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Citations for Lonza :
3901 - 3950 of 4418 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... hESCs were nucleofected with 2 μg of CRISPR and 3 μl ssODN (100 μm) using the P3 Primary Cell Kit L (Lonza). hESCs were then plated onto puromycin-resistant (DR4 ...
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bioRxiv - Bioengineering 2020Quote: DNA delivery was carried out on 20 μL volume format using Amaxa 4D-Nucleofector System and Cell line nucleofector solution SF (Lonza). Total amount of DNA electroporated was 3 μg (1.5 μg each plasmid in the case of using two plasmids at the same time) ...
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bioRxiv - Cell Biology 2019Quote: ... HUVE cells were cultured in endothelial growth medium supplemented with 5% fetal bovine serum (FBS) and growth factors (EGM-2; Lonza). HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2019Quote: ... In vitro transcribed RNA derived from wt or mutant pEAN900 was electroporated into BHK-21 cells using Amaxa Nucleofector technology (Lonza), as described previously (Beerens and Snijder ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated by CD3/CD28 Abs for 2 days and nuclofected with 100 μM RORC or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Amaxa, Lonza), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... pelleted at 350 xg for 10 minutes and resuspended in EBM-2 Basal Medium (CC-3156, Lonzs, Basel, Switzerland) supplemented with Microvascular Endothelial Cell Growth Medium-2 SingleQuots Supplements (CC-4147, Lonza) and 15% fetal bovine serum ...
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bioRxiv - Neuroscience 2020Quote: ... Effects of PI3K inhibition on Syk phosphorylation in mouse WT primary microglia was studied by plating 100 000 cells in 96-well plate in Dulbecco’s modified Eagle’s medium (DMEM, Lonza, Verviers, Belgium) supplemented with 2mM L-glutamine (Lonza ...
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bioRxiv - Systems Biology 2020Quote: ... Cell lines were confirmed to be mycoplasma-negative using the Mycoalert PLUS Mycoplasma detection kit (Lonza, Cat. No.: LT07-703).
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bioRxiv - Microbiology 2021Quote: ... Tightly synchronized schizonts were transfected using the Amaxa 4-D electroporator and P3 Primary Cell 4D Nucleofector X Kit L (Lonza) according to the protocol described by Moon et al ...
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bioRxiv - Immunology 2020Quote: 2×106 freshly isolated primary B cells were washed in PBS and resuspended in 20 μl P3 Primary Cell Nucleofector Solution buffer prepared with Supplement 1 buffer (Lonza) according to the manufacturer’s instructions (P3 Primary Cell 4D-Nucleofector X Kit S) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lung cancer cell lines (H3122, H2228, A549, H460, Calu-1, and H1437) were cultured in RPMI-1640 (Lonza, 15-1675) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: Human aortic endothelial cells (HAEC, CC-2535) and human skeletal myoblasts (HSMM-Muscle Myoblasts, CC-2580) were obtained from Lonza Company ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were maintained at 37 °C under 5% CO2 and the cell culture was routinely tested for Mycoplasma using a luminescence-based MycoAlert kit (Lonza).
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bioRxiv - Immunology 2019Quote: ... were generated by seeding 2 x 106 BM cells in a 100 mm non-tissue culture treated dish in RPMI 1640 (Lonza) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2021Quote: ... 10,000,000 lymphoblastoid cells were nucleofected with 60 µg of RTW3027 and 20 µg of plasmid expressing gRNA and mCherry using Cell Line NucleofectorTM Kit V (Lonza) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg RNA was electroporated into BHK-21 cells using an Amaxa nucleofector 2b (program A-031) and Nucleofection T solution kit (Lonza). Transfected BHK-21 cells were mixed in a 1:1 ratio with cells susceptible to CoV infection ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with pcDNA3 HA eIF4GI plasmid or control green fluorescent protein (GFP)-expressing plasmid (pMAX-GFP plasmid, Lonza). pcDNA3 HA eIF4GI (1–1599 ...
