Labshake search
Citations for Takara Bio :
751 - 800 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... or CloneAmpTM HiFi PCR Premix (Takara Bio) using the corresponding primers listed in TableS1 ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using TaKaRa ExTaq (TaKaRa). PCR products were analyzed using a 1% agarose gel ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed with ExTaq (TaKaRa, Japan) on 2720 thermal cycler (Applied Biosystems) ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: CloneAmp HiFi PCR Premix was from Takara. Primers were synthesized by Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Bisulfite PCR was performed using EpiTaq (Takara) using custom made locus specific primers containing 4 bp unique molecular identifiers (UMI ...
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bioRxiv - Developmental Biology 2023Quote: ... Nested PCR was performed with rTaq (Takara), followed by the PCR product fragments sequencing with Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted gDNA was PCR amplified (Takara, RR001A) using a 30-cycle protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was performed using PrimeSTAR MAX (TAKARA) with primers (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments containing pLib vector backbone (Clontech) and EF1A promoter ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Its male-specific exon was amplified using Advantage® 2 Polymerase Mix (TaKaRa), the gene-specific primer “Cpun_dsx OD2 3’RACE” ...
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bioRxiv - Microbiology 2021Quote: ... This transfection mix was added to 1.5×106 Lenti-X 293T cells (Takara Bio #632180 ...
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bioRxiv - Microbiology 2022Quote: ... was performed with one-step Prime script III RT-qPCR mix (Takara, Japan). The viral RNA of NP was detected by 2019-nCoV-N1 probe (Cat#10006770 ...
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bioRxiv - Microbiology 2024Quote: ... comprising 25 µl of 2x probe qPCR mix (Takara Bio Inc., Otsu, Japan), 0.2 µl each of forward and backward primers (100 µmol l-1 each ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... All mutagenesis was done using instructions from In-Fusion HD Cloning Mix (Takara) unless noted below.
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bioRxiv - Molecular Biology 2024Quote: qPCR was performed using TB Green Fast qPCR Mix (catalog no. RR430A, TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 20 μL reaction containing SYBR Green (TaKaRa, Kusatsu, Japan) on MXP3000 cycler (Stratagene ...
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bioRxiv - Genomics 2021Quote: ... tricornutum were amplified by PCR was amplified by PCR using PrimeSTAR Max DNA polymerase (TaKaRa Bio Inc., Shiga, Japan) and the primer (pFs_nptII_1_F ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR reactions were performed with gene-specific forward and reverse primers using the PrimeScript RT-PCR kit (TaKaRa) on the StepOne Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR) (Takara Cat no.RR820A) to reflect the replication efficiency of the virus ...
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bioRxiv - Systems Biology 2022Quote: ... The DNA regions of interest were amplified from host plasmids by PCR using CloneAmp HiFi PCR Premix (Takara, 639298) with custom primers for Gibson assembly (with overhangs) ...
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bioRxiv - Biochemistry 2022Quote: ... N-terminal Flag-tagged P38α containing 3C protease site was amplified by PCR using CloneAmp HiFi PCR Premix (Takara) and ligated in to pcDNA3 using the aforementioned restrictions sites ...
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bioRxiv - Cell Biology 2020Quote: ... was used to PCR amplify the PLIN2 cDNA and gel purified via NucleoSpin Gel and PCR Clean Up (Takara, Mountain View ...
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bioRxiv - Molecular Biology 2022Quote: ... one subjected to RT-PCR using PrimeScript™ One Step RT-PCR Kit Ver.2 (Dye Plus) (Takara, Japan), then the region between S-5’UTR and N protein-ORF genes was amplified to monitor the expression of eGFP.
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bioRxiv - Evolutionary Biology 2022Quote: ... The product from this PCR was purified and cleaned with NucleoSpin Gel and PCR clean-up kit from TAKARA. A 2nd PCR was performed with high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and then used as the template to synthesize double strand cDNA amplicons by PCR (optimal 17 – 21 cycles) using Advantage 2 PCR kit according to the manufacturer’s specifications (Clontech). cDNA was purified using NucleoSpin Gel and PCR Clean-up kit (Takara Bio) ...
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bioRxiv - Microbiology 2024Quote: ... The guides were introduced into the pEcgRNA vector through reverse PCR using the CloneAmp HiFi PCR Premix (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).
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bioRxiv - Cancer Biology 2024Quote: ... Reverse-transcription-quantitative PCR (RT-qPCR) was conducted using the Mir-X miRNA qRT-PCR TB Green Kit (TAKARA), the reverse-transcription samples ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... The synthesized cDNA was diluted (1:10) and used as template for quantitative real-time PCR (qRT-PCR) using SYBR premix EX-Taq-plus (TaKara) and analyzed on QuantStudio 6 Real-Time PCR machine (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa) with each specific primer (Table S2).
