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Citations for Lonza :
5201 - 5250 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected with the enzyme and with PAK1 or PAK2 sgRNA using nucleofection (4D-Nucleofector™ X Unit, Lonza). Due to low transfection efficiency in leukemia cell lines ...
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bioRxiv - Immunology 2021Quote: ... The infected cells were incubated for 72 h in Dulbecco’s Modified Eagle Medium with L-glutamine (DMEM; Lonza, Walkersville, MD, USA) containing 2% heat-inactivated fetal bovine serum (FBS ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... The cell lines were routinely tested for the presence of mycoplasma contamination using MycoAlert™ Mycoplasma Detection Kit (LT07-318, Lonza), and were also regularly treated with mycoplasma removing agent (093050044 ...
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bioRxiv - Cancer Biology 2021Quote: ... in a total volume of 100μL/ reaction and electroporated using an Amaxa TM Basic Nucleofector TM Kit for Primary Mammalian Epithelial cells (Lonza, # VPI-1005) and the NucleofectorTM 2b Device (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... the antiviral activity of stenoparib on SARS-CoV-2 was assessed in vitro using Vero E6 Cercopithecus aethiops kidney cells (cat # CRL-1586, ATCC, Manassas, VA, USA) maintained in Dulbecco’s Modified Eagle Medium (DMEM; cat #BE12-733F, Lonza, Basel, Switzerland) supplemented with 2 mM L-glutamine (cat # BE17-605E ...
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bioRxiv - Bioengineering 2022Quote: ... that were used to line the vascular channel of the chips were first expanded in microvascular endothelial cell growth medium (EGM™-2MV BulletKit, CC-3202; Lonza) in plastic tissue culture flasks (Falcon 353136 and 355001 ...
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bioRxiv - Developmental Biology 2022Quote: ... and all cell lines are routinely monitored for mycoplasma infection monthly using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat#LT08-118). Stem cells were maintained as previously described (Spence et al. ...
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bioRxiv - Biophysics 2022Quote: ... Cells were negative for mycoplasma contamination and are regularly tested using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, Rockville, MD, USA). Unless stated otherwise ...
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bioRxiv - Genomics 2022Quote: mESC were transfected with sgRNA constructs using the P3 Primary Cell 4D-Nucleofector X Kit (V4XP-3024) and the Amaxa 4D Nucleofector™ system (Lonza). We used the transfection programme CG-104 ...
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bioRxiv - Microbiology 2022Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and to a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC5002); this was repeated to maximize cell collection ...
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bioRxiv - Cell Biology 2019Quote: ... DNA was transiently transfected into HEK SNAP-β2 β-arr-KO cells by electroporation with the Amaxa Nucleofector II device (Lonza). The included program Q-001 is designed for high expression levels in HEK cells and was used according to the manufacture’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 μg of the donor ssDNA and 3 μg of a puromycin resistance plasmid (pCAG-puroR) using the Mouse ES Cell Nucleofector™ Kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... All the primary GBM cell lines were checked periodically for mycoplasma contamination using the MycoAlertTM Mycoplasma Detection Kit (Lonza, Basel, Switzerland). All Patient-derived biological samples were collected according to a protocol approved by Italian Local Ethics Committee (CE IRST IRCCS-AVR ...
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bioRxiv - Biophysics 2019Quote: The HEK293 adherent culture cells were transfected with pcDNA3.4-PCV2d plasmid using Amaxa Nucleofector II instrument by Lonza (program A-23). The transfected cell were plated on 6-well plate ...
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bioRxiv - Cell Biology 2019Quote: To induce expression of fluorescently labeled proteins DCs were transfected according to manufacturer guidelines using nucleofector kit for primary T cells (Amaxa, Lonza Group). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5-2×106 cells were pelleted for each condition and resuspended in 100 μL complete nucleofector solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza) to which 1μg of pCR2.1-mClover-LMNAdonor ...
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bioRxiv - Cell Biology 2021Quote: ... MDCKII cells were transfected by electroporation with plasmids encoding CD4-SIV Env chimeras using an Amaxa Cell Line Nucleofector™ Kit L and Amaxa Nucleofector II with settings L-05 (Lonza). Stable transfectants were selected with 400 μg/ml G418 Sulphate (Calbiochem) ...
