiFi PCR Premix (Clontech) and plasmid fusion/reassembly were performed with the NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Genetics 2024Quote: Long-range PCR was performed using the TaKaRa LA PCR kit (TaKaRa Bio). PCR conditions were optimized for the extension-annealing phase ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Real-time PCR was performed with SYBR RT-PCR kit (Takara, Dalian, China). The mRNA levels were normalized against β-Actin and presented as 2-ΔΔCt.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Real-time PCR was performed with SYBR RT-PCR kit (Takara, Dalian, China). The mRNA levels were normalized against β-Actin and presented as 2-ΔΔCt ...
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bioRxiv - Genetics 2020Quote: ... First-round reverse transcription PCR (RT-PCR) was conducted by using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). A 10 μL reaction mixture contained 5 μL of 2 × 1 Step Buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... reactions were performed using the Advantage 2 Polymerase Mix (Clontech) using the primers (Merck ...
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bioRxiv - Systems Biology 2019Quote: ... and 3× PrimeScript enzyme mix (Cat# RR037A, TAKARA Bio INC) in RNase-free water ...
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bioRxiv - Microbiology 2023Quote: ... using a DNA Ligation Kit (Mighty Mix, TaKaRa, Cat# 6023). The ligated constructs were then transformed in NEB 5-alpha F′ Iq Competent E ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... Cloning was performed using In-Fusion HD Cloning Mix (Takara). Restriction enzymes were purchased from New England Biolabs (NEB) ...
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bioRxiv - Cell Biology 2020Quote: Reverse transcription-PCR (RT-PCR) was performed using the PrimeScript RT Reagent Kit (TaKaRa). Quantification of mRNA level was performed in a 20 μl mixture consisting of 10 μl Q5 High-Fidelity 2X Master Mix (NEB) ...
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed in the TaKaRa PCR Thermal Cycler Dice Touch (TaKaRa, Shiga, Japan) using the Tks Gflex DNA Polymerase (TaKaRa) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was conducted using a Takara PCR thermal cycler Dice mini (Takara Bio Inc.) as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were purified with NucleoSpin Gel and PCR Clean-Up (Takara Bio, #740609). 400 ng of PCR products mixed with NEB buffer 2 were denatured at 95°C for 5 minutes and ramped down to 25°C ...
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bioRxiv - Genomics 2022Quote: ... PCR products were purified using NucleoSpin® Gel and PCR Clean-Up Kit (Clontech). IVT was performed to generate dsRNA using the T7 High Yield RNA Synthesis Kit (NEB) ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... The PCR reaction was performed using Terra PCR Direct Red Dye Premix (#639286, TaKaRa) for LSL-KRas construct detection and GoTaq Green Master Mix (#M7122 ...
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bioRxiv - Microbiology 2020Quote: ... Each PCR reaction contained 2.5 µl of PCR buffer (TaKaRa, 10x concentration, 1x final), 1 µl of the Golay barcode tagged forward primer (10 µM concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (TaKara Bio Inc.), and then analyzed and quantified by Nanodrop spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... followed by RT-PCR using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa) with primers no ...
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bioRxiv - Zoology 2023Quote: ... The PCR products were purified using Nucleospin Gel and PCR Clean-up (Takara Bio). Nucleotide sequence analysis was outsourced from a commercial sequencing service (Eurofins ...
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bioRxiv - Biochemistry 2023Quote: ... qRT-PCR was carried out using SYBR Premi qRT-PCR ExTaq™ II (Takara) and analyzed on a Bio-Rad CFX96 real time PCR cycler (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: The quantitative RT-PCR (qRT-PCR) was performed with SYBRGreen II (Takara, Dalian, China). The 7500 system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by PCR with the SeqAmp PCR kit (Takara Bio USA, San Diego, CA). Unlike TARGET-Seq ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (TaKara Bio Inc.), and then analyzed and quantified by Nanodrop spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: CloneAmp HiFi PCR Premix (Takara) was used for all PCR amplifications ...
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bioRxiv - Molecular Biology 2022Quote: ... by RT-PCR (TaKaRa, RR014) and cloned by Gibson assembly into the vector pK27SUMO (expressing a 14His-tagged version of the yeast SUMO protein Smt3 ...
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bioRxiv - Microbiology 2021Quote: ... 1× ExTaq PCR buffer (Takara, Clontech Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR Green PCR kit (Takara) was applied to conduct RT-qPCR ...
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bioRxiv - Microbiology 2021Quote: ... using CloneAmp HiFi PCR (Takara) and was subcloned in the Thymidine kinase Cypridina plasmid (Thermofisher ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 μL PCR mixture (TaKaRa), 1 μL upstream primer (10 μmol/L) ...
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bioRxiv - Microbiology 2021Quote: ... Comparative RT-PCR (Takara, Japan) was performed using SYBR Green Supermix via an RT-PCR machine (RocheLightCycler480 ...
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bioRxiv - Biochemistry 2023Quote: ... by RT-PCR (Takara, RR014) and cloned by Gibson assembly into the vector pK27SUMO ...
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bioRxiv - Biophysics 2023Quote: ... CloneAmp HiFi PCR Premix (Clontech) was used for all the PCR amplifications ...
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bioRxiv - Plant Biology 2023Quote: ... PCR was performed by TaKaRa EX Taq (Takara ...
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bioRxiv - Immunology 2023Quote: ... and the cDNA was synthesized using the PrimeScript RT master kit (Takara, RR036A). The qPCR was done using PowerUp SYBR Green Master Mix (Thermofisher ...
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bioRxiv - Evolutionary Biology 2020Quote: PCR amplification was carried out using the Advantage GC 2 PCR Kit (Clontech Catalog #639119), and cloning was carried out using standard procedures (primer sequences are listed below).
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bioRxiv - Plant Biology 2019Quote: ... Real-time PCR (qRT-PCR) was determined by using SYBR Premix Ex Taq II (TaKaRa) and performed on ABI Stepone real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was done using SYBR/ TB Green RT PCR kit (Takara Bio) in Bio- Rad real-time PCR detection system ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was done using SYBR/ TB Green RT PCR kit (Takara Bio) in Bio-Rad real-time PCR detection system ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCRs were performed using a SYBR PremixEX Taq II (RT)-PCR kit (Takara, Japan) on a Thermo PIKOREAL 96 real-time PCR System ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were performed with CloneAmp HiFi PCR premix (Takara Bio, Mountain View, California) using 25 ng of DNA ...
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bioRxiv - Zoology 2020Quote: ... Reverse transcription-PCR was conducted using a PrimeScript One-Step RT-PCR kit (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and PCR reaction was performed using a miR-X miRNA qRT-PCR SYBR Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The final PCR product was subcloned into pCS2+ by Infusion PCR (Takara Bio, Kusatsu, Japan). For subcloning into N’3xGFP-pCS2+ ...