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Citations for Lonza :
1601 - 1650 of 9698 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... supplemented with 5% fetal bovine serum (Lonza, Basel, Switzerland) at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Physiology 2024Quote: ... the media was replaced with HCMTM Hepatocyte Culture Medium BulletkitTM (Cat # CC-3198, Lonza) except HEGF ...
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bioRxiv - Physiology 2024Quote: ... These cells were cultured in complete endothelial cell growth medium-2 (EGM-2) supplemented with the Bullet Kit (Lonza Biologics, Basel, Switzerland) and a 1% v/v penicillin-streptomycin antibiotics cocktail ...
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bioRxiv - Physiology 2024Quote: ... Gastric SMCs were routinely screened for mycoplasm contamination using the MycoAlert® Detection Kit (Lonza).
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bioRxiv - Physiology 2024Quote: ... Red blood cells were lysed in 2 ml of sterile ammonium–chloride–potassium lysis buffer (ACK Lysing Buffer, Lonza) for 5 minutes on ice ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... normal human lung fibroblasts (NHLF, CC-2512, Lonza) were stimulated with TGFβ for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: Human fibroblasts were seeded in 12-well plates in adipogenesis differentiation medium consisting of ɑMEM (Lonza, Geleen, The Netherlands) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... HBMEC were transfected with lentivirus or siRNA in EGM-2 media (Lonza). HeLa (ATCC CCL-2 ...
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bioRxiv - Neuroscience 2024Quote: Human brain microvascular endothelial cells (HBMEC) were purchased from SicenCell (#1000) and cultured in Endothelial Cell Growth Medium-2 media (EGM-2) (Lonza). HBMEC were transfected with lentivirus or siRNA in EGM-2 media (Lonza) ...
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bioRxiv - Pathology 2024Quote: ... ascorbic acid and heparin (EGM-2 SingleQuots Supplements, Lonza) medium without VEGF and reduced FBS (2% ...
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bioRxiv - Pathology 2024Quote: ... in Endothelial Basal Medium (EBM-2, Lonza) supplemented with hydrocortisone ...
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bioRxiv - Pathology 2024Quote: ... All cells were routinely tested for mycoplasma contamination using MycoAlert detection kit (Lonza). All cells were used within 5 years of receipt.
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bioRxiv - Evolutionary Biology 2024Quote: ... 4 µL SpCas9/gRNA RNP + 2 µL repair template (2 to 8 µg) + 2 µL primed cells (4E6 cells) + 10 µg pUC19 plasmid (used as carrier DNA) + SF buffer (Lonza) to a final volume of 24 µL ...
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bioRxiv - Pathology 2024Quote: ... Series of transfections in the murine hybridoma/myeloma cell line SP2/0 (ATCC® CRL-1581™) were performed using Cell line Nucleofactor kit V (Amaxa/Lonza, Basel, Switzerland) and a Nucleofactor II device (Amaxa/Lonza ...
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bioRxiv - Pathology 2024Quote: ... and a Nucleofactor II device (Amaxa/Lonza, Basel, Switzerland). Positive clones were then selected using neomycin (1mg/mL ...
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bioRxiv - Cell Biology 2024Quote: RPE1 TP53-/- cells stably expressing GFP-centrin (Wang et al., 2017) were electroporated in a home-made electroporation buffer (Zhang et al., 2014) using Amaxa Nucleofector II (Lonza). Cells were electroporated with an equal mix of two RNPs ...
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bioRxiv - Neuroscience 2024Quote: Human bone marrow MSCs (Lonza, Cat #PT-2501) were cultured in DMEM/F12 medium ...
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bioRxiv - Neuroscience 2024Quote: ... using Nucleofector 2b (Lonza; program A-023).
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bioRxiv - Neuroscience 2024Quote: ... were transfected in to ∼2x106 human sample fibroblasts by Amaxa Nucleofector (Lonza; program U-023) at a concentration of 2 µg/100 ml electroporation solution ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.2% MycoZap plus-PR (VZA2021, Lonza). After 10 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffered saline solution (CC-5024, Lonza) before renewing the medium or passaging the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell passaging was performed with 0.05% (w/v) trypsin solution (#204-16935, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and trypsin neutralizing solution (CC-5002, Lonza). To pellet the cells during the cell passaging ...
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bioRxiv - Molecular Biology 2024Quote: Primary human RPTECs (CC-2553, Lonza, Walkersville, MD, USA) were used for in vitro studies instead of immortalized cell lines because immortalized cell lines could potentially have fundamental differences in cellular metabolism compared with primary cells ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were produced using baculoviral infection of Trichoplusia ni cells cultured in Insect-XPRESS (Lonza, BP12-730Q) or ESF 921 (Expression Systems ...
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bioRxiv - Cell Biology 2024Quote: ... two Addgene vectors #73500 and #113194 were co-electroporated into hPSCs at 1:1 weight ratio using 4D-Nucelofector (Lonza Bioscience, Germany) at program CA137 ...
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bioRxiv - Cancer Biology 2024Quote: ... were incubated at 37°C/5%CO2 and supplemented in 10% FBS and EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, Switzerland). HUVECs and all other cancer cell lines were sub-cultured as adherent cells every 2-3 days.
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bioRxiv - Molecular Biology 2024Quote: ... for TC-NER UDS siRNA transfected primary XP186LV fibroblasts (XP-C patient cells) were seeded on 24 mm coverslips and serum-deprived for at least 24 hours in Ham’s F10 (Lonza) containing 0.25% FCS to arrest cells in G0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lines were routinely monitored for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Molecular Biology 2024Quote: ... 1.5x MOPS buffer (Lonza) denaturing gel ...
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bioRxiv - Molecular Biology 2024Quote: ... and electroporation was carried out using the Amaxa 2B-Nucleofector system (Lonza). Cells were replated at a density of 105 cells/cm2.
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bioRxiv - Molecular Biology 2024Quote: ... 1 % penicillin-streptomycin (PenStrep, Lonza), at 37 °C in an atmosphere of 5 % CO2/95 % air ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then suspended in buffer SE and transfected using 4D-Nucleofector (Lonza) program EN-113 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.8X MOPS Buffer (Lonza) denaturing gel and electrophoresed ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5x MOPS Buffer (Lonza) denaturing gel ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were washed with DPBS (Lonza, # 17-515F) via centrifugation at 1500 × g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Bxb1 plasmid and cargo donor containing the attB site in iPSCs (200000 cells) were carried out in P3 Primary Cell buffer (program CA137) in 16-well cuvettes (Lonza) using an Amaxa 4D-Nucleofector (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: ... using an Amaxa 4D-Nucleofector (Lonza). Synthetic sgRNA (Synthego ...
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bioRxiv - Molecular Biology 2024Quote: ... Cas9 protein (produced in-house) and ssODN (Ultramer DNA oligo, IDT) into iPSC BFP reporter line were carried out in 100uL cuvettes (Lonza) using an Amaxa 4D-Nucleofector (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: ... using an Amaxa 4D-Nucleofector (Lonza), P3 Primary Cell buffer and program CA137 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells in the buffer were then transferred to a 16-well Nucleocuvette™ Strip (Lonza, 4D-Nucleofector™ X Kit) for nucleofection using the pulse code EH-115 ...
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bioRxiv - Molecular Biology 2024Quote: Human cardiac microvascular endothelial cells (HVECs) were purchased from Lonza and were immortalized through transfection of SV40-T-antigen (MiliporeSigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... For HUVEC cells EGM-2 endothelial cell growth medium (Lonza, CC-3162) was used and cells were kept under the same conditions as mentioned before ...
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bioRxiv - Molecular Biology 2024Quote: ... or nucleofected with solution F (Lonza Inc) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... or 5 μL of 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V and transfected into 10 million cells (96 h knockdown) using a NucleofectorTM 2b Device and program T-029 (Lonza). Cells were plated into 6-well plates (5 million cells ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 4 million cells (48 h knockdown ...
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bioRxiv - Molecular Biology 2024Quote: ... and cells were checked regularly for Mycoplasma using the MycoAlert PLUS Mycoplasma Detection kit (Lonza).
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bioRxiv - Molecular Biology 2024Quote: ... grown at 27°C in Insect-Xpress media (Lonza) without shaking was transfected with bacmid plasmid using PEI transfection reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in RDM to OD600 of ≈0.003 and several 1-µl drops of the diluted cell suspension was placed on an agarose pad prepared with RDM and 2% agarose (SeaPlaque GTG Agarose, Lonza). The agarose pad was surrounded with a gene frame (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: The plasmid encoding the bsAb was generously provided by Lonza. The expressed protein is a trimeric IgG bsAb with an LC and HC on one side and a single chain variable chain (HCscFv ...
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bioRxiv - Molecular Biology 2024Quote: ... The HMLE and CRISPR-edited HMLE cells were cultured in MEGMTM mammary epithelial cell growth medium BulletKitTM (Cat. No. CC-3150, Lonza). All cells were cultured at 377#x00B0;C in a 5% CO2 incubator ...