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Citations for Lonza :
4701 - 4750 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: VeroE6 (ATCC® CRL 1586TM) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Lonza, Breda, the Netherlands) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were counted using Trypan Blue Exclusion Method and seeded into Collagen-I coated plates at a density of approximately 100,000 cells/cm2 following the Lonza’s instructions (Lonza, “Suspension and Plateable Cryopreserved Hepatocytes Technical Information and Instructions”) ...
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bioRxiv - Genomics 2022Quote: ... Wild type K562 cells were transfected with both the donor and guide plasmids by nucleofection (Lonza, program T016). Cells were selected 3 days after transfection with puromycin (2µg/mL ...
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bioRxiv - Immunology 2022Quote: ... Suspension cells were centrifuged to remove the culture medium and resuspended in 100 μL P3 nucleofection buffer (Lonza), to which 6.5 μL of RNP were mixed in ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs as described and cultivated overnight in X-Vivo 20 (Lonza, Basel, Switzerland) supplemented with 10% human serum ...
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bioRxiv - Cancer Biology 2022Quote: ... and MCa-P1362 cell lines were used in this study and tested to be mycoplasma free (MycoAlert-Lonza). Cells were cultured in vitro in Dulbecco’s modified Eagle’s medium (GIBCO ...
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bioRxiv - Immunology 2019Quote: ... Lifeact-GFP was transiently transfected into primary T cell blasts using the Amaxa 96-well shuttle system (Lonza) and the recommended protocol for activated human T cells.
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bioRxiv - Genomics 2019Quote: ... and 100 μM ssODN repair templates (180 bp, ssODN_Smarcc2_g1 or g2, IDT ultrameres) were nucleofected into ES cells using 4D-nucleofector (Lonza) for gene editing ...
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bioRxiv - Molecular Biology 2019Quote: ... Both cell lines tested negative for mycoplasma using a MycoAlert™ Mycoplasma Detection Kit (catalogue no. LT07; Lonza) and cultured in a humidified 5% CO2 atmosphere at 37 °C ...
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bioRxiv - Pathology 2020Quote: ... Plasmid delivery through electroporation of passage three neurospheres was performed using Mouse neural stem cell nucleofector kit (Lonza).
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bioRxiv - Microbiology 2020Quote: ... Nucleofection was performed using the program X-001 for Jurkat T-cells (Amaxa™ Nucleofector™ II, Lonza). Cells were cultured in RPMI-1640 medium media supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were washed once with PBS and then resuspended in 21.5μl of SF or P3 nucleofection buffer (Lonza), for K562s and HUDEP-2s/HSPCs ...
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bioRxiv - Bioengineering 2021Quote: ... All cell lines were tested for mycoplasma every 3 months using MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland). All cells used were <20 passages from thaw.
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bioRxiv - Biochemistry 2020Quote: Chinese hamster ovary DG-44 cells (Thermo Fischer Scientific) were cultured in the ProCHO 5 medium (Lonza, Switzerland), supplemented by 4 mM glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were confirmed mycoplasma-negative every two weeks and were last tested January 2019 (Lonza, # LT07-703). To maintain cellular phenotypes and to prevent cross-contamination of murine cell lines ...
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bioRxiv - Zoology 2020Quote: Leibovitz’s L-15 medium (HyClone) was diluted seven parts L-15 with three parts cell culture water (Lonza) to adjust for the osmolarity of amphibian cells and will herein be referred to as amphibian L-15 (AL-15) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells lines all tested negative for mycoplasma with the MycoAlter PLUS Mycoplasma Detection Kit (Lonza cat.#LT07-703). Lentivirus packaging was conducted at the Gene Vector and Virus Core of Stanford University or performed in-house using lentiviral constructs by co-transfection with ΔVPR ...
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bioRxiv - Genomics 2021Quote: ... Cells were regularly passaged and tested for presence of mycoplasma contamination with MycoAlert Plus Mycoplasma Detection Kit (Lonza).
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to 1×106 and transfected with 1,000ng of gRNA plasmid and 3,000 ng of pCMV_AncBE4max_P2A_GFP (Amaxa® Cell Line Nucleofector® Kit V, Lonza). K562 cells were cultured for an additional 48 hours then single clones of green cells were isolated using flow cytometry (Aria II) ...
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bioRxiv - Cell Biology 2021Quote: ... HMLER Snail-ER and HLMER-Snail (a gift from Robert Weinberg) cells were grown in 50% MEGM (Lonza) and 50% DMEM/F12 50:50 (Corning ...
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bioRxiv - Immunology 2021Quote: ... The virus was propagated in the human neuroblastoma cell line SK-N-SH in Eagle’s Minimum Essential Medium (EMEM) with Earle’s Balanced Salt Solution (EBSS) (Lonza), supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... using the Cell Line Nucelofector Kit V and the Amaxa Nucleofector II Device (Lonza Group AG, Basel, CH). Stable cells were selected for with 1mg/mL hygromycin B (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... EB3-mCherry construct (Stepanova et al., 2003) was transiently transfected using Amaxa Cell Line Nucleofector kit V (Lonza), program X-001.