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bioRxiv - Cell Biology 2021Quote: ... All cell lines used in this study were monitored for mycoplasma infection using the MycoAlert Mycoplasma Detection Kit (Lonza TL07-218).
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bioRxiv - Developmental Biology 2020Quote: ... or a PMO Control at 100 µM in 100 µL solution from the P3 Primary Cell 4D-Nucleofector® X Kit L (V4XP-3024, Lonza) using the CB150 program on the 4D-Nucleofector™ System (Lonza) ...
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bioRxiv - Neuroscience 2020Quote: ... Cell media was collected and the presence of adenylate kinase was measured with the ToxiLightTM cytotoxicity assay kit (Lonza, Basel, Switzerland) to check the integrity of the cell membrane ...
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bioRxiv - Neuroscience 2019Quote: ... or a non-silencing plasmid immediately before plating (Amaxa™ P3 Primary Cell 4D-Nucleofector X Kit L; Lonza, CU133 program). The generation and knockdown efficiencies of shRNA plasmids were described previously (Guner et al. ...
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bioRxiv - Genetics 2021Quote: ... complex and mix of 1:1 ratio WT/mutant donor single-stranded oligodeoxynucleotides carrying PAM mutation by electroporation using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. sgRNA and donor single-stranded oligodeoxynucleotide sequences can be found in supplementary table 5 ...
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bioRxiv - Cancer Biology 2021Quote: All cell lines used in this study were confirmed mycoplasma-free by an in-house testing regimen (MycoAlert, Lonza; LT07-703). MDA-MB-231 cells from the American Type Culture Collection (ATCC ...
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bioRxiv - Immunology 2021Quote: ... The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza, Walkersville, MD, USA), in the absence or presence of 100 U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... were washed in PBS/0.5%BSA and resuspended in 100 µl Nucleofector solution (Amaxa™ P3 Primary Cell 4D Nucleofector TM X Kit L, Lonza) with 4-6 µl of the appropriate siRNA (20-40 µM ...
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bioRxiv - Genetics 2021Quote: Human K562 cells were nucleofected with already complexed RNP using the SF Cell Line 4D Nucleofector X Kit for Amaxa 4D device (Lonza, Basel) with program FF-120 ...
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bioRxiv - Cell Biology 2020Quote: U2OS FlpIN TRex cells with stable dox-inducible expression of MLS-EGFP-mCherry (referred to as IMLS cells) 11 were grown and maintained in a complete medium of Dulbecco’s Modified Eagle Medium (DMEM – Lonza 12-741F) supplemented with 10% v/v foetal bovine serum (FBS – Sigma Aldrich #F7524 ...
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bioRxiv - Genomics 2021Quote: The MPRA libraries were electroporated in 4 to 6 million cells each using the Nucleofector 2b or 4D (Lonza, Basel, Switzerland) with 6 µg of plasmid DNA and program T-030 or Y-001 and DS-132 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-Fc stable clone was obtained by electroporation with 2×106 cells and 5 μg endotoxin-free plasmids using Amaxa kit V and program U24 with Amaxa Nucleofector 2B (Lonza, Switzerland). Electroporated cells were subsequently plated in 96-wells at 500 cells/well in Plating Medium containing 80% EX CELL® CHO Cloning Medium (Cat.no C6366 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated rat commissural neurons were electroporated with the Amaxa 96-well Shuttle using the P3 Primary Cell 96-well Nucleofector Kit (Lonza, Switzerland). For each electroporation in one well (20 µl ...
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bioRxiv - Biochemistry 2022Quote: ... HCT116 human colorectal carcinoma and CRISPR/Cas9-mediated METTL11A knockout (KO) HCT116 cell lines were cultured in McCoy’s 5A Modified Medium (Lonza, Walkersfield, MD) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Cancer Biology 2022Quote: ... and NCI-H1299 cell lines were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Lonza; Cat. No. 12–115F/12) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: Whole peripheral blood samples from either healthy donors or AML patients were processed to obtain mononuclear cells (PBMCs) through density gradient centrifugation using Lympholyte™ (cat#DVCL5020-10 from Lonza). CD3+ T lymphocytes were isolated via magnetic separation with a Pan T cell Isolation Kit (cat#130-096-535 from Miltenyi Biotech ...
