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Citations for Lonza :
4751 - 4800 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Parasites and host cells were regularly tested for Mycoplasma contamination using the Mycoalert detection kit (Lonza; Basel, Switzerland). For all assays ...
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bioRxiv - Molecular Biology 2023Quote: ... All cell lines were frequently tested for mycoplasma using MycoAlert Plus Mycoplasma Detection Kit (cat# LT07-218, Lonza).
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: ... cells were kept in culture for 96 hours in complete hepatocyte basal medium (cat. no. CC-3198, Lonza). Cells were then fixed in 4% paraformaldehyde (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were tested negative for mycoplasma contamination with MycoAlert® Mycoplasma Detection Kit (Lonza, LT07-118).
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bioRxiv - Genetics 2023Quote: ... The cell suspension was transferred to the nucleofection cuvete and nucleofection was performed on the 4D-Nucleofector (Lonza) on the program for hESC ...
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bioRxiv - Cell Biology 2023Quote: ... Two days after transfection of WT HeLa cells with pSpCas9(BB)-2A-GFP-ghMyo10 using Amaxa nucleofection (Lonza), GFP-positive cells were subjected to single-cell sorting into 96-well plates using a BD FACS cell sorter ...
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bioRxiv - Molecular Biology 2023Quote: ... or Vero-hACE2-TMPRSS2 cells (Vero AT) (BEI NR-54970) in Eagle’s Minimum Essential Medium (Lonza, 12-125Q) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... The neutrophil pellet was then cleared of remaining red blood cells using ACK lysis buffer (Lonza #10-548E). For subsequent experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination by the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Molecular Biology 2023Quote: ... The RNP mixture was added to cells resuspended in nucleofection solution along with 0.5 ug pmax-GFP (Lonza) and 1 uL electroporation enhancer (100 uM ...
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bioRxiv - Developmental Biology 2023Quote: ... or pcDNA3.1(-) as a control using the AMAXA SG Cell line kit (4D-Nucleofector program EO-100; Lonza). Cells were further cultivated in DMEM/F12 supplemented with 10% FBS (Sigma ...
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bioRxiv - Immunology 2023Quote: ... 24-well plate) at a concentration of 75,000 cells per well in Smooth muscle growth medium (SmGM) (Lonza) and left to adhere and spread for 90 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-well dishes were seeded with 1.0 × 106 Sf9 cells per well in 2.0 mL InsectExpress medium (Lonza). Cells were transfected with 20 mL of bacmid per well ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were additionally screened for their sterility and the absence of mycoplasma during this process (LT07-703, Lonza).
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bioRxiv - Cell Biology 2023Quote: ... The RNP complex was mixed with confluent 2i-grown ES cells re-suspended in P3 transfection buffer (Lonza) before being transferred to an electroporation microcuvette well (Lonza) ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were cultured less than 3 months after resuscitation and tested for contaminants using MycoAlert (Lonza) every 1-3 months to ensure they were free of Mycoplasma contamination.
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bioRxiv - Neuroscience 2023Quote: ... CRISPR reagents were transfected into iPSCs using the P3 Primary Cell 4D Nucleofector™ X Kit S (Lonza), as previously described (Deneault et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended carefully in 20ul 4D-Nucleofector™ Solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza). Thereafter ...
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A spatially resolved EGFR signaling model predicts the length scale of GAB1-SHP2 complex persistencebioRxiv - Systems Biology 2023Quote: ... Cells were confirmed to be mycoplasma-negative using the MycoAlert Mycoplasma Detection Kit (LT07-318; Lonza, Basel, Switzerland).
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bioRxiv - Microbiology 2024Quote: ... Cells were then maintained in K-SFM (untreated) or switched to culture in Keratinocyte Basal Medium (KBM, Lonza) with CaCl2 added to a final concentration of 1.5 mM ...
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bioRxiv - Cancer Biology 2023Quote: K562 cells containing deletions in ALAS2 or FECH were created using a multi-guide strategy via nucleofection (Lonza) of Cas9/RNP complexes (Gene Knockout Kit v2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were stored in humidified incubators at 37 °C with 5% CO2 and routinely checked for mycoplasma (Lonza).
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bioRxiv - Bioengineering 2023Quote: ... The supernatant medium from cell cultures was analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza) or via polymerase chain reaction (PCR).
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bioRxiv - Biochemistry 2023Quote: ... Cell lines were tested once a month for mycoplasma contamination using Mycoalert® Detection Kit (Lonza, Basel, Switzerland). MCF7 and MDA-468 cells were grown in RPMI 1640 medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... T cells were subsequently transfected by electroporation of RNP using setting E0115 of the Nucleofector II device (Lonza). Cells were cultured overnight at density of 3×106 cells/mL in complete RPMI supplemented with 5 ng/mL IL-7 and IL-15 to account for cell loss after electroporation (∼33%) ...
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bioRxiv - Immunology 2023Quote: ... or KSQ-001 cells were thawed and rested in serum-free X-VIVO15 media (Lonza, Cat# 04-418Q) overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely monitored for mycoplasma and confirmed negative at multiple times during this study using MycoAlert (Lonza). All cells were grown at 37°C with 5% CO2 in a humidified incubator.
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... and nucleofection was performed as per the manufacturer’s recommendations (Lonza, SG Cell Line 4D-Nucleofector X Kit S). Monoclonal isolation of the knockouts were performed by limiting dilution ...
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bioRxiv - Synthetic Biology 2024Quote: ... using SF Cell line 4D-Nucleofector X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza catalog number LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Genetics 2024Quote: ... Daudi cells were then washed with PBS twice and resuspended in SF solution (Lonza, catalog number V4XC-2032), 300k cells per 20 µl solution ...
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bioRxiv - Cancer Biology 2024Quote: Bone marrow KLS cells were sorted from TauT+/ or TauT-/- mice and cultured overnight in X-VIVO15 (Lonza) media supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were electroporated using the Amaxa system with the nucleofection kit Cat No VCA-1003 from Lonza. Cells were FACS-sorted into 96-well plates to obtain single-cell derived colonies carrying the INDEL mutations ...
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bioRxiv - Immunology 2024Quote: ... The P14 cell/Cas9/RNP mixes were transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Bioengineering 2024Quote: ... Insect Drosophila melanogaster Schneider S2 cells were cultivated at 26°C in Insect-XPRESS media (Lonza, Basel, Switzerland) supplemented with 2 mM L-glutamine.
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bioRxiv - Bioengineering 2024Quote: ... in a strip format using SF Cell Line 4D-Nucleofector™ X Kit S (Lonza, Cat# V4XC-2032). Purified PEs and CODEs were complexed with either pegRNA or cpegRNA to form RNP at 50 pmol protein ...
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bioRxiv - Cancer Biology 2024Quote: ... Possible mycoplasma contamination was routinely monitored across all cell lines using the MycoAlert detection Kit (Lonza; LT07-318).
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bioRxiv - Cell Biology 2022Quote: ... HCFs were then cultured in supplemented serum-free media (SSFM; 1 mM sodium pyruvate (Lonza; Basel, Switzerland), 2mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... TetO-CDC6 mESCs were cultured on 0.1% gelatin-coated plates in Dulbecco’s modified Eagle’s medium (DMEM) with Ultraglutamine 1 and 4.5 g/L glucose (Lonza) supplemented with 15% FBS (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... After 1 h the medium was carefully replaces with supplemented hepatocyte maintenance medium (HMM) (#MP250, Lonza, Swizerland) until stimulations ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with 10% (v/v) fetal bovine serum (HyClone) and 1% (v/v) antibiotic antimycotic solution (Lonza). The cells were transfected with plasmid (pCMV6-entry from Origene ...
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bioRxiv - Biophysics 2019Quote: ... Approximately 1 to 1.5 × 106 hPSCs were washed in DPBS and resuspended in P3 primary buffer (Lonza) with 1 μg gRNA ...
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bioRxiv - Microbiology 2021Quote: ... SuBK12-08 and Tb 1 Lu in Eagle’s Minimum Essential Medium (EMEM, Lonza Cat. No: 12-662F) with 1% of L-Glutamine (200 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were centrifuged and the supernatant was aspirated and pellet was resuspended in the appropriate volume of P3 supplement buffer (20μl per condition by mixing 16.4μl P3 buffer and 3.6μl of supplement 1, Lonza, V4XP-3032). The cells were transferred into 16-well nucleovette strips (Lonza) ...
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bioRxiv - Genetics 2024Quote: ... RNP complexes together with the repair template were transfected into 1 million iPSCs with Amaxa nucleofector (Lonza, Human Stem Cell kit 2 ...
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bioRxiv - Genomics 2024Quote: ... All media was supplemented with 10% Fetal Bovine Serum (FB12999102, FisherScientific) and 1% Penicillin-Streptomycin (Lonza,17602E). Cells were cultured at 37℃ with 5% CO2 in 10 cm tissue culture dishes until confluent.
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bioRxiv - Developmental Biology 2021Quote: HEK293T/17 cells (ATCC CRL-11268) were grown in Dulbecco’s modified Eagle medium (Hyclone) supplemented with 10% fetal bovine serum (Lonza) and penicillin-streptomycin (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... All the cells used in this study tested negative for mycoplasma contamination using MycoAlert™ Mycoplasma detection kit (Lonza).
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bioRxiv - Molecular Biology 2021Quote: ... siRNA transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X kit L (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mixture was left on cells for 12-14 hours and viral production medium (Ultraculture, Lonza BioWhittaker 12-725F) supplemented with 1% Pen/Strep ...