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bioRxiv - Neuroscience 2019Quote: ... of viral supernatants were used to infect E2T cells (5 x 105 cells/well in 6-well plate) for plaque assays and cells were maintained in culture medium containing 5% SeaPlaque agarose (Lonza) until plaques become visible at ∼12 days after infection ...
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bioRxiv - Genomics 2021Quote: ... were nucleofected with 2 µg of each sgRNA/Cas9 plasmid and 3 pmol or 30 pmol (for E14-STNΔTsixP) repair oligo (Supplementary Table 6) using the Lonza 4D-Nucleofector with the P3 Primary Cell 4D-NucleofectorTM kit (Lonza) and CP-106 (for TXΔXertP and E14-STNΔTsixP ...
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bioRxiv - Molecular Biology 2021Quote: ... Control pulmonary arterial smooth muscle cells were obtained from human lung tissues from normal donor lungs at the Cleveland Clinic (gift of Drs. Suzy Comhair and Serpil Erzurum) and from pooled primary cells from a commercial sources (Lonza).
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bioRxiv - Genetics 2020Quote: ... 2×105 UE control hPSCs were transfected with a pair of pSpCas9(BB)-2A-GFP constructs containing the specific sgRNAs (350 ng per construct) using P3 Primary Cell 4D-Nuclecfector X Kit (Lonza). The transfected cells were plated in Matrigel-coated dish and cultured for 2 days ...
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bioRxiv - Cell Biology 2019Quote: Mesenchymal stromal cells originating from the bone marrow of two healthy human donors were purchased from Lonza (Walkersville, MD, USA) and stored at −180°C in liquid nitrogen prior to experimentation ...
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bioRxiv - Cell Biology 2019Quote: ... 2 x 106 cells of MEFs were transfected with 1.2 nM of siRNAs using Amaxa Nucleofector II (Lonza, Basel, Switzerland) and MF 1 Nucleofector kit (Lonza ...
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bioRxiv - Immunology 2019Quote: ... HA-PLD1 or myc-PLD2 using Amaxa® Cell Line Nucleofector® Kit V reagent (cat. # VCA-1003, Lonza, Switzerland).
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bioRxiv - Molecular Biology 2019Quote: ... Primary HBECs (human bronchial epithelial cells) were grown in 2% oxygen using a 1:1 mixture of bronchial epithelial basal medium (BEBM; Lonza) and DMEM and supplemented with SingleQuot (Lonza ...
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bioRxiv - Immunology 2019Quote: ... The U937 cells were electroporated using Lonza Nucleofector 2b (program W-001) and the Nucleofection Kit C (Lonza, VPA-1004). After electroporation ...
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bioRxiv - Immunology 2019Quote: ... A20 and A20 D1.3 B cells (3 × 106cells) were transiently transfected using the AMAXA nucleofector kit V (Lonza, #VCA-1003) or the Ingenio electroporation kit (Mirus ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µg of plasmids were mixed with 5 millions ESC in buffer “Mouse ES cell nucleofector kit (Lonza, VPH-1001) per electroporation ...
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bioRxiv - Genetics 2021Quote: ... were transfected with either KMT2D CRISPR-Cas9 vector or CRISPR-Cas9 empty vector together with GFP plasmid using P3 Primary Cell 4D-Nucleofector X kit (V4XP-3024, Lonza). Three days after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 25,000 HMVEC and 50,000 Calu-3 cells were seeded per insert on either the apical or basolateral side as indicated and grown in EGM-Plus (Lonza), EGM-2MV (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... HeLa (ATCC® CCL-2™), and HaCaT cells (gift from Jonathan Garlick, Tufts University) were grown in Dulbecco’s modified Eagle medium (DMEM; Lonza) containing high glucose ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were authenticated by Multiplex Cell Line Authentication (MCA) and were tested for mycoplasma by MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cell Biology 2019Quote: HMEC FL2 cell line was cultured as previously described (Katajisto et al. 2015) in Mammary epithelial basal medium (MEGM; Lonza). For Laminin preadhesion assays ...
