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Citations for Lonza :
1351 - 1400 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The SF cell line 4D-Nucleofector X Kit S (V4XC-2032) from Lonza was used to prepare a mix of 300 pmol of gRNA and 100 pmol of Cas9 protein ...
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bioRxiv - Immunology 2024Quote: ... and embedded in 6% w/v low melting point agarose (Lonza) in 1x PBS ...
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bioRxiv - Molecular Biology 2024Quote: Human Aortic VSMCs (Lonza Cat No CC-2571 batch no: 21LT316170) were placed in 6 well places ...
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bioRxiv - Molecular Biology 2024Quote: ... SmGM-2 Smooth Muscle Cell Growth (Lonza Cat No. CC 3182) was used for incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... primary human foreskin keratinocytes (HFK) (Lonza, Basel, Switzerland), and HPV-18 E6/E7 immortalized HFKs (HFK18E6E7 ...
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bioRxiv - Immunology 2024Quote: Nucleofection of Jurkat cells was performed using SE Cell Line 4D-Nucleofector™ X Kit L (Lonza, cat. V4XC-1012), while the P3 Primary Cell 4D-Nucleofector™ X Kit L (Lonza ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in 2 mL of culturing media (Lonza X-VIVO-15 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 8 mM Glutamine (Cultek, Madrid, Spain) and Gentamicine Sulfate (0.25 mg/mL) (Lonza). The cultures were started in a volume of 400 mL of basal medium at a cell density of 350,000-400,000 cells/mL and were supplemented with Cell Boost 7a (Hyclone ...
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bioRxiv - Immunology 2024Quote: ... Plaque assay media was composed of 1X EMEM (Lonza # 12–684F) supplemented with 2% Heat Inactivated FBS (Gemini Biosciences #100–106) ...
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bioRxiv - Neuroscience 2024Quote: ... following a previously described protocol.43-44 Cultures underwent testing every other month using the MycoAlert Mycoplasma Detection Kit (Lonza LT07-318) and consistently tested negative for mycoplasma throughout the study.
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% (v/v) NP40) followed by one last wash with molecular grade water (Lonza). The beads were then subjected to RNase digestion in a 25 ul reaction containing 10mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were collected by centrifugation and washed twice using phosphate-buffered saline (PBS; without Ca, Mg; pH 7.3–7.5; Lonza, Basel, Switzerland). Ten-fold serial dilutions were made and 5µl of each dilution was spotted onto YPD agar plates ...
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bioRxiv - Microbiology 2024Quote: ... 1% v/v penicillin/streptomycin (17-745E, Lonza) and 1% v/v Glutamax™ at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
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bioRxiv - Cell Biology 2024Quote: ... In microscopy experiments we used the same media but without phenol red to reduce background fluorescence (Lonza CC-3153 phenol-red free basal media supplemented with growth factors and other components from the Lonza CC4136 kit). T98G cells were purchased from ATCC ...
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bioRxiv - Cell Biology 2024Quote: ... In microscopy experiments we used the same media but without phenol red to reduce background fluorescence (Lonza CC-3153 phenol-red free basal media supplemented with growth factors and other components from the Lonza CC4136 kit) ...
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bioRxiv - Cell Biology 2024Quote: ... Ligase 4 was transiently complemented into KO cell lines by transfecting 500,000 cells with 100 ng of pmaxGFP (Lonza) and 500 ng of expression plasmid (empty vector ...
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bioRxiv - Cancer Biology 2024Quote: ... in X-Vivo 15 media (Lonza, BE02-053Q) supplemented with 5% human AB serum (Millipore Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Collected schizonts were washed and then re-suspended in P3 buffer (Lonza) and mixed with DNA ...
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bioRxiv - Microbiology 2024Quote: ... stained with 1x Sybr green (Lonza) for 30 minutes in the dark ...
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bioRxiv - Microbiology 2024Quote: ... we counted cells with flow cytometry after staining with 1x Sybr green (Lonza 50513) for 30 minutes in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... in a specimen cup containing a custom-built tissue carrier and a small volume of keratinocyte serum-free medium (Lonza) to provide protection and physiologically relevant hydration ...
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bioRxiv - Molecular Biology 2024Quote: HUVECs and BAECs were cultured in EGM-2 (Lonza, Basel, Switherlad). Cells with fewer than five passages were used in the experiments ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mg Cas9-gRNA-2 plasmid and 1 mg eGFP-puromycin resistance plasmid using the Amaxa 4D-Nucleofector system (Lonza) according to the manufacturer’s instructions with a pulse load of CA137 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 mM sodium pyruvate (Lonza). Jurkat T cells were cultured in complete RPMI (Cytvia ...
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bioRxiv - Synthetic Biology 2024Quote: ... complete T cell media consisted of X-VIVO 15 (Lonza) supplemented with 5% human Ab serum and 55 µM 2-mercaptoethanol ...
