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Citations for Lonza :
3501 - 3550 of 4418 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... spleen and lymph nodes were gently homogenized and red blood cells were lysed in ACK lysis buffer (Lonza). The remaining cells were washed by PBS with 2%FBS and filtered through with a 50ml falcon tube ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were seeded in 6 well plates and grown to ∼80% confluency in DMEM (Lonza, 12-614F) with P/S ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 6.6 % FCS and 33 % EBM™-2 Endothelial Cell Growth Basal Medium-2 (Lonza, Basel, Switzerland). HELA cells (DSMZ ACC-57 ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Microbiology 2020Quote: Electroporation was performed using the Amaxa P3 Primary Cell 96-well Nucleofector kit and 4D Nucleofector system (Lonza). Recombinant S ...
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bioRxiv - Neuroscience 2021Quote: ... freshly isolated cortical neurons were transfected using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, LZ-V4XP-3024) and the program DC-104 of the 4D-Nucleofector device (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: ... RNP was delivered by electroporation using a Lonza 4D-nucleofector and P3 Primary Cell Kit (Lonza, V4XP-3012). Cells were transferred to fresh StemSpan media and corresponding (AK2 or AAVS1 ...
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bioRxiv - Immunology 2019Quote: ... All Cell lines were Mycoplasma-free as determined with the MycoAlert mycoplasma detection kit (Lonza, Walkersville, MD, USA) and were carried no more than 20 passages from the validated stocks.
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bioRxiv - Microbiology 2021Quote: VeroE6 (ATCC® CRL 1586TM) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Lonza, Breda, the Netherlands) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... A human breast MCF10A cell line (CRL10317, ATCC) was grown with completed growth medium: MEGM Kit (Lonza CC3150) without gentamycin-amphotericin B mix (GA1000 ...
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bioRxiv - Neuroscience 2022Quote: ... were cultured in EGM-2 endothelial cell growth medium-2 from the BulletKit (Lonza, Basel, Switzerland; CC-3162). Pericytes and astrocytes were used for experiments up to passage 4 ...
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bioRxiv - Neuroscience 2022Quote: ... Human umbilical vein endothelial cells (HUVECs) were cultured in EBM-2 medium supplemented with EGM-2 SingleQuots (Lonza) on plates coated with rat tail collagen I (20 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were counted using Trypan Blue Exclusion Method and seeded into Collagen-I coated plates at a density of approximately 100,000 cells/cm2 following the Lonza’s instructions (Lonza, “Suspension and Plateable Cryopreserved Hepatocytes Technical Information and Instructions”) ...
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bioRxiv - Genomics 2022Quote: ... Wild type K562 cells were transfected with both the donor and guide plasmids by nucleofection (Lonza, program T016). Cells were selected 3 days after transfection with puromycin (2µg/mL ...
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bioRxiv - Immunology 2022Quote: ... 1 x 10^6 purified naive T-cells were resuspended in 15µL buffer P3 + 5µL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7µL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Immunology 2022Quote: ... Suspension cells were centrifuged to remove the culture medium and resuspended in 100 μL P3 nucleofection buffer (Lonza), to which 6.5 μL of RNP were mixed in ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs as described and cultivated overnight in X-Vivo 20 (Lonza, Basel, Switzerland) supplemented with 10% human serum ...
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bioRxiv - Cancer Biology 2022Quote: ... and MCa-P1362 cell lines were used in this study and tested to be mycoplasma free (MycoAlert-Lonza). Cells were cultured in vitro in Dulbecco’s modified Eagle’s medium (GIBCO ...
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bioRxiv - Immunology 2019Quote: ... Lifeact-GFP was transiently transfected into primary T cell blasts using the Amaxa 96-well shuttle system (Lonza) and the recommended protocol for activated human T cells.
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bioRxiv - Genomics 2019Quote: ... and 100 μM ssODN repair templates (180 bp, ssODN_Smarcc2_g1 or g2, IDT ultrameres) were nucleofected into ES cells using 4D-nucleofector (Lonza) for gene editing ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs P6 were cultured on flask in EGM™-2 Endothelial Cell Growth Medium-2 BulletKit ™ (Lonza). Cells were detached and loaded in PLA devices with seeding density 1 ×106 cells/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... Both cell lines tested negative for mycoplasma using a MycoAlert™ Mycoplasma Detection Kit (catalogue no. LT07; Lonza) and cultured in a humidified 5% CO2 atmosphere at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Nucleofection was performed using the program X-001 for Jurkat T-cells (Amaxa™ Nucleofector™ II, Lonza). Cells were cultured in RPMI-1640 medium media supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were washed once with PBS and then resuspended in 21.5μl of SF or P3 nucleofection buffer (Lonza), for K562s and HUDEP-2s/HSPCs ...
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bioRxiv - Bioengineering 2021Quote: ... All cell lines were tested for mycoplasma every 3 months using MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland). All cells used were <20 passages from thaw.
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bioRxiv - Microbiology 2021Quote: ... while HO-1-N-1 (JCRB0831) cells were cultured in DMEM/F-12 (Dulbecco’s Modified Eagle Medium:F12; Lonza) medium ...
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bioRxiv - Biochemistry 2020Quote: Chinese hamster ovary DG-44 cells (Thermo Fischer Scientific) were cultured in the ProCHO 5 medium (Lonza, Switzerland), supplemented by 4 mM glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were confirmed mycoplasma-negative every two weeks and were last tested January 2019 (Lonza, # LT07-703). To maintain cellular phenotypes and to prevent cross-contamination of murine cell lines ...
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bioRxiv - Zoology 2020Quote: Leibovitz’s L-15 medium (HyClone) was diluted seven parts L-15 with three parts cell culture water (Lonza) to adjust for the osmolarity of amphibian cells and will herein be referred to as amphibian L-15 (AL-15) ...
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bioRxiv - Bioengineering 2020Quote: ... Human umbilical vein endothelial cells (HUVECs) and normal human lung fibroblasts (NHLFs) were purchased from Lonza (Walkersville, MD) and used before passage 6 and passage 8 respectively ...
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bioRxiv - Bioengineering 2019Quote: ... or γδ-T cells were activated and cultured for 2 days at 0.5 to 1.0 million cells/mL in XVivo15 medium (Lonza) with 5% Fetal Bovine Serum ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells lines all tested negative for mycoplasma with the MycoAlter PLUS Mycoplasma Detection Kit (Lonza cat.#LT07-703). Lentivirus packaging was conducted at the Gene Vector and Virus Core of Stanford University or performed in-house using lentiviral constructs by co-transfection with ΔVPR ...
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bioRxiv - Genomics 2021Quote: ... Cells were regularly passaged and tested for presence of mycoplasma contamination with MycoAlert Plus Mycoplasma Detection Kit (Lonza).
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to 1×106 and transfected with 1,000ng of gRNA plasmid and 3,000 ng of pCMV_AncBE4max_P2A_GFP (Amaxa® Cell Line Nucleofector® Kit V, Lonza). K562 cells were cultured for an additional 48 hours then single clones of green cells were isolated using flow cytometry (Aria II) ...
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bioRxiv - Cell Biology 2021Quote: ... HMLER Snail-ER and HLMER-Snail (a gift from Robert Weinberg) cells were grown in 50% MEGM (Lonza) and 50% DMEM/F12 50:50 (Corning ...
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bioRxiv - Immunology 2021Quote: ... The virus was propagated in the human neuroblastoma cell line SK-N-SH in Eagle’s Minimum Essential Medium (EMEM) with Earle’s Balanced Salt Solution (EBSS) (Lonza), supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... using the Cell Line Nucelofector Kit V and the Amaxa Nucleofector II Device (Lonza Group AG, Basel, CH). Stable cells were selected for with 1mg/mL hygromycin B (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: A human breast MCF10A cell line (CRL10317, ATCC) was grown with completed growth medium: MEGM Kit (Lonza CC3150) without gentamycin-amphotericin B mix (GA1000 ...
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bioRxiv - Cell Biology 2020Quote: ... U–2–OS-pEP15 cells (5) were maintained in 1 mg/ml glucose phenol-red-free DMEM (Lonza) supplemented with steroid-free FBS (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... EB3-mCherry construct (Stepanova et al., 2003) was transiently transfected using Amaxa Cell Line Nucleofector kit V (Lonza), program X-001.
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bioRxiv - Cell Biology 2020Quote: Jurkat T cells (clone E6.1; ATCC TIB-152) were grown in RPMI 1640 medium w/ L-Glutamine (Lonza) supplemented with 10% Fetal Bovine Serum and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Both cell lines were regularly tested for mycoplasma contamination by MycoAlert PLUS Mycoplasma Detection Kit (Lonza, LT07-703). REF52 cells were transiently transfected with mEos3.2-Actin expression vector (Michael W ...
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bioRxiv - Bioengineering 2020Quote: Human bone marrow-derived mesenchymal stromal cells (MSCs) from a single donor were purchased from Lonza (Walkersville, MD). The trilineage potential of the cells was confirmed through induction in lineage-specific media ...
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bioRxiv - Microbiology 2021Quote: ... The human lung epithelial cell line Calu-3 (ATCC HTB-55) was maintained in DMEM F-12 (Lonza) supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Vero E6 cells were obtained from the ATCC (ATCC CRL-1586) and cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 5% (FBS (EuroClone ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... the siRNAs were transfected into human CD4+ T cells using the Amaxa Nucleofector following the manufacturer’s protocols (Lonza). The siRNAs used in this study were purchased from GenePharma and the sequences are as below:
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were passaged in poly-D-Lysine treated plates and incubated overnight in OPTI-MEM medium (Lonza) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... All cell lines were confirmed to be free of mycoplasmas using the MycoAlert detection kit (Lonza, Basel, Switzerland). Cells were detached in PBS EDTA 5mM without enzymatic solution to avoid HVEM cleavage.
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bioRxiv - Cancer Biology 2022Quote: ... All cells were negative for Mycoplasma upon regular testing with the MycoAlert Mycoplasma Detection Kit (Lonza; LT07-318). LARP1 knockout cell line generation was performed using Edit-R™ CRISPR-Cas9 Gene Engineering ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were routinely tested for mycoplasma contamination using MycoAlert PLUS mycoplasma detection kit (Lonza, MD, USA) and molecularly characterised using an “in house” panel of cellular and molecular markers to check that cell lines have not been cross contaminated (every 3-6 month) ...