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Citations for Lonza :
4851 - 4900 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... A total of 5 × 105 cells were suspended in 100 μL of Nucleofector V solution mix (Lonza, VCA-1003) with 2 μg of GZMA and RASGRP1 CRISPR/Cas9 plasmids and subjected to nucleofection using the cell line-specific program T-O17 ...
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bioRxiv - Microbiology 2019Quote: ... HPCs were transfected using the Amaxa 4D system and the Primary Cell P3 solution according to the manufacturer’s instructions (Lonza). HPCs were transfected with 1ug pSIREN plasmid DNA per 106 cells using either program EH-100 or EO-100 ...
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bioRxiv - Genetics 2020Quote: Vero E6 cells were cultured in T-175 flasks in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, cat. 12-604F), supplemented with 8% fetal calf serum (FCS ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... The cell suspension was than centrifuged and the resulting pellet was resuspended in 5 ml DPBS (Lonza, Basel, Switzerland), thoroughly filtered through 40 μm filter (Corning Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... BEAS-2B was cultured in BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ (Lonza Catalog No. CC-3170). TALL-104 T cells were from the ATCC (ATCC® CRL-11386™ ...
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bioRxiv - Immunology 2021Quote: Growth medium for Rabbit Kidney (RK13) cells (CCL-37, ATCC) consisted of Dulbecco’s Minimum Essential Medium (12-614F, Lonza) supplemented to 10% fetal bovine serum (A3160401 ...
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bioRxiv - Bioengineering 2021Quote: ... These cells were then seeded at densities as above in ultra-low adherent round-bottom 96-well plates (Lonza) for 60 hours ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were free from mycoplasma contamination after testing with the MycoAlert™ PLUS mycoplasma detection kit (Lonza) (LT07-701).
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were nucleofected with both RNPs using the 4D-Nucleofector and P3 Primary Cell 4D-Nucleofector X Kit (Lonza). After 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells on filter sections were immobilized by embedding in 0.1% low-gelling agarose (MetaPhor™, Lonza, Rockland, ME, USA). CARD-FISH was performed using a HRP-labeled oligonucleotide probe specific for Nitrososphaeria (HRP-labeled Thaum726 [GCTTTCATCCCTCACCGTC] and unlabeled competitors [Thaum726_compA ...
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bioRxiv - Genomics 2021Quote: ... Human chronic myeloid leukemia HAP1 cells (Horizon™ C859) were cultivated in Iscove’s Modified Dulbecco’s Medium (IMDM) with 20% fetal bovine serum (Lonza) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... MSC used as control were passaged and maintained in MSCGM Mesenchymal Stem Cell Growth Medium BulletKitTM (PT-3001, Lonza). All steps were performed at 37°C.
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bioRxiv - Microbiology 2021Quote: ... In PRNT, Vero E6 cells (Vero C1008, ATCC CRL 1586) were cultured in Eagle’s minimal essential medium (EMEM, Lonza) supplemented with 5% FBS (GIBCO) ...
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bioRxiv - Immunology 2020Quote: ... Aliquots of 4 · 105 cells were transferred into individual wells of 16-well or 96-well nucleofection cuvettes (Lonza), combined with 20 µL pre-formed RNP or nucleofector solution (no RNP control) ...
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were cultured at 5% CO2/37°C in serum-free X-Vivo 15 medium (Lonza).
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bioRxiv - Immunology 2021Quote: ... using SF Cell line 4D-Nucleofector® X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection using electroporation was performed according to the manufacturer’s protocol using a P3 primary cell 4D-Nucleofector kit (Lonza) and program 138 for human keratinocytes ...
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bioRxiv - Microbiology 2021Quote: VeroE6 (ATCC® CRL 1586TM) wildtype and retrovirally-transduced cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... was passaged once in embryonated chicken eggs followed by two passages in Madin-Darby Canine Kidney (MDCK) cells (ATCC) in Eagle’s minimal essential medium (EMEM; Lonza) supplemented with 100 IU ml-1 penicillin-100 µg ml-1 streptomycin mixture (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: Tracheal cells (60,000-100,000 cells/ well in 96 well plate) were cultured in small airway growth media (SABMTMBasal Medium, CC-3119 Lonza) containing 1× insulin ...
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bioRxiv - Cancer Biology 2022Quote: ... Both B-ALL and CAR-T cells were prepared for transplantation by resuspending in 200 μL Hank’s balanced salt solution (Lonza) and loaded in 27.5-gauge syringes (Becton Dickinson) ...
