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Citations for Lonza :
3651 - 3700 of 4418 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: The expression plasmids for the HA-V5-FLAG-dCas9 bait and the dCas9-VPR were delivered to J-Lat 11.1 cells by nucleofection using Amaxa Nucleofector (Lonza) as previously described (3 ...
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bioRxiv - Cancer Biology 2021Quote: MG-63 cells (1000 per well, 24-well plate) were seeded into 0.4% low melting point agarose (Lonza, 50101) on top of a 1% agarose layer ...
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bioRxiv - Microbiology 2020Quote: ... Stock titer was determined by plaque assay (described below) on HeLa cells overlaid with MEM (Lonza, Walkersville, MD, USA) + 0.5% agarose (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... EBs were collected and transferred into 6-well cell culture plate (12-16 EBs/well) in X-VIVO15 (Lonza) supplemented with IL-3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Vero cells were plated at 2.5 x 105 cell/well in 24-well plates in Essential-modified Eagle Medium (EMEM, Lonza) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: ... Infections for plaque assay in Vero E6 cells were performed in Eagle’s minimal essential medium with 25 mM HEPES (EMEM; Lonza) supplemented with 2% FCS ...
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bioRxiv - Cell Biology 2021Quote: Vero cells were transfected with 2 µg plasmid DNA in the Amaxa Nucleofector solution R (Lonza Bioscience, Rockville, MD), following the manufacturer’s instructions with program V-01 ...
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bioRxiv - Cell Biology 2021Quote: ... siRNA transfected primary XP186LV (XP-C patient cells) were serum-deprived for at least 24 hours in Ham’s F10 (Lonza) containing 0.5% FCS and antibiotics to arrest cells in G0 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 x 106 NSCs were mixed with 2.5 µg of corresponding DNA using Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) with CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Bioengineering 2022Quote: ... HUVECs were obtained from Lonza™ and cultured in Endothelial Cell Growth Medium-2 (EGM-2, PromoCell® C-22111 or Lonza™ CC-3162). Human primary pericytes were purchased from PromoCell® (PromoCell® ...
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bioRxiv - Neuroscience 2022Quote: ... The BBB working medium was prepared using EGM-2 Endothelial Cell Growth Medium-2 BulletKit (Lonza, cat. #CC-3162), which includes EBM-2 Endothelial basal medium and endothelial growth supplements hEGF ...
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bioRxiv - Immunology 2022Quote: Mammalian cells used in this study include BSC-40 (ATCC CRL-2761) (in Dulbecco minimal essential medium, DMEM, Lonza), healthy human periphery CD14+ monocytes (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... All cells were free of Mycoplasma contamination as confirmed by routine testing using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Microbiology 2022Quote: ... and resuspended in 100 μL nucleofector solution at a concentration of 1.5 x 106 cells/100 μl following the instructions of the Nucleofector Kit V (Lonza). In vitro transcribed RNA (5 μg ...
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bioRxiv - Immunology 2022Quote: ... and human monocytic leukemia cell line THP-1 (ATCC TIB-202) were cultured in RPMI-1640 (Lonza, 12-115F) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Mycoplasma contamination of the cells were checked monthly using the MycoAlert Plus Mycoplasma Detection Kit (cat# LT07-703) (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 million cells were transfected with 2μg of donor vector and 2μg of each AAVS1 ZFN expression plasmids using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza). Cells were seeded in E8 medium supplemented with 10µM ROCK Inhibitor Y-27632 (Selleckchem) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 000 to 100 000 cells per reaction was then resuspended in 20uL of P3 solution (Lonza, V4XP-3032) and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were maintained in culture for the indicated time in the following medium: X-VIVO 10 (Lonza, BE04-380Q) supplemented with 20% BIT 9500 Serum Substitute (StemCell technologies ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Microbiology 2020Quote: ... cells were nucleofected using Nucleofector SE solution and reagent into Nucleocuvette following manufacturer’s instructions using 4D-Nucleofector System (Lonza). The cell number and nucleic acid amounts are indicated in each figure legends ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were washed with RPMI before being re-suspended in Lonza electroporation solution from Kit C (Lonza, Basel, Switzerland) and electroporated using program W-001 using the Amaxa nucleofector (Lonza ...
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bioRxiv - Biochemistry 2021Quote: ... or ds-UM DNA oligos (IDT) (Table S4) were nucleofected into mouse ES cells using an Amaxa nucleofector (Lonza) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cells were tested for Mycoplasma contamination every 4 - 6 weeks and before each experiment (Mycoalert Mycoplasma Detection kit, Lonza).
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells were debeaded with an EasySep magnet (STEMCELL) prior to resuspension and electroporation at 0.5-1×106 cell mL−1 in P3 buffer (Lonza). Following electroporations with RNPs and HDRTs ...
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bioRxiv - Bioengineering 2021Quote: ... Approximately 1.5e6 K562 cells were electroporated in a final volume of 20 µL in a 16-well nucleocuvette strip (Lonza). For the T-cell experiments ...
