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Citations for Lonza :
3701 - 3750 of 4418 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were nucleofected with 2μg of pcDNA3.1+ plasmid per reaction (Lonza-AMAXA program X-001, Nucleofector 2b). 24 hours after transfection ...
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bioRxiv - Microbiology 2021Quote: ... cells on filter sections were immobilized by embedding in 0.1% low-gelling agarose (MetaPhor™, Lonza, Rockland, ME, USA). CARD-FISH was performed using a HRP-labeled oligonucleotide probe specific for Nitrososphaeria (HRP-labeled Thaum726 [GCTTTCATCCCTCACCGTC] and unlabeled competitors [Thaum726_compA ...
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bioRxiv - Genomics 2021Quote: ... Human chronic myeloid leukemia HAP1 cells (Horizon™ C859) were cultivated in Iscove’s Modified Dulbecco’s Medium (IMDM) with 20% fetal bovine serum (Lonza) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... HESCs were transfected with a total of 4ug DNA in a ratio 2:3 (gRNAs:donor template) using the Amaxa P3 Primary Cell 4D-Nucleofector Kit (Lonza). Puromycin was added 3 days after electroporation at a concentration of 0.5ug/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Eriksson’s lab) and human osteosarcoma (U2OS) (ATCC HTB-96) cells were cultured in high-glucose DMEM (Lonza, BE12-119F), supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... In PRNT, Vero E6 cells (Vero C1008, ATCC CRL 1586) were cultured in Eagle’s minimal essential medium (EMEM, Lonza) supplemented with 5% FBS (GIBCO) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with PBS and 1 ml of fresh Hepatocyte Culture Medium (HCM) was added per well (Lonza). At 6 or 72 hpi ...
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bioRxiv - Immunology 2021Quote: ... washed in PBS by centrifugation for 5 min at 1300 rpm at 4°C and resuspended at 4.0 × 106 cells/ml in complete medium (IMDM 2 mM glutamax I supplemented with 8% heat-inactivated FCS (Lonza), 2% heat-inactivated chicken serum ...
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bioRxiv - Microbiology 2021Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Immunology 2020Quote: ... Aliquots of 4 · 105 cells were transferred into individual wells of 16-well or 96-well nucleofection cuvettes (Lonza), combined with 20 µL pre-formed RNP or nucleofector solution (no RNP control) ...
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were cultured at 5% CO2/37°C in serum-free X-Vivo 15 medium (Lonza).
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bioRxiv - Immunology 2021Quote: ... using SF Cell line 4D-Nucleofector® X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Microbiology 2021Quote: Primary human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Cat# CC-2540, male; Lonza; Donor 1) or derived directly from a patient at Cambridge University Hospitals NHS Trust (Research Ethics Committee Reference 19/SW/0152 ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection using electroporation was performed according to the manufacturer’s protocol using a P3 primary cell 4D-Nucleofector kit (Lonza) and program 138 for human keratinocytes ...
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bioRxiv - Microbiology 2021Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Microbiology 2021Quote: VeroE6 (ATCC® CRL 1586TM) wildtype and retrovirally-transduced cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... was passaged once in embryonated chicken eggs followed by two passages in Madin-Darby Canine Kidney (MDCK) cells (ATCC) in Eagle’s minimal essential medium (EMEM; Lonza) supplemented with 100 IU ml-1 penicillin-100 µg ml-1 streptomycin mixture (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: Human umbilical vein endothelial cells (HUVEC) were cultured between passage 3 and 6 in EGM-2 complete medium (Lonza)3,52–55 ...
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bioRxiv - Cancer Biology 2022Quote: ... Both B-ALL and CAR-T cells were prepared for transplantation by resuspending in 200 μL Hank’s balanced salt solution (Lonza) and loaded in 27.5-gauge syringes (Becton Dickinson) ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting vector was used to generate a D.mel-2 stable cell line adapted to grow in suspension in serum-free Insect-Xpress medium (Lonza). In brief ...
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bioRxiv - Cell Biology 2022Quote: ... together with a single-stranded donor (ssODN) were delivered into mito-GFP1-10 cells using the Nucleofector system (Lonza). Cell line constitutively expressing mito-mCherry-GFP1-10 was generated by transducing ARPE-19 cells with lentiviral mito-mCherry-GFP1-10 and enriched for mCherry positive cells via cell sorting ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 μg ssODN and 1.2 μl 100 μM Alt-R Cas9 Electroporation Enhancer (IDT) were mixed and added to 20,000 cells resuspended in 18 μl SF Nucleofection Buffer (Lonza). Nucleofection mixture were transferred to 16-well Nucleocuvette strips (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T cells (human embryonic kidney; LGC Nordic ATCC CRL-3216) were cultured in DMEM high glucose (#BE12-614Q, Lonza), supplemented with 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were mixed with complete p3 electroporation solution (from a Lonza 4D nucleofection kit; Lonza Group AG, Basel, Switzerland) followed by addition of the RNP complex and 500 pmoles of donor ssODNA (Alt-R HDR ...
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bioRxiv - Cell Biology 2022Quote: A sub-cloned population of BEL-A cells (1.0 × 105) were resuspended in CD34+ nucleofection kit buffer (Lonza Biosciences) containing 18 pmol Cas9 ...
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bioRxiv - Cell Biology 2022Quote: BEAS-2B cells (ATCC, Manassas, VA, USA) were cultured in Bronchial Epithelial Basal Medium (BEBM; Lonza, Walkersville, MD, USA) supplemented with the Bronchial Epithelial Growth Bullet Kit (BEGM ...
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bioRxiv - Microbiology 2022Quote: ... and neonatal dermal microvascular endothelial cells (hDMVEC) were maintained as monolayer cultures in EBM-2 medium (Lonza, Walkersville, USA) supplemented with 5% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells from a single donor were amplified and differentiated into air-liquid interface cultures as recommended by Lonza and described in (49) ...
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bioRxiv - Neuroscience 2023Quote: ... The two RNP complexes were then combined and diluted in 20 μL of P3 Primary Cell Nucleofector Solution (Lonza). To electroporate the Cas9 RNP complexes into hiPS cells ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cell lysis was performed for 5 min at room temperature in ACK lysing buffer (Lonza, #10-548E). For flow cytometry ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were regularly authenticated by STR profiling (LabCorp, Burlington, NC) and tested for mycoplasma (MycoAlert, Lonza #LT07-218). To create a chronic CuHiexposure model ...
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bioRxiv - Neuroscience 2022Quote: Nucleofections were done on cortical neurons using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, LZ-V4XP-3024), on the day of preparation and dissociation (DIV 0) ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Systems Biology 2022Quote: DMECs were plated on poly-L-lysine-coated 6-well glass-bottom plates at 8,450 cells per cm2 in either BPM or growth medium (EGM2-MV, Lonza). After 24 hours ...
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bioRxiv - Immunology 2022Quote: ... 1 x 106 T-cells were pelleted and re-suspended in 100 μL nucleofection solution (Lonza, Walkersville, MD, USA). T-cell suspensions were mixed with 5 μl RNP complex and cell/RNP mix transferred to Nucleofector cuvette ...
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). Once cells reached 80% confluency ...
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). When cells reach 80% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Umbilical Vein Endothelial Cells (HUVECs) were cultured in complete EGM-2 (cat# CC-3162, Lonza, Allendale, NJ, USA) and were not used beyond passage five for any experiments.
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X S kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Human HeLa cells (ATCC) were grown at 37°C in 5% CO2 using Dulbecco’s Modified Eagle Medium (Lonza, USA) supplemented with 10% FBS superior (Biochrom ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were screened every two months for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Basel, Switzerland). Passaged cells were continually monitored for viability using Countess cell counting slides (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were nucleofected with the DT-130 program using the 4D-Nucleofector system with Core and X unit (Lonza). Nucleofected cells were incubated for 10min at room temperature before being transferred to a 6-well plate containing pre-warmed and equilibrated medium (DMEM + 10% FBS) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were tested for mycoplasma at each batch freezing by PCR (32) and biochemical assay (MycoAlert, Lonza).
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bioRxiv - Cell Biology 2023Quote: ... after which the mixture was transferred to an electroporation cuvette and cells were electroporated in a 2b-Nucleofector (Lonza). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 cells were transfected with the PB-GFP-P2A-ΔNβCAT plasmid and a Piggybac transposase-coding plasmid using the P3 primary cell 4D nucleofector kit (Lonza). After 48 hours ...
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bioRxiv - Immunology 2024Quote: ... All cell lines were tested negative for mycoplasma contamination using the Mycoalert™ Mycoplasma Detection Kit (Lonza Bioscience Solutions) prior to use in functional assays.
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bioRxiv - Microbiology 2024Quote: ... Genetica Cell Line Testing) and cultures were regularly tested for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza). HEK293T and Calu-3 (ATCC-HTB-55 ...