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bioRxiv - Cell Biology 2022Quote: ... together with a single-stranded donor (ssODN) were delivered into mito-GFP1-10 cells using the Nucleofector system (Lonza). Cell line constitutively expressing mito-mCherry-GFP1-10 was generated by transducing ARPE-19 cells with lentiviral mito-mCherry-GFP1-10 and enriched for mCherry positive cells via cell sorting ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 μg ssODN and 1.2 μl 100 μM Alt-R Cas9 Electroporation Enhancer (IDT) were mixed and added to 20,000 cells resuspended in 18 μl SF Nucleofection Buffer (Lonza). Nucleofection mixture were transferred to 16-well Nucleocuvette strips (Lonza ...
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bioRxiv - Bioengineering 2022Quote: ... primary hepatocytes were seeded 400,000 cells per well in complete hepatocyte plating media (HCM kit, Lonza, Cat# CC-3198). After four hours of incubation at 37 °C and 5 % CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were mixed with complete p3 electroporation solution (from a Lonza 4D nucleofection kit; Lonza Group AG, Basel, Switzerland) followed by addition of the RNP complex and 500 pmoles of donor ssODNA (Alt-R HDR ...
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bioRxiv - Cell Biology 2022Quote: A sub-cloned population of BEL-A cells (1.0 × 105) were resuspended in CD34+ nucleofection kit buffer (Lonza Biosciences) containing 18 pmol Cas9 ...
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bioRxiv - Cell Biology 2022Quote: BEAS-2B cells (ATCC, Manassas, VA, USA) were cultured in Bronchial Epithelial Basal Medium (BEBM; Lonza, Walkersville, MD, USA) supplemented with the Bronchial Epithelial Growth Bullet Kit (BEGM ...
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bioRxiv - Immunology 2022Quote: ... PBMC were seeded at 1.5×106 cells per cm2 in X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) +1% penicillin-streptomycin (Cat ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: NIH-3T3 cells were grown in complete NIH-3T3 media (DMEM with 4.5g/L Glucose and 4mM L-Glutamine (Lonza), 10% ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells from a single donor were amplified and differentiated into air-liquid interface cultures as recommended by Lonza and described in (49) ...
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bioRxiv - Neuroscience 2023Quote: ... The two RNP complexes were then combined and diluted in 20 μL of P3 Primary Cell Nucleofector Solution (Lonza). To electroporate the Cas9 RNP complexes into hiPS cells ...
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bioRxiv - Neuroscience 2022Quote: Nucleofections were done on cortical neurons using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, LZ-V4XP-3024), on the day of preparation and dissociation (DIV 0) ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Systems Biology 2022Quote: DMECs were plated on poly-L-lysine-coated 6-well glass-bottom plates at 8,450 cells per cm2 in either BPM or growth medium (EGM2-MV, Lonza). After 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... once the cell confluency reached 90% the culture medium was changed to 10 mL XVIVO-10 medium (Lonza #(BE)BP04-743Q ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in 20 µL of SF Cell Line Nucleofector™ Solution with the supplement added (Lonza V4XC-2032). The cells and the RNP complex were carefully mixed and transferred to a well of the 16-well Nucleocuvette™ ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were nucleofected with the DT-130 program using the 4D-Nucleofector system with Core and X unit (Lonza). Nucleofected cells were incubated for 10min at room temperature before being transferred to a 6-well plate containing pre-warmed and equilibrated medium (DMEM + 10% FBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were screened every two months for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Basel, Switzerland). Passaged cells were continually monitored for viability using Countess cell counting slides (Thermo-Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Genetica Cell Line Testing) and cultures were regularly tested for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza). HEK293T and Calu-3 (ATCC-HTB-55 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were regularly authenticated by STR profiling (LabCorp, Burlington, NC) and tested for mycoplasma (MycoAlert, Lonza #LT07-218). To create a chronic CuHiexposure model ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cell lysis was performed for 5 min at room temperature in ACK lysing buffer (Lonza, #10-548E). For flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Neuroscience 2023Quote: ... We regularly monitored all cell cultures for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-218).
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bioRxiv - Biochemistry 2023Quote: Plasma cholesterol was depleted by treating HEK293 cells with 5 mM MβCD for 30 min in Pro293A-CDM (Lonza); this short period of MβCD treatment was deemed sufficient to removed 50% of endogenous cholesterol from cells (34) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were tested for mycoplasma at each batch freezing by PCR (32) and biochemical assay (MycoAlert, Lonza).
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bioRxiv - Cell Biology 2023Quote: ... after which the mixture was transferred to an electroporation cuvette and cells were electroporated in a 2b-Nucleofector (Lonza). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 cells were transfected with the PB-GFP-P2A-ΔNβCAT plasmid and a Piggybac transposase-coding plasmid using the P3 primary cell 4D nucleofector kit (Lonza). After 48 hours ...
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bioRxiv - Immunology 2024Quote: ... All cell lines were tested negative for mycoplasma contamination using the Mycoalert™ Mycoplasma Detection Kit (Lonza Bioscience Solutions) prior to use in functional assays.
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). When cells reach 80% confluency ...