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Citations for Lonza :
5251 - 5300 of 5517 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Electroporation was performed with sgRNA/Cas9 RNP complexes using Alt-R Sp Cas9 Nuclease V3 (IDT) and using the 4D-Nucleofector with P3 Primary Cell 4D Nucleofector X Kit S (Lonza Bioscience).
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bioRxiv - Immunology 2024Quote: ... 5×104 APC were seeded together with 1×105 CD4+ T cells in the presence of relevant peptides at 2.5 ng/ul diluted in X-VIVO 15 medium (Lonza, Basel, Switzerland) supplemented with 4 % human AB plasma (Innovative Research ...
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bioRxiv - Biophysics 2023Quote: ... 1.5mL for a T75 flask for 5 minutes at 37° C and resuspended in 80 μL of SF cell line solution (Lonza; Basel) per flask ...
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bioRxiv - Genomics 2024Quote: ... macrophages were scraped and counted to achieve 0.5×106 cells were prepared in 20ul P3 buffer for electroporation (LONZA - 4D Nucleofactor). To each 20ul macrophage suspension in P3 buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were cultured at passage 3 and seeded in triplicate in hMSC Growth Medium (MSCGM™ Mesenchymal Stem Cell Growth Medium, Lonza) with 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... The day of the electroporation cells were collected and washed twice with phosphate buffered saline (PBS) and resuspended in nucleofection buffer (SF/SE Cell Line 96-well Nucleofector™ Kit; Lonza) containing 2 μg of PEn mRNA and 100 pmol of synthetic springRNA or spaceRNA ...
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bioRxiv - Cell Biology 2023Quote: ... These guide constructs were then electroporated into mESCs using the Lonza Nucleofector 2b Device and Cell Nucleofector Kit (Lonza #VAPH-1001). mESCs were cultured in feeder-free 2i media [DMEM/F-12 (Gibco #11320033 ...
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bioRxiv - Immunology 2022Quote: T cells were transfected with control mRNA or ME1 mRNA (220 μg/ml) using the P3 nucleofection kit (Lonza V4XP-3024) and program FI-115 on the 4D Nucleofector (Lonza) ...
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bioRxiv - Genomics 2023Quote: ... and 120 pmol of in vitro synthesized gRNA were electroporated into 250,000 MCF7 cells with the P3 primary nucleofection solution (Lonza, V4XP-3024), using the DN-100 Lonza 4D-Nucleofector program ...
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bioRxiv - Genetics 2023Quote: All iPSC lines were passaged as clumps or single cells upon reaching 80-85% confluency using Versene (0.02% EDTA solution, Lonza #17-711E) or StemPro Accutase (Gibco #A1110501) ...
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bioRxiv - Microbiology 2023Quote: ... Pooled sgRNA and healing template were added to the cell suspension and nucleofected using a Lonza Nucleofector 2b (Lonza, Basel, Switzerland) using program X-014 ...
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bioRxiv - Cell Biology 2023Quote: ... and progenitors isolated from day 14 medium-sized CFU-M were suspended in Endothelial Cell Growth Medium (Lonza, Cat# CC-4542) and plated on growth factor-reduced MatrigelTM (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... TG trigeminal ganglion neurons were transfected with 3 µg of NaV1.7-PEPCy3 plasmid and 0.75 µg green fluorescent protein (GFP) using the rat neuron 4D-Nucleofector solution (P3 Primary Cell Solution, program DR 114; Lonza Biosciences). Neurons were plated on round bottom glass dishes (cat ...
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bioRxiv - Cancer Biology 2022Quote: ... the activated CD8+ T cells were transduced with mcDNA-CD19-CAR by using preset program EO115 of 4D-Nucleofector™ system (Lonza). Electroporation was performed according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with pcDNA::3xflag-legC4 or pcDNA::3xflag vector (Table S2) using a Nucleofector 2b electroporator (Lonza, Basel, Switzerland) and cultured over 14 days with Zeocin selection (200 -1000 µg mL- 1) ...
