Labshake search
Citations for Worthington Biochemical :
1 - 50 of 430 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade B 89BZ_167 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The RT units of a concentrated stock of HIV Q23.BG505 virus were determined multiple times using a standard curve of purified HIV RT enzyme (Worthington Biochemical Corp. #LS05003). Aliquots of this titered stock of Q23.BG505 were used as the quantitative standard curve in all assays ...
Product cited:
Chromatographically purified dimeric form with M.W. of 66 kDa and 51 kDa. A solution in 10 mM...Cat# LS05003, 200 un, $95.00 Ask -
bioRxiv - Molecular Biology 2022Quote: ... Recombinant HIV (Worthington) and HiScript III (Vazyme ...
-
bioRxiv - Biochemistry 2020Quote: Reverse transcription reactions with Moloney Murine Leukemia Virus (MMLV) RT (BioBharati Life Sciences Pvt. Ltd) and HIV RT (Worthington Biochemical) were carried out according to the manufacturer’s directions using primer P5 (Table S1) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were incubated with 1 mg/ml collagenase B (Worthington Biochemical) for 1 hour at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
-
bioRxiv - Biochemistry 2020Quote: ... 5 U SUPERase•In™ RNase Inhibitor and 5 U HIV RT (Worthington Biochemical Corporation, cat. HIVRT). For TGIRT-III ...
-
bioRxiv - Physiology 2021Quote: ... beating cardiomyocytes were detached from plates with 1 mg/ml collagenase B (Worthington Biochemical), dissociated with 0.25% Trypsin/EDTA (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2019Quote: ... tissue supernatants were serially diluted 10-fold in virus infection medium supplemented with 1 µg/mL L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ). The plates were rinsed twice with PBS ...
-
bioRxiv - Bioengineering 2020Quote: ... Infection with the H1N1 virus was performed in the presence of 1 µg of TPCK (tolylsulfonyl phenylalanyl chloromethyl ketone)-trypsin/ml (Worthington Biomedical).27 Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... virus was activated by adding 8μg/mL trypsin (TPCK treated, Worthington, Lakewood, NJ) in FCS-free media to samples in a 1:1 ratio ...
-
bioRxiv - Systems Biology 2020Quote: ... The dissociation was carried out in dissection buffer containing collagenase B (1-2 mg/ml) and hyaluronidase (1.2-2 mg/ml, Worthington Biochemical) at 37 °C with frequent agitation for 30 min ...
-
bioRxiv - Physiology 2022Quote: Human adipocytes were isolated from adipose tissue samples by collagenase (type 1, Worthington, NJ, USA) as described previously [24] ...
-
bioRxiv - Microbiology 2021Quote: ... At pre-determined timepoints post-infection 100μL of PBS (or in the case of influenza virus PBS + 0.1 μg of TPCK-treated trypsin (Worthington, USA)) was added to the apical compartment and cells were incubated at 37°C with 5% CO2 for 10 minutes ...
-
bioRxiv - Neuroscience 2020Quote: Primary human cortical tissue was dissociated with Papain (Worthington)-containing DNase ...
-
bioRxiv - Neuroscience 2023Quote: ... Human samples were dissociated according to a modified papain-based protocol (Worthington), then filtered through a 40 µM filter before counting and dilution for scRNAseq.
-
bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
-
bioRxiv - Pathology 2021Quote: ... Retained proteins were digested with trypsin (Worthington), at a ratio of 10:1 protein to trypsin ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with trypsin (Worthington-Biochem) in 50 mM TEAB pH 8.0 at an enzyme-to-protein ratio of 1:10 (w/w ...
-
bioRxiv - Biochemistry 2024Quote: ... epidydimal fat from 4-month-old male DIO C57BL/6J mice was dissected out and dissociated using 2 mg/ml Collagenase B (Worthington, CLSAFB) and 1 mg/ml soybean trypsin inhibitor (Worthington ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were digested by adding bovine trypsin (Worthington-LS003750) and endoproteinase Lys-C (NEB-P8109S ...
Product cited:
Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl...Cat# LS003750, 100 mg, $170.00 Ask -
bioRxiv - Immunology 2019Quote: Human Omentum was weighed and 0.035 – 1.8g of tissue was digested using 2mg/ml Collagenase I (Worthington) in PBS (Invitrogen/sigma ...
-
bioRxiv - Immunology 2022Quote: ... isolated cells were cultured for 24 h with ovalbumin protein (Worthington, LS003048) at 500 μg/ml ...
-
bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Paleontology 2019Quote: ... Hyaline articular cartilage was dissected and human chondrocytes isolated using 3 mg/mL Collagenase Type II (Worthington Cat # LS004177) in DMEM medium (Sigma-Aldrich ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2021Quote: ... Recipients were immunised with 100µL of 1µg/mL of OVA protein (Worthington Biochemicals) in Freund’s complete adjuvant (CFA ...
-
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... Proteins were released from the DNA by incubation with 5 U of DNA I (Worthington) in 10 mL of 25 mM HEPES-KOH (pH 7.6) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Human iPSC-CMs used for patch clamp measurements were dissociated from EHT by collagenase II (200 U/mL; Worthington Biochemical, Lakewood, NJ, USA) for 5 h ...
-
bioRxiv - Neuroscience 2023Quote: ... Dissociation medium was aspirated until 1 mL remained and an activated papain solution (1:1 papain [Worthington-Biochem] with 13.6 mM Cysteine-HCL ...
-
bioRxiv - Developmental Biology 2023Quote: ... in 1:1 DMEM:F12K supplemented with DNase I (Worthington Biochemical), 6.5mg/ml collagenase (Worthington Biochemical) ...
-
bioRxiv - Physiology 2021Quote: ... The proteins were digested for 5 hours with Lys-C (Wako) at room temperature and overnight with trypsin (Worthington). Samples were acidified and diluted with TFA to a final concentration of 1% TFA before being loaded on the Sep-Pak Light C18 cartridges (Waters) ...
-
bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Immunology 2020Quote: ... for a total of 7 days starting two days prior to immunisation with 40μg of ovalbumin (OVA) protein (Worthington) conjugated to 20μm polyethylene carboxylate beads (Polysciences Inc. ...
-
bioRxiv - Neuroscience 2020Quote: ... prepared using 1:1 albumin-ovomucoid solution (10mg/ml of each) (Worthington, Cat# OI ...
-
bioRxiv - Genomics 2023Quote: ... for 1 hour (Patient #1) or 3.7mg/mL collagenase II (Worthington, LS004177) for 90 minutes followed by 6mg/mL dispase (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... containing 1 mg/ml each of Collagenase Type 1 (Worthington Biochemical LS004216), Type 2 (Worthington Biochemical LS004204) ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004216, 5x50 mg, $200.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and 1% DNase (Worthington) at 37°C for 6 minutes at which point DMEM (Gibco ...
-
bioRxiv - Biochemistry 2024Quote: ... Lungs from 4 mice were minced into ∼1 mm pieces using curved scissors and digested with 1 mg/ml collagenase type 1 (Worthington, cat# LS004194) and 1X dispase (Corning ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Genetics 2020Quote: ... 1 mg ml-1 soybean trypsin inhibitor (#LS003570, all purchased from Worthington Biochemical Corporation), and 1% penicillin/streptomycin in HBSS for 10 min at 37°C with gentle shaking ...
Product cited:
Chromatographically purified as described by Frattali and Steiner, Biochem., 7, 521 (1968). A...Cat# LS003570, 100 mg, $74.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... papain (1 mg/ml, Worthington), ethylenediaminetetraacetic acid (EDTA ...
-
bioRxiv - Immunology 2023Quote: ... 1 mg/ml hyaluronidase (Worthington), 0.1 mg/ml DNase I (Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... This solution was then replaced with 1mg/ml collagenase 1 (Worthington Biochemical Corporation CLS-1) in 0.1M PBS and 20mM HEPES for 60-90 min at 37°C followed by 0.05% Trypsin-EDTA (Sigma T9201 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were spun down and supernatant was removed before the addition of 1 ml Dispase-mUE (Dispase II [7 mg ml-1 DMEM Ham’s F12], Neutral Protease, Worthington NPRO2; and 10 μg μL-1 DNase). Cells were aspirated in the solution gently 40 times using a 1 ml pipette tip ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 ml Trypsin (1 g/ml)-DNase (100 mg/ml) solution (Worthington Biochemical, 3703 and 2139) was added to the pellet for 1.5 min at 37°C without disturbing the pellet ...
-
bioRxiv - Microbiology 2023Quote: ... and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals). Plaque forming units (PFU ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml trypsin inhibitor (Worthington), 2 % (v/v ...
-
bioRxiv - Physiology 2019Quote: ... 2.5mg/ml collagenase Type 1 (Worthington) made up in low Ca2+/low Mg2+ HBSS ...
-
bioRxiv - Immunology 2022Quote: ... dispase (0.1 U ml−1; Worthington) and 10% FBS ...
-
bioRxiv - Physiology 2022Quote: ... 2.5mg/ml collagenase Type 1 (Worthington) made up in low Ca2+/low Mg2+ HBSS ...
-
bioRxiv - Microbiology 2021Quote: ... 30 mg ml-1 DNase (Worthington), and 1 mM MgCl2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Deoxyribonuclease 1 (Cat: LS002139, Worthington). Next ...
Product cited: