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Citations for Lonza :
5151 - 5200 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... nucleofected in a 100 µl reaction using the Lonza 4D-Nucleofector System with P3 Primary Cell 4D-Nucleofector X Kit (Lonza) and program CA137 ...
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bioRxiv - Microbiology 2024Quote: ... Human airway tracheobronchial epithelial cells (NHBE) isolated from airway specimens from four donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Biophysics 2024Quote: ... 0.5 μg of F-tractin-mScarlet plasmid and 0.75 ug of Zyxin-mNeonGreen plasmid were mixed with solution SE from SE Cell Line 4D-Nucleofector S Kits (Lonza). 4×105 cells were mixed with the plasmid-SE solution and nucleofected with program CM-137 using a 4D-Nucleofector (Lonza).
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were authenticated by Multiplex Cell Line Authentication (MCA) and were tested for mycoplasma by MycoAlert Mycoplasma Detection Kit (Lonza). HEK293T cells were transfected with the plasmids using 1mg/ml polyethylenimine ...
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bioRxiv - Biophysics 2024Quote: ... 4×105 cells were mixed with the plasmid-SE solution and nucleofected with program CM-137 using a 4D-Nucleofector (Lonza).
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bioRxiv - Bioengineering 2024Quote: ... The transfection reaction was transferred into P3 Primary cell cuvette and electroporated with EO-115 program on a Lonza 4D Nucleofector (Lonza). The cells were rested in the cuvette without disturbing them for 15 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... All primary cells were used at passages below 10 and were regularly tested for mycoplasma using the MycoAlert Mycoplasma Detection kit (Lonza). For the onset of transgenic expression cells were treated with 200 ng/mL DOX for 48 hours before being subjected to downstream assays unless otherwise specified.
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bioRxiv - Cancer Biology 2024Quote: ... CB HSCs and BM HSPCs were cultured for 48h and AML cells for 24h and then CRISPR/Cas9 RNP electroporation was performed using the 4D-Nucleofector (Lonza), chemically synthesized gRNAs (IDT ...
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bioRxiv - Microbiology 2024Quote: ... aspirate supernatant and cover the cell monolayer with 3ml per well of 1.5% Sekam ME Agarose (Lonza; Cat. No. 50011), 2X EMEM (quality Biological ...
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bioRxiv - Immunology 2024Quote: ... 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza). For the CLEM experiments the cells were transfected with TSP-1-GFPSpark and TSP-4-mCherry ...
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bioRxiv - Molecular Biology 2024Quote: ... and pCMV-Pbase (0.2µg) (kindly provided by L. Tiberi) using P1 Primary Cell 4D-NucleofectorTM X Kit L (Lonza; V4XP-1024) Amaxa Nucleofector (program FF104 ...
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bioRxiv - Molecular Biology 2024Quote: ... Schizonts were transfected by electroporation using the Lonza 4D Nucleofector System according to the pulse program FI-115 with the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). After transfection ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl of isolated schizonts was mixed with 7 μl of DNA and 18 μl of P3 Primary Cell 4D-Nucleofector solution from Lonza. This mixture was then added to a well within a 4D-Nucleofector 16-well strip from Lonza and electroporated using the FI-115 program on the Amaxa Nucleofector 4D ...
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bioRxiv - Molecular Biology 2024Quote: ... 2×105 cells were electroporated with 250 ng of gRNA plasmids and 750 ng of CBE using the SF Cell Line Nucleofector X Kit (Lonza) via the 4D-Nucleofector system ...
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bioRxiv - Immunology 2024Quote: ... They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza) using the V-001 program (Amaxa Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... Sorted cells expressing medium levels of fluorescence were expanded and frozen then representative lots of each cell line were thawed and tested for mycoplasma contamination using the MycoAlert assay (Lonza), and fungi and aerobic bacteria sterility by inoculating conditioned medium into trypticase soya broth (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... with a density of 1.5 x106 using a 1:70 inoculation from the V1 stock for 92 hours at 21°C in Insect Xpress Protein-free Insect Cell Medium (Lonza) supplemented with GlutaMAX (GIBCO ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were routinely tested for Mycoplasma using a MycoAlert™ PLUS Mycoplasma Detection Kit (Cat# LT07-703, Lonza).
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bioRxiv - Cell Biology 2024Quote: ... The cell lines used in this study were routinely tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (#LT07-318, Lonza), and mycoplasma-negative cells were used ...
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bioRxiv - Immunology 2024Quote: ... 3 x 106 freshly isolated monocytes or BMDMs were resuspended in 20 µl of P3 primary cell nucleofection buffer (Lonza). Cells were then added to the Cas9-RNP complexes ...
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bioRxiv - Genomics 2024Quote: A Cas9/sgRNA RNP complex and linear dsDNA donor were transfected into HCT116 cells using the Amaxa Nucleofector II device (Lonza) set for program D-032 with the Cell Line Nucleofector Kit V ...
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bioRxiv - Synthetic Biology 2024Quote: ... Nucleofector program 96-FF-120 was used to electroporate K562 cells with the pDNA mix on the Amaxa Nucleofector 96-well Shuttle System (Lonza). After electroporation ...
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bioRxiv - Synthetic Biology 2024Quote: ... Nucleofector program 96-FF-120 was used to electroporate K562 cells with the pDNA mix on the Amaxa Nucleofector 96-well Shuttle System (Lonza). After electroporation ...
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bioRxiv - Cancer Biology 2024Quote: ... were annealed to Cas9 protein (IDT) and the ribonucleoprotein complex was transfected into SKOV-3 cells using a 4D-Nucleofector (Lonza). Bulk transfected cells were checked by flow cytometry for successful knock-down ...
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bioRxiv - Genomics 2019Quote: ... The DNA fragments of 150–200 bp length were gel-extracted on 3% NuSieve 3:1 Agarose (Lonza). Both samples were sequenced by GATC Biotech AG (Konstanz ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were then diluted 1:5 in endotoxin free water in pyrogen-free glass tubes (Lonza N207) and tested for endotoxin in duplicate using a Kinetic-QCL™ LAL Assay (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... at 0.1-1 μg/mL or as a control a GFP plasmid (PmaxGFP, Lonza, Cat. No. D-00059) using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... 5×104−1×105 fresh isolated DRG neurons were transfected with 0.5 μg Mito-GFP for Nucleofector (Lonza) SCN Basic Neuro Program 6 with Amaxa Basic Neuron SCN nucleofector Kit ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% penicillin-streptomycin (P/S: 100 U/ml penicillin and 100 U/ml streptomycin, Lonza, Basel, Switzerland) in 15 cm cell culture plates (Nunc ...
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bioRxiv - Biophysics 2019Quote: ... 1.5 μg Cas9 vectors, and 1 μL ssODN (4μg, IDT) and then electroporated with a 4D-Nucleofector (Lonza) using the CA-137 program ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were electroporated with 0.4 μg pmaxGFP plasmid and different concentrations of SGM and SAM (0.01, 0.1, 1 mM) using 4D-Nucleofector X Unit from Lonza. EH-100 program was used for HepG2 by following the manufactures protocol ...
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bioRxiv - Microbiology 2021Quote: ... the inoculum was removed and covered with agar-overlay media including 1% (w/v) low-melting agarose (Lonza) and 2% FBS in DMEM ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA substrates were separated by gel electrophoresis using 1% SeaKem LE Agarose gel (Lonza Group AG, Basel, Switzerland). The bands were excised and purified using QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 100000 cells were transfected with BAG-1 sgRNA (6μM; Synthego) and Cas9 2NLS (0.67μM; Synthego) using the 4D Nucleofector System (Lonza Bioscience). After 48 hours ...
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bioRxiv - Plant Biology 2023Quote: ... and PCR products were separated by agarose gel electrophoresis in an 1% w/v agarose gel (Lonza, 50004) using 100V during 30 min ...
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bioRxiv - Immunology 2023Quote: ... 1999) cultured in I10 media (Iscove’s modified Dulbecco’s Medium, 10% FBS, 1:1000 MycoZap; Thermo Fisher and Lonza) supplemented with 100 ng/mL IL-21 (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... program A-30 with 10 µg of plasmid DNA and 1 µg of eGFP control plasmid (Amaxa, Lonza) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... Mycoplasma detection was routinely performed to ensure cells were not infected with mycoplasma by using MycoAlert Detection kit (Lonza, LT07-218). Cells were maintained at 37°C in a 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to the well of a 16-well Nucleocuvette™ strip (SF Cell Line 4D-NucleofectorTM X Kit S, Lonza). Cells were electroporated on a 4D-NucleofectorTM X Unit (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... Feng and Coulombe 2015b) were transfected into Krt14-/- skin keratinocytes using P1 Primary Cell 4D-Nucleofector™ X Kit (V4XP-1024, Lonza). After nucleofection ...
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bioRxiv - Cell Biology 2019Quote: ... was transfected into skin keratinocytes in primary culture using the P1 Primary Cell 4D-Nucleofector™ X Kit (V4XP-1024, Lonza).
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bioRxiv - Cell Biology 2020Quote: ... The cells were tested for mycoplasma on receipt of the cell line and quarterly thereafter using the MycoAlert Mycoplasma Detection Kit according to the manufacturer’s instructions (Lonza, Basel, Switzerland).
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bioRxiv - Developmental Biology 2020Quote: ... and nucleofected with BioID constructs using an Amaxa Nucleofector II and the Mouse Neural Stem Cell Nucleofector Kit (Lonza; VPG-1004) according the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The Cas9-sgRNA mixture was added to the cells to a final volume of 20 μL and transferred to 16-well Nucleocuvette™ strips (Lonza). Pulse was applied utilizing the CM-130 program for HEK293 cells and EA-104 for RPE1 cells ...
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bioRxiv - Cell Biology 2020Quote: ... 5 × 103 cells were plated in low-attachment 96-well plates containing 100 μL MEGM media (without BPE) (Lonza, CC-3150), 20 ng/mL bFGF (Sigma Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... Cell lines were authenticated by STR profiling and mycoplasma testing was performed using the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza). U2OS cells were grown in DMEM supplemented with 10% tetracycline-free foetal bovine serum (Clontech) ...
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bioRxiv - Neuroscience 2020Quote: ... All cells were routinely tested for mycoplasma contamination with MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, ME, USA, Cat N° LT07-318). Chemicals and cell culture reagents were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cell suspension with trypsin-EDTA (Pan-Biotech, Germany) for 3 min followed by trypsin neutralizing solution (Lonza, Germany). Single cell suspensions of secondary mammospheres were obtained as described for day 7-first generation mammospheres.
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bioRxiv - Cancer Biology 2020Quote: ... while MCF-10A cells were cultured as per ATCC recommendations in MEGM bullet kit growth media (CC-3150; Lonza, Walkersville, MD) without gentamycin-amphotericin B mix but with additional 100ng/ml cholera toxin (C8052 ...
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bioRxiv - Cancer Biology 2019Quote: 2.106 cells were electroporated with double-stranded siRNA to a final concentration of 2µM using an electroporation device (Lonza 4D Nucleofector) according to the manufacturer’s specifications ...