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bioRxiv - Cell Biology 2019Quote: ... DNA was isolated from the expanded single colonies when confluent enough and used in PCRs using oligos to amplify exon 2 of CLN6 with CloneAmp HiFi PCR Premix (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: The target fragments are amplified by PCR using primers (Sangon Biotech, Shanghai, China) and PCR amplification kit (Takara Bio, Japan). The PCR amplifies the DNA fragments including the 1900 bp target ...
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bioRxiv - Immunology 2020Quote: ... The coding region of ADAR1 p150 was amplified by PCR and the resultant PCR product was inserted into a pEGFP-C1 vector (Clontech) using XhoI/BamHI restriction enzyme–recognition sites to generate the expression construct ...
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bioRxiv - Physiology 2021Quote: ... The RNA (1 ug/sample) was reverse transcribed into cDNA and Q-PCR was performed using a SYBR Green PCR kit (Takara) in a Light Cycler (Eppendorf) ...
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bioRxiv - Microbiology 2020Quote: ... The 1.5 kb PCR product was isolated and purified from agarose gel using the NucleoSpin Gel and PCR Clean-up kit (Takara). The product was subjected to Sanger dideoxy sequencing at GENEWIZ (San Diego ...
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bioRxiv - Cell Biology 2021Quote: ... RT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time; Takara, Japan) and Thermal Cycler Dice® Real Time System Lite (TP700 ...
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bioRxiv - Cell Biology 2021Quote: Site-directed mutagenesis of pLenti-CMV-Neo-PINK1 (C125G)-EYFP or pLVX-puro-OMA1 (E328Q)-EYFP was performed by PCR amplification (CloneAmp HiFi PCR Premix, Takara or Q5 High-Fidelity DNA Polymerase system ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated using the TRIzol method and the expression of miRNAs analyzed by qRT-PCR with the Mir-X miRNA qRT-PCR TB Green kit (Takara Bio Inc ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time reverse transcription-PCR (qRT-PCR) was performed using the TB Green Premix Ex Taq II (TaKaRa, Japan) with the LightCycler® 96 system (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The elute was diluted such that no more than 1.5ug of DNA was used per PCR reaction and amplified for 20 cycles of PCR using Primestar GXL (Takara Bio), run on a 1% agarose gel ...
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bioRxiv - Biophysics 2022Quote: ... eGFP-PolyQ31 was adapted from eGFP-PolyQ74 through the variability of Q-length PCR products amplified using CloneAmp HiFi PCR Premix (Takara). FM5-NPM1-mCh was generated as described in 27.
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bioRxiv - Cell Biology 2022Quote: All plasmids construction was performed by PCR amplification (CloneAmp HiFi PCR Premix) using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system, Clontech) into the EcoRI site of pLVX-puro vector (Clontech ...
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bioRxiv - Zoology 2019Quote: ... were obtained from the mRho.V5.mER.hOr47a construct via PCR using the Advantage 2 PCR kit (Cat. Nr. 639206, Takara, Kusatsu, Japan) using the E.hOr47a_fwd and hOr47a_fwd forward primers ...
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bioRxiv - Molecular Biology 2021Quote: ... A 150-250 bp region encompassing each targeted locus was PCR amplified from ∼40 ng genomic DNA with ends containing partial Illumina adapter sequences using CloneAmp HiFi PCR (Takara). Reaction conditions were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... Homology arms complementary to the pUC19 cloning vector were then added to the PCR product with another PCR in order to clone the RACE products with the In-Fusion HD Cloning kit (Takara). Individual clones were selected and Sanger sequenced.
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bioRxiv - Cell Biology 2022Quote: ... cDNA was amplified by PCR by adding 1 µl of the Terra PCR Direct Polymerase (1.25 U/µl, Takara 639270), 25 µl of the Terra PCR Direct buffer and 1 µl of the ISPCR primer (10 µM stock concentration ...
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bioRxiv - Plant Biology 2022Quote: ... were chemically synthesised and cloned by PCR into the pJET1.2 based gRNA vectors using Clontech CloneAmp HiFi PCR Premix (Takara, Japan). The transformation vector pICU2:Cas9-dsRED containing the Cas9 gene from Streptococcus pyogenes expressed under the promoter of the Arabidopsis Incurvata 2 gene (ICU2 ...
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bioRxiv - Microbiology 2023Quote: ... and were quantified by real-time RT-PCR using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on three biological replicates using the Thermal Cycler Dice Real Time System TP-760 (Takara) with the Kapa SYBR fast universal qPCR kit (Kapa Biosystems) ...