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Citations for Lonza :
5101 - 5150 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: CRISPR–Cas9 gene knockout was performed by transient Cas9/gRNA (RNP) complex electroporation using the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). On day 4 of culture ...
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bioRxiv - Microbiology 2023Quote: BHK-21 cells (clone BSRT7/5) constitutively expressing the T7 RNA polymerase47 were grown in Dulbeco Modified Essential Medium (Lonza) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Biophysics 2023Quote: ... Directed differentiation of iPSCs into cardiomyocytes was triggered at 80–90% cell density by adding the RPMI 1640 medium (Lonza) containing B27 supplement minus insulin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 2μgs of the PARP1-sgRNAR-eGFP plasmid was nucleofected into the U2OS PARP1-/-and OVCAR8 PARP1-/-cell line using the Nucleofector (Lonza). Cells were selected in 2μg/mL G418 for 10 days and GFP-positive cells were sorted and used for subsequent experiments ...
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bioRxiv - Immunology 2023Quote: Hepatocytes (7×105 cells) were transfected with various plasmids (at 6 μM or indicated concentrations) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... the assembled ribonucleoprotein complexes were added to non-attached primary hepatocytes in suspension at 1x106 cells / mL in HCM media (Lonza). Cells were incubated with rocking at 37°C for 2 hours before transplantation ...
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bioRxiv - Cell Biology 2023Quote: ... This plasmid and plasmid pSPCas9(BB)-2A-puro(PX459)-hMyo10 above were transfected together into HeLa cells using an Amaxa nucleofection apparatus (Lonza). Five days later the cells were subjected to single-cell sorting into 96-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination using a MycoAlert PLUS Mycoplasma Detection Kit (#LT07-710, Lonza, Switzerland).
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bioRxiv - Genomics 2023Quote: ... 5 µg of the 4sgRNA plasmid or 10 µM synthetic guide RNAs were mixed and the cell/reagent/nucleofection mix was transferred to Nucleofection cuvette strips (Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Developmental Biology 2023Quote: ... 5.0-8.0 × 105 BTAG cells were electroporated with 3 μg of each gRNA using Amaxa 4D nucleofector (Lonza, V4XP-3012) and the CA-137 program ...
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bioRxiv - Bioengineering 2023Quote: ... iPSC and T cell electroporation were performed using the Amaxa™ 96-well Shuttle™ with the 4D Nucleofector (Lonza). Cas9 nucleases and sgRNAs were precomplexed in supplemented Nucleofector® Solution for 20 min at room temperature and the RNP solution was made up to a final volume of 2.5 µL (10X) ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa and SH-SY5Y cells were grown in Dulbecco’s modified Eagle medium with L-glutamine and 4.5 g/L glucose (DMEM; Lonza or Biowest) supplemented with 10% fetal calf serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5-2.0 x 105 cells were electroporated with 200 pmol of protein in 20 µl cuvettes using a 4D nucleofector device (Lonza) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... All sorted and parental cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza, LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Neuroscience 2023Quote: ... before being introduced to the KOLF2 hiPSCs through electroporation (P3 Primary Cell 4D-Nucleofector X Kit, Program CA-137, Lonza). The resulting clones were screened by multiplexed amplicon sequencing on an Illumina Mi-Seq platform with the MiSeq Reagent Kit V2 (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... An iCasp9-CD20/CD19-CAR transposon minicircle carrying CD20 scFv[17] and CD19 scFv[18] and a qPBase plasmid were delivered into activated T cells using 4D-Nucleofector device (Lonza) and Quantum Nufect Kit (GenomeFrontier Therapeutics ...
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bioRxiv - Bioengineering 2022Quote: ... B and T cell electroporations were performed using the Amaxa™ 96-well Shuttle™ with the 4D Nucleofector (Lonza). HSPC electroporations were performed in MaxCyte ATx ...
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bioRxiv - Biochemistry 2023Quote: ... All cell lines were regularly monitored and tested negative for the presence of mycoplasma using a commercial biochemical test (MycoAlert, Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... The cell lines were authenticated and regularly tested for Mycoplasma using the MycoAlert PLUS detection kit by Lonza (LT07-710).
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bioRxiv - Bioengineering 2023Quote: Approximately 0.25 × 106 U2OS.eGFP-PEST cells were nucleofected with 300 ng of Cas9 expression plasmid and 30 ng of eGFP-targeting gRNA expression plasmid using the SE Cell line 4D-Nucleofector X Kit (Lonza) and DN100 pulse program ...
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bioRxiv - Bioengineering 2023Quote: ... and 50 ng of AcrIIA4 expression or 300 ng of Cas9-P2A-CSD-AcrIIIA4 plasmid and 30 ng eGFP-targeting gRNA expression plasmid using the SE Cell line 4D-Nucleofector X Kit (Lonza) and DN100 pulse program ...
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bioRxiv - Cancer Biology 2022Quote: ... Kp27MYCER and B95-8) and adherent cell lines (HeLa, HEK293T) were grown in RPMI-1640 or DMEM basal media (Lonza) respectively supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... the Raji cells were transduced with mcDNA-CD19-CAR by using preset program DS104 of 4D-Nucleofector™ system (Lonza). Electroporation was performed according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... Samples of supernatant media from cell culture experiments were analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza).
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bioRxiv - Immunology 2023Quote: ... The volume of blood was determined and red blood cells were removed using ammonium-chloride-potassium (ACK) lysis buffer (Lonza) prior to antibody staining for flow cytometry analysis.
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were authenticated by Multiplex Cell Line Authentication (MCA) and were tested for mycoplasma by MycoAlert Mycoplasma Detection Kit (Lonza). U2OS cells were transfected with the plasmids using Lipofectamine 2000 and according to the manufacturer’s instructions (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were nucleofected with 4 µg of the sgRNA-containing plasmid individually following the Amaxa Mouse ES cell Nucleofector kit recommendations (VPH-1001, Lonza). Later ...
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bioRxiv - Systems Biology 2023Quote: ... The isolated CD14+ cells were cultured in Dulbecco′s Modified Eagle′s Medium (BE12-614F, DMEM, BioWhittaker, Lonza, Basel, Switzerland) supplemented with 10 % human AB+ serum (pooled from five healthy donors) ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lines used in this study were routinely tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (#LT07-318, Lonza), and mycoplasma-negative cells were used ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were nucleofected with RNPs using the 4D-Nucleofector and P3 Primary Cell 4D-Nucleofector X Kit and program CB150 (Lonza). After 24□hours ...
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bioRxiv - Genomics 2023Quote: ... purified Percoll-enriched mature schizonts (∼20 μl packed volume) were suspended in 100 μl of P3 primary cell solution (Lonza) containing 60 μg of linearized repair plasmid DNA (repair plasmid 1 and 2 separately in two separate transfection experiments ...
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bioRxiv - Synthetic Biology 2023Quote: ... An array of 20µL droplets of cell-laden hydrogel solutions were pipetted between two 25x75mm glass slides that were thrice treated with GelSlick (Lonza) separated by 3D-printed 400μm insert ...
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bioRxiv - Immunology 2023Quote: ... A total of 5×106 cells per transgene were washed with phosphate-buffered saline (PBS) and resuspended in 100µl NucleofectorTM Solution V (Lonza, Germany) containing the expression vector (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... pPB plasmids and pCMV-mPBase(neo-) encoding piggyBac transposase were co-transfected into MDCK cells by electroporation with an Amaxa nucleofector (Lonza). Cells were selected with the medium containing the following antibiotics ...
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bioRxiv - Biophysics 2023Quote: ... Samples of supernatant media from cell culture experiments were analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza).
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bioRxiv - Cancer Biology 2024Quote: ... Electroporation was performed with the Lonza SF Cell line 4D-Nucleofector X solution (#V4XC-2032) and the 4D-Nucleofector X Unit (Lonza) (#AAF-1003X ...
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bioRxiv - Systems Biology 2024Quote: The reporter cell line for the PTGR screen was created by nucleofection of haploid AN3-12 mESCs with 500ng of the reporter construct and 10 µg of a Tol2 transposase encoding plasmid using the Mouse ES Cell Nucleofector Kit (Lonza) according to the manufacturer’s protocol using an Amaxa Nucleofector (Lonza) ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC lines were validated by Array Comparative Genomic Hybridization assessment of karyotype and copy number variation (Cell Line Genetics) and confirmed to be sterile and pluripotent via MycoAlert testing (Lonza), Pluritest Array Analysis ...
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bioRxiv - Cancer Biology 2024Quote: CRISPR-mediated mutagenesis was carried out by transiently introducing CRISPR/Cas9 RNA-nucleoprotein complex into cultured NB4 cells with the 4D-Nucleofector system (Lonza). The sample preparation kit was Lonza SF Cell Line 4D-Nucleofector Kit S (Cat# V4XC-2024) ...
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bioRxiv - Neuroscience 2024Quote: ... Four wells of EBs per line were seeded in a 4-layer cell discs coated with growth factor-reduced matrigel in X-VIVO15 medium (Lonza) supplied with GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... To this end T cells were washed once in PBS and resuspended in complete P3 nucleofection buffer (Lonza, #V4XP-3032). 1e6 cells were then mixed with either Cas9 or base editor mRNA (4 - 4.5 µg ...
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bioRxiv - Cancer Biology 2023Quote: For the multi-ome analysis in young versus aged human bone marrow, bone marrow cells from young donors (26-year-old female, and 24-year-old male) were purchased from Lonza, while bone marrow samples from aged donors (70 and 77-year-old females ...
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bioRxiv - Biochemistry 2023Quote: ... 100pmol synthetic pegRNA (IDT) and 50pmol sgRNA (IDT) using the P3 primary cell nucleofector kit (cat#V4XP-3032, Lonza Biosciences) and program EH-115 on an Amaxa 4D-Nucleofector ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were propagated in Vero E6 cells, viral stocks and tissue culture supernatants were checked for endotoxin (Johnson et al., 2005) and mycoplasma (MycoAlert, Lonza) (La Linn et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting cells were transduced with the STAT regulatory reporter (4M67-GFP) (Wei et al., 2014) overnight in suspension in complete Mammary Epithelial Cell Growth Media (MEGM+) (Lonza, cat ...
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bioRxiv - Cell Biology 2023Quote: WT cells were transfected with the sgRNA-containing plasmids by electroporation with a Nucleofector device (Lonza; in Mirus transfection solution). Cells were plated in culture dishes and maintained for three days ...
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bioRxiv - Immunology 2023Quote: ... The transfection was carried out by nucleofection using Amaxa® Cell Line Nucleofector Kit V (Program X-001, Lonza, USA). At 24 h after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... we routinely checked cell cultures for the presence of mycoplasma using the MycoAlert™ Mycoplasma Detection Kit (#LT07-218, Lonza).
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bioRxiv - Microbiology 2023Quote: ... or 1×106 CEM-M7 Cas9 cells were transfected with the HiFi Cas9 Nuclease V3 (IDT)/gRNA complex (80 pmol/300 pmol) (Lonza) using a non-targeting or a GRN(5’-GCGATCCTGCTTCCAAAGATC-3’) ...
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bioRxiv - Cell Biology 2024Quote: ... with a density of 1.5 x106 using a 1:70 inoculation from the V1 stock for 92 hours at 21°C in Insect Xpress Protein-free Insect Cell Medium (Lonza) supplemented with GlutaMAX (GIBCO ...