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Citations for Worthington Biochemical :
1 - 50 of 496 citations for Rat Heat Shock 60 kDa Protein 1 Chaperonin HSP60 HSPD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 60 units of DNase I (Worthington). The cell suspension was centrifuged at 160 g for 5 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... 60% papain (Worthington, Lakewood, NJ, United States), 20% DNase (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: Primary rat cortical neurons were prepared from E18-19 embryos using a papain dissociation kit (Worthington Biochemical) following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... 3-4 pooled organoids at 3-4 months of age or three single organoids at 2-month age from both haploinsufficient and corrected lines were transferred to a 60 mm dish containing a papain and DNase solution from the Papain Dissociation Kit (Worthington, #LK003150). Organoids were minced into small bits with razors and incubated for 30 min at 37°C on an orbital shaker ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... Cells were crosslinked with 1% formaldehyde and then 2.5 x 106 cells were treated with 60 U of MNase (Worthington, LS004797) and Exonuclease III (E ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... for 60-75 min and treated with inactivating solution (Worthington) for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... and 60 U/mL of Collagenase Type I from Worthington. The perfused liver was carefully excised out from the abdomen and transferred into a petri dish containing 1X HBSS ...
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bioRxiv - Neuroscience 2023Quote: ... for 60-75 min and treated with inactivating solution (Worthington) for 10 min ...
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bioRxiv - Pathology 2024Quote: ... and 60 U/mL DNase I (Worthington Biochemical Corporation: LS006333) in RPMI-1640 ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. ...Cat# LS006333, 10 ku, $176.00 Ask -
bioRxiv - Neuroscience 2021Quote: Primary hippocampal neurons were isolated from either E18 rat embryos or P0 mouse pups using a Papain Dissociation Kit according to the manufacturer’s protocol (Worthington Biochemistry). Cells were plated on poly-D-lysine (Sigma Aldrich ...
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bioRxiv - Bioengineering 2020Quote: P1 neonatal rat cardiomyocytes were isolated from Sprague Dawley rat pups with a Neonatal Cardiomyocyte Isolation System (Worthington Enzyme). Following manufacturer instructions collecting cardiac cell suspension from neonatal myocardial rat tissue ...
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bioRxiv - Physiology 2019Quote: ... Heart tissue (infarct and surrounding border) was digested in Collagenase II and DNase 1 (600 U/ml Collagenase II; CLS-2 Worthington, 60 U/ml DNAse 1; Ambion, Warrington) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Cell Biology 2019Quote: Ventricular cardiomyocytes isolated from rats or mice at postnatal day 2 or day 1 respectively using the Neonatal Cardiomyocyte isolation System (Worthington) were cultured as previously reported.22 To induce the deletion of Psmc1 ...
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bioRxiv - Molecular Biology 2019Quote: Adult rat ventricular fibroblasts (ARVFs) were isolated from female Sprague Dawley rats using enzymatic digestion (1 mg/ml collagenase type II; Worthington Biochemical Corporation) followed by gentle centrifugation for collection of cells ...
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bioRxiv - Neuroscience 2024Quote: ... pooled hippocampi of E18 rat embryos were dissociated enzymatically by trypsin treatment (0.1%; Worthington) with DNase (50 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were rinsed in cold RNase-free PBS and transferred to freshly made dissociation buffer (300 μL of 100 U/mL heat-activated papain (Worthington Biochemical Corporation #LK003178) added with 6 μL of 2.5 mg/mL liberase™ (Sigma-Aldrich #5401119001)) ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Biophysics 2024Quote: ... The morselized ovary was washed in Ca2+-free buffer to remove yolk from lysed oocytes and then digested at room temperature with gentle shaking for 60-70 minutes in 0.5 mg/ml collagenase type 2 (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2021Quote: ... cortices were dissected from E19 rat pups or E17.5 mice pups and dissociated with papain (Worthington Biochemical). Neurons were plated at 75,000 cells per well on 12-wells plates ...
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bioRxiv - Bioengineering 2023Quote: ... Hippocampal neurons were extracted from neonatal rat (Sprague Dawley, 001) pups (P1) and dissociated with Papain (Worthington Biochemical). The cells were then seeded on glass slides (5 mm diameter ...
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bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
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bioRxiv - Pathology 2021Quote: ... Retained proteins were digested with trypsin (Worthington), at a ratio of 10:1 protein to trypsin ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with trypsin (Worthington-Biochem) in 50 mM TEAB pH 8.0 at an enzyme-to-protein ratio of 1:10 (w/w ...
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bioRxiv - Neuroscience 2021Quote: ... and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington, Cat #:LK003150). The tissue was mechanically triturated and suspended cells were isolated and resuspended in EBSS with albumin-ovomucoid inhibitor solution ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... the cortical tissue was incubated with constant shaking at 37°C for 40 minutes in 1 ml of papain (20 U/ml) dissolved in the isolating solution supplemented with 60 µl of DNase (both from Worthington, Lakewood, NJ, USA). During the enzymatic dissociation ...