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were found to be free of mycoplasma using the MycoAlert® PLUS Mycoplasma Detection Kit (Lonza #LT07-703).
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bioRxiv - Cell Biology 2022Quote: ... Dissociated muscles were passed through a 70µm filter and red blood cells were lysed in ACK lysis buffer (Lonza #10 548E). Cell suspensions were then incubated with anti CD45 ...
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bioRxiv - Genomics 2022Quote: ... We used 1.0 × 106 HCT116 or U2OS cells to transfect the base editor plasmids (2600 ng) using SE solution and 4D-nucleofector (Lonza, Switzerland) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained by twice-weekly passage and checked for mycoplasma contamination regularly using a MycoAlert mycoplasma detection kit (Lonza, UK). IAV strain A/California/04/2009 (H1N1 ...
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bioRxiv - Physiology 2022Quote: ... 1 million cells were washed with PBS and electroporated with 5-10 μg of appropriate plasmid using Amaxa cell nucleofector kit T (Lonza laboratories). Cells were allowed to recover for 48 h ...
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bioRxiv - Immunology 2022Quote: ... blood was isolated using the retroorbital route and red blood cells lysed twice using Lonza ACK Lysing buffer (Lonza, Basel, Switzerland), then subjected to staining as described above ...
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bioRxiv - Physiology 2022Quote: ... 5 million cells were washed with phosphate buffered saline (PBS) and electroporated with 4-6 μg of appropriate plasmid construct using an Amaxa cell nucleofector (Lonza laboratories) and nucleofection reagent (362.88 mM ATP-disodium salt ...
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bioRxiv - Neuroscience 2023Quote: ... Nucleofection was performed using the 4-D nucleofector system (AMAXA) and the P3 Primary Cell 4D-Nucleofector Kit (Lonza, V4XP-3024) following manufactures’ instructions (program CB-150) ...
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bioRxiv - Neuroscience 2022Quote: The cell toxicity was determined by measuring the relative amount of adenylate kinase released from cells into the medium (ToxiLightTM Non-Destructive Cytotoxicity BioAssay Kit, LONZA, Swittzerland). Medium from cell culture was mixed with adenylate kinase substrate adenosine diphosphate ...
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bioRxiv - Molecular Biology 2022Quote: ... collected by centrifugation at 500xg for 5 min and resuspended in SF solution (SF cell line 4D-Nucleofector™ X Kit, Lonza). After addition of the RNP ...
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bioRxiv - Microbiology 2022Quote: ... cells were seeded at 1-3×104 cells/cm2 whilst in the log phase of replication with Dulbecco’s minimal essential medium (DMEM) (BE12-604F, Lonza Group AG) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... 10e6 T cells per screening condition were transferred to one T75 flask in 20 ml of X-VIVO 15 (Lonza Bioscience) supplemented with 5% FCS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Dulbecco’s Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI) used to culture cells were obtained from Lonza (NSW, Australia). GlutaMax ...
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bioRxiv - Immunology 2024Quote: ... Lysis of red blood cells was performed using 300 μl of Ammonium-Chloride-Potassium (ACK) buffer for 1.5 minutes (Lonza, Basel Switzerland). Blood was collected in EDTA-coated tubes (MiniCollect ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 2 million cells were nucleofected in a large cuvette per reaction using Lonza’s P3 primary cell 4D-nucleofector kit (V4XP-3024, Lonza, Basel, Switzerland). The cells were allowed to recover from the transfection for 3 days while inducing MNX+ motor neuronal cell fate ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines were confirmed to be mycoplasma negative every 3 weeks (MycoAlert™ Mycoplasma Detection Kit, Lonza, Bend, OR, USA). Fifteen micrograms of total whole cell lysates (WCL ...
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bioRxiv - Cancer Biology 2024Quote: MCF-10A cell line was obtained from American Type Culture Collection (ATCC) and cultured in mammary epithelial cell growth basal medium (MEBM) with single quots supplements and growth factors (Lonza, #CC-4136). Alternatively cells were cultured in Human Plasma-like Medium (HPLM ...