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bioRxiv - Cell Biology 2020Quote: ... and 8 × 105 cells were electroporated using 5 mg of total DNA (Ratio 1:1) and the Amaxa Nucleofector kit (Lonza) as described (Ran et al. ...
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bioRxiv - Cancer Biology 2021Quote: Human A549 lung adenocarcinoma cells obtained from ATCC (Manassas VA) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM – Lonza, Wakersville MD) supplemented with 10% FBS superior (Biochrom ...
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bioRxiv - Microbiology 2021Quote: ... 2 µg pcDNA-pp71-flag and 1 µg of pBRep-Cre (Wolfram Brune, Hamburg, Germany) was electroporated into Fi301 cells using the Amaxa nucleofector program A-23 (Lonza). Plaque formation was checked over the course of 14 days ...
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bioRxiv - Microbiology 2021Quote: HEK293T-ACE2 cells (NR-52511, BEI Resources, Manasas, VA) were grown in Dulbecco’s Minimal Essential Medium (DMEM, Lonza, Walkersville, MD), supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: MCF10A cell line was obtained from American Type Culture Collection (ATCC) and cultured in mammary epithelial cell growth basal medium with single quots supplements and growth factors (#Lonza CC-4136). Cells were treated with the medium-chain fatty acids (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Ker-CT cells were maintained in KGM-Gold Keratinocyte growth medium supplemented with KGM-Gold™ BulletKit™(Lonza #00192060). Cells were split when confluent ...
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bioRxiv - Biophysics 2021Quote: ... 2 μg of each Pcdh expression construct were transfected into 20 μL of the K562 cell suspension by electroporation using an Amaxa 4D- Nucleofector (Lonza). Transfected cells were transferred to a 24-well plate in 500 μL of medium per well and incubated overnight at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Guide RNA vectors were nucleofected into the LaminB CRISPRi iPSC line using a P3 Primary Cell 96-well NucleofectorTM Kit (Lonza) and the 4D Nucleofector X Unit (Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... Stable LBH-overexpressing BT549 cell lines were generated by nucleofection of 2×106 BT549 cells with 2 μg linearized pCDNA3 or pCDNA3+Lbh 13 and 1 μg pEGFP (Lonza) in Solution V (Lonza) ...
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bioRxiv - Cancer Biology 2020Quote: ... with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin and EGM-2 Medium (EBM-2 Basal Medium with EGMTM-2 MV Microvascular Endothelial Cell Growth Growth Factor, Lonza) respectively for 18 hours before starting the co-culture of the two cell lines ...
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bioRxiv - Genetics 2019Quote: ... They were then transfected by nucleofection with 5 μg DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza) using the C-016 program (Amaxa Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: Tumour cells were genetically edited by electroporating the Cas9/crRNA/tracrRNA or Cas9/sgRNA ribonucleoprotein (RNP) complexes into cells using the 4D-nucleofector system (Lonza). To prepare the guide RNA complex ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg of RNA was electroporated into 5×106 BHK-21 cells using the Amaxa nucleofector 2b (program A-031) and Nucleofection T solution kit (Lonza). Transfected BHK-21 cells were mixed with HuH7 or Vero cells in a 1:1 ratio and plated for harvesting supernatants ...
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bioRxiv - Immunology 2021Quote: ... Cells were then nucleofected using a 4D-Nucleofector™ with X-Unit (V4XP-4032 and V4XP-9096, Lonza, Basel, Switzerland). Up to three RNP complexes in 9 µL were used per reaction ...
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bioRxiv - Immunology 2021Quote: ... The enriched CD4+CD25hi cells were cultured in 24-well non-tissue culture plates at 1 x 106 cells/mL in X-Vivo-15 (Lonza) supplemented with 10% heat-inactivated human AB serum (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 (500 ng/mL) at a density of 2 × 106 cells/mL of X-vivo 15 serum-free medium (Lonza). For iTreg differentiation ...
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bioRxiv - Immunology 2021Quote: A single-cell suspension was prepared from spleen and lymph nodes and red blood cells were removed by lysis with ACK buffer (Lonza). CD4+ T cells were enriched using microbeads (Miltenyi Biotec ...