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bioRxiv - Immunology 2023Quote: ... PBECs were scraped carefully from the luminal surface of the bronchus without disturbing the mucosal surface integrity and cultured in bronchial epithelial cell growth medium (BEBM; Lonza, Belgium) supplemented with antibiotics ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured until ∼80% confluence before being passaged and seeded at 10,000 cells/cm2 in EGM-2MV (LONZA, Cat# CC-3202) in 6-well tissue culture plates and were allowed to adhere for 5 hours before induction of serum starvation in EBM-2 (LONZA ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were obtained from ATCC and were tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland). HCT116 (RRID:CVCL_0291 ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection in the appropriate cell lines was achieved by nucleofection using program X-014 of the AMAXA Nucleofector® apparatus (Lonza) as previously described (Burkard et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Complete culture media for each cell line was as follow: FaDu and Detroit562 (Eagle’s minimal essential medium (EMEM) (Lonza, #12-125Q) with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells from six independent donors (CC-2517/0000242397, C2517A/0000088928, C2517A/0000439577, C2517A/0000306191, CC-2517/0000198228, C2517A/0000699238: Lonza, TX) were used for transcriptomic analyses and validation ...
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bioRxiv - Genetics 2023Quote: ... The RNP complex together with 7 µg of donor DNA was nucleofected into 3 × 107 ISE6 cells using EN150 and buffer SF via the 4D-Nucleofector system (Lonza Bioscience) (Figure S1) ...
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bioRxiv - Cancer Biology 2023Quote: ... We periodically tested all the cell lines for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, cat. no. LT07-118). Culturing conditions were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines (and their derivatives) were confirmed to be mycoplasma-free using the MycoAlert mycoplasma detection kit (Lonza LT07- 218) at the beginning and upon completion of all experiments.
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... MDA-MB-231 and PC3 cells with FBXO22 CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Two sgRNAs targeting FBXO22 gene (FBXO22 sgRNA#1 ...
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bioRxiv - Bioengineering 2024Quote: ... All stem cell and organoid lines were routinely monitored for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza Cat#LT07-318).
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bioRxiv - Genomics 2024Quote: ... Cell lines in culture were screened biweekly for mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, 801 #LT07-703).
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bioRxiv - Biochemistry 2024Quote: ... 5.0 × 106 OSCs were mixed with 200 pmol of siRNAs in 20 μL of Solution SF of the Cell Line Nucleofector Kit SF (Lonza Bioscience) and electroporation was performed using a Nucleofector 96-well Shuttle device (Lonza Bioscience) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Then the supernatant was removed and cells were resuspended in 100 µl room-temperature Nucleofector® Kit V (Lonza, VCA-1003) solution ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 µL of cell suspension (200,000 cells) was mixed with the RNP-containing solution and electroporated using the 4D-Nucleofector X Unit (Lonza, Basel, Switzerland) with the CM138 nucleofection program ...
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bioRxiv - Cell Biology 2020Quote: ... mouse skeletal myoblasts were grown at 37°C with a 5% CO2 humidified atmosphere in Dulbecco’s Modified Eagle’s Medium with 4.5 g.L−1 Glucose (DMEM; Lonza Bioscience, Bâle, Zwitzerland), supplemented with 10% fetal Bovin Serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were washed 3-4 times over 1 hour in PBS (calcium- and magnesium-free; Lonza BioWhittaker #17-517Q) containing 0.1% Triton X-100 (PBT ...
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bioRxiv - Cell Biology 2021Quote: ... dissected samples were pulled onto a coverslip containing an agarose pad made of 1% low-melt agarose (Lonza #50100) dissolved in Tyrode’s buffer ...
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bioRxiv - Cancer Biology 2019Quote: HEK293T was maintained in DMEM (Dulbecco’s modified Eagle’s high glucose medium) supplemented with 10% (v/v) FBS (foetal bovine serum) and 1% antibiotic/antimycotic (Lonza) in a humidified incubator with 10% CO2.
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bioRxiv - Biochemistry 2020Quote: HEK293T cells were maintained in DMEM (Dulbecco’s modified Eagle’s high glucose medium) supplemented with 10% (v/v) FBS (foetal bovine serum) and 1% antibiotic/antimycotic (Lonza) in a humidified incubator with 10% CO2.