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bioRxiv - Neuroscience 2019Quote: ... following genotyping or embryonic day 18 embryos (rat) and dissociated enzymatically for 20 min at 37 °C in HBSS supplemented with 0.25% (w/v) trypsin (Worthington Cat# LS003707), followed by mechanical dissociation via trituration with fire-polished glass Pasteur pipettes ...
Product cited:
Supplied as a chromatographically purified, diafiltered and lyophilized powder.Cat# LS003707, 1 gm, $325.00 Ask -
bioRxiv - Plant Biology 2023Quote: ... Proteins were digested by adding bovine trypsin (Worthington-LS003750) and endoproteinase Lys-C (NEB-P8109S ...
Product cited:
Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl...Cat# LS003750, 100 mg, $170.00 Ask -
bioRxiv - Genomics 2022Quote: Primary cultures of neonatal rat ventricular cardiomyocytes (CM) were isolated as previously described using the neonatal cardiomyocyte isolation system (Worthington Biochemical Corporation) from 1-3 day old Fischer rat pups (Golan-Lagziel et al. ...
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bioRxiv - Neuroscience 2020Quote: Sprague-Dawley rat frontal cortexes were isolated from postnatal day 0 pups and enzymatically treated with papain (50 u/mL; Worthington Biochemical Corp., Lakewood, NJ, USA). The cells were then triturated in glass pipettes of decreasing size to create a uniform cell suspension and plated at appropriate confluence onto cover slips coated with laminin (2 μg/mL ...
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bioRxiv - Developmental Biology 2020Quote: Two organoids per experimental condition were cut into 1 mm2 pieces and enzymatically digested with pa apain digestion kit (Worthington, LK003163) with the addition of 0.5 mg/ml DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Developmental Biology 2023Quote: Two organoids per experimental condition were washed with Ca2+/Mg2+-free DPBS and cut into 1 mm2 pieces and enzymatically digested with papain digestion kit (Worthington, LK003163) with the addition of DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Immunology 2022Quote: ... isolated cells were cultured for 24 h with ovalbumin protein (Worthington, LS003048) at 500 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Recipients were immunised with 100µL of 1µg/mL of OVA protein (Worthington Biochemicals) in Freund’s complete adjuvant (CFA ...
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bioRxiv - Developmental Biology 2019Quote: ... Papain from Worthington kit (Worthington Biochemical Corporation, LK003150) was added and cortices were placed on a rotator at 37 °C for 30 minutes (E11.5 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... Proteins were released from the DNA by incubation with 5 U of DNA I (Worthington) in 10 mL of 25 mM HEPES-KOH (pH 7.6) ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... 0.5 mM EDTA (Worthington Biochem kit), and 0.01% DNase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The Papain Dissociation Kit (Cat# LK003150 Worthington) was used to dissociate the tissue ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Dissociation medium was aspirated until 1 mL remained and an activated papain solution (1:1 papain [Worthington-Biochem] with 13.6 mM Cysteine-HCL ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1:1 DMEM:F12K supplemented with DNase I (Worthington Biochemical), 6.5mg/ml collagenase (Worthington Biochemical) ...
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bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
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bioRxiv - Immunology 2020Quote: ... for a total of 7 days starting two days prior to immunisation with 40μg of ovalbumin (OVA) protein (Worthington) conjugated to 20μm polyethylene carboxylate beads (Polysciences Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... prepared using 1:1 albumin-ovomucoid solution (10mg/ml of each) (Worthington, Cat# OI ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour (Patient #1) or 3.7mg/mL collagenase II (Worthington, LS004177) for 90 minutes followed by 6mg/mL dispase (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 1 mg/ml each of Collagenase Type 1 (Worthington Biochemical LS004216), Type 2 (Worthington Biochemical LS004204) ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004216, 5x50 mg, $200.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and 1% DNase (Worthington) at 37°C for 6 minutes at which point DMEM (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... Dissociation was performed using papain dissociation kit (Worthington Biochemical) for 20 minutes at 37°C and stopped using DMEM + 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... papain (PDS Kit; LK003178; Worthington Biochemical Corporation, Lakewood, NJ), DNase I (90083 ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... Processing was done using the Papain kit from Worthington, and cells were counted and diluted to a density of 5 Million per ml ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi were dissociated using papain dissociation kit (Worthington LK003176) for 10 minutes at 30°C ...
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bioRxiv - Biochemistry 2024Quote: ... Lungs from 4 mice were minced into ∼1 mm pieces using curved scissors and digested with 1 mg/ml collagenase type 1 (Worthington, cat# LS004194) and 1X dispase (Corning ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask