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Citations for Lonza :
1401 - 1450 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and Cas9 variants targeting the c-MYC locus by nucleofector (Lonza, 4D-Nucleofector X). The transfected cells were treated with 1 μg/mL puromycin for 1.5 days after one day post-transfection ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were targeted by nucleofection using the SE Cell Line 4D-Nucleofector™ X Kit S (Lonza #V4XC-1032). Cells were allowed to recover from nucleofection before sib-selection-based subcloning to isolate pure clonal cultures ...
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bioRxiv - Neuroscience 2023Quote: hCMEC/D3 cells (under nine passages) were maintained in EGM2-MV media with VEGF (Lonza, CC4147). Falcon® Permeable Supports for 24-well Plates with 1.0 µm Transparent PET Membranes (Corning ...
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bioRxiv - Physiology 2023Quote: ... and hydrocortisone (EGM SingleQuots Kit, CC-4133, Lonza). HUVECs were used up to passage 4.
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bioRxiv - Physiology 2023Quote: ... were cultured in Endothelial Basal Medium (EBM, CC-3121, Lonza) supplemented with 10% FBS (10270106 ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 2 million cells were nucleofected in a large cuvette per reaction using Lonza’s P3 primary cell 4D-nucleofector kit (V4XP-3024, Lonza, Basel, Switzerland). The cells were allowed to recover from the transfection for 3 days while inducing MNX+ motor neuronal cell fate ...
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bioRxiv - Physiology 2023Quote: Human Umbilical Vein Endothelial Cells (HUVEC, CC-2519, Lonza) were cultured in Endothelial Basal Medium (EBM ...
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bioRxiv - Immunology 2023Quote: ... All the cell culture reagents were purchased from Lonza (USA).
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bioRxiv - Immunology 2023Quote: ... The transfection was carried out by nucleofection using Amaxa® Cell Line Nucleofector Kit V (Program X-001, Lonza, USA). At 24 h after transfection ...
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bioRxiv - Bioengineering 2023Quote: ... Endothelial cells (ECs) were derived from CD34+ stem cells in endothelial cell growth medium (EGM; Lonza cat# CC-3162) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2023Quote: ... All glial preps were tested twice for mycoplasma (Normocin withdrawn) (Lonza, #LT07-318) prior to freezing or neuronal co-culture ...
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bioRxiv - Neuroscience 2023Quote: ... and then passaged by incubation with Versene (Lonza; BE17-711E), then plated in fresh StemFlex onto fresh Geltrex-coated 6-well NUNCTM plates.
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bioRxiv - Immunology 2023Quote: ... BAL was performed by injecting PBS containing 0.01 mM EDTA (Lonza) through a tracheal canula ...
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bioRxiv - Cancer Biology 2023Quote: ... routinely tested for mycoplasma contamination using the MycoAlert Detection Kit (Lonza Group Ltd, LT07-318) and authenticated by STR analysis (CLS ...
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bioRxiv - Cell Biology 2023Quote: ... The cell line was authenticated through short tandem repeat analysis and tested for mycoplasma contamination every 10 passages using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). PDGFR-BiFC and myr-Venus stable cells were cultured in RPMI growth medium [RPMI 1640 (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... Culture media consisted of X-Vivo 15 (Lonza, Cat. # BP04-744Q) supplemented with 2% KnockOut Serum Replacement (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... PiggyBac transposon constructs and RNA encoding transposase were introduced into T cells by electroporation using a Lonza 4D-Nucleovector instrument and the P3 Primary Cell Kit (Lonza, Cat. # V4XP-3032). Electroporated T cells were immediately seeded into 24-well G-Rex plates (Wilson Wolf ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely monitored for mycoplasma and confirmed negative at multiple times during this study using MycoAlert (Lonza). All cells were grown at 37°C with 5% CO2 in a humidified incubator.
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bioRxiv - Immunology 2023Quote: ... fresh cDC2s were cultured in X-VIVO-15 (Lonza) supplemented with 2 % human serum (HS ...
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bioRxiv - Genetics 2023Quote: ... the SG Cell Line 4D-Nucleofector X Kit S (Lonza, #V4XC-3032) was used to transfect THP1 monocytes ...
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bioRxiv - Genetics 2023Quote: ... The SF Cell Line 4D-Nucleofector X Kit S (Lonza, #V4XC-2032) was used to transfect EBV B cells and Daudi cells ...
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bioRxiv - Genetics 2023Quote: Plasmids were transfected using either the 4D Nucleofector (EBV B, Daudi, THP1; Lonza, 4D-Nucleofector Core Unit #AAF-1002B and 4D-Nucleofector X Unit #AAF-1002X) ...
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bioRxiv - Genetics 2023Quote: ... with 20µl of P3 Primary Cell line solution (V4SP-3096, Lonza) and RNP using the cell type program DS-150 ...
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bioRxiv - Genetics 2023Quote: ... Nucleofections were performed using 4D-Nucleofector Core and X unit (AAF-1003B, AAF-1003X, Lonza, Morrristown, NJ, USA). In total ...
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bioRxiv - Immunology 2023Quote: ... Cells lines were regularly tested for presence of mycoplasma using either MycoAlert Mycoplasma Detection Kit (Lonza) or sequencing-based Mycoplasmacheck (Eurofins).
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bioRxiv - Immunology 2023Quote: ... 2×106 or 10×106 cells were resuspended with P3 buffer (Lonza) and mixed with 60 or 300 pmol TRAC RNP in a total volume of 20 or 100 μl ...
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bioRxiv - Immunology 2023Quote: ... 1×10e6 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Electroporation was performed with sgRNA/Cas9 RNP complexes using Alt-R Sp Cas9 Nuclease V3 (IDT) and using the 4D-Nucleofector with P3 Primary Cell 4D Nucleofector X Kit S (Lonza Bioscience).
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bioRxiv - Microbiology 2023Quote: ... BEAS-2B cells (human bronchial epithelial; CRL-9609ATCC) cells were grown in bronchial epithelial cell growth basal media (BEBM; Lonza/CC-3170). LLC-MK-2 cells (rhesus monkey kidney ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were re-suspended in RPMI-1640 medium (Lonza Bioscience) supplemented with 10% FBS (PAA laboratories) ...
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bioRxiv - Molecular Biology 2023Quote: ... MCF-10A human mammary gland cells were used as the non-tumorigenic control and were cultured in MEBM basal medium supplemented with components included in the MEGMTM Mammary Epithelial Cell Growth Medium SingleQuotsTM Kit (Lonza, Hayward, CA). A549 human lung carcinoma cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... and nucleofection was performed as per the manufacturer’s recommendations (Lonza, SG Cell Line 4D-Nucleofector X Kit S). Monoclonal isolation of the knockouts were performed by limiting dilution ...
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bioRxiv - Microbiology 2023Quote: ... and then electroporated with 5 μg plasmid using a Nucleofector 2b Device (Lonza, Germany). The transfected schizonts were immediately intravenously injected into a naïve mouse and pyrimethamine (Pyr ...
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bioRxiv - Microbiology 2023Quote: ... and monitored for mycoplasma contamination using the Mycoplasma Detection kit (Lonza LT07-318). HEK293 cells (ATCC CRL-1573 ...
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bioRxiv - Immunology 2023Quote: ... IPF tissue was injected with 2% low melting agarose (50111, Lonza) and then submerged in ice-cold PBS to allow solidification of agarose ...
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bioRxiv - Biochemistry 2023Quote: ... (Lonza Inc., cat. no. PT5006). Cells at passage two were expanded three times in culture (i.e. ...
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bioRxiv - Cancer Biology 2023Quote: ... Sorted cells were plated in round bottom plates in X-Vivo15 media (Lonza) supplemented with 50 μM 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... The integrity of RNA was verified by resolving in 1% agarose gel containing GelStar nucleic acid gel stain (catalog no. 50535, Lonza, USA) in a Mupid-One gel electrophoresis system (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: ... CAF siRNA transfections were performed by nucleofection using the Amaxa kit R (Lonza) and downstream experiments were performed 5 days post transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell suspension was mixed with a mixture (66 μl per plug) containing 0.83% low-melting-point agarose (SeaPlaque GTG; Lonza), 170 mM sorbitol ...
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bioRxiv - Microbiology 2023Quote: ... 1.2% SeaKem® LE Plus Agarose gel (Lonza, Basel, CH). Recovered amplicon abundance was measured in 384-well plates using a SpectraMax Microplate Reader (Molecular Devices ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 2mM Ultraglutamine I (Lonza), 10 mM HEPES (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... All sorted cells were cultured overnight in X-Vivo15 media (Lonza) supplemented with 50 μM 2-mercaptoethanol ...
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bioRxiv - Immunology 2023Quote: ... using a 4D-Nucleofector (Lonza). After 72h of culture ...
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bioRxiv - Immunology 2023Quote: ... Differentiation was induced at D0 with XVIVO10 medium (Lonza, BE04-380Q) supplemented with 5µM CHIR99 (Miltenyi ...
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bioRxiv - Cancer Biology 2023Quote: ... 200ng of pMaxGFP (Lonza), and ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were tested for the presence of mycoplasma contamination (MycoAlert Mycoplasma Detection Kit, Lonza). For Cerulean labeling ...
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bioRxiv - Neuroscience 2023Quote: ... Electroporation was performed using the Amaxa Cell Line Nucleofector kit V (Lonza Bioscience) with the manufacturer’s settings for N2a cells ...
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bioRxiv - Molecular Biology 2023Quote: ... were cultured in endothelial basal medium EBM (Lonza, Basel, Switzerland; CC-3121) supplemented with SingleQuots (Lonza ...