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bioRxiv - Bioengineering 2019Quote: K562 cells were transfected with two gRNAs targeting exon 1 and 3 of NGLY1 and Cas9 plasmid (lentiCas9-Blast) by Nucleofection according to the manufacturer’s protocol (Nucleofector, Lonza). LentiGuide-Puro (Addgene plasmid #52963 ...
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bioRxiv - Neuroscience 2021Quote: ... the reaction was stopped by adding deactivated fetal calf serum (FCS; HyClone) and basic medium (1 % Penicillin/ Streptomycin (Lonza), 10 % FCS ...
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bioRxiv - Developmental Biology 2021Quote: ... All larvae were anaesthetised using 0.02% tricaine and then embedded on their side in 1% low melting agarose (Lonza) on a glass bottom dish (MalTEK Corporation ...
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bioRxiv - Developmental Biology 2020Quote: ... Wells were mounted by pouring 1 ml of 2.5% melted agarose (50080, Lonza Rockland, ME in Danieau buffer (DBx1) into the μ-dish with the mold inside ...
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bioRxiv - Cell Biology 2021Quote: ... The harvested EV pellet was resuspended in 1 mL RNase-free Phosphate buffered saline (RNase-free PBS, Lonza, Australia) and washed using ultracentrifugation at 100,000 × g for 60 min at 4°C ...
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Receptor tyrosine kinases regulate signal transduction through a liquid–liquid phase separated statebioRxiv - Cell Biology 2019Quote: ... A431 and MCF7 cells were maintained in DMEM (Dulbecco’s modified Eagle’s high glucose medium) supplemented with 10% (v/v) FBS (foetal bovine serum) and 1% antibiotic/antimycotic (Lonza) in a humidified incubator with 10% CO2 ...
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UC2 – A Versatile and Customizable low-cost 3D-printed Optical Open-Standard for microscopic imagingbioRxiv - Bioengineering 2020Quote: ... PBMCs were seeded at a density of 1 x 106cells/cm2 in X-VIVO 15 medium (Lonza, Cologne, Germany) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... anesthetized by incubation in egg water containing 0.003 % Tricaine and embedded laterally in 1 % low melting agarose (Lonza 50081) containing 0.16 mg/mL Tricaine in glass-bottom dishes (MatTek ...
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bioRxiv - Biophysics 2023Quote: ... tsa-201 cells were transfected via Lipofectamine 2000 24-48 hours prior to experiments with 1 μg pCMV6-SCN9A (gift from Dr. Christoph Lossin) and 0.5 μg pMaxGFP (Lonza) for identification of transfected cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Smiths Medical) were connected to two 1-mL gas-tight syringes (SGE or Hamilton) and filled with PBS (Lonza). The tubing was manually filled with PBS ...
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bioRxiv - Genetics 2023Quote: ... Fixed larvae were transferred to 0.17 mm imaging plates (FlouroDish) and embedded in 1% low melting agarose gel (SeaPlaque Agarose, Lonza) and imaged using a super resolution microscope-Leica STED 3X at 63x water lens.
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bioRxiv - Developmental Biology 2023Quote: ... using Vectashield® mounting medium (Vectorlabs, Newark, CA, USA) and embedded in 1% low melting agarose (Lonza, Basel, Switzerland). Images were obtained using a SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Cell Biology 2023Quote: ... 1xPenStrep (cat#XC-A4122, Biosera, Nuaille, France) and 1% nonessential amino acid (NEAA, cat#BE13-114E, Lonza, Basel, Switzerland) and maintained at 37 °C under 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... GV samples at final OD500 = 1.8 were loaded into the phantoms made of 1% agarose (w/v, Lonza, #50070) in those buffers and incubated at 37°C for >10 minutes before ultrasound imaging.
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence microscopy to visualize lipid droplets was performed on mature adipocytes using AdipoRed (1:250 dilution, Lonza #PT-7009) and Hoechst 33342 (1 μg/mL ...