Labshake search
Citations for Takara Bio :
901 - 950 of 2841 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed using TB Green Premix Ex Taq ™ II (Tli RNaseH Plus) (Takara) with qTOWER3 (Jena) ...
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bioRxiv - Microbiology 2024Quote: ... the LHA and RHA PCR products were used as primers for amplifying the tsp exchange gene using CloneAmp HiFi PCR Premix (Takara Bio). After five PCR cycles ...
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bioRxiv - Microbiology 2024Quote: ... the tsp gene and 500 base pair upstream and downstream of the S117 tsp gene that should be exchanged (Left and Right Homology Arms (LHA and RHA)) were amplified by PCR using CloneAmp HiFi PCR Premix (Takara Bio). LHA and RHA primers carried overhangs with homology to the tsp exchanged gene ...
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bioRxiv - Immunology 2021Quote: ... The transcripts of the DEMs and DEPs were analyzed and validated by qRT-PCR assays using the Mir-X miRNA qRT-PCR SYBR® Kit (TaKaRa, Japan) and the PrimeScriptTM RT reagent Kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the primers of the U6 snRNA used in the qRT-PCR assays were provided in the Mir-X miRNA qRT-PCR SYBR® Kit (Takara, Japan). The qRT-PCR data were analyzed by the 2−ΔΔCt method as described in Livak & Schmittgen (2001 ...
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bioRxiv - Pathology 2020Quote: The viral loads of WHCV in BALF of patient 1 were determined by quantitative real-time RT-PCR with Takara One Step PrimeScript™ RT-PCR Kit (Takara RR064A) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were purified using Nucleo Spin® Gel and PCR Clean-up Kits (Code No. 740609.50; TAKARA Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Hmx1 putative enhancer elements were amplified by PCR from total genomic DNA using the LA Taq PCR kit v2.1 (TaKaRa, Mountain View, CA) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... levels were measured by a qRT-PCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A). The PCR protocol was 42°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured again overnight and subjected to the quantification of mRNA by qRT-PCR using the CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (3732, Takara Bio Inc.), One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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bioRxiv - Plant Biology 2023Quote: ... The expression of marker genes was detected by qRT-PCR using the One-step TB Green PrimeScriptTM RT-PCR kit II (Takara, Osaka, Japan). All genes were normalized against the level of an actin reference gene (Foo et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed using the TB Green Premix Ex Taq II (Takara Bio, San Jose, CA, USA) under the following cycling conditions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Gene-specific mRNA levels were assessed by qPCR using TB Green Fast qPCR Mix (Takara) on a LightCycler 480II instrument (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... and a final extension at 72 °C for 10 min using Advantage2 polymerase mix (Clontech); specific primers were designed for cloning the putative spexin ligands and receptors (Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... the annealed oligo duplex at a 1:3 mol ratio and ligation mix (Takara Bio) at 16 °C for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μl of Ligation Mix (5 mM ATP, 7U Terminal Deoxynucleotidyl Transferase (TdT) (2230B, Takara), 15 U T4 RNA Ligase High Concentration (M0437 ...
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bioRxiv - Pathology 2022Quote: ... 200 ng of total RNA was reverse transcribed using cDNA Synthesis Mix (Takara, Dalian, China). Real-time PCR was carried out in an Applied Biosystems QuantStudio 5 Real-Time PCR System (ABI ...
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bioRxiv - Microbiology 2022Quote: ... was performed using one-step Prime script III RT-qPCR mix (RR600A, Takara, Kyoto, Japan). The viral RNA of nucleocapsid protein was detected by a 2019-nCoV-N1 probe (10006770 ...
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bioRxiv - Immunology 2020Quote: ... 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was labelled with SYBR Pre-mix Ex Taq (Tli RNase H Plus, from Takara) and the Cq values were obtained from the CFX96 TouchTM Real-Time PCR Detection System (BioRad).
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bioRxiv - Molecular Biology 2019Quote: ... DNA was labelled with SYBR Pre-mix Ex Taq (Tli RNase H Plus) from Takara and the Cq values were obtained from the CFX96 TouchTM Real-Time PCR Detection System (BioRad).
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bioRxiv - Genomics 2020Quote: ... 3μl of reverse transcription (RT) mix was added (0.475μl SmartScribe [Takara], 0.125μl RNase inhibitor [Takara, cat no ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence (CDS) of SAUR30 was cloned using Prime STAR MAX polymerase mix (TaKaRa) and the gene-specific primers shown in Supplemental Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Reaction mix contained 7.5 µL SYBR TB Green® Premix Ex Taq™ (RR420A, Takara) with 0.5 µM forward and reverse primers and 2.5 µL of each cDNA sample ...
