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Citations for Worthington Biochemical :
201 - 250 of 272 citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Approximately 10~15 mg of proteins were then used for a sequential digestion using lysyl endopeptidase (Lys-C; Wako Pure Chemical Corp., Osaka, Japan) and trypsin (Worthington Biochemical Corp., Lakewood, NJ, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... the digestion was stopped by a solution of trypsin inhibitors (Leibovitz’s L15 medium, 10% 1 mg/ml trypsin inhibitor [Worthington], and 2% 10 mg/ml DNAse) and cells were washed with L15 medium ...
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bioRxiv - Pathology 2021Quote: ... Retained proteins were digested with trypsin (Worthington), at a ratio of 10:1 protein to trypsin ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with trypsin (Worthington-Biochem) in 50 mM TEAB pH 8.0 at an enzyme-to-protein ratio of 1:10 (w/w ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were digested by adding bovine trypsin (Worthington-LS003750) and endoproteinase Lys-C (NEB-P8109S ...
Product cited:
Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl...Cat# LS003750, 100 mg, $170.00 Ask -
bioRxiv - Immunology 2022Quote: ... isolated cells were cultured for 24 h with ovalbumin protein (Worthington, LS003048) at 500 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Recipients were immunised with 100µL of 1µg/mL of OVA protein (Worthington Biochemicals) in Freund’s complete adjuvant (CFA ...
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bioRxiv - Developmental Biology 2019Quote: ... Papain from Worthington kit (Worthington Biochemical Corporation, LK003150) was added and cortices were placed on a rotator at 37 °C for 30 minutes (E11.5 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... Proteins were released from the DNA by incubation with 5 U of DNA I (Worthington) in 10 mL of 25 mM HEPES-KOH (pH 7.6) ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... 0.5 mM EDTA (Worthington Biochem kit), and 0.01% DNase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The Papain Dissociation Kit (Cat# LK003150 Worthington) was used to dissociate the tissue ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Immunology 2020Quote: ... for a total of 7 days starting two days prior to immunisation with 40μg of ovalbumin (OVA) protein (Worthington) conjugated to 20μm polyethylene carboxylate beads (Polysciences Inc. ...
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bioRxiv - Developmental Biology 2021Quote: ... Dissociation was performed using papain dissociation kit (Worthington Biochemical) for 20 minutes at 37°C and stopped using DMEM + 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... papain (PDS Kit; LK003178; Worthington Biochemical Corporation, Lakewood, NJ), DNase I (90083 ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... Processing was done using the Papain kit from Worthington, and cells were counted and diluted to a density of 5 Million per ml ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi were dissociated using papain dissociation kit (Worthington LK003176) for 10 minutes at 30°C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... GFP+ cortices were dissociated using a papain dissociation kit (Worthington) and trituration using glass pipettes (BrainBits LLC) ...
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bioRxiv - Neuroscience 2023Quote: ... and DNAse in 1x EBSS (all from Worthington, kit LK003150). This cell suspension was gently applied on top of a 5 mL solution of 10 mg/mL ovomucoid inhibitor ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Using a commercially available papain tissue dissociation kit (Worthington Biochemical, LK003150), hippocampi are transferred to Earle’s Balanced Salt Solution (EBSS)-based papain solution supplemented with DNase in a 6cm petri dish pre-equilibrated in 5% CO2 36°C incubator and chopped for 30 seconds with Bonn scissors before being returned to incubator for a 10-to 40-minute incubation commiserate with pup age ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... the tissues were transferred in PDS Kit-Inhibitor solution (#LK003182, Worthington). Tissue chunks were triturated ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Neuroscience 2020Quote: The forebrain was dissociated by the papain dissociation kit (LK003176, Worthington) and dissociator (Miltenyi Biotec ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Genomics 2023Quote: ... Cells were dissociated using the Papain cell dissociation kit (Worthington, LK003150). Chromium 3’ single-cell RNA-seq kit (10xGenomics ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2024Quote: ... Papain was inactivated using an Ovomucoid inhibitor (Worthington Papain Dissociation Kit) and dissociated cells were passed through a 20 μm cell strainer ...