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bioRxiv - Physiology 2024Quote: ... counted and 3.106 cells resuspended in 100 μl Nucleofactor R solution and electroporated with 3 µg of plasmid (pcDNA3 containing or not α7-5HT3 cDNA) using the Amaxa nucleofactor kit R (Lonza) according to the manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: ... were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience as previously described (3) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 6-well plates in EGM-2 medium (Lonza).
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bioRxiv - Immunology 2024Quote: ... One million NK cells were resuspended in 20 ul 4D nucleofector master mix (82% P3 + 18% supplement 1; Lonza, V4XP-3032), and mixed with Cas9 RNP for electroporation using program CM137 ...
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bioRxiv - Genomics 2024Quote: PBMCs were quickly thawed and placed in pre-warmed xVIVO15 cell culture medium (Lonza) supplemented with 5% heat-inactivated FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... freshly harvested spleens of 12-13 weeks-old female NOD mice with early diabetes were filtered through a nylon mesh by followed lysis of the red blood cells with ACK Lysing buffer (Lonza). After starvation ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant riboprotein complex was introduced into cells by nucleofection (4D-Nucleofector X unit, Lonza bioscience), using the SF cell line kit and A549 cell program (CM 130) ...
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bioRxiv - Neuroscience 2023Quote: ... The two RNP complexes were then combined and diluted in 20 μL of P3 Primary Cell Nucleofector Solution (Lonza). To electroporate the Cas9 RNP complexes into hiPS cells ...
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bioRxiv - Neuroscience 2023Quote: ... 500,000 cells were mixed with the nucleofection solution containing the RNPs and were electroporated in one well of a 16-well Nucleocuvette Strip using the program CA137 in 4D Nucleofector system (Lonza). Following electroporation ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y neurons (ATCC) were cultured in a 1:1 mixture of Eagle’s Minimum Essential Medium (EMEM, Lonza BioWhittaker) and F12 Medium (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... one million human iPSCs from the WTC-11 donor line (Coriell Institute for Medical Research, GM25256) at passage 37 were electroporated using the 4D-Nucleofector System (Lonza, AAF-1003X, V4XP-3024) with a preassembled complex of 10µg of S.p ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with neuronal survival factor-1 (NSF-1) (Lonza, Charles City, IA, USA, Cat# CC-4323), N2 supplement (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... using the 4D-Nucleofector system (Lonza), in combination with the P3 Primary Cell 4D-Nucleofector X kit ...
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bioRxiv - Molecular Biology 2023Quote: ... All cell lines were routinely tested for Mycoplasma contamination with the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, Switzerland, LT07-705) and found to be negative.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell lines were routinely tested for Mycoplasma and were negative (Lonza MycoAlert). Cells were cultured by standard methods in RPMI 1640 (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1% antibiotics (10000 U/ml penicillin, 10000 µg/ml streptomycin, Lonza). The cells were incubated in a humidified atmosphere at 37°C with 5% CO2.
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bioRxiv - Microbiology 2023Quote: ... T ransfections of BF cell lines with the Amaxa Nucleofector (Lonza) were carried out as described (20) ...
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bioRxiv - Microbiology 2023Quote: Normal Human Neonatal Dermal Fibroblasts (NHDF; Lonza, Basel, Switzerland) and monkey kidney epithelial cells (LLC-MK2 ...
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bioRxiv - Microbiology 2023Quote: ... and placed into a sterile 2 mm gap cuvette with the appropriate DNA and transfected using an Amaxa Nucleofector II (Lonza, Basel, Switzerland; U-33 program). Parasites were immediately transferred to LIT medium for 24 hours before adding 10 μg/mL puromycin (Invivogen ...
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bioRxiv - Microbiology 2023Quote: ... 190 μL was mixed with 10 μL of 20X SYBER Green I nucleic acid stain (Lonza Walkersville Inc, Walkersville, MD) in a well of a transparent 96-well plate and incubated in the dark for 15 minutes before being placed in the Guava easyCyte HT flow cytometer (488 nm ...
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bioRxiv - Neuroscience 2023Quote: ... Nucleofection was performed using the 4-D nucleofector system (AMAXA) and the P3 Primary Cell 4D-Nucleofector Kit (Lonza, V4XP-3024) following manufactures’ instructions (program CB-150) ...
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bioRxiv - Physiology 2023Quote: Human PAECs were cultured in complete EGM-2MV media (Lonza) in a cell culture incubator at 37°C with 5% CO2 and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: ... for 20h and fibroblasts cells were nucleofected using the SE Cell Line AD-Nucleofector Kit (Lonza). Knock-down procedures were performed with siRNA purchased from Riboxx (non-targeting siRNA ...
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bioRxiv - Neuroscience 2023Quote: ... CSF cells were resuspended in 5ml of X-Vivo15 media (Lonza). A total of 5 μl of the single-cell suspension were manually counted in a counting chamber ...