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bioRxiv - Microbiology 2023Quote: ... was performed using one-step Prime script III RT-qPCR mix (Takara Bio, Shiga, Japan). The viral RNA of ZIKV NS3 was detected by customized probe-based qPCR assay (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmid was transfected into 293T cells using Lentiviral High Titer Packaging Mix (Takara) to obtain the lentiviral vector for TSG101–venus transduction ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR was performed using LA Taq (TAKARA Bio). The primer pairs employed for RT-PCR are listed in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2021Quote: PCRs for were undertaken using ExTaq DNA Polymerase (TaKaRa). Primers used are listed below.
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bioRxiv - Neuroscience 2020Quote: ... a PCR performed using PrimeStar GLX Polymerase (Takara Clontech) with pAAV.Syn.GCaMP6s.WPRE.SV40 (Addgene Plasmid#100843 ...
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bioRxiv - Developmental Biology 2019Quote: ... Probe regions were PCR amplified with Ex Taq (Takara) directly from genomic DNA or cloned plasmid templates to incorporate DIG-labeled dUTP (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR was performed with Advantage cDNA Polymerase (Clontech, 639105) and products were cloned into the pGEM-T Easy vector (Promega ...
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bioRxiv - Molecular Biology 2019Quote: End-point PCR was performed with SapphireAmp (Takara, RR350B) by denaturation at 95°C for 5min ...
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bioRxiv - Plant Biology 2021Quote: ... Procedure for PCR amplification by using ExTaq polymerase (Takara): 94°C 2 min ...
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bioRxiv - Biochemistry 2021Quote: ... qRT-PCR was conducted using TB Green fluorescence (TaKaRa) on a LightCycler 480 II System (Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were performed using rTaq (Takara biotechnology, Dalian, China) or KOD (Toyobo ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed using SYBR Premix ExTaq (TaKaRa) on a CFX96 Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... PCR was conducted using ExTaq (Takara Bio, Kusatsu, Japan) and amplicons were visualized on a 1.4% agarose gel.
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using PrimeSTAR Max DNA Polymerase (Takara) with the cDNA as template and virus-specific primers 5’-CAC TCT TTC CCT ACA CGA CGC TCT TCC GAT CT NNN GGT CAC TAG TTG CCT CAT CCG G-3’ and 5’-GAC TGG AGT TCA GAC GTG TGC TCT TCC GAT CTG GTG CAT CTG ATC TCA TTA TTG-3’ ...
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bioRxiv - Bioengineering 2020Quote: ... Lenti-X™ qRT-PCR Titration Kit (Takara biosciences) was used to calculate the viral multiplicity of infection (MOI ...
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bioRxiv - Genetics 2022Quote: ... the mCherry sequence amplified by PCR from pmCherry (Clontech) was inserted into pBS-UASp (pBS-UASp-mCherry) ...
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bioRxiv - Developmental Biology 2022Quote: ... Trim33 PCR was performed using LA Taq polymerase (Takara) and the following primer pair:
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bioRxiv - Microbiology 2022Quote: ... PCR amplification was performed by using PrimeStar MAX (TaKaRa) and 14 specific primer sets tiling the genome of PaBV-4 (shown in Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2019Quote: ... PCRs were run with PrimeSTAR GXL DNA Polymerase (Takara) using the following thermal cycling program ...
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bioRxiv - Cancer Biology 2020Quote: ... SYBR PrimeScript primary RT-PCR kit (Takara Bio, Japan) was used to evaluate the expression levels of METTL3 ...
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bioRxiv - Cell Biology 2020Quote: ... eGFP was PCR-amplified from pEGFPN1 (Takara Bio USA) and inserted using Gibson assembly into a NotI/BamHI-digested pACT (Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... The DNA fragments amplified by high fidelity PCR (Takara) were digested with the appropriate restriction enzymes ...
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bioRxiv - Developmental Biology 2020Quote: ... retro-transcribed using the PrimeScript RT-PCR Kit (Takara) and analysed via qPCR using the SYBR qPCR Premix Ex Taq (Takara ...
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bioRxiv - Plant Biology 2019Quote: ... in a TP800 Real-Time PCR machine (Takara, Japan). The poplar Ubiquitin gene was used as the reference gene as an internal standard ...
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bioRxiv - Immunology 2021Quote: ... Standards were prepared from a PCR PrimeStar MAX (Takara) reaction using naive C57BL/6OlaHsd mice genomic DNA for the internal control or donor AAV plasmid for the Donor sample and purified using AMPure XP beads (Beckman Coulter ...