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bioRxiv - Neuroscience 2024Quote: ... Isolated tissues underwent dissociation using a papain dissociation kit (#LK003176, Worthington) supplemented with DNase I (250 U/ml ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... The isolated cortical tissues were dissociated by papain dissociation kit (#LK003176, Worthington) supplemented with DNase I (250U/ml ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Molecular Biology 2022Quote: ... The reaction was inactivated with Ovomucoid (Papain dissociation kit, Worthington Biochemical Corporation). Cells were centrifuged for 5 minutes at 400g ...
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bioRxiv - Developmental Biology 2019Quote: ... Tissues were dissociated into single cells using the Papin Dissociation Kit (Worthington) as described previously 47 ...
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bioRxiv - Cancer Biology 2019Quote: Xenografted tumors were dissected and mechanically dissociated using papain dissociation kits (Worthington Biochemical Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... the papain dissociation kit was used according to the manufacturer’s protocols (Worthington). Briefly ...
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bioRxiv - Genomics 2023Quote: ... Cells were separated with a papain dissociation kit (Worthington, cat. no. #LK003150) with minor modifications [87] ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... tissue was enzymatically digested with the Papain Dissociation System kit (Worthington, LK003176) at 35°C for 90 minutes with 95% O2 ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... Tissue was enzymatically digested with the Papain Dissociation System kit (Worthington, LK003176), using 1 vial (>100 units ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Cancer Biology 2019Quote: Xenografted tumors were dissected and mechanically dissociated using a papain dissociation kit (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... Half cerebella were processed using the Cell Dissociation Kit (Worthington Biochemical Corporation, #LK003150), in which samples were treated with papain at 37°C for 15min and then separated by centrifugation of an ovomucoid inhibitor density gradient ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Tumors samples were dissociated using the Cell Dissociation Kit (Worthington Biochemical Corporation, #LK003150), which included dissociation with papain at 37°C for 15min ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Hippocampal regions were dissected and dissociated with the Worthington Papain Dissociation Kit (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue chunks were then transferred to the PDS Kit-Inhibitor solution (#LK003182, Worthington) and filtered through strainers ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Genetics 2020Quote: ... cells were dissociated from transwells with a papain dissociation kit (Worthington Biochemical, Cat# LK003150) and filtered using a Flowmi cell strainer (Bel-Art SP Scienceware ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... by sectioning the optic nerves and were dissociated using the papain kit from Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were isolated from cortical tissue using the papain dissociation kit (Worthington Biochemical Inc.) as previously described [24] [25] and cells were plated at a density of 175,000 cells/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... and 250U ml−1 DNase in EBSS using the Papain Dissociation Kit (Worthington, Cat #:LK003150). The tissue was mechanically triturated and suspended cells were isolated and resuspended in EBSS with albumin-ovomucoid inhibitor solution ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2019Quote: ... single cells were prepared from the bulk tumor by a Papain dissociation kit (Worthington Biochemical) as per the manufacturer’s protocol and cultured using previously reported culturing methods76 ...
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bioRxiv - Cancer Biology 2021Quote: ... E15-P30 Cerebella were dissociated with a Papain dissociation kit following the manufacturer’s instructions (Worthington). Following papain treatment ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: Retinas were dissociated into single-cell suspensions using a papain dissociation kit (Worthington, Columbus, OH). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... modified to use early post-natal mice and to employ a commercial kit (Worthington Biochemical, LK003150). P1-P4 mice are sacrificed by guillotine method cut just caudal to the ears ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2024Quote: Cerebral organoids were dissociated into a single-cell suspension using Worthington Papain System kit (Worthington, LK003150) according to the manufacturer’s instruction ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... Total tumor collected per mouse was dissociated using standard methods with a Papain dissociation kit (Worthington Biologicals). Following filtration through a 40 micron filter ...