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bioRxiv - Immunology 2022Quote: ... The solution was centrifuged at 400G for 5 minutes at 4°C and the cell pellet was resuspended in 1mL of Hank’s Balanced Salt Solution (HBSS; Lonza) containing 1% BSA (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell lines were authenticated and tested for mycoplasma contamination regularly using MycoAlert mycoplasma detection kit (Lonza, NJ, USA). The cumulative culture length of cells between thawing and use in this study was less than 20 passages.
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bioRxiv - Immunology 2022Quote: NIH3T3 WT fibroblast cells (3T3) and human CD40L-expressing 3T3 (Urashima et al., 1996) were cultured in IMDM (Lonza) containing 10% FCS (Bodinco) ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells were infected with viruses at an MOI of 0.01 and cultured in B-ALI growth medium (Lonza) at 33°C ...
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bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
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bioRxiv - Bioengineering 2019Quote: ... Transfection of transposon and transposase vectors was performed on day 2 with 0,5 µg transposon MC and 2 µg SB100X transposase RNA per 1×106 T cells on a 4D-Nucleofector according to the manufacturer’s instructions (Lonza). Transfected CD8+ and CD4+ T cells were expanded using the G-Rex culture system (Wilson-Wolf) ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were confirmed negative for Mycoplasma prior to the study using the MycoAlert Mycoplasma Detection Kit (Lonza) according to the manufacturer’s specifications.
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bioRxiv - Neuroscience 2019Quote: ... Cells tested negative for mycoplasma on a regular basis using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, #CABRLT07‒710). Cell identity was confirmed by short tandem repeat analysis (STR analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... 1,000,000 cells were nucleofected with 3.33 µg of SQT1665 and 1.66 µg of plasmid expressing gRNA using SE Cell Line 4D-NucleofectorTM X Kit L (Lonza) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... MD Cat# CC-2540) and maintained in Bronchial Epithelial Cell Growth Medium (BEGM) (Lonza, Walkersville, MD, Cat# CC-3170) with all provided supplements in the BulletKit ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the cell pellet was transfected with 1.0 μg of cheriff eGFP using a Nucleofector IIS (Lonza, Basel, Switzerland) and Amaxa Basic Neuron SCN Nucleofector Kit (VSPI—1003 ...
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bioRxiv - Immunology 2020Quote: ... All cell lines were tested and confirmed to be Mycoplasma and endotoxin-free using the MycoAlert Detection kit (Lonza) and the Endosafe-PTS system (Charles River Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: ... DNA (10 μg) was transfected into SmOx P9 cells using a 4D nucleofector system (Lonza Group AG, Basel, Switzerland) with program FI-115 ...
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bioRxiv - Molecular Biology 2019Quote: ... A total of 5 × 105 cells were suspended in 100 μL of Nucleofector V solution mix (Lonza, VCA-1003) with 2 μg of GZMA and RASGRP1 CRISPR/Cas9 plasmids and subjected to nucleofection using the cell line-specific program T-O17 ...
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bioRxiv - Cancer Biology 2020Quote: 1×105 cells were suspended in 1 ml of 0.33% select agar in small airway growth medium without EGF (Lonza) and plated on a bottom layer of 0.5% select agar in the same media in six-well dishes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells (1 × 106/10 cm dish) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; catalog no. 12-604F, Lonza) with 20% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... HPCs were transfected using the Amaxa 4D system and the Primary Cell P3 solution according to the manufacturer’s instructions (Lonza). HPCs were transfected with 1ug pSIREN plasmid DNA per 106 cells using either program EH-100 or EO-100 ...
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bioRxiv - Genetics 2020Quote: Vero E6 cells were cultured in T-175 flasks in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, cat. 12-604F), supplemented with 8% fetal calf serum (FCS ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... The cell suspension was than centrifuged and the resulting pellet was resuspended in 5 ml DPBS (Lonza, Basel, Switzerland), thoroughly filtered through 40 μm filter (Corning Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... BEAS-2B was cultured in BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™ (Lonza Catalog No. CC-3170). TALL-104 T cells were from the ATCC (ATCC® CRL-11386™ ...
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bioRxiv - Immunology 2021Quote: Growth medium for Rabbit Kidney (RK13) cells (CCL-37, ATCC) consisted of Dulbecco’s Minimum Essential Medium (12-614F, Lonza) supplemented to 10% fetal bovine serum (A3160401 ...
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bioRxiv - Bioengineering 2021Quote: ... These cells were then seeded at densities as above in ultra-low adherent round-bottom 96-well plates (Lonza) for 60 hours ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were free from mycoplasma contamination after testing with the MycoAlert™ PLUS mycoplasma detection kit (Lonza) (LT07-701).
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were nucleofected with both RNPs using the 4D-Nucleofector and P3 Primary Cell 4D-Nucleofector X Kit (Lonza). After 24 hours ...