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bioRxiv - Cell Biology 2020Quote: Jurkat T cells (clone E6.1; ATCC TIB-152) were grown in RPMI 1640 medium w/ L-Glutamine (Lonza) supplemented with 10% Fetal Bovine Serum and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Both cell lines were regularly tested for mycoplasma contamination by MycoAlert PLUS Mycoplasma Detection Kit (Lonza, LT07-703). REF52 cells were transiently transfected with mEos3.2-Actin expression vector (Michael W ...
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bioRxiv - Immunology 2020Quote: ... Vero E6 cells were obtained from the ATCC (ATCC CRL-1586) and cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 5% (FBS (EuroClone ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were passaged in poly-D-Lysine treated plates and incubated overnight in OPTI-MEM medium (Lonza) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... All cell lines were confirmed to be free of mycoplasmas using the MycoAlert detection kit (Lonza, Basel, Switzerland). Cells were detached in PBS EDTA 5mM without enzymatic solution to avoid HVEM cleavage.
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bioRxiv - Physiology 2022Quote: ... Media is removed and the cell pellet is resuspended in 3mL of ACK lysis buffer (Lonza 10-548E) for 2 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were negative for Mycoplasma upon regular testing with the MycoAlert Mycoplasma Detection Kit (Lonza; LT07-318). LARP1 knockout cell line generation was performed using Edit-R™ CRISPR-Cas9 Gene Engineering ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were routinely tested for mycoplasma contamination using MycoAlert PLUS mycoplasma detection kit (Lonza, MD, USA) and molecularly characterised using an “in house” panel of cellular and molecular markers to check that cell lines have not been cross contaminated (every 3-6 month) ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lines were regularly tested and verified to be mycoplasma negative using MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were regularly tested for mycoplasma contamination using a biochemical test kit (#LT07-318, Lonza, Switzerland) and were free of mycoplasma contamination.
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bioRxiv - Cell Biology 2022Quote: ... 25 μg mRNA was transfected into 2.5 × 106 freshly thawed fibroblasts using Cell Line Nucleofector Kit V (Lonza) and AMAXA program X-001 following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Transient transfections of these dominant-negative Rab11 a and siRab11 a into mouse MΦs (RAW 264.7 cell line) were performed with Amaxa mouse macrophage nucleofector kit (VPA-1009, Lonza) and DharmaFECT 4 Transfection Reagent (T-2004-01 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were washed with Dulbecco’s phosphate buffered saline without calcium or magnesium (DPBS-; Lonza, Walkersville, MD, USA) and detached with 0.25% trypsin with 0.53 mM EDTA (ATCC ...
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bioRxiv - Cell Biology 2022Quote: ... expression constructs were transfected into cells using the Amaxa™ 4D-Nucleofector (10μg plasmid cDNA; Lonza, Basel, Switzerland) or JetPrime (2μg plasmid cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were routinely tested for mycoplasma contamination by using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Microbiology 2022Quote: ... Both HeLa M and 293T cells and their derivatives were maintained in Dulbecco’s modified Eagle medium (DMEM) (Lonza) supplemented with 10% of fetal calf serum (FBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... and Jurkat cell lines tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza Cat #LT07-318).
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bioRxiv - Microbiology 2024Quote: ... Cells were then maintained in K-SFM (untreated) or switched to culture in Keratinocyte Basal Medium (KBM, Lonza) with CaCl2 added to a final concentration of 1.5 mM ...
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bioRxiv - Synthetic Biology 2024Quote: ... using SF Cell line 4D-Nucleofector X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely monitored for mycoplasma and confirmed negative at multiple times during this study using MycoAlert (Lonza). All cells were grown at 37°C with 5% CO2 in a humidified incubator.
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... and nucleofection was performed as per the manufacturer’s recommendations (Lonza, SG Cell Line 4D-Nucleofector X Kit S). Monoclonal isolation of the knockouts were performed by limiting dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... iMEPM cells were tested for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). Packaged lentiviruses containing pLV[shRNA]-EGFP:T2A:Puro-U6>Scramble_shRNA (vectorID ...
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bioRxiv - Neuroscience 2024Quote: ... we used the 4D-Nucleofector system and Amaxa P3 primary Cell 4D-Nucleofector X Kit S from Lonza. The electroporation buffer used was P3 primary cell Nucleofector Solution with Supplement 1 (Lonza) ...
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bioRxiv - Microbiology 2024Quote: ... Cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza catalog number LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Cancer Biology 2023Quote: K562 cells containing deletions in ALAS2 or FECH were created using a multi-guide strategy via nucleofection (Lonza) of Cas9/RNP complexes (Gene Knockout Kit v2 ...
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bioRxiv - Immunology 2024Quote: ... All recovered cell fractions were rested overnight in EP-TICLE culture media consisting of X Vivo-15 (Lonza) supplemented with 2% KnockOut serum replacement (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... All cell lines used were confirmed to be negative for mycoplasma by annual testing using MycoAlert Plus (Lonza). Last testing was performed on 14 August 2019.