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Citations for Takara Bio :
951 - 1000 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... template DNAs were amplified by PCR (PrimeSTAR Max, Takara) with the 4 overlapping DNA oligos (three scaffolding oligos and one target-specific oligo ...
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bioRxiv - Developmental Biology 2020Quote: ... GFP was amplified via PCR from pEGFP-N1 (Clontech) and inserted into the linearized pBS-KSdsh vector using Gibson Assembly to generate pBS-KSdsh-GFP ...
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bioRxiv - Neuroscience 2020Quote: ... a PCR performed using PrimeStar GLX Polymerase (Takara Clontech) with pAAV.Syn.GCaMP6s.WPRE.SV40 (Addgene Plasmid#100843 ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions were performed with SeqAmp DNA Polymerase (Takara), a proofreading polymerase ...
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bioRxiv - Microbiology 2021Quote: ... PCR was conducted with Ex Taq (TaKaRa Bio, Inc.) in accordance with the CRISPR protocol (Supplemental Methods).
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bioRxiv - Immunology 2021Quote: ... or KAPA Taq PCR Kit (Takara Bio, cat. R040A). Primers Rosa26 WT F ...
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bioRxiv - Developmental Biology 2020Quote: ... T2ATdTomato sequence was generated by PCR of pTdTomato (Clontech). The integrity of the vector was tested by sub-cloning the entire expression cassette into CAG IRES Puro vector and the Tdtomato signal confirmed in transient transfected ESCs by flow cytometry.
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bioRxiv - Genomics 2019Quote: ... 0.48-μM IS PCR Primer (12 μM, Clontech, 639206), 2X Advantage 2 Polymerase Mix (50X ...
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bioRxiv - Molecular Biology 2022Quote: We use a PCR amplification kit (Takara Bio, Japan) to obtain the purified dsDNA target and control with primers listed in Table 1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCRs used the PrimeSTAR GXL DNA Polymerase (Takara Bio) under conditions following the protocol listed by the supplier ...
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bioRxiv - Microbiology 2022Quote: ... SMARTer® PCR cDNA synthesis kit (TaKaRa,cat 634926) and PrimeSTAR® GXL DNA polymerase (TaKaRa ...
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bioRxiv - Microbiology 2022Quote: ... and the SmartChip Real-Time PCR system (Takara Bio). The SmartChip MyDesign chip contains 5,184 nanowells ...
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bioRxiv - Immunology 2022Quote: ... before amplification using the Advantage 2 PCR kit (Clontech) and the SINGV6 primer (95°C for 1 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Each domain deletion PCR amplification was performed by TAKARA-HIFI Tag by following the manufactory guides ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was conducted using TB Green fluorescence (TaKaRa) on a LightCycler 480 II System (Roche Diagnostics ...
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bioRxiv - Microbiology 2023Quote: ... PCR (PrimeSTAR MAX DNA polymerase, Takara Bio USA, Inc.) was performed using divergent primers flanking or spanning the junction site of the specific circRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA levels were measured using qRT-PCR kit (Takara) in combination with TB Green real-time PCR ...
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bioRxiv - Microbiology 2023Quote: Polymerase chain reaction (PCR) was conducted using ExTaq (TaKaRa) to amplify the SLST locus ...
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bioRxiv - Molecular Biology 2023Quote: ... All the DNA templates were prepared by PCR (Takara) unless otherwise noted ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by PCR amplification using Ex-Taq polymerase (Takara) and template cDNA ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was conducted using TB Green fluorescence (TaKaRa) on a MasterCycler EP Realplex 4 thermal cycler (Eppendorf ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using KOD DNA polymerase (Takara Bio) with the following settings ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was ligated with InFusion ligation enzyme (TaKaRa) on EPB71 backbone (Addgene plasmid #90018 ...
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bioRxiv - Microbiology 2023Quote: ... Viral cDNA was PCR amplified using CloneAmp HiFi (TaKaRa) in four overlapping amplicons of about 2.2 kb ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was treated with DpnI (TaKaRa Bio) at 37 °C for 1 h and transformed into Escherichia coli JM109 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using the Advantage® 2 PCR Kit (Takara Bio) in a touchdown cycling program as follows ...
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bioRxiv - Microbiology 2020Quote: ... The amplification of viral DNA by PCR was carried out base on the manufacturer’s recommendations of the LA PCR Kit (TaKaRa Biomedical Technology Beijing, China). Briefly ...
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bioRxiv - Pathology 2022Quote: ... PCR was performed using Quick Taq HS DyeMix (Toyobo, Osaka, Japan) and a PCR Thermal Cycler Dice (TP650; Takara Bio Inc., Shiga, Japan), with the following cycling conditions ...
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bioRxiv - Microbiology 2020Quote: ... and qRT-PCR was performed on StepOne Plus Real-time PCR system (Applied Biosystem) with TB Green Premix Ex Taq II (Takara Cat no. RR820A). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription of the total RNA to complementary DNA and amplification of cDNA templates by long-distance PCR (LD-PCR) were performed using SMARTer Ultra-low Input RNA for Illumina Sequencing-HV (Clontech Labaratories, CA, USA) kit ...
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bioRxiv - Microbiology 2021Quote: RsmY and RsmZ expression quantification was done by RT-PCR using Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio USA, Inc). For each reaction ...
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bioRxiv - Plant Biology 2019Quote: High-throughput qRT-PCR reactions were performed using the WaferGen SmartChip Real-time PCR system (5184-nanowells chip, WaferGen Bio-systems and Takara Bio Inc. USA). qRT-PCR reaction volumes of 100 nL consisted of 1 X LightCycler 480 SYBR Green I Master (Roche Diagnostics France) ...
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bioRxiv - Plant Biology 2020Quote: ... The band intensity of products obtained from semi-Q PCR was observed on the agarose gel and was further characterized with qRT-PCR using SYBR Green ® Premix (Takara Bio, USA). Specific primers were designed for studying expression of stress related genes in Arabidopsis and RP genes in rice using primer3 (v.0.4.0 ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... was performed by quantitative real-time polymerase chain reaction (PCR) using the AAVpro™ Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Polymerase chain reaction (PCR) amplification of DNA receivers for plasmids construction and colony PCR were performed using PrimeSTAR® Max DNA Polymerase (R045B, TaKaRa, Kyoto, Japan). PCR primers were synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Genomics 2023Quote: Specimens were run in technical duplicates for tprK deep sequencing using 10.5µL DNA added to 12.5µL 2X CloneAmp Pre-mix (Takara), 1µL 10µM barcoded tprK-F and 1µL 10µM barcoded tprK-R (Table S2 in Supplemental Data) ...
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bioRxiv - Neuroscience 2023Quote: ... a mix of an mCherry-DsRed rabbit polyclonal antibody (Living Colors-Clontech Antibody 632496, 1:1000) and a mouse monoclonal antibody against oxytocin (P38 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% Tween 20) and resuspend with Capture mix (50µl per ROI_index: 2xBWT, 0.5U/µl RRI (Takara)) ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated using a template□switch anchored reverse□transcription polymerase chain reaction (RT□PCR) based on the SMARTer pico cDNA PCR Synthesis Kit (Clontech, Mountain View, CA, USA). The final cDNA product was then subjected to a clean□up step using PCR Clean DX Beads (Aline Biosciences ...
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bioRxiv - Plant Biology 2020Quote: ... were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara) and gene-specific primer sets ...
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bioRxiv - Cell Biology 2020Quote: ... sgRNA inserts were PCR amplified using ExTaq DNA Polymerase (Takara). The resultant PCR products were purified and sequenced on a HiSeq 2500 (Illumina ...
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bioRxiv - Genetics 2021Quote: ... Colony PCR was performed using Takara Taq polymerase (Clontech, TAKR001C). Plasmid DNA was isolated from cultured bacteria using QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... or 1× Nested Universal Primer (secondary PCR, Clontech Laboratories, Inc.) and gene-specific primers ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed via SYBR Premix Ex Taq (Takara) and Rotor-Gene 3000 system (Corbett Research) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed with PrimeSTAR GXL DNA polymerase (Takara) using gene-specific primers (Table S7) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR reaction consisted of PrimeSTAR Max Premix 1x (Takara Bio), 0.4 μM of each primer and 30-100 ng DNA template ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was conducted using PrimeSTAR Max DNA Polymerase from TaKaRa. The PCR products were checked by agarose gel electrophoresis and inserted into pET28a plasmid between the BamHI and EcoRI sites with Gibson Assembly method ...
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bioRxiv - Immunology 2019Quote: ... The RACE PCR products were cloned into pMD-19Tvector (